Pituitary hormone mRNA expression in European sea bass Dicentrarchus labrax in seawater and following acclimation to fresh water

Contains fulltext : 35512.pdf (publisher's version ) (Open Access)The mRNA expression of pituitary prolactin (prl), growth hormone (gh), somatolactin (sl), proopiomelanocortin (pomc), and gonadotropins (gthI and gthII) was quantified by real-time PCR, in sea bass, Dicentrarchus labrax, adapted for 1 month to seawater (SW) or freshwater (FW). In addition, IGF-I (igfI) mRNA expression in liver and branchial Na(+)/K(+)-ATPase activity were determined. L17 ribosomal protein (rpL17) and elongation factor 1alpha (ef1alpha) were validated as reference genes in real-time PCR in the experimental context. The real-time PCR assays were validated for the different hormone genes considered. Expression of pituitary pomc, gthI, gthII, gh, and liver igfI was not significantly different between FW and SW fish. Pituitary prlwas 4.5-foldhigher in FWthan in SW, whereas pituitary sl was 1.8-fold higher in SW- compared with FW-adapted fish. Gill Na(+)/K(+)-ATPase specific activity was 2.3-fold higher in FW sea bass compared with SW fish. Plasma cortisol levels were 6.5-fold lower in SW- than in FW-adapted specimens. The results are discussed in relation to the osmoregulatory strategy of this euryhaline SW species, which displays features that do not fit present models based on salmonids and FWeuryhaline teleosts

Pharmacogenomic analysis of acute promyelocytic leukemia cells highlights CYP26 cytochrome metabolism in differential all-trans retinoic acid sensitivity

U okviru ovog završnog rada objašnjavaju se osnovni principi rada hidrauličkih i pneumatskih sustava na obradnim strojevima. Rad detaljno objašnjava osnovne dijelove od kojih se sastoje ovi sustavi, te koja je njihova uloga u sustavu. Razrađeni su jednostavni primjeri dimenzioniranja ovih sustava, te na primjeru shema spoja pokazan je način spajanja i funkcije sustava.Within this final work, the bacis principles of hydraulic and pneumatic systems on processing machines have been explained. The final work explaines in detail the basic parts of which these systems are composed and what their roles in these systems are. Simple examples of dimensioning of these system have been elaborated, and the connection scheme and system function are shown in the example of the connection scheme

Kmerator Suite: design of specific k -mer signatures and automatic metadata discovery in large RNA-seq datasets

International audienceThe huge body of publicly available RNA-sequencing (RNA-seq) libraries is a treasure of functional information allowing to quantify the expression of known or novel transcripts in tissues. However, transcript quantification commonly relies on alignment methods requiring a lot of computational resources and processing time, which does not scale easily to large datasets. K-mer decomposition constitutes a new way to process RNA-seq data for the identification of transcriptional signatures, as k-mers can be used to quantify accurately gene expression in a less resource-consuming way. We present the Kmerator Suite, a set of three tools designed to extract specific k-mer signatures, quantify these k-mers into RNA-seq datasets and quickly visualize large dataset characteristics. The core tool, Kmerator, produces specific k-mers for 97% of human genes, enabling the measure of gene expression with high accuracy in simulated datasets. KmerExploR, a direct application of Kmerator, uses a set of predictor gene-specific k-mers to infer metadata including library protocol, sample features or contaminations from RNA-seq datasets. KmerExploR results are visualized through a user-friendly interface. Moreover, we demonstrate that the Kmerator Suite can be used for advanced queries targeting known or new biomarkers such as mutations, gene fusions or long non-coding RNAs for human health applications

MicroRNAs: new candidates for the regulation of the human cumulus–oocyte complex

International audienceWhat is the expression pattern of microRNAs (miRNAs) in human cumulus-oocyte complexes (COCs)?Not applicable