1,686 research outputs found
Mutations in the 1,25-dihydroxyvitamin D-3 receptor identifying C-terminal amino acids required for transcriptional activation that are functionally dissociated from hormone binding, heterodimeric DNA binding, and interaction with basal transcription factor IIB, in vitro
De cómo el autor soprende el diálogo entre un hombre viejo y uno joven
Ilustran el artículo dos estambpas de Baltasar Talamantes sobre “La escala de la vida”, en masculino y en femenino, en que se representan las diferentes etapas por décadas de edad, desde el nacimiento hasta la muerte
Discurso por la Real Jurisdicion, sobre la forma, y modo que deve observarse en las execuciones de las sentencias criminales
Precede al tít. (en p.1) viñeta con la inscripción "Iesus, Maria, Iosef, y S. Bernardo"Copia digital : Diputación Provincial de Zaragoza. Servicio de Archivos y Bibliotecas, 2010Datos de tít. tomados de p.1Datos de autor tomados del final del textoFecha tomada del final del textoSign.: A-N\p2\s, O\p3\
Respuesta del memorial que se ha entregado en defensa de D. Pedro Joseph Salinas infanzon y ciudadano de la ciudad de Zaragoça. Y de Juan Pallas su cajero en la Ilustrissima Iunta de la veintena... Zaragoza mayo 4 de 1659
Precede al texto , en p.1, cruz de Malta, y debajo viñeta con la inscripción "Jesus, Maria, Joseph"Copia digital : Diputación Provincial de Zaragoza. Servicio de Archivos y Bibliotecas, 2010Datos de tít. tomados de p.1Fecha tomada del final del textoSign.: A-D\p2\s, E\p1\
Relación de los derechos declarados, y adjudicados al Real Monasterio de N. Señora de Piedra, capitulos de las iglesias parroquiales de Ibdes y Munebrega, concejos y vezinos de dichos lugares y de la villa de Carenas, por la sentencia arbitral de Somed, y tres adiciones, y declaraciones della, dadas entre las dichas partes, por ... Antonio Blanco y Gomez, y D. Bartolomè Perez de Nueros...
Reclamos y apostillas marginalesCopia digital : Diputación Provincial de Zaragoza. Servicio de Archivos y Bibliotecas, 2010Datos de tit. tomados de p.1Sin pie de imp. al final del texto aparece la fecha de 1682Sign.: A\p6\sInic. grab. xil. en p.
Impact of inhaled corticosteroids on growth in children with asthma: systematic review and meta-analysis
Background: Long-term inhaled corticosteroids (ICS) may reduce growth velocity and final height of children with asthma. We aimed to evaluate the association between ICS use of >12 months and growth. Methods: We initially searched MEDLINE and EMBASE in July 2013, followed by a PubMed search updated to December 2014. We selected RCTs and controlled observational studies of ICS use in patients with asthma. We conducted random effects meta-analysis of mean differences in growth velocity (cm/year) or final height (cm) between groups. Heterogeneity was assessed using the I2 statistic. Results: We found 23 relevant studies (twenty RCTs and three observational studies) after screening 1882 hits. Meta-analysis of 16 RCTs showed that ICS use significantly reduced growth velocity at one year follow-up (mean difference -0.48 cm/year (95% CI -0.66 to -0.29)). There was evidence of a dose-response effect in three RCTs. Final adult height showed a mean reduction of -1.20 cm (95% CI -1.90 cm to -0.50 cm) with budesonide versus placebo in a high quality RCT. Meta-analysis of two lower quality observational studies revealed uncertainty in the association between ICS use and final adult height, pooled mean difference -0.85 cm (95% CI -3.35 to 1.65). Conclusion: Use of ICS for >12 months in children with asthma has a limited impact on annual growth velocity. In ICS users, there is a slight reduction of about a centimeter in final adult height, which when interpreted in the context of average adult height in England (175 cm for men and 161 cm for women), represents a 0.7% reduction compared to non-ICS users
Applications of Direct Injection Soft Chemical Ionisation-Mass Spectrometry for the Detection of Pre-blast Smokeless Powder Organic Additives
Analysis of smokeless powders is of interest from forensics and security perspectives. This article reports the detection of smokeless powder organic additives (in their pre-detonation condition), namely the stabiliser diphenylamine and its derivatives 2-nitrodiphenylamine and 4-nitrodiphenylamine, and the additives (used both as stabilisers and plasticisers) methyl centralite and ethyl centralite, by means of swab sampling followed by thermal desorption and direct injection soft chemical ionisation-mass spectrometry. Investigations on the product ions resulting from the reactions of the reagent ions H3O+ and O2+ with additives as a function of reduced electric field are reported. The method was comprehensively evaluated in terms of linearity, sensitivity and precision. For H3O+, the limits of detection (LoD) are in the range of 41-88 pg of additive, for which the accuracy varied between 1.5 and 3.2%, precision varied between 3.7 and 7.3% and linearity showed R20.9991. For O2+, LoD are in the range of 72 to 1.4 ng, with an accuracy of between 2.8 and 4.9% and a precision between 4.5 and 8.6% and R20.9914. The validated methodology was applied to the analysis of commercial pre-blast gun powders from different manufacturers.(VLID)4826148Accepted versio
Assisted evolution enables HIV-1 to overcome a high trim5α-imposed genetic barrier to rhesus macaque tropism
Diversification of antiretroviral factors during host evolution has erected formidable barriers to cross-species retrovirus transmission. This phenomenon likely protects humans from infection by many modern retroviruses, but it has also impaired the development of primate models of HIV-1 infection. Indeed, rhesus macaques are resistant to HIV-1, in part due to restriction imposed by the TRIM5α protein (rhTRIM5α). Initially, we attempted to derive rhTRIM5α-resistant HIV-1 strains using two strategies. First, HIV-1 was passaged in engineered human cells expressing rhTRIM5α. Second, a library of randomly mutagenized capsid protein (CA) sequences was screened for mutations that reduced rhTRIM5α sensitivity. Both approaches identified several individual mutations in CA that reduced rhTRIM5α sensitivity. However, neither approach yielded mutants that were fully resistant, perhaps because the locations of the mutations suggested that TRIM5α recognizes multiple determinants on the capsid surface. Moreover, even though additive effects of various CA mutations on HIV-1 resistance to rhTRIM5α were observed, combinations that gave full resistance were highly detrimental to fitness. Therefore, we employed an 'assisted evolution' approach in which individual CA mutations that reduced rhTRIM5α sensitivity without fitness penalties were randomly assorted in a library of viral clones containing synthetic CA sequences. Subsequent passage of the viral library in rhTRIM5α-expressing cells resulted in the selection of individual viral species that were fully fit and resistant to rhTRIM5α. These viruses encoded combinations of five mutations in CA that conferred complete or near complete resistance to the disruptive effects of rhTRIM5α on incoming viral cores, by abolishing recognition of the viral capsid. Importantly, HIV-1 variants encoding these CA substitutions and SIVmac239 Vif replicated efficiently in primary rhesus macaque lymphocytes. These findings demonstrate that rhTRIM5α is difficult to but not impossible to evade, and doing so should facilitate the development of primate models of HIV-1 infection
In processu executorum Don Iosephi de Palafox por Don Felix de Rebolledo, y Palafox, cavallero noble de este Reyno
Precede al texto en p.1, viñeta con la inscripción : "Iesus, Maria, Ioseph"Copia digital : Diputación Provincial de Zaragoza. Servicio de Archivos y Bibliotecas, 2010Datos de tít. tomados de p.1Sin pie de imp. Al final del texto aparece: "Zaragoça ... 1656"Sign.: A\p4\sInic. grab. xil. en p.
All clinically-relevant blood components transmit prion disease following a single blood transfusion: a sheep model of vCJD
Variant CJD (vCJD) is an incurable, infectious human disease, likely arising from the consumption of BSE-contaminated meat products. Whilst the epidemic appears to be waning, there is much concern that vCJD infection may be perpetuated in humans by the transfusion of contaminated blood products. Since 2004, several cases of transfusion-associated vCJD transmission have been reported and linked to blood collected from pre-clinically affected donors. Using an animal model in which the disease manifested resembles that of humans affected with vCJD, we examined which blood components used in human medicine are likely to pose the greatest risk of transmitting vCJD via transfusion. We collected two full units of blood from BSE-infected donor animals during the pre-clinical phase of infection. Using methods employed by transfusion services we prepared red cell concentrates, plasma and platelets units (including leucoreduced equivalents). Following transfusion, we showed that all components contain sufficient levels of infectivity to cause disease following only a single transfusion and also that leucoreduction did not prevent disease transmission. These data suggest that all blood components are vectors for prion disease transmission, and highlight the importance of multiple control measures to minimise the risk of human to human transmission of vCJD by blood transfusion
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