UV-induced fragmentation of Cajal bodies

The morphology and composition of subnuclear organelles, such as Cajal bodies (CBs), nucleoli, and other nuclear bodies, is dynamic and can change in response to a variety of cell stimuli, including stress. We show that UV-C irradiation disrupts CBs and alters the distribution of a specific subset of CB components. The effect of UV-C on CBs differs from previously reported effects of transcription inhibitors. We demonstrate that the mechanism underlying the response of CBs to UV-C is mediated, at least in part, by PA28γ (proteasome activator subunit γ). The presence of PA28γ in coilin-containing complexes is increased by UV-C. Overexpression of PA28γ, in the absence of UV-C treatment, provokes a similar redistribution of the same subset of CB components that respond to UV-C. RNA interference–mediated knockdown of PA28γ attenuates the nuclear disruption caused by UV-C. These data demonstrate that CBs are specific nuclear targets of cellular stress-response pathways and identify PA28γ as a novel regulator of CB integrity

Hsp90 picks PIKKs via R2TP and Tel2

Phosphatidylinositol-3 kinase-like kinases (PIKKs) are dependent on Hsp90 for their activation via the R2TP complex and Tel2. In this issue of Structure, Pal and colleagues present the molecular mechanism by which PIKKs are recruited to Hsp90

Characterization and modeling of a hybrid electric vehicle lithium-ion battery pack at low temperatures

Although lithium-ion batteries have penetrated hybrid electric vehicles (HEVs) and pure electric vehicles (EVs), they suffer from significant power capability losses and reduced energy at low temperatures. To evaluate those losses and to make an efficient design, good models are required for system simulation. Subzero battery operation involves nonclassical thermal behavior. Consequently, simple electrical models are not sufficient to predict bad performance or damage to systems involving batteries at subzero temperatures. This paper presents the development of an electrical and thermal model of an HEV lithium-ion battery pack. This model has been developed with MATLAB/Simulink to investigate the output characteristics of lithium-ion batteries over the selected operating range of currents and battery capacities. In addition, a thermal modeling method has been developed for this model so that it can predict the battery core and crust temperature by including the effect of internal resistance. First, various discharge tests on one cell are carried out, and then, cell's parameters and thermal characteristics are obtained. The single-cell model proposed is shown to be accurate by analyzing the simulation data and test results. Next, real working conditions tests are performed, and simulation calculations on one cell are presented. In the end, the simulation results of a battery pack under HEV driving cycle conditions show that the characteristics of the proposed model allow a good comparison with data from an actual lithium-ion battery pack used in an HEV. © 2015 IEEE

Real time adaptive efficient cold start strategy for proton exchange membrane fuel cells

Cold start of proton exchange membrane fuel cells (PEMFCs) at sub-zero temperatures is perceived as one of the obstacles in their commercialization way in automotive application. This paper proposes a novel internal-based adaptive strategy for the cold start of PEMFC to control its operating current in real time in a way to maximize the generated heat flux and electrical power in a short time span. In this respect, firstly, an online parameter identification method is integrated into a semi-empirical model to cope with the PEMFC performances drifts during cold start. Subsequently, an optimization algorithm is launched to find the best operating points from the updated model. Finally, the determined operating point, which is the current corresponding to the maximum power, is applied to PEMFC to achieve a rapid cold start. It should be noted that the utilization of adaptive filters has escaped the attention of previous PEMFC cold start studies. The ultimate results of the proposed strategy are experimentally validated and compared to the most commonly used cold start strategies based on Potentiostatic and Galvanostatic modes. The experimental outcomes of the comparative study indicate the striking superior performance of the proposed strategy in terms of heating time and energy requirement. © 2018 Elsevier Lt

Investigating the impact of ageing and thermal management of a fuel cell system on energy management strategies

This paper studies the impact of two significant aspects, namely fuel cell (FC) degradation and thermal management, over the performance of an optimal and a rule-based energy management strategy (EMS) in a fuel cell hybrid electric vehicle (FCHEV). To do so, firstly, a vehicle's model is developed in simulation environment for a low-speed FCHEV composed of a FC stack and a battery pack. Subsequently, deterministic dynamic programming (DP), as an optimal strategy, and bounded load following strategy (BLFS), as a common rule-based strategy, are utilized to minimize the hydrogen consumption while respecting the operating constraints of the power sources. The performance of the EMSs is assessed at different scenarios. The first objective is to clarify the effect of FC stack degradation on the performance of the vehicle. In this regard, each EMS determines the required current from the FC stack for two FCs with different levels of degradation. The second objective is to evaluate the thermal management contribution to improving the performance of the new FC compared to the considered cases in scenario one. In this respect, each strategy deals with determining two control variables (FC current and cooling fan duty cycle). The results of this study indicate that negligence of adapting to the PEMFC health state, as the PEMFC gets aged, can increase the hydrogen consumption up to 24.8% in DP and 12.1% in BLFS. Moreover, the integration of temperature dimension into the EMS can diminish the hydrogen consumption by 4.1% and 5.3% in DP and BLFS respectively. © 2020 Elsevier Lt

Aspects de la mise en œuvre d’un projet de langue mené par l’apprenant : la place de la sensibilisation

Nous décrivons dans cette étude divers aspects de la mise en œuvre d’un projet de langue mené par l’apprenant, dans le cadre d’un enseignement intégré d’anglais pour spécialistes d’autres disciplines à l’université Lyon 1. Pour mener à bien son projet, l’apprenant doit être conduit à découvrir et analyser par lui-même les différentes formes et structures. Ceci est rendu possible par la sensibilisation construite par un dispositif de fiches guides décrivant des tâches et activités précisément adaptées à l’objectif.At the University of Lyon 1, an English course for specialists in disciplines other than English incorporated a personal project conceived by the learner, yet in keeping with the teacher’s objectives. For the learner to work effectively, he had to be helped to discover and analyse for himself the various forms and structures involved. The teacher therefore had to create clear and explicit guidelines describing the different tasks and activities to be tackled and inviting him to improve his language and communication skills

A comprehensive review of solutions and strategies for cold start of automotive proton exchange membrane fuel cells

Proton exchange membrane fuel cell (PEMFC) can be a significant eco-friendly alternative power source for vehicles. However, under subfreezing conditions, cell degradation and irreversible performance decay can occur because of ice formation and repetitive thaw/freeze cycles. These problems have limited the further commercialization of PEMFC in cold weather countries. Thus, many improvements have been made to repair the freeze protection and rapid cold startup problems in PEMFC vehicles. In this paper, a comprehensive review dedicated to engineers of the recent research progress on the PEMFC cold start problems is presented. Systems and methods for fuel cell shutdown are summarized and classified into two categories: purge solution and material to avoid freezing. Regarding the system and solutions for PEMFC cold startup, different heating solutions are classified into two main groups depending on their heating sources and categorized as internal and external heating methods. This paper concludes with a detailed review of cold startup strategies based on an exhaustive survey of journal papers and patents. © 2016 IEEE

Efficiency upgrade of hybrid fuel cell vehicles' energy management strategies by online systemic management of fuel cell

This paper puts forward an approach for boosting the efficiency of energy management strategies (EMSs) in fuel cell hybrid electric vehicles (FCHEVs) using an online systemic management of the fuel cell system (FCS). Unlike other similar works which solely determine the requested current from the FCS, this work capitalizes on simultaneous regulation of current and temperature, which have different dynamic behavior. In this regard, firstly, an online systemic management scheme is developed to guarantee the supply of the requested power from the stack with the highest efficiency. This scheme is based on an updatable 3D map which relates the requested power from the stack to its optimal temperature and current. Secondly, two different EMSs are used to distribute the power between the FCS and battery. The EMSs' constraints are constantly updated by the online model to embrace the stack performance drifts owing to degradation and operating conditions variation. Finally, the effect of integrating the developed online systemic management into the EMSs' design is experimentally scrutinized under two standard driving cycles and indicated that up to 3.7% efficiency enhancement can be reached by employing such a systemic approach. Moreover, FCS health adaptation unawareness can increase the hydrogen consumption up to 6.6%