Optimization of NPK levels of Clementine Sidi Aissa (Citrus reticulata Blanco) trees grafted on different citrus rootstocks

Received: August 15th, 2023 ; Accepted: October 18th, 2023 ; Published: November 5th, 2023 ; Correspondence: [email protected] present study aims to investigate the impact of various nitrogen concentrations on young Clementine Sidi Aissa citrus trees (Citrus reticulata Blanco), grafted on five citrus rootstocks namely Moroccan Carrizo citrange, French Carrizo citrange, Troyer citrange, Citrus macrophylla, and sour orange (Citrus aurantium L.). The experiment took place in greenhouses at the Experimental station of El Menzeh INRA-Morocco, with the young trees grown in containers. We applied five different nitrogen treatments (expressed as mg L-1 of N-P2O5-K2O): (0–0–0), (0–25–50), (25–25–50), (50–25–50), and (100–25–50). The split-plot experimental design was used with three replications. The findings demonstrate that the nitrogen enrichment resulted in enhanced plant growth, marked by increased plant height, rootstock and scion stem diameters, diameter and shoot length, relative water content (RWC), as well as leaf chlorophyll and proline content. Optimal growth of the Clementine Sidi Aissa trees was observed under the 100–25–50 (mg L-1 of N-P2O5-K2O) treatment. The study also found that leaf nitrogen concentration increased in line with the quantity of nitrogen added, whereas the percentages of phosphorous and potassium in the leaves decreased. The most significant growth increase across the majority of the studied parameters was noted in Clementine Sidi Aissa trees grafted on Moroccan Carrizo citrange and Troyer citrange rootstocks

Magnetization study of Ni/Ag multilayers

The magnetic properties of Ni/Ag multilayers, prepared by evaporation in ultrahigh vacuum under controlled conditions, have been systematically studied by magnetic measurements. A spin-wave theory has been used to explain the temperature dependence of the magnetization and the approximate values for the bulk exchange interaction Jb and surface exchange interaction JS for various Ni layer thicknesses have been obtained.The magnetic properties of Ni/Ag multilayers, prepared by evaporation in ultrahigh vacuum under controlled conditions, have been systematically studied by magnetic measurements. A spin-wave theory has been used to explain the temperature dependence of the magnetization and the approximate values for the bulk exchange interaction Jb and surface exchange interaction JS for various Ni layer thicknesses have been obtained

Analyzing Risk Factors for Brain Damages in a Group of Newborns with High Risk of Neurodevelopmental Sequelae: A Moroccan Study

Abstract The aim of the study is to identify risk factors for occurrence of perinatal brain injury objectified in transfontanellar ultrasound (TFU) in a group of infants with high risk of neurodevelopmental sequelae. It’s a retrospective study, conducted at the neonatal intensive care unit of the Children's Hospital of Rabat, during the period from 1 January to 31 December 2014. Were included newborns hospitalized for a medical condition with intrauterine croissance retardation (ICUR) more than 5 days and have survived. The neurological workup includes TFU made between day 2 and day 5 of life. Data analysis was performed on SPSS 18.0 software. The level of significance was p <0.05.  The percentage of newborns that developed neurological damage to the TFU was 36%. Patients with abnormalities in the TFU had a breakdown time, duration dependence of O2, duration of hospitalization and a higher frequency of nosocomial infections than patients without abnormality. The interest in the prevention of nosocomial infections is to try to shorten the duration of invasive ventilation, favoring fast relay noninvasive ventilation. Keywords: brain injury; newborns; prematurity, neurodevelopmental sequelae, trans-fontanellar ultrasound.

Effect of nitrogen level application on yield and fruit quality of Navel orange variety in a sandy soil

L’objectif de cet essai est d'évaluer l'effet de la dose d'azote sur le rendement et la qualité des fruits et de déterminer la dose optimale en azote pour les arbres de l’orange Navel greffé sur le porte-greffe citrange Troyer planté en 1964 en plein champ à un espacement de 7mx7m (204 arbres/ha) au niveau de la station expérimentale d’El Menzeh, INRA Maroc. Le dispositif adopté est un bloc aléatoire complet avec trois blocs rangés en 4 arbres consécutifs par bloc sur la même ligne. Quatre doses de N ont été testés : (T0 (Témoin) : 0, T1 : 100, T2 : 200, T3 : 300 Kg N/ha). Les paramètres mesurés sont : le calibre des fruits, le poids moyen par fruit, la qualité des fruits (le rendement en jus, l’acidité du jus, l’extrait sec soluble (ESS ou °Brix) et l’indice de maturité (E/A)) et le rendement en fruits. Le rendement en fruits, le poids moyen des fruits, le calibre des fruits, l’extrait sec soluble (TSS), l'acidité du jus et l'indice de maturité de l’orange Navel ont répondu significativement à l’augmentation de la dose d’azote. Le rendement en kg de fruits/arbre a été considérablement amélioré avec l'augmentation de la dose d'azote. Par ailleurs, les rendements les plus élevés (129.21 et 139.82 kg/arbre) ont été enregistrés sous les traitements T2 et T3 respectivement. Par contre, le rendement en fruits/arbre le plus faible a été obtenu chez les arbres non fertilisés (témoin). Le poids des fruits (268.54 et 276.50g) et la taille des fruits (78.94 et 80.87 mm) les plus élevés ont été enregistrés avec les même traitements T2 et T3 respectivement. La teneur totale en sucre soluble (TSS) la plus élevée a été enregistrée chez les arbres des traitements T2 et T3 (12.20 et 11.70 °Brix respectivement). Par ailleurs, une augmentation significative de l'acidité du jus de fruits de l’orange Navel a été également enregistrée en augmentant la dose d’azote. L'acidité la plus élevée a été obtenue par les mêmes traitements T2 et T3 (1,13 et 1.20 % respectivement). L’application de 200 Kg de N /ha a permis d’avoir un rendement maximal et une qualité optimale des fruits des oranges Navel greffés sur le porte-greffe citrange Troyer sur un sol sableux dans la région de Gharb.The objective of the present paper was to evaluate the effect of nitrogen level on yield and fruit quality of citrus and to determine the optimum N fertilizer rates of Navel orange variety budded on Troyer citrange rootstock in field conditions. The orange Navel orchard spaced 7mx7m (204 trees/ha) grown in 1964 on a sandy soil at the experimental station of El Menzeh, INRA Morocco. The experiment was set up in a complete randomized block design with three blocks and the experimental unit was composed on 4 consecutive trees in the row. Four fertilization treatments of N (Kg/ha) (T0 (control): 0, T1: 100, T2: 200, T3: 300) were applied to Navel citrus tree in field conditions over two seasons 2012-2013 and 2013-2014.Fruit yield, fruit weight, fruit size, total soluble sugar (TSS) content, acidity of juice and maturity index of Navel orange were significantly affected by increasing N level. Fruit yield/tree was improved significantly with increase in N rate. Significant highest yields (129.21 and 139.82. kg/tree) were recorded under treatments T2 and T3 respectively. The lowest fruit yield/tree was obtained from untreated control plot. Maximum fruit weight (268.54 and 276.50 g) and fruit size (78.94 and 80.87 mm) was recorded with the same treatments T2 and T3 respectively. Total soluble sugar content was also maximum in trees receiving treatment (T2) and treatment (T3) 12.20 and 11.70°Brix respectively. Significant increase in acidity of juice of fruit Navel orange was also recorded by increasing the rate of N. The highest acidity (1.13 and 1.20%) was achieved by the same treatments T2 and T3 respectively. Two-year studies showed that application of fertilizers at the rate of 200 Kg N/ha would be optimum for achieving higher yield and better fruit quality of Navel orange variety in sandy soil in Gharb area

Comparative pathogenicity of Colletotrichum spp. against different varieties of strawberry plants (Fragaria ananassa) widely grown in Morocco

The evolution of anthracnose symptoms on the aerial part (leaves, stems and strawberries) of three varieties Fortuna, Camarosa and Festival of strawberry plants inoculated with the conidial suspensions of Colletotrichum acutatum and Colletotrichum gloeosporioides isolates was followed. The severity index and infection coefficients increased in function of time. Seven days after inoculation they were low not exceeding 13.43% and 43.33, but they increased four weeks after inoculation, respectively, to 37.96% and 99 on strawberry plants of the Camarosa variety, 54.44% and 105 on those of Fortuna and 51.12% and 85 on those of Festival. At the sixth week, the severity index and infection coefficients became very high, reaching respectively 100% and 408 on Fortuna plants inoculated with C. gloeosporioides isolate (Coll3) followed by Coll2 (89.28% – 300), Coll1 (86.66% – 378) and Coll4 (80.45% – 198) of C. acutatum species. Similarly, the isolate Coll3 caused fruit rot; the percentage of rotten strawberries was 100% on Fortuna variety, 83.33% on Festival and 70.25% on Camarosa. A positive re-isolation of the tested Colletotrichum isolates has been noted from leaves of strawberry varieties and negative from crowns or the roots. A significant to moderate reduction in fresh and dry weights of the aerial part and roots was noted in inoculated strawberry plants compared to the control

Effect of plant growth promoting rhizobacterial (PGPR) inoculation on growth in tomato (Solanum Lycopersicum L.) and characterization for direct PGP abilities in Morocco

Plant Growth promoting rhizobacteria are a heterogeneous group of bacteria that can be found in the rhizosphere, at root surfaces and in association with roots. They benefit plants through Production of plant hormones, such as auxins, asymbiotic N2 fixation, solubilization of mineral phosphates, antagonism against phytopathogenic microorganisms by production of antibiotics, siderophroes, Chitinase and other nutrients ability to effectively colonize roots are responsible for plant growth promotion. An experiment was conducted in the field of National Institute of Agronomic Research of Meknes. Morocco. The experiment was a completely randomized design with six replicates. There were four treatments viz. T1: (control; N0 -PGPR), T2: (N0 +2027-2), T3: (N0 +2066-7) and T4: (N0+2025-1). The results indicated that a remarkable increase in root growth, namely length, the diameter of the rod and the total chlorophyll. A total of three different bacteria colonies were isolated and proceed with in vitro screening for plant growth promoting activities; phosphate solubilization, nitrogen fixation, indole acetic acid (IAA), ammonia production and antimicrobial enzymes (cellulose, chitinase and protease) activity. Among the three bacterial strains, all bacterial strains are able to produce ammonia, IAA production and nitrogen fixation activity, one strain phosphate solubilizing activity, two strain are able to produce cellulase syntheses, Protease activity and Chitinase activity

Rapid and sensitive methods for detection of Allorhizobium vitis, causal agent of grapevine crown gall

A rapid method and sensitive methods for extraction of bacterial DNA from pure culture and directly from plant materiel were compared in polymerase chain reaction with specific primers VCF3/VCR3 to see the reliable method that can used in the detection of tumorigenic strain of Allorhizobium vitis causal agent of grapevine crown gall. From the three tested methods of DNA extraction from pure culture, the alkaline method is the most effective technique for the extraction presenting a high sensitivity with a detection threshold equal to 5.104 CFU/ml. Five different protocols for extracting bacterial DNA from plant tissues of infected tomato, based on the use of an extraction buffer, were tested to see its usefulness in detecting pathogenic strain of A. vitisS4. Two protocols based on the use of Triton X-100 and Tween 20 were efficient for detecting A. vitis S4 directly from tomato tumors with a sensitivity of 103 CFU/ml for the both protocols. Consequently, these protocols were proposed as specific protocols for the detection of tumorigenic strain of A. vitis from symptomatic and asymptomatic plants

Pathogenicity of Helminthosporium rostrata on rice varieties widely grown in Morocco

The plants of rice varieties (Arco, Thaibonnet and Elio) were inoculated with three isolates of Helminthosporium rostrata (HR1 HR2 and HR3), isolated for the first time in Morocco from the rice seed of Taibonnet variety at the end of the growing season. The results obtained showed that all the isolates are able to induce the disease on rice plants and sporulate on the foliar lesions. HR1, HR2 and HR3 was respectively the most pathogenic on Elio (I.C = 113), Arco (I.C = 212.5), and Taibonnet (130.48). The symptoms induced by the isolates are similar to those induced by Helminthosporium sativum on rice