O ambiente virtual Moodle como espaço de articulação e organização docente

Propõe o plano de desenvolvimento e a construção de um protótipo de ação docente de Coordenação Pedagógica estruturada em ambiente virtual Moodle e a análise do trabalho realizado.33 páginasEducação e DocênciaNível: Micro - Ensino e Aprendizagem na Educação a Distância Área Temática: Interação e comunicação em comunidades de aprendizage

Gestão Escolar: Enfoques Teóricos, Concepções e Formação Docente

A gestão escolar é um trabalho de mediação com vistas ao cumprimento das finalidades educacionais, as quais podem assumir sentido destoante de uma orientação filosófica emancipadora. A reflexão sobre a gestão escolar torna-se importante contribuição aos processos de formação docente para o exercício desse trabalho. Entendemos que, sem a reflexão teórica e crítica, a gestão assume um caráter praticista e burocrático, alinhando-se facilmente à lógica neoliberal. Assim, a partir de uma perspectiva crítica, refletimos sobre a gestão escolar, abordando os enfoques teóricos e as concepções a eles associados e aspectos relevantes da formação docente para a efetivação da práxis educativa

O Discurso Filosófico como Legitimação do Poder e Formação do Estado

O artigo aborda a genealogia do conhecimento como maneira de compreensão do discurso filosófico que embasa a legitimação do poder e a formação do Estado. A participação popular deve estar pautada pela melhor organização da máquina pública, representando de forma cristalina os anseios da sociedade e conferindo maior segurança às decisões políticas a serem tomadas. Ademais, o artigo que aqui se apresenta tem um viés exploratório e descritivo, fundamentado em uma abordagem qualitativa, a qual partiu da análise de documentos e de observações da realidade que foram confrontados com a recente doutrina sobre o tema e a legislação vigente

Prevalence and influence of overweight and obesity on clinical and epidemiological profile of breast cancer patients in the North of Mato Grosso, Brazil: a retrospective cross-sectional study

Obesity is one of the main preventable risk factors in post-menopausal breast cancer. This retrospective cross-sectional study aimed to demonstrate the clinical and epidemiological profile of breast cancer patients diagnosed in the period from 2013 to 2018 in the North of Mato Grosso, Brazil and to verify the prevalence and influence of overweight and obesity in these patients. Data were collected from patient’s medical records who were diagnosed with breast carcinoma in the Department of Oncology of Santo Antônio’s Hospital, in Sinop-MT. 196 patients were included. 99.5% were women. The majority were married, ≥50 years old (57.7%) and overweight or obese. In the overweight and obesity group the percentage of patients with invasive breast carcinoma were significantly higher when compared with eutrophic group (p=0.03). In all groups the profile of estrogen and progesterone receptors positive and HER-2 negative were more prevalent, however, the frequency of triple negative profile was higher in the overweight (7.1%) and obesity (6.3%) group when compared with control (4.3%) group, as well as the presence of hypertension and diabetes. In conclusion, it was observed a high prevalence of overweight and obesity in breast cancer patients, which contributed to modify the histological type of breast cancer (high prevalence of invasive and lobular carcinomas), increase the frequency of patients in stages 3 and 4, the percentage of triple negative profile and the frequency of other comorbidities, as hypertension and diabetes. Furthermore, metformin, an antidiabetic drug, seems to be contributing to reduce tumor development and improve the clinical profile and prognosis in diabetic breast cancer patients

Mortality due to malignant neoplasms of the liver and intrahepatic biliary tract in the state of Mato Grosso in 2020

Malignant neoplasms of the liver and intrahepatic biliary tract occupy the third place of cancer deaths in the world and the second most deadly in men and the sixth in women. Eating habits characterized by the consumption of food with high caloric and glycemic indexes, associated with a sedentary lifestyle, provide a greater risk of developing hepatic steatosis, as well as obesity, which, together with the increased incidence of chronic viral hepatitis, excessive alcoholism, and age over 50 years, constitute the main risk factors for the development of these types of cancer. In addition, due to the chronicity of liver pathologies, the diagnosis is usually late, a factor that contributes to increase the mortality rate. Thus, the objective of this work is to analyze the epidemiological profile of deaths due to liver cancer and intrahepatic biliary tract that occurred in the state of Mato Grosso in 2020. This is a cross-sectional study based on data from the Mortality Information System (SIM), made available by the Department of Informatics of the Unified Health System (DATASUS), and in the population estimate of the Brazilian Institute of Geography and Statistics (IBGE) for 2020. A study was carried out of the epidemiological profile of deaths related to liver and intrahepatic biliary ducts neoplasms (CID 10 C-22) of the state of Mato Grosso for the year 2020 regarding age group, sex (gender), and race. A total of 120 deaths due to malignant neoplasms of the liver and intrahepatic biliary tract were reported, corresponding to a mortality rate of approximately 3.4 deaths/100,000 inhabitants, with the proportion of deaths between men and women approximately 4:3. For both sexes, there is an abrupt increase in the incidence of deaths after the sixth decade of life, corresponding to 90% of deaths due to this pathology. Regarding the racial evaluation, deaths are more incident in the yellow race, with about 4.97 deaths/100,000 inhabitants, followed by the black race with 4.87 deaths/100,000 inhabitants. Thus, it is evident that deaths from liver and intrahepatic biliary tract malignant neoplasms in the state of Mato Grosso, in 2020, were concentrated from the sixth decade of life, especially in men, with emphasis on the yellow race. It should be noted that the analysis of epidemiological characteristics is essential for the development of screening policies and early diagnosis in patients with modifiable and non-modifiable risk factors

An Acidic Thermostable Recombinant Aspergillus nidulans Endoglucanase Is Active towards Distinct Agriculture Residues

Aspergillus nidulans is poorly exploited as a source of enzymes for lignocellulosic residues degradation for biotechnological purposes. This work describes the A. nidulans Endoglucanase A heterologous expression in Pichia pastoris, the purification and biochemical characterization of the recombinant enzyme. Active recombinant endoglucanase A (rEG A) was efficiently secreted as a 35 kDa protein which was purified through a two-step chromatography procedure. The highest enzyme activity was detected at 50 ∘ C/pH 4. rEG A retained 100% of activity when incubated at 45 and 55 ∘ C for 72 h. Purified rEG A kinetic parameters towards CMC were determined as = 27.5 ± 4.33 mg/mL, max = 1.185 ± 0.11 mmol/min, and 55.8 IU (international units)/mg specific activity. Recombinant P. pastoris supernatant presented hydrolytic activity towards lignocellulosic residues such as banana stalk, sugarcane bagasse, soybean residues, and corn straw. These data indicate that rEG A is suitable for plant biomass conversion into products of commercial importance, such as second-generation fuel ethanol

Clonagem, expressão heteróloga, purificação e caracterização funcional da endoglicanase A de Aspergillus nidulans

Dissertação (mestrado)—Universidade de Brasília, Instituto de Ciências Biológicas, Departamento de Biologia Molecular, 2010.O Bioetanol é um combustível alternativo particularmente interessante, pois paralelamente às técnicas já estabelecidas pela indústria sucroalcooleira no Brasil, são geradas imensas quantidades de bagaço-de-cana, um resíduo bastante sub-aproveitado. Este pode apresentar de 40 a 50% de celulose, polímero resultante da repetição de unidades de Dglicose unidas por ligações ß-1,4. Estas podem ser alvo de enzimas celulolíticas produzidas principalmente por fungos filamentosos. O emprego de estratégias envolvendo sistemas heterólogos para produção destas enzimas pode favorecer o alcance dos níveis máximos de produção em prazos inferiores aos observados em sistemas nativos, além de facilitar a purificação da proteína recombinante. Neste trabalho, foi realizada a caracterização parcial da endoglicanase A (EG A) de Aspergillus nidulans, enzima de interesse biotecnológico incluída no projeto da rede Bioetanol (MCT/FINEP), onde nosso grupo desenvolve a meta de “Produção de celulases recombinantes em sistema heterólogo de Pichia pastoris”. As construções para clonagem envolveram promotores induzíveis por metanol, sendo o vetor de expressão obtido dirigido para integração no genoma de P. pastoris. Assim, após clonagem e transformação desta levedura metilotrófica, foi realizada a expressão heteróloga desta enzima seguida de sua purificação parcial. Empregando o sobrenadante dos clones recombinantes, foi obtido um produto heterólogo com atividade máxima em carboximetilcelulose (CMC) a partir de 24 h de indução. Após purificação empregando ultrafiltração e filtração em gel, a enzima recombinante foi caracterizada, atuando preferencialmente em pH ácido (de 4,5 a 5,0) e temperatura próxima a 50oC, em torno da qual esta enzima apresentou-se termoestável a 45 e 55oC por cerca de 48 h. Foi observado um incremento de 30 a 80% nos valores de atividade diante da incubação com Co2+, DTT e ß-mercaptoetanol. A degradação de substratos alternativos mostrou-se significativa somente na incubação com CMC e, em menor extensão, em papel de filtro. Além disso, a análise de atividade em gel e o perfil eletroforético das amostras das etapas da purificação confirmaram a massa molecular de 34 kDa, conforme esperado. _________________________________________________________________________________ ABSTRACTBioethanol is an alternative fuel of particular interest because in Brasil, the techniques used in fuel industry generate an enormous amount of a by-product, which could be further degraded: the sugarcane bagasse. It can presents 40-50% of cellulose content, which is a polymer composed by repeated units of D-glucose, linked by ß-1,4 bonds. These bonds can be cleaved by cellulolytic enzymes produced specially by filamentous fungi. The use of heterologous expression strategies to produce these enzymes can be advantageous in order to higher levels of production in shorter periods compared as those observed with wild type enzymes, besides the feasibility of recombinant protein purification methods. In this work, we describe the partial characterization of endoglucanase A (EG A), produced by Aspergillus nidulans. On account of the biotechnological interest, this enzyme was included in the project of the Bioethanol net (MCT/FINEP), and our group was responsible for developing the “Recombinant cellulases production employing Pichia pastoris as a heterologous expression system. The cloning vector was constructed using methanol-induced promoters, with the foreign DNA being directed to be integrated in the Pichia pastoris genome. Finally the enzyme was partially purified and characterized. To examine whether higher levels of activity towards CMC would occur, we used the supernatant of the recombinant clones. We observed the highest level of activity after 24h of induction. After ultrafiltration and gel filtration purification procedures, we characterized an enzyme that acts preferentially at pH 4, which is thermostable at a temperature of approximately 50ºC. It was observed an increased enzyme activity when the enzyme was incubated in the presence of Co2+, DTT and ß-mercaptoethanol. The hydrolysis of lignocellulosic substrates was significant only with CMC and, at a lesser extent, with filter paper. Also, the analysis of activity in gel and the electrophoretic profile of the purified samples confirmed the molecular mass of 34 kDa, as predicted