Effects of two platelet-rich gel supernatants at two concentrations on healthy cartilage explants from horses

Platelet-rich plasma is used as a treatment of arthropathies in horses. However, there is lack of in vitro information about the mechanism of action of this substance and its effects on healthy cartilage of these animals. The aims were: (1) to evaluate the effects at 48 and 96 h of two concentrations (25 and 50%) of leukocyte- and platelet-rich gel (L-PRG) and pure platelet rich gel (P-PRG) supernatants on the production/degradation in normal cartilage explants (CEs) of platelet-derived growth factor isoform BB, transforming growth factor beta-1, tumour necrosis factor alpha, interleukin (IL) 4 (IL-4), and IL-1 receptor antagonist (IL-1ra); and (2) to study possible correlations of these molecules with their respective PRG supernatant treatments. CEs from six horses were cultured for 96 h with L-PRG and P-PRG supernatants at 25 and 50% concentrations, respectively. CE culture media were changed each 48 h and used for determination, by ELISA, of the molecules. L-PRG and P-PRG supernatants at 25 and 50% concentrations influenced the molecular anti-inflammatory profile of CE groups cultured with these substances. 50% L-PRG supernatant produced the most robust pro-inflammatory effects when compared to the CE control group and the CE group treated with the other PRG supernatant concentrations. In general, PRG supernatants induced pro-inflammatory effects in normal CEs

Controle da podridão cinzenta da maçã por produtos naturais biologicamente ativos

Indexación: Web of Science; ScieloBiorend SC (chitosan), BC-1000 EC (grapefruit extract plus bioflavonoids) and ECO-100 SC (bioflavonoids plus organic acids, citric phytoalexins, fatty acids, glycerides and sugars), respectively, suppressed grey rot of apple caused by B. cinerea by 80.1%, 79.0% and 76.5% when used as post-harvest treatments under controlled conditions. When applied as combined pre- and post-harvest treatments Biorend SC inhibited fruit rot by 49.9 %, while BC-1000 EC and ECO-100 SC were ineffective. None of the products inhibited fruit rot when applied as pre-harvest treatments under controlled conditions or as post-harvest treatments under commercial conditions. The algal polysaccharide ulvan used in post-harvest treatments suppressed grey rot by 56.0% under controlled conditions, but had no inhibitory effect on combined pre- and post-harvest treatments. The inability of products to activate defense mechanisms (chitinase and peroxidase) of fruits was consistent with the unsuccessful control of rot by pre-harvest treatment. The results suggest that the natural products used have potential for use in integrated management of Botrytis rot when applied after harvest.Biorend SC (quitosana), BC-1000 EC (extrato de toranja mais flavonóides), e ECO-100 SC (bioflavonóides mais ácidos orgânicos, fitoalexinas cítricas, ácidos graxos glicerídeos e açúcares) inibiram em 80,1%, 79,0% e 76,5%, respectivamente, a podridão causada por Botrytis cinerea quando utilizados no tratamento pós-colheita de frutos de maçã sob condições controladas.Tratamento combinado de Biorend SC, com aplicação tanto em pré como no pós-colheita, proporcionou 49,9% de inibição da podridão, enquanto BC-1000 e ECO-100 EC não foram efetivos. Nenhum desses produtos inibiu a podridão cinzenta, quando utilizados em tratamento de pré-colheita em condições controladas ou em tratamento de pós-colheita em condições comerciais. O polissacarídeo algal ulvana, utilizado nos tratamentos de pós-colheita, reduziu em 56% a podridão cinzenta das maçãs em condições controladas, mas não teve efeito inibitório nos tratamentos combinados de pré e pós-colheita. A incapacidade dos produtos em ativar mecanismos de defesa (quitinases e peroxidases) nos frutos, após o tratamento em pré-colheita, foi consistente com a falta de controle da podridão nesse tipo de ensaio. Pelos resultados, sugere-se que os produtos naturais utilizados apresentam potencial para a utilização no manejo integrado da podridão de Botrytis quando aplicados em pós-colheita.http://ref.scielo.org/kcxb9

Special Issue on Spin Statistics

A workshop dedicated to spin statistics—"SpinStat2008"—was held at the end of October 2008 at the Stazione Marittima Conference Center in Trieste, Italy: it was meant to focus especially on experimental and theoretical aspects of the spin-statistics connection and of related symmetries (in particular the CPT and the Lorentz symmetries). The workshop was quite successful and everybody there felt that there should be a follow-up, and that the many interesting contributions and ideas presented there should be put in paper form. After some thinking we decided that the best format would be a series of refereed papers in a topical issue of an outstanding journal, open to all contributors, rather than the usual volume of conference papers restricted to participants. The Editors of Foundations of Physics kindly accepted to host this topical issue, and the ensuing call for papers drew a respectable flow of interesting and novel ideas, and some very appealing review papers. The comments that follow have bee

Increasing serum calcium levels and recent return from transplantation as clues to the tuberculous nature of refractory peritoneal dialysis peritonitis

Peritoneal tuberculosis is an uncommon complication of peritoneal dialysis in Europe. It is more common in Asian immigrants. A delayed diagnosis is frequent and impairs patient outcomes. We present two cases of peritoneal tuberculosis with common features that may help suspect the disease early countries with a low incidence. Both patients were females (of Spanish origin) who had recently restarted peritoneal dialysis following kidney transplantation. Both developed bacterial peritonitis clinically that was refractory to conventional antibiotics, despite clearance of bacteria. Both stopped calcium-containing phosphate binders because of increasing serum calcium that in one case led to frank hypercalcemia that persisted despite low calcium dialysate. Peritoneal biopsy was the first positive test in both cases. This report emphasizes the recent return from transplantation and rising serum calcium levels as features that should alert the physician of a potential underlying tuberculous peritonitisGrant support: ISCII and FEDER funds FIS PS09/00447, PI13/00047, ISCIII-RETIC REDinREN/RD12/0021/0001, Comunidad de Madrid/CIFRA/ S2010/BMD-2378. Salary support: FIS Rio Hortega to BFF, CMC, LRO, Programa Intensificación Actividad Investigadora (ISCIII/Agencia Laín- Entralgo/CM) to A

Formulación de plan de gestión integral de residuos sólidos en la empresa Grenn Waste SAS ESP

Diseñar el plan de gestión integra de residuos sólidos en la compañía Green Waste SAS ESP como herramienta técnica para garantizar el mejoramiento continuo de los procesos y de las actividades de gestión ambiental empresarial.Introducción. L En la Resolución 1045 de 2013 se establece la metodología para la formulación, implementación, evaluación, seguimiento, control y actualización de los Planes de Gestión Integral de Residuos Sólidos. A partir de esta normativa se determinan los lineamientos técnicos para ejecutar de una manera eficaz el manejo óptimo de los residuos generados en la organización. Objetivo. Diseñar el plan de gestión integra de residuos sólidos en la compañía Green Waste SAS ESP como herramienta técnica para garantizar el mejoramiento continuo de los procesos y de las actividades de gestión ambiental empresarial. Metodología. el enfoque metodológico empleado para la realización del presente proyecto corresponde a uno cualitativo, dado que desarrollara una idea que parte de un problema, el tener el documento PGIR en la empresa por normativa, esto hará que se realice una exploración a la empresa y diseño del plan al igual que el análisis de datos, adicionalmente cuenta con un enfoque inductivo por la investigación contextual, reflexión y recopilación de datos Resultados. El resultado de este proyecto es el plan de gestión integral de residuos sólidos para la empresa Green Waste SAS ESP

Investigar en tiempos de aislamiento social. Decisiones metodológicas en una investigación en curso

En el artículo se reflexiona sobre el alcance epistemológico de la incorporación de soportes tecnológicos para la recolección de datos y realización de entrevistas en profundidad en una investigación de tesis doctoral en curso afectada en su continuidad por la pandemia de COVID-19. Se pone a disposición el análisis sobre las posibilidades y límites de los medios digitales y nuevas tecnologías para garantizar la integridad de los datos al momento de incorporar modificaciones en el proceso de recolección de información y se explicitan las decisiones asumidas en torno a las herramientas y modalidad de recolección de datos para continuar con la investigación doctoral. Palabras clave: investigación cualitativa, investigación electrónica, investigación en pandemia, entornos virtuales, técnicas de recolección de datos