PISCO2: the new speckle camera of the Nice 76-cm refractor

We present the new speckle camera PISCO2 made in 2010-2012, for the 76-cm refractor of C\^ote d'Azur Observatory. It is a focal instrument dedicated to the observation of visual binary stars using high angular resolution speckle interferometry techniques to partly overcome the degradation caused by the atmospheric turbulence. Fitted with an EMCCD detector, PISCO2 allows the acquisition of short exposure images that are processed in real time by our specially designed software. Two Risley prisms are used for correcting the atmospheric dispersion. All optical settings are remotely controlled. We have already been able to observe faint, close binary stars with angular separations as small as 0".16, and visual magnitudes of about 16. We also have measured some particularly difficult systems with a magnitude difference between the two components of about 4 magnitudes. This level of performance is very promising for the detection and study of large sets of yet unknown (or partly measured) binaries with close separation and/or large magnitude difference.Comment: 13 pages, 12 figure

Mitochondrial dynamics and disease, OPA1

AbstractThe mitochondria are dynamic organelles that constantly fuse and divide. An equilibrium between fusion and fission controls the morphology of the mitochondria, which appear as dots or elongated tubules depending the prevailing force. Characterization of the components of the fission and fusion machineries has progressed considerably, and the emerging question now is what role mitochondrial dynamics play in mitochondrial and cellular functions. Its importance has been highlighted by the discovery that two human diseases are caused by mutations in the two mitochondrial pro-fusion genes, MFN2 and OPA1. This review will focus on data concerning the function of OPA1, mutations in which cause optic atrophy, with respect to the underlying pathophysiological processes

Preliminary Experiments on Unsupervised Word Discovery in Mboshi

International audienceThe necessity to document thousands of endangered languages encourages the collaboration between linguists and computer scientists in order to provide the documentary linguistics community with the support of automatic processing tools. The French-German ANR-DFG project Breaking the Unwritten Language Barrier (BULB) aims at developing such tools for three mostly unwritten African languages of the Bantu family. For one of them, Mboshi, a language originating from the " Cu-vette " region of the Republic of Congo, we investigate unsuper-vised word discovery techniques from an unsegmented stream of phonemes. We compare different models and algorithms, both monolingual and bilingual, on a new corpus in Mboshi and French, and discuss various ways to represent the data with suitable granularity. An additional French-English corpus allows us to contrast the results obtained on Mboshi and to experiment with more data

Parasite host-switching from the invasive American red-eared slider, Trachemys scripta elegans, to the native Mediterranean pond turtle, Mauremys leprosa, in natural environments

International audienceThe red-eared slider turtle, Trachemys scripta elegans, is among the most over-exploited animals and is still exported annually from the USA all over the world. Once introduced into its new environment, feral populations may arise and pose threats to local biodiversity and ecosystem functioning. In France, it is in fact considered as a risk for the Mediterranean pond turtle, Mauremys leprosa, and the European pond turtle, Emys orbicularis, as they may compete for resources and habitat. Freshwater turtles are also host to a variety of parasites including protozoans and helminths. When introduced turtles escape, parasites may spread to native species. The objective of this study was to document the extent of platyhelminth invasions from T. s. elegans to natural M. leprosa populations in northern Spain and southern France and to evaluate the risks that parasite host-switching may pose on indigenous freshwater turtle species. From DNA barcoding analysis based on the sequencing of the Cytochrome c Oxidase I gene, the Bayesian tree and p-distance comparisons of closely related haplotypes revealed a greater polystome richness within M. leprosa than expected, suggesting that host switching may take place in natural environments. Because these parasites most typically infest American turtles like Chrysemys picta marginata and Graptemys pseudogeographica in their natural home range and because parasites were also found within T. s. elegans feral populations, it is suggested that the red-eared slider would serve as a carrier for a variety of not strictly host-specific polystomes that are transmitted to M. leprosa throughout the south of France. The global trade in freshwater turtles thus provides opportunity for parasites to be transported to new destinations which could impact the physiology, behavior and survival of native turtle species

Innovative technologies for under-resourced language documentation: The BULB Project

International audienceThe project Breaking the Unwritten Language Barrier (BULB), which brings together linguists and computer scientists, aims at supporting linguists in documenting unwritten languages. In order to achieve this we will develop tools tailored to the needs of documentary linguists by building upon technology and expertise from the area of natural language processing, most prominently automatic speech recognition and machine translation. As a development and test bed for this we have chosen three less-resourced African languages from the Bantu family: Basaa, Myene and Embosi. Work within the project is divided into three main steps: 1) Collection of a large corpus of speech (100h per language) at a reasonable cost. After initial recording, the data is re-spoken by a reference speaker to enhance the signal quality and orally translated into French. 2) Automatic transcription of the Bantu languages at phoneme level and the French translation at word level. The recognized Bantu phonemes and French words will then be automatically aligned. 3) Tool development. In close cooperation and discussion with the linguists, the speech and language technologists will design and implement tools that will support the linguists in their work, taking into account the linguists' needs and technology's capabilities. The data collection has begun for the three languages. For this we use standard mobile devices and a dedicated software—LIG-AIKUMA, which proposes a range of different speech collection modes (recording, respeaking, translation and elicitation). LIG-AIKUMA 's improved features include a smart generation and handling of speaker metadata as well as respeaking and parallel audio data mapping

Innovative technologies for under-resourced language documentation: The BULB Project

International audienceThe project Breaking the Unwritten Language Barrier (BULB), which brings together linguists and computer scientists, aims at supporting linguists in documenting unwritten languages. In order to achieve this we will develop tools tailored to the needs of documentary linguists by building upon technology and expertise from the area of natural language processing, most prominently automatic speech recognition and machine translation. As a development and test bed for this we have chosen three less-resourced African languages from the Bantu family: Basaa, Myene and Embosi. Work within the project is divided into three main steps: 1) Collection of a large corpus of speech (100h per language) at a reasonable cost. After initial recording, the data is re-spoken by a reference speaker to enhance the signal quality and orally translated into French. 2) Automatic transcription of the Bantu languages at phoneme level and the French translation at word level. The recognized Bantu phonemes and French words will then be automatically aligned. 3) Tool development. In close cooperation and discussion with the linguists, the speech and language technologists will design and implement tools that will support the linguists in their work, taking into account the linguists' needs and technology's capabilities. The data collection has begun for the three languages. For this we use standard mobile devices and a dedicated software—LIG-AIKUMA, which proposes a range of different speech collection modes (recording, respeaking, translation and elicitation). LIG-AIKUMA 's improved features include a smart generation and handling of speaker metadata as well as respeaking and parallel audio data mapping

Impact of Beneficial Microorganisms on Strawberry Growth, Fruit Production, Nutritional Quality, and Volatilome

Arbuscular mycorrhizal fungi (AMF) colonize the roots of most terrestrial plant species, improving plant growth, nutrient uptake and biotic/abiotic stress resistance and tolerance. Similarly, plant growth promoting bacteria (PGPB) enhance plant fitness and production. In this study, three different AMF (Funneliformis mosseae, Septoglomus viscosum, and Rhizophagus irregularis) were used in combination with three different strains of Pseudomonas sp. (19Fv1t, 5Vm1K and Pf4) to inoculate plantlets of Fragaria × ananassa var. Eliana F1. The effects of the different fungus/bacterium combinations were assessed on plant growth parameters, fruit production and quality, including health-promoting compounds. Inoculated and uninoculated plants were maintained in a greenhouse for 4 months and irrigated with a nutrient solution at two different phosphate levels. The number of flowers and fruits were recorded weekly. At harvest, fresh and dry weights of roots and shoots, mycorrhizal colonization and concentration of leaf photosynthetic pigments were measured in each plant. The following fruit parameters were recorded: pH, titratable acids, concentration of organic acids, soluble sugars, ascorbic acids, and anthocyanidins; volatile and elemental composition were also evaluated. Data were statistically analyzed by ANOVA and PCA/PCA-DA. Mycorrhizal colonization was higher in plants inoculated with R. irregularis, followed by F. mosseae and S. viscosum. In general, AMF mostly affected the parameters associated with the vegetative portion of the plant, while PGPB were especially relevant for fruit yield and quality. The plant physiological status was differentially affected by inoculations, resulting in enhanced root and shoot biomass. Inoculation with Pf4 bacterial strain increased flower and fruit production per plant and malic acid content in fruits, while decreased the pH value, regardless of the used fungus. Inoculations affected fruit nutritional quality, increasing sugar and anthocyanin concentrations, and modulated pH, malic acid, volatile compounds and elements. In the present study, we show for the first time that strawberry fruit concentration of some elements and/or volatiles can be affected by the presence of specific beneficial soil microorganisms. In addition, our results indicated that it is possible to select the best plant-microorganism combination for field applications, and improving fruit production and quality, also in terms of health promoting properties

Genome sequencing of Xanthomonas axonopodis pv. phaseoli CFBP4834-R reveals that flagellar motility is not a general feature of xanthomonads.

Xanthomonads are plant-associated bacteria that establish neutral, commensal or pathogenic relationships with plants. The list of common characteristics shared by all members of the genus Xanthomonas is now well established based on the entire genome sequences that are currently available and that represent various species, numerous pathovars of X. axonopodis (sensu Vauterin et al., 2000), X. oryzae and X. campestris, and many strains within some pathovars. These ?-proteobacteria are motile by a single polar flagellum. Motility is an important feature involved in biofilm formation, plant colonization and hence considered as a pathogenicity factor. X. axonopodis pv. phaseoli var. fuscans (Xapf) is one of the causal agents of common bacterial blight of bean and 4834-R is a highly aggressive strain of this pathogen that was isolated from a seed-borne epidemic in France in 1998. We obtained a high quality assembled sequence of the genome of this strain with 454-Solexa and 2X Sanger sequencing. Housekeeping functions are conserved in this genome that shares core characteristics with genomes of other xanthomonads: the six secretion systems which have been described so far in Gram negative bacteria are all present, as well as their ubiquitous substrates or effectors and a rather usual number of mobile elements. Elements devoted to the adaptation to the environment constitute an important part of the genome with a chemotaxis island and dispersed MCPs, numerous two-component systems, and numerous TonB dependent transporters. Furthermore, numerous multidrug efflux systems and functions dedicated to biofilm formation that confer resistance to stresses are also present. An intriguing feature revealed by genome analysis is a long deletion of 35 genes (33 kbp) involved in flagellar biosynthesis. This deletion is replaced by an insertion sequence called ISXapf2. Genes such as flgB to flgL and fliC to fleQ which are involved in the flagellar structure (rod, P- and L-ring, hook, cap and filament) are absent in the genome of strain 4834-R that is not motile. Primers were designed to detect this deletion by PCR in a collection of more than 300 strains representing different species and pathovars of Xanthomonas, and less than 5% of the tested xanthomonads strains were found nonmotile because of a deletion in the flagellum gene cluster. We observed that half of the Xapf strains isolated from the same epidemic than strain 4834-R was non-motile and that this ratio was conserved in the strains colonizing the next bean seed generation. Isolation of such variants in a natural epidemic reveals that either flagellar motility is not a key function for fitness or that some complementation occurs within the bacterial population. (Résumé d'auteur