Correlation of Visualized Glucocorticoid Receptor and Apoptosis In Individual Clones of Nb2 Cells

The Nb2 lymphoma cell line is susceptible to glucocorticoid (GC)-induced apoptosis in the absence of mitogen and this effect is inhibited by the mitogen prolactin (PRL). This process is believed to be mediated by the glucocorticoid receptor (GR) and various downstream signals. The purpose of this study was to use the limiting dilution method to isolate subsets of Nb2 clones which expressed either highly enriched amounts of GR or an absence of GR and to relate the amount of GR to the magnitude of GC induced cytolysis. The presence of GR in these clones was determined by immunocytochemistry (ICC) using an affinity purified polyclonal rabbit antibody directed towards the GR. The presence of GR was then correlated to cytolysis in these cells produced by incubation with dexamethasone (Dex), a synthetic GC, in the presence and absence of PRL. Cytolysis was measured by two different methods, Trypan Blue exclusion counts, to determine the percentage of dead and the TUNEL (TdT-dependent dUTP-biotin Nick End Labeling) assay used to label apoptotic cells. The limiting dilution produced 28 clones with varying amounts of GR and all were responsive to Dex and this response was inhibited by PRL. A significant correlation (r2=0.419 and

Annexin A1 as a new functional linker between actin filaments and phagosomes during phagocytosis

In the current study annexin A1 was identified as a factor, which binds to latex beads phagosomes (LBP) and facilitates F-actin-LBP interaction in vitro. In macrophages the association of endogenous annexin A1 with LBP membranes is strongly correlated with F-actin accumulation at the LBP. Furthermore, it showed that receptor signalling influenced annexin A1 recruitment on different LBP. Reduction of annexin A1 expression resulted in impaired phagocytosis and actin flashing. These data revealed that annexin A1 as an important component of opsonic phagocytosis

An observational study of abnormal findings in fetus during 11-13+6 weeks and targeted imaging for fetal anomalies ultrasonography

Background: Congenital anomalies are responsible for a of 14.2% of perinatal mortality in India. This study focuses on incidence of structural fetal abnormalities detected during 1st and 2nd trimester sonography. Aim of this study was to evaluate the need for targeted fetal scans.Methods: This study is an observational study conducted at Gujarat fetal medicine Centre, Ahmedabad, Gujarat, super specialty antenatal radiology centre where exclusively antenatal USGs and prenatal procedures are performed. All pregnant female coming for 1st and 2nd trimester USG were included. All fetal abnormalities in form of soft markers and structural abnormality were included.Results: Out of 2122 total ANC scans, 183 cases (8.6%) had structural abnormality and 235 cases (11%) had significant soft markers. 1185 cases (55.84%) came in late 2nd trimester for TIFFA scan and 468 (22%) were seen in 1st trimester scan. The incidence of fetal anomaly in this study was higher than general population as it is an exclusively fetal medicine centre and majority of patients were diagnosed with anomaly in late 2nd trimester.Conclusions: There is immense need for early diagnosis and timely intervention before 20 weeks in case of prenatal detection of fetal abnormality

Development, Characterization of Hydroxyl Terminated Dendritic Macromolecules as Prospective Drug Carriers

The principal aspiration is to develop triazine dendrimers as potential drug carriers for sustained release. Triazine based dendrimer was synthesized by divergent method evading protection/ deprotection or functional group interconversion. Synthesized dendrimers were characterized by FTIR, 1H-NMR, 13C-NMR and ESI-Mass spectrometry. Synthesized full generation dendrimers G1, G2 and G3 were applied as solubility enhancers of hydrophobic drug ketoprofen. Ketoprofen was loaded by G3 dendrimer by inclusion complex method. Sustained release of ketoprofen from ketoprofen loaded dendrimer was studied and compared to of free ketoprofen. Cytotoxicity and hemolytic assay of dendrimer was studied to evaluate toxicity of dendrimer as drug vehicle

Characterization of the AP endonuclease enzyme APN-1 from C. elegans

Mémoire numérisé par la Division de la gestion de documents et des archives de l'Université de Montréal

Customized web-based data presentation

This is an electronic version of the paper presented at the World Conference on the WWW and Internet & Intranet (WebNet'98) held in Orlando, FL (United States) on 1998Reprinted from the WebNet 98 : World Conference of the WWW, Internet, & Intranet with permission of AACE (http://www.aace.org).This paper presents a language for specifying the presentation of data in Web pages. The language is an extension of HTML that includes constructs for specifying how to present one or more instances of a given class of data, and constructs for tailoring the presentation to the features of the data, to information in user profiles and to the capabilities of the user s platform. We describe the architecture of the system, the features of the page specification language, and present examples of generated pages

Prescription and cost-analysis of antiemetic medication use in pediatric wards: a prospective observational study

Background: Use of anti-emetic drugs in pediatric population is often warranted, but choice of drug remains questionable within pediatricians. Objective of current study is: to study prescribing pattern and to calculate cost of antiemetic drug therapy in pediatric wards.Methods: A prospective, observational study was conducted in pediatric wards of a tertiary care hospital of over 14 month’s duration. Institutional ethics committee approval was obtained and written informed consent of parents/guardians was taken. Data of any pediatric patient receiving anti-emetic agent were included in the study.Results: A total of 218 prescriptions were collected. Mean age of patients was 4.39±3.16 (range 4 months to 12 years). Gastroenteritis was the most frequently diagnosed disease in 137(63%) patients. Domperidone was prescribed in 52.4% and ondansetron in 47.6% children. Oral liquid dosage formulation was prescribed in 109 (48.4%) followed by solid dosage form 47 (20.9%). Mean cost of domperidone therapy was 25.34±6.55 INR and for ondansetron it was 36.62±17.94 INR.Conclusions: Gastroenteritis was most frequent indication for use of anti-emetics. Domperidone pharmacotherapy was cheaper and most frequently prescribed than ondansetron

A Survey on Human Activity Analysis Techniques

Human Activity Recognition(HAR) is Popular research topic in Computer vision and Image Processing area. This Paper Provide an exhaustive survey on the Entire Process of identify or Recognize Human activity. Basically, There are Four steps are involved in HAR process, which are Pre-processing, Feature extraction, Training, and Classification of different activities from video. The need of data preprocessing , and segmentation based on camera movements are presented. This paper provide detailed survey on different features for HAR, feature extraction and selection method , and Classification methods with advantages and disadvantages. Finally, A brief discussion about various classification techniques are presented

Comparison of analgesic, anti-inflammatory and anti-pyretic efficacy of diclofenac, paracetamol and their combination in experimental animals

Background: Various combinations of analgesics antipyretic drugs are available in the market for treatment of various musculoskeletal disorders and pain relief. Diclofenac and paracetamol combination is most commonly used combination and its rationality is questionable. The objective of this study was to compare the analgesic, anti-inflammatory and anti-pyretic activities of diclofenac, paracetamol and their combination.Methods: Experimental animals were divided into 4 different groups – control, diclofenac, paracetamol and their combination. Analgesic activity was compared by using tail-clip method in rats and writhing test in mice, anti-inflammatory activity was compared by carrageenan paw edema method using plethysmometer and anti-pyretic action was compared using TAB vaccine induced pyrexia and measuring the rectal temperature. Different doses of diclofenac (1mg/kg and 2mg/kg) and paracetamol (10mg/kg and 20mg/kg) used and same doses were used in combination group.Results: Diclofenac sodium (1mg/kg) showed significantly higher analgesic activity using tail-clip and writhing method compared to paracetamol (10mg/kg) (p<0.0001) and the combination group (p<0.05). Diclofenac sodium (2mg/kg) showed significantly higher analgesic activity using tail-clip and writhing method compared to paracetamol (20mg/kg) (p<0.0001) and the combination group (p<0.1). Diclofenac has no significant difference in anti-inflammatory activity using carrageenan induced paw edema when compared to the combination group (p<0.1) for both doses. But diclofenac when compared to paracetamol for anti-inflammatory effect, it was highly significant (p<0.0001 and p<0.0004) for both doses at 1st and 3rd hour. Paracetamol 20mg/kg was superior antipyretic (p<0.05,0.01 and 0.01 ) when compared to the combination group at 1hr, 2hr and 3hr duration after injecting TAB vaccine.Conclusion: Diclofenac and paracetamol combination was either equal or inferior in all three activities studied as compared to the individual drugs

Study of potential drug-drug interactions between benzodiazepines and four commonly used antiepileptic drugs in mice

Background: Benzodiazepines (BZD) is one of the commonly used drug groups for certain neurological diseases. As sometimes, the anti-epileptic drugs (AEDs) may be used concomitantly with BZD there is a potential for drug-drug interactions. Study aimed to study potential drug-drug interactions between four commonly used AEDs (phenytoin, carbamazepine (CBZ), phenobarbitone, sodium valproate) and BZD (diazepam, clonazepam) in mice using maximal electroshock seizure (MES) method and pentylenetetrazole (PTZ) method.Methods: Adult male albino mice were divided into four different groups of six animals each and anti-epileptic activity was assessed using MES method and PTZ method. Group I acted as a control, Group II received any one of the four AEDs (phenytoin, CBZ, phenobarbitone or sodium valproate) in sub-effective doses, Group III received diazepam or clonazepam alone, Group IV received a combination of diazepam or clonazepam with any one of the AEDs.Results: In MES method, the groups receiving combination of diazepam with phenytoin and CBZ showed significant protection compared to the control group (p<0.01 and p<0.02), respectively. However, diazepam in combination with sodium valproate and phenobarbitone did not show any significant protection compared to the control group and individual antiepileptic group. All the four antiepileptic showed significant protection against MES seizure in combination with clonazepam when compared to control group. In PTZ method, combination of sodium valproate with clonazepam showed significant protection compared to control group (p<0.02). However, this was not observed with diazepam-valproate combination.Conclusion: Clonazepam potentiates the action of all the four anti-epileptics while diazepam potentiates only phenytoin and CBZ against MES seizures. Clonazepam but not diazepam potentiates the action of sodium valproate against PTZ seizures