Trematode Centrocestus formosanus Infection and Distribution in Ornamental Fishes in Mexico

Análisis de la distribución del Trematodo Centrocestus formosanus en peces de ornatoThe aim of this study was to determine the ornamental fish species affected by the metacercariae of the digenean trematode Centrocestus formosanus and its distribution in 48 fish farms in Morelos, central Mexico. The parasite was found to form various numbers of branchial cysts in 11 of the 25 species analyzed. Goldfish Carassius auratus was the most commonly affected species; 20 of 30 farms were positive for this parasite, the fish showing severe clinical signs and having a high mortality rate. For the first time in the region, koi (a variant of common carp Cyprinus carpio), zebrafish Danio rerio, suckermouth catfish Hypostomus plecostomus and blue gourami Trichogaster trichopterus (also known as the threespot gourami) were positive; meanwhile, Mexican tetra Astyanax mexicanus, which had previously been reported positive in other studies in this region, was found to be negative. The parasite was observed in fish from 27 of the 48 farms studied. Ten of 15 municipalities had farms that were positive for the parasite. These results suggest that the distribution of C. formosanus is closely related to the use of water that had been contaminated with the parasite and to the presence of a snail, the red-rimmed melania Melanoides tuberculata.Asociación de Productores de Peces de Ornato of Morelos State

Perinatal outcomes in monochorionic multiple pregnancies regarding the placental characteristics

OBJETIVO: evaluar las características placentarias de pacientes con embarazos múltiples monocoriales relacionados con el resultado obstétrico en nuestro medio. MATERIAL Y MÉTODO: estudio observacional, descriptivo, prospectivo efectuado en dos instituciones de tercer nivel de atención de Bogotá, Colombia, entre el 31 julio de 2009 y el 31 de agosto de 2011. Evaluación de pacientes con embarazos monocoriales, complicaciones, tratamientos y resultados perinatales; análisis placentarios relacionados con el número y tipo de anastomosis, forma y distancia entre las inserciones de los cordones umbilicales y distribución placentaria. RESULTADOS: se incluyeron 72 embarazos monocoriales: 93.1% correspondieron a embarazos dobles y 86.1% a biamnióticos. De las placentas analizadas, 91.6% tuvieron al menos una anastomosis, específicamente arterioarterial (70.8% de los casos). Se encontraron complicaciones propias de los embarazos monocoriales (41.6%), principalmente: restricción de crecimiento intrauterino selectivo (16 casos) y trasfusión feto-fetal (11 pares de gemelos). Se registró un caso de trasfusión arterial reversa y una muerte fetal (10.7%) provocada por síndrome de transfusión feto-fetal. Entre las causas de morbilidad neonatal se encontraron: taquipnea transitoria del recién nacido y enfermedad de membrana hialina. La mortalidad neonatal fue de 5.4%. No hubo casos de mortalidad materna. CONCLUSIÓN: las características placentarias determinan las complicaciones propias del embarazo monocorial, principalmente: síndrome de trasfusión feto-fetal, restricción de crecimiento intrauterino selectivo. La detección ecográfica prenatal de estas alteraciones es importante para ofrecer consejería y vigilancia gestacional programada.OBJECTIVE: To evaluate placental characteristics in monochorionic multiple pregnancies in relation to obstetric outcome in our environment. MATERIAL AND METHOD: Prospective and observational study was made; carried to 31 July 2009 and 31 August 2011 in two high complexity institutions in Bogota, Colombia. We included monochorionic pregnancies for diagnosis of complications, management and perinatal outcome, placental analyzes were evaluated with respect to the number and type of anastomosis, type and distance between umbilical cord insertions and placental sharing. RESULTS: We registrered 72 monochorionic pregnancies, twin pregnancies were 93.1% and 86.1% diamniotic. Placentas analyzed 91.6% had at least one placental anastomosis, arterio-arterial mainly in 70.8% of patients. Complications of monochorionic pregnancies in 41.6% of cases mainly selective growth restriction in 16 cases, followed by twin to twin transfusion in 11 pairs of twins were found. A case of blood transfusion reverse was presented; and a fetal mortality of 10.7% remains the leading cause twin to twin transfusion syndrome. Among the causes of neonatal morbidity transient tachypnea of the newborn, followed by hyaline membrane disease were found. The neonatal mortality rate was 5.4%. There were no maternal deaths. CONCLUSION: Placental characteristics determine the complications of monochorionic pregnancy, as the presence of twin to twin transfusion syndrome selective growth restriction and perinatal outcome. Prenatal sonographic identification of these features is important for prenatal counseling and the frequency of gestational surveillance

Estudio de caso de sindrome neurologico asociado a astrocitoma

El astrocitoma es una neoplasia primaria del sistema nervioso central que se puede presentar en los animales; de las especies domésticas se ha observado con mayor frecuencia en los perros, de éstos, la edad y la raza son factores que influyen en su presentación, en estudios anteriores han descrito que el astrocitoma es una neoplasia que se presenta con mayor frecuencia en perros de características braquicefalicas, además que su malignidad depende de la localización y del carácter invasivo que presenta. Se presenta el estudio de caso de un paciente canino Labrador, macho de 6 años de edad, con un cuadro clínico neurológico de origen compresivo y obstructivo, derivado de una neoplasia intracraneala. Se documentó el caso a través del método clínico, basado en la historia clínica y la evolución del caso, imagenología e histopatología. Lo observado a través de las placas, correspondieron a una masa tumoral en la región ventral correspondiente al diencéfalo cuya localización se refiere adyacente a la estructura hipotalámica con efecto obstructivo y compresivo compatible con un astrocitoma; al estudio anatomopatológico se observo un tumor localizado adyacente al hipotálamo del cerebro; histológicamente el tumor se identifico como un astrocitoma gemistocítico

Experiencias interdisciplinarias en alimentación y daños a la salud en las fronteras sur y norte de México

Los problemas de salud y alimentación en diferentes contextos como las fronteras sur y norte de México exige de trabajo interdisciplinar, con lenguajes y bases comunes para los grupos académicos. El objetivo de esta ponencia es reflexionar sobre la experiencia del grupo Seminario de “Alimentación y Daños a la Salud” (ADAS), el cual busca consolidar una propuesta de colaboración interinstitucional para el abordaje interdisciplinario de los problemas de salud relacionados con la alimentación y vulnerabilidad social en la frontera sur y norte de México.Facultad de Humanidades y Ciencias de la Educació

Experiencias interdisciplinarias en alimentación y daños a la salud en las fronteras sur y norte de México

Los problemas de salud y alimentación en diferentes contextos como las fronteras sur y norte de México exige de trabajo interdisciplinar, con lenguajes y bases comunes para los grupos académicos. El objetivo de esta ponencia es reflexionar sobre la experiencia del grupo Seminario de “Alimentación y Daños a la Salud” (ADAS), el cual busca consolidar una propuesta de colaboración interinstitucional para el abordaje interdisciplinario de los problemas de salud relacionados con la alimentación y vulnerabilidad social en la frontera sur y norte de México.Facultad de Humanidades y Ciencias de la Educació

Revista de Vertebrados de la Estación Biológica de Doñana

The Osteology of Barbus bocagei (Steindachner, 1866) (Pisces: Cyprinidae).El crecimiento del Cacho (Leuciscus cephalus pyraenaicus Günther, 1862) en el rio Jarama (cuenca del Tajo)Contribución al estudio de la bermejuela, Rutilus arcasi, Steindachner 1866 de la cuenca del Júcar (Osteichthyes: Cyprinidae) 1. MorfometríaDimensiones, estructura, forma y disposición en la cavidad de los nidos de Corvus monedula.Variaciones altitudinales en la composición de las comunidades nidificantes de aves de Sierra Nevada (Sur de España)Ecomorfología de una comunidadde Passeriformes en la Sierra de Cazorla, SE de EspañaLa Distribución del pez-sol (Lepolllis gibbostlS L.) en la Península IbéricaSobre la distribución de Barbus haasi (OSTARIOPHYSI: CYPRINIDAE)La Distribución de Barbus bocagei STEINDACHNER, 1865 (OSTARIOPHYSI: CYPRINIDAE) en la Península IbéricaLighy effects on circadian locomotor activity of Lacerta lepida under constant temperatureEvaluación de diferentes métodos para estimar el área de campeo de dos especies de iguánidos.Un Casal de Cartaxo-Nottenho (Saxicola rubetra) a criar em Portugal.Nidificación de Passer hispaniolensis en antiguos nidos de Dendrocopos major thanneri.Observación de un lince ibérico (Lynx pardina) en la provincia de Lugo. Norte de España.El Visón americano, Mustela vison SCHREBER, 1777 (MAMMALIA, MUSTELIDAE) en Cataluña, N.E. de la Península Ibérica.Peer reviewe

Whither Magnetic Hyperthermia? A Tentative Roadmap

The scientific community has made great efforts in advancing magnetic hyperthermia for the last two decades after going through a sizeable research lapse from its establishment. All the progress made in various topics ranging from nanoparticle synthesis to biocompatibilization and in vivo testing have been seeking to push the forefront towards some new clinical trials. As many, they did not go at the expected pace. Today, fruitful international cooperation and the wisdom gain after a careful analysis of the lessons learned from seminal clinical trials allow us to have a future with better guarantees for a more definitive takeoff of this genuine nanotherapy against cancer. Deliberately giving prominence to a number of critical aspects, this opinion review offers a blend of state-of-the-art hints and glimpses into the future of the therapy, considering the expected evolution of science and technology behind magnetic hyperthermia

Whither magnetic hyperthermia? A tentative roadmap

The scientific community has made great efforts in advancing magnetic hyperthermia for the last two decades after going through a sizeable research lapse from its establishment. All the progress made in various topics ranging from nanoparticle synthesis to biocompatibilization and in vivo testing have been seeking to push the forefront towards some new clinical trials. As many, they did not go at the expected pace. Today, fruitful international cooperation and the wisdom gain after a careful analysis of the lessons learned from seminal clinical trials allow us to have a future with better guarantees for a more definitive takeoff of this genuine nanotherapy against cancer. Deliberately giving prominence to a number of critical aspects, this opinion review offers a blend of state-of-the-art hints and glimpses into the future of the therapy, considering the expected evolution of science and technology behind magnetic hyperthermia.status: publishe

Assessment of plasma chitotriosidase activity, CCL18/PARC concentration and NP-C suspicion index in the diagnosis of Niemann-Pick disease type C: A prospective observational study

Background: Niemann-Pick disease type C (NP-C) is a rare, autosomal recessive neurodegenerative disease caused by mutations in either the NPC1 or NPC2 genes. The diagnosis of NP-C remains challenging due to the non-specific, heterogeneous nature of signs/symptoms. This study assessed the utility of plasma chitotriosidase (ChT) and Chemokine (C-C motif) ligand 18 (CCL18)/pulmonary and activation-regulated chemokine (PARC) in conjunction with the NP-C suspicion index (NP-C SI) for guiding confirmatory laboratory testing in patients with suspected NP-C. Methods: In a prospective observational cohort study, incorporating a retrospective determination of NP-C SI scores, two different diagnostic approaches were applied in two separate groups of unrelated patients from 51 Spanish medical centers (n = 118 in both groups). From Jan 2010 to Apr 2012 (Period 1), patients with =2 clinical signs/symptoms of NP-C were considered ''suspected NP-C'' cases, and NPC1/NPC2 sequencing, plasma chitotriosidase (ChT), CCL18/PARC and sphingomyelinase levels were assessed. Based on findings in Period 1, plasma ChT and CCL18/PARC, and NP-C SI prediction scores were determined in a second group of patients between May 2012 and Apr 2014 (Period 2), and NPC1 and NPC2 were sequenced only in those with elevated ChT and/or elevated CCL18/PARC and/or NP-C SI =70. Filipin staining and 7-ketocholesterol (7-KC) measurements were performed in all patients with NP-C gene mutations, where possible. Results: In total across Periods 1 and 2, 10/236 (4%) patients had a confirmed diagnosis o NP-C based on gene sequencing (5/118 4.2%] in each Period): all of these patients had two causal NPC1 mutations. Single mutant NPC1 alleles were detected in 8/236 (3%) patients, overall. Positive filipin staining results comprised three classical and five variant biochemical phenotypes. No NPC2 mutations were detected. All patients with NPC1 mutations had high ChT activity, high CCL18/PARC concentrations and/or NP-C SI scores =70. Plasma 7-KC was higher than control cut-off values in all patients with two NPC1 mutations, and in the majority of patients with single mutations. Family studies identified three further NP-C patients. Conclusion: This approach may be very useful for laboratories that do not have mass spectrometry facilities and therefore, they cannot use other NP-C biomarkers for diagnosis

Deleterious ABCA7 mutations and transcript rescue mechanisms in early onset Alzheimer’s disease

Abstract: Premature termination codon (PTC) mutations in the ATP-Binding Cassette, Sub-Family A, Member 7 gene (ABCA7) have recently been identified as intermediate-to-high penetrant risk factor for late-onset Alzheimer's disease (LOAD). High variability, however, is observed in downstream ABCA7 mRNA and protein expression, disease penetrance, and onset age, indicative of unknown modifying factors. Here, we investigated the prevalence and disease penetrance of ABCA7 PTC mutations in a large early onset AD (EOAD)-control cohort, and examined the effect on transcript level with comprehensive third-generation long-read sequencing. We characterized the ABCA7 coding sequence with next-generation sequencing in 928 EOAD patients and 980 matched control individuals. With MetaSKAT rare variant association analysis, we observed a fivefold enrichment (p = 0.0004) of PTC mutations in EOAD patients (3%) versus controls (0.6%). Ten novel PTC mutations were only observed in patients, and PTC mutation carriers in general had an increased familial AD load. In addition, we observed nominal risk reducing trends for three common coding variants. Seven PTC mutations were further analyzed using targeted long-read cDNA sequencing on an Oxford Nanopore MinION platform. PTC-containing transcripts for each investigated PTC mutation were observed at varying proportion (5-41% of the total read count), implying incomplete nonsense-mediated mRNA decay (NMD). Furthermore, we distinguished and phased several previously unknown alternative splicing events (up to 30% of transcripts). In conjunction with PTC mutations, several of these novel ABCA7 isoforms have the potential to rescue deleterious PTC effects. In conclusion, ABCA7 PTC mutations play a substantial role in EOAD, warranting genetic screening of ABCA7 in genetically unexplained patients. Long-read cDNA sequencing revealed both varying degrees of NMD and transcript-modifying events, which may influence ABCA7 dosage, disease severity, and may create opportunities for therapeutic interventions in AD