Galectin-3 is an important mediator of VEGF- and bFGF-mediated angiogenic response

Recent studies have shown that a carbohydrate-binding protein, galectin-3, is a novel pro-angiogenic molecule. The mechanism by which galectin-3 promotes angiogenesis remains unknown. We demonstrate here that galectin-3 is a mediator of vascular endothelial growth factor (VEGF)- and basic fibroblast growth factor (bFGF)-mediated angiogenic response. Angiogenesis assays revealed that galectin-3 inhibitors, β-lactose and dominant-negative galectin-3, reduce VEGF- and bFGF-mediated angiogenesis in vitro and that VEGF- and bFGF-mediated angiogenic response is reduced in galectin-3 knockdown cells and Gal3−/− animals. Integrin αvβ3 was identified as the major galectin-3–binding protein and anti-αv, -β3, and -αvβ3 integrin function-blocking antibodies significantly inhibited the galectin-3–induced angiogenesis. Furthermore, galectin-3 promoted the clustering of integrin αvβ3 and activated focal adhesion kinase. Knockdown of GnTV, an enzyme that synthesizes high-affinity glycan ligands for galectin-3, substantially reduced: (a) complex N-glycans on αvβ3 integrins and (b) VEGF- and bFGF-mediated angiogenesis. Collectively, these data suggest that galectin-3 modulates VEGF- and bFGF-mediated angiogenesis by binding via its carbohydrate recognition domain, to the GnTV synthesized N-glycans of integrin αvβ3, and subsequently activating the signaling pathways that promote the growth of new blood vessels. These findings have broad implications for developing novel, carbohydrate-based therapeutic agents for inhibition of angiogenesis

An Elusive Legacy: The Rediscovery of Roman Baths in Eighteenth-Century Britain

In this paper, I investigate how eighteenth-century antiquarians engaged with the remains of Roman bath buildings in Britain and discuss their multifaceted attitude towards the ancient practice of bathing, with a focus on the city of Bath. I also examine the interests and priorities of Georgian scholars in studying Roman baths and their structure, highlighting their sometimes uncritical use of Classical sources and tracking the origins of their misconceptions regarding the components and function of these facilities. Finally, I briefly address the elusive socio-cultural legacy of Roman baths and bathing in eighteenth-century Britain, stressing influences and differences in practice and architecture

Artificial coastal lagoons at solar salt-working sites: A network of habitats for specialised, protected and alien biodiversity

There are concerns that novel structures might displace protected species, facilitate the spread of nonindigenous species, or modify native habitats. It is also predicted that ocean warming and the associated effects of climate change will significantly increase biodiversity loss within coastal regions. Resilience is to a large extent influenced by the magnitude of dispersal and level of connectivity within and between populations. Therefore it is important to investigate the distribution and ecological significance of novel and artificial habitats, the presence of protected and alien species and potential vectors of propagule dispersal. The legacy of solar salt-making in tropical and warm temperate regions is regionally extensive areas of artificial hypersaline ponds, canals and ditches. Yet the broad-scale contribution of salt-working to a network of benthic biodiversity has not been fully established. Artisanal, abandoned and historic salt-working sites were investigated along the Atlantic coast of Europe between southern England (50 N) and Andalucía, Spain (36 N). Natural lagoons are scarce along this macrotidal coast and are vulnerable to environmental change; however it is suspected that avian propagule dispersal is important in maintaining population connectivity. During bird migration periods, benthic cores were collected for infauna from 70 waterbodies across 21 salt-working sites in 5 coastal regions. Bird ringing data were used to investigate potential avian connectivity between locations. Lagoonal specialist species, some of international conservation importance, were recorded across all regions in the storage reservoirs and evaporation ponds of continental salinas, yet few non-indigenous species were observed. Potential avian propagule transport and connectivity within and between extant salt-working sites is high and these artificial habitats are likely to contribute significantly to a network of coastal lagoon biodiversity in Europ

Galileo: a very short introduction

In a startling reinterpretation of the evidence, Stillman Drake advances the hypothesis that Galileo's trial and condemnation by the Inquisition was caused not by his defiance of the Church, but by the hostility of contemporary philosophers. Galileo's own beautifully lucid arguments are used to show how his scientific method was utterly divorced from the Aristotelian approach to physics in that it was based on a search not for causes but for laws. Galileo's method was of overwhelming significance for the development of modern physics, and led to a final parting of the ways between science an

Mathematics and discovery in Galileo's physics

AbstractGalileo's steps in the discovery of the law of free fall and its application to inclined planes are retraced from one of his letters and some manuscript notes. Proofs of two preliminary theorems are reconstructed, and his methods of calculation are analyzed. Eudoxian proportion theory, and not mean-speed analysis, was the foundation of Galileo's work on motion