Real-time backstepping control for fuel cell vehicle using supercapacitors

A key issue of real-time applications is ensuring the operation by taking into account the stability constraints. For multisource vehicles, stability is impacted by the multisource interactions. Backstepping control ensures stable control for most classes of nonlinear systems. Nevertheless, no backstepping control in real time has been yet proposed for multisource vehicles. The objective of this paper is to apply the backstepping control to a multisource vehicle with fuel cell and supercapacitors for real-time implementation. A distribution criterion is used to allocate energy between sources. Experimental results demonstrate that the developed backstepping control can be implemented in real-time conditions. The supercapacitors can thus help the fuel cell to meet the requirements of the load with a guarantee of system stability. © 1967-2012 IEEE

Mineralization of chitosan membrane using a double diffusion system for bone related applications

Chitosan membranes were subjected to a pre-treatment in a double diffusion system, with a calcium solution in one chamber and a phosphate solution in the other chamber. Both chambers were separated by the chitosan membrane and subject to three mineralization periods (5, 10 and 15 minutes). After this pre-treatment the bioactivity of the different calcium phosphate coatings formed was tested for different periods of immersion time, 7, 14 and 21 days at room temperature and 37ºC, in acellular simulated body fluid (1.0x). The results obtained demonstrated that the calcium phosphate coatings formed during the pre-treatment process are bioactive. It was found that the calcification is effective just in the side of the membrane exposed to the calcium solution chamber. This enabled to develop membranes with asymmetric osteoinductive properties that can be useful in different orthopedic applications

An Analysis of the Shapes of Interstellar Extinction Curves. V. The IR-Through-UV Curve Morphology

We study the IR-through-UV interstellar extinction curves towards 328 Galactic B and late-O stars. We use a new technique which employs stellar atmosphere models in lieu of unreddened "standard" stars. This technique is capable of virtually eliminating spectral mismatch errors in the curves. It also allows a quantitative assessment of the errors and enables a rigorous testing of the significance of relationships between various curve parameters, regardless of whether their uncertainties are correlated. Analysis of the curves gives the following results: (1) In accord with our previous findings, the central position of the 2175 A extinction bump is mildly variable, its width is highly variable, and the two variations are unrelated. (2) Strong correlations are found among some extinction properties within the UV region, and within the IR region. (3) With the exception of a few curves with extreme (i.e., large) values of R(V), the UV and IR portions of Galactic extinction curves are not correlated with each other. (4) The large sightline-to-sightline variation seen in our sample implies that any average Galactic extinction curve will always reflect the biases of its parent sample. (5) The use of an average curve to deredden a spectral energy distribution (SED) will result in significant errors, and a realistic error budget for the dereddened SED must include the observed variance of Galactic curves. While the observed large sightline-to-sightline variations, and the lack of correlation among the various features of the curves, make it difficult to meaningfully characterize average extinction properties, they demonstrate that extinction curves respond sensitively to local conditions. Thus, each curve contains potentially unique information about the grains along its sightline.Comment: To appear in the Astrophysical Journal, Part 1, July 1, 2007. Figures and Tables which will appear only in the electronic version of the Journal can be obtained via anonymous ftp from ftp://ftp.astronomy.villanova.edu . After logging in, change directories to "fitz/FMV_EXTINCTION". A README file describes the various files present in the director

Same Not the Same: Thermally Driven Transformation of Nickel Phosphinate-Bipyridine One-Dimensional Chains into Three-Dimensional Coordination Polymers

Three one-dimensional nickel coordination polymers (CPs) based on P,P′-diphenylethylenediphosphinic acid and three different bis-pyridine coligands, namely, 4,4′-bipyridine (bipy), 1,2-bis(4-pyridyl)ethane (bpy-ane), and 1,2-bis(4-pyridyl)ethylene (bpy-ene), were prepared in mild hydrothermal conditions from water solutions containing the dissolved reagents. The CPs have the formula [Ni(H2O)4(bipy)·pc2p]n (1), [Ni(H2O)4(bpy-ane)·pc2p]n (2), and [Ni(H2O)4(bpy-ene)·pc2p]n (3), and their structural features were investigated by single crystal X-ray diffraction, UV–vis, Fourier transform infrared spectroscopies, and magnetic measurements. They are constituted of infinite Ni(H2O)4(bis-pyridine) one-dimensional rows connected, through hydrogen bonds, with the phosphinic acids placed among adjacent rows. Although the formulas and the structural topologies of the three compounds are almost identical, they behave in different manners upon heating. Compound 1 yields an amorphous phase when water molecules are thermally removed, whereas compound 3 undergoes interesting phase transformations derived from the connection of Ni atoms with the phosphinates oxygen atoms, increasing the dimensionality to three-dimensional and maintaining crystallinity. The behavior of compound 2 has some analogies to that of 3, although a complete structural characterization was not performed because of a significant crystallinity loss of the heated phase. The structural features were studied by means of a combination of variable temperature single crystal and powder X-ray diffraction and thermogravimetric analysis. The reason for these different behaviors was ascribed to both the length and the flexibility degree of the nitrogenated coligands

An ecological correction to marine reserves boundaries in the US Virgin Islands

Marine protected areas (MPAs) are important tools for management of marine ecosystems. While desired, ecological and biological criteria are not always feasible to consider when establishing protected areas. In 2001, the Virgin Islands Coral Reef National Monument (VICR) in St. John, US Virgin Islands was established by Executive Order. VICR boundaries were based on administrative determination of Territorial Sea boundaries and land ownership at the time of the Territorial Submerged Lands Act of 1974. VICR prohibits almost all fishing and other extractive uses. Surveys of habitat and fishes inside and outside of VICR were conducted in 2002-07. Based on these surveys, areas outside VICR had significantly more hard corals; greater habitat complexity; and greater richness, abundance and biomass of reef fishes than areas within VICR, further supporting results from 2002-2004 (Monaco et al., 2007). The administrative (political) process used to establish VICR did not allow a robust ecological characterization of the area to determine the boundaries of the MPA. Efforts are underway to increase amounts of complex reef habitat within VICR by swapping a part of VICR that has little coral reef habitat for a Territorially-owned area within VICR that contains a coral reef with higher coral cover

Identification and characterization of a novel non-structural protein of bluetongue virus

Bluetongue virus (BTV) is the causative agent of a major disease of livestock (bluetongue). For over two decades, it has been widely accepted that the 10 segments of the dsRNA genome of BTV encode for 7 structural and 3 non-structural proteins. The non-structural proteins (NS1, NS2, NS3/NS3a) play different key roles during the viral replication cycle. In this study we show that BTV expresses a fourth non-structural protein (that we designated NS4) encoded by an open reading frame in segment 9 overlapping the open reading frame encoding VP6. NS4 is 77–79 amino acid residues in length and highly conserved among several BTV serotypes/strains. NS4 was expressed early post-infection and localized in the nucleoli of BTV infected cells. By reverse genetics, we showed that NS4 is dispensable for BTV replication in vitro, both in mammalian and insect cells, and does not affect viral virulence in murine models of bluetongue infection. Interestingly, NS4 conferred a replication advantage to BTV-8, but not to BTV-1, in cells in an interferon (IFN)-induced antiviral state. However, the BTV-1 NS4 conferred a replication advantage both to a BTV-8 reassortant containing the entire segment 9 of BTV-1 and to a BTV-8 mutant with the NS4 identical to the homologous BTV-1 protein. Collectively, this study suggests that NS4 plays an important role in virus-host interaction and is one of the mechanisms played, at least by BTV-8, to counteract the antiviral response of the host. In addition, the distinct nucleolar localization of NS4, being expressed by a virus that replicates exclusively in the cytoplasm, offers new avenues to investigate the multiple roles played by the nucleolus in the biology of the cell

Enhanced snoMEN Vectors Facilitate Establishment of GFP–HIF-1α Protein Replacement Human Cell Lines

The snoMEN (snoRNA Modulator of gene ExpressioN) vector technology was developed from a human box C/D snoRNA, HBII-180C, which contains an internal sequence that can be manipulated to make it complementary to RNA targets, allowing knock-down of targeted genes. Here we have screened additional human nucleolar snoRNAs and assessed their application for gene specific knock-downs to improve the efficiency of snoMEN vectors. We identify and characterise a new snoMEN vector, termed 47snoMEN, that is derived from box C/D snoRNA U47, demonstrating its use for knock-down of both endogenous cellular proteins and G/YFP-fusion proteins. Using multiplex 47snoMEM vectors that co-express multiple 47snoMEN in a single transcript, each of which can target different sites in the same mRNA, we document >3-fold increase in knock-down efficiency when compared with the original HBII-180C based snoMEN. The multiplex 47snoMEM vector allowed the construction of human protein replacement cell lines with improved efficiency, including the establishment of novel GFP–HIF-1α replacement cells. Quantitative mass spectrometry analysis confirmed the enhanced efficiency and specificity of protein replacement using the 47snoMEN-PR vectors. The 47snoMEN vectors expand the potential applications for snoMEN technology in gene expression studies, target validation and gene therapy

Primary versus secondary contributions to particle number concentrations in the European boundary layer

It is important to understand the relative contribution of primary and secondary particles to regional and global aerosol so that models can attribute aerosol radiative forcing to different sources. In large-scale models, there is considerable uncertainty associated with treatments of particle formation (nucleation) in the boundary layer (BL) and in the size distribution of emitted primary particles, leading to uncertainties in predicted cloud condensation nuclei (CCN) concentrations. Here we quantify how primary particle emissions and secondary particle formation influence size-resolved particle number concentrations in the BL using a global aerosol microphysics model and aircraft and ground site observations made during the May 2008 campaign of the European Integrated Project on Aerosol Cloud Climate Air Quality Interactions (EUCAARI). We tested four different parameterisations for BL nucleation and two assumptions for the emission size distribution of anthropogenic and wildfire carbonaceous particles. When we emit carbonaceous particles at small sizes (as recommended by the Aerosol Intercomparison project, AEROCOM), the spatial distributions of campaign-mean number concentrations of particles with diameter >50 nm (N50) and >100 nm (N100) were well captured by the model (R2≥0.8) and the normalised mean bias (NMB) was also small (−18% for N50 and −1% for N100). Emission of carbonaceous particles at larger sizes, which we consider to be more realistic for low spatial resolution global models, results in equally good correlation but larger bias (R2≥0.8, NMB = −52% and −29%), which could be partly but not entirely compensated by BL nucleation. Within the uncertainty of the observations and accounting for the uncertainty in the size of emitted primary particles, BL nucleation makes a statistically significant contribution to CCN-sized particles at less than a quarter of the ground sites. Our results show that a major source of uncertainty in CCN-sized particles in polluted European air is the emitted size of primary carbonaceous particles. New information is required not just from direct observations, but also to determine the "effective emission size" and composition of primary particles appropriate for different resolution models

Nucleolar protein CSIG is required for p33ING1 function in UV-induced apoptosis

Cellular senescence-inhibited gene (CSIG) protein, a nucleolar protein with a ribosomal L1 domain in its N-terminus, can exert non-ribosomal functions to regulate biological processes, such as cellular senescence. Here, we describe a previously unknown function for CSIG: promotion of apoptosis in response to ultraviolet (UV) irradiation-induced CSIG upregulation. We identified p33ING1 as a binding partner that interacts with CSIG. After UV irradiation, p33ING1 increases its protein expression, translocates into the nucleolus and binds CSIG. p33ING1 requires its nucleolar targeting sequence region to interact with CSIG and enhance CSIG protein stability, which is essential for activation of downstream effectors, Bcl-2-associated X protein, to promote apoptosis. Thus, our data imply that p33ING1–CSIG axis functions as a novel pro-apoptotic regulator in response to DNA damage