Measurement of the threshold sensitivity of honeybees to weak, extremely low-frequency magnetic fields

Experiments reported previously demonstrate that free-flying honeybees are able to detect static intensity fluctuations as weak as 26 nT against the background, earth-strength magnetic field. We report here an extension of this work to weak, alternating fields at frequencies of 10 and 60 Hz. Our results indicate that the sensitivity of the honeybee magnetoreception system decreases rapidly with increasing frequency. At 60 Hz, alternating field strengths above 100 µT are required to elicit discrimination. These results are consistent with biophysical predictions of a magnetite-based magnetoreceptor

The z<=0.1 Surface Brightness Distribution

The surface brightness distribution (SBD) function describes the number density of galaxies as measured against their central surface brightness. Because detecting galaxies with low central surface brightnesses is both time-consuming and complicated, determining the shape of this distribution function can be difficult. In a recent paper Cross, et al. suggested a bell-shaped SBD disk-galaxy function which peaks near the canonical Freeman value of 21.7 and then falls off significantly by 23.5 B mag arcsec-2. This is in contradiction to previous studies which have typically found flat (slope=0) SBD functions out to 24 - 25 B mag arcsec^-2 (the survey limits). Here we take advantage of a recent surface-brightness limited survey by Andreon & Cuillandre which reaches considerably fainter magnitudes than the Cross, et.al sample (M_B reaches fainter than -12 for Andreon & Cuillandre while the Cross, et.al sample is limited to M_B < -16) to re-evaluate both the SBD function as found by their data and the SBD for a wide variety of galaxy surveys, including the Cross, et al. data. The result is a SBD function with a flat slope out through the survey limits of 24.5 B mag arcsec^-2, with high confidence limits.Comment: 5 pages including 5 figures. accepted by A&A

Mechanics of Graded Wrinkling

The properties and behavior of a surface as well as its interaction with surrounding media depend on the inherent material constituency and the surface topography. Structured surface topography can be achieved via surface wrinkling. Through the buckling of a thin film of stiff material bonded to a substrate of a softer material, wrinkled patterns can be created by inducing compressive stress states in the thin film. Using this same principle, we show the ability to create wrinkled topologies consisting of a highly structured gradient in amplitude and wavelength, and one which can be actively tuned. The mechanics of graded wrinkling are revealed through analytical modeling and finite element analysis, and further demonstrated with experiments.Center for Clean Water and Clean Energy at MIT and KFUP

The cryogenic system for the SLAC E158 experiment

E158 is a fixed target experiment at SLAC in which high energy (up to 48 GeV) polarized electrons are scattered off the unpolarized electrons in a 1.5 m long liquid hydrogen target. The total volume of liquid hydrogen in the system is 47.1. The beam can deposit as much as 700 W into the liquid hydrogen. Among the requirements for the system are: that density fluctuations in the liquid hydrogen be kept to a minimum, that the target can be moved out of the beam line while cold and replaced to within 2 mm and that the target survive lifetime radiation doses of up to 1×106 Gy. The cryogenic system for the experiment consists of the target itself, the cryostat containing the target, a refurbished CTI 4000 refrigerator providing more than 1 kW of cooling at 20 K and associated transfer lines and valve boxes. This paper discusses the requirements, design, construction, testing and operation of the cryogenic system. The unique features of the design associated with hydrogen safety and the high radiation field in which the target resides are also covered

Biobehavioral effects of Tai Chi Qigong in men with prostate cancer: Study design of a three-arm randomized clinical trial.

Fatigue is often one of the most commonly reported symptoms in prostate cancer survivors, but it is also one of the least understood cancer-related symptoms. Fatigue is associated with psychological distress, disruptions in sleep quality, and impairments in health-related quality of life. Moreover, inflammatory processes and changes related to the hypothalamic-pituitary-adrenal (HPA) axis and/or autonomic nervous system may also play a role in cancer-related fatigue. Thus, effective treatments for fatigue in prostate cancer survivors represent a current unmet need. Prior research has shown that Tai Chi Qigong, a mind-body exercise intervention, can improve physical and emotional health. Herein, we describe the protocol of the ongoing 3-arm randomized controlled Health Empowerment &amp; Recovery Outcomes (HERO) clincal trial. One hundred sixty-six prostate cancer survivors with fatigue are randomized to a modified Tai Chi Qigong intervention (TCQ), intensity-matched body training intervention (BT), or usual care (UC) condition. Guided by biopsychosocial and psychoneuroimmunology models, we propose that TCQ, as compared to BT or UC will: i) reduce fatigue (primary outcome) in prostate cancer survivors; ii) reduce inflammation; and iii) regulate the expression of genes from two major functional clusters: a) inflammation, vasodilation and metabolite sensing and b) energy and adrenergic activation. Assessments are conducted at baseline, the 6-week midpoint of the intervention, and 1 week, 3 months, and 12 months post-intervention. If our findings show that TCQ promotes recovery from prostate cancer and its treatment, this type of intervention can be integrated into survivorship care plans as the standard of care. The study's findings will also provide novel information about underlying biobehavioral mechanisms of cancer-related fatigue. Trial registration number:NCT03326713; clinicaltrials.gov

Mixing of magmatic-hydrothermal and metamorphic fluids and the origin of peribatholitic Sn vein-type deposits in Rwanda

The fluid sources of granite-related Sn-quartz vein deposits are commonly obscured by fluid mixing or fluid-rock interactions. As a result, fluid inclusions, minerals and isotopes in these veins indicate an intermediate composition between magmatic and metamorphic, but the degree of mixing between these endmembers is currently unquantified. This study presents a novel quantitative approach to assess the degree of mixing between magmatic-hydrothermal and external metamorphic fluids in the formation of peribatholitic Sn-quartz veins. In particular, fluid mixing in the Sn-mineralized Rwamagana-Musha-Ntunga pegmatite-quartz vein field in East Rwanda has been evaluated by the following four methods: quartz stable isotopes, muscovite geochemistry, fluid inclusion microthermometry and LA-ICP-MS, and geochemical modelling. The quartz stable isotope data (δ18O: +13.1 to +15.8 ‰ V-SMOW; δD: −27.6 to –59.7 ‰ V-SMOW) cannot uniquely differentiate between a metamorphic fluid origin or an initial magmatic hydrothermal fluid origin with subsequent metamorphic fluid mixing or host-rock interaction. However, granitophile element concentrations in magmatic muscovite from pegmatites and hydrothermal muscovite from associated Sn-quartz veins are equally high, indicating a close genetic link (Rb: 530 – 8740 ppm, Li: 110 – 1990 ppm, Sn: 87 – 810 ppm, Cs: 62 – 420 ppm). Primary H2O-CO2-N2-NaCl medium saline magmatic fluid inclusions in quartz of pegmatites (∼12.7 wt% NaCleq) and H2O-CO2-(N2)-NaCl low saline fluid inclusions in barren metamorphic quartz veins (∼4.9 wt% NaCleq) were analyzed by LA-ICP-MS. These results show an enrichment in Li, Rb, Sn and Cs for the magmatic fluid, while the metamorphic fluid is characterized by low granitophile element concentrations and high Sr and Ba contents. The expected Rb-Cs and Rb-Sn signature of the Sn-quartz vein muscovite was modelled using the measured fluid endmember compositions, confirming mixing between magmatic and metamorphic fluids in the formation of the veins. The quantification suggests that the hydrothermal Sn-quartz vein fluid contains 5 – 80 % of an external metamorphic fluid component

Multigene expression of protein complexes by iterative modification of genomic Bacmid DNA

<p>Abstract</p> <p>Background</p> <p>Many cellular multi-protein complexes are naturally present in cells at low abundance. Baculovirus expression offers one approach to produce milligram quantities of correctly folded and processed eukaryotic protein complexes. However, current strategies suffer from the need to produce large transfer vectors, and the use of repeated promoter sequences in baculovirus, which itself produces proteins that promote homologous recombination. One possible solution to these problems is to construct baculovirus genomes that express each protein in a complex from a separate locus within the viral DNA. However current methods for selecting such recombinant genomes are too inefficient to routinely modify the virus in this way.</p> <p>Results</p> <p>This paper reports a method which combines the lambda red and bacteriophage P1 Cre-recombinase systems to efficiently generate baculoviruses in which protein complexes are expressed from multiple, single-locus insertions of foreign genes. This method is based on an 88 fold improvement in the selection of recombinant viruses generated by red recombination techniques through use of a bipartite selection cassette. Using this system, seven new genetic loci were identified in the AcMNPV genome suitable for the high level expression of recombinant proteins. These loci were used to allow the recovery two recombinant virus-like particles with potential biotechnological applications (influenza A virus HA/M1 particles and bluetongue virus VP2/VP3/VP5/VP7 particles) and the mammalian chaperone and cancer drug target CCT (16 subunits formed from 8 proteins).</p> <p>Conclusion</p> <p><b>1</b>. Use of bipartite selections can significantly improve selection of modified bacterial artificial chromosomes carrying baculovirus DNA. Furthermore this approach is sufficiently robust to allow routine modification of the virus genome. <b>2</b>. In addition to the commonly used <it>p10 </it>and polyhedrin loci, the <it>ctx, egt, 39k, orf51, gp37, iap2 </it>and <it>odv-e56 </it>loci in AcMNPV are all suitable for the high level expression of heterologous genes. <b>3</b>. Two protein, four protein and eight protein complexes including virus-like particles and cellular chaperone complexes can be produced using the new approach.</p