Diseño e implementación de combinadores de potencia para amplificador LINC @ 868MHz

The ISM is a free band generally used in the environmental scientific and medical study. The regulation depends on the government of each country. This study is focused on the European¡¯s level frequency of 868 MHz, this frequency does not require standards or permissions, as long as they do not exceed the regulations. The project was based on the realization of a LINC transmitter with class E amplifiers made of passive matching networks. Also, the design and production of three combiners: Hybrid 90¨¬, Wilkinson and Chireix. In this project we have seen the different existing types of amplifiers (Class A, Class B ...) and the two main transistors that we can find in the market (BJT and FET). The amplifier¡¯s design was done using the ADS program from Agilent, and to characterize matching networks we have used the Smith Chart and ADS LineCalc. The first thing was find the polarization voltages, to characterize the S parameters of the transistor. The stability calculations have been made as theoretical as graphically (ADS). Matching networks are realized with passive elements such as coils and capacitors, that has been the difference of previous studies. At this point of the project the tunning tool was used to refine the results. The Bias Network were designed with shock coils and decoupling capacitors. The last step was the design of the layout with ADS software and then assembled. The other branch of the study has been the design and implementation of three combiners, for the simulation of both the ADS program was also used and finally took the design to a board. The results obtained for the amplifier with matching networks made of passive elements were good only for one channel. For this reason we have had to continue the combiners study with a project implemented earlier by another student, made with ¥ë / 4 lines. As we have seen in the laboratory, the Chireix combiner is positioned as the most efficient and which allows the maximum gain. Future studies may try to improve the performance of a class E type LINC amplifier with stable matching networks made of passive elements, as well as, improve the efficiency of the Chireix combiner and open new ways of study.La banda ISM es una banda libre que generalmente se usa en el estudio medioambiental, científico y médico. La normativa depende del gobierno de cada territorio. Este estudio se ha centrado en el ámbito europeo a la frecuencia de 868 MHz. Esta frecuencia no requiere de estándares ni permisos siempre que no supere la normativa

Genome-wide DNA copy number analysis of acute lymphoblastic leukemia identifies new genetic markers associated with clinical outcome

Altres ajuts: FUCALHH 2013; HUS272U13; GRS 994/A/14, BIO/SA10/14, BIO/SA31/13; Fundación Española de Hematología y Hemoterapia (FEHH), Universidad Pedagógica y Tecnológica de Colombia 223-2011Identifying additional genetic alterations associated with poor prognosis in acute lymphoblastic leukemia (ALL) is still a challenge. Aims: To characterize the presence of additional DNA copy number alterations (CNAs) in children and adults with ALL by whole-genome oligonucleotide array (aCGH) analysis, and to identify their associations with clinical features and outcome. Array-CGH was carried out in 265 newly diagnosed ALLs (142 children and 123 adults). The NimbleGen CGH 12x135K array (Roche) was used to analyze genetic gains and losses. CNAs were analyzed with GISTIC and aCGHweb software. Clinical and biological variables were analyzed. Three of the patients showed chromothripsis (cth6, cth14q and cth15q). CNAs were associated with age, phenotype, genetic subtype and overall survival (OS). In the whole cohort of children, the losses on 14q32.33 (p = 0.019) and 15q13.2 (p = 0.04) were related to shorter OS. In the group of children without good- or poor-risk cytogenetics, the gain on 1p36.11 was a prognostic marker independently associated with shorter OS. In adults, the gains on 19q13.2 (p = 0.001) and Xp21.1 (p = 0.029), and the loss of 17p (p = 0.014) were independent markers of poor prognosis with respect to OS. In summary, CNAs are frequent in ALL and are associated with clinical parameters and survival. Genome-wide DNA copy number analysis allows the identification of genetic markers that predict clinical outcome, suggesting that detection of these genetic lesions will be useful in the management of patients newly diagnosed with ALL

Spread of a SARS-CoV-2 variant through Europe in the summer of 2020

[EN] Following its emergence in late 2019, the spread of SARS-CoV-21,2 has been tracked by phylogenetic analysis of viral genome sequences in unprecedented detail3,4,5. Although the virus spread globally in early 2020 before borders closed, intercontinental travel has since been greatly reduced. However, travel within Europe resumed in the summer of 2020. Here we report on a SARS-CoV-2 variant, 20E (EU1), that was identified in Spain in early summer 2020 and subsequently spread across Europe. We find no evidence that this variant has increased transmissibility, but instead demonstrate how rising incidence in Spain, resumption of travel, and lack of effective screening and containment may explain the variant’s success. Despite travel restrictions, we estimate that 20E (EU1) was introduced hundreds of times to European countries by summertime travellers, which is likely to have undermined local efforts to minimize infection with SARS-CoV-2. Our results illustrate how a variant can rapidly become dominant even in the absence of a substantial transmission advantage in favourable epidemiological settings. Genomic surveillance is critical for understanding how travel can affect transmission of SARS-CoV-2, and thus for informing future containment strategies as travel resumes.S

Role of age and comorbidities in mortality of patients with infective endocarditis

[Purpose]: The aim of this study was to analyse the characteristics of patients with IE in three groups of age and to assess the ability of age and the Charlson Comorbidity Index (CCI) to predict mortality. [Methods]: Prospective cohort study of all patients with IE included in the GAMES Spanish database between 2008 and 2015.Patients were stratified into three age groups:<65 years,65 to 80 years,and ≥ 80 years.The area under the receiver-operating characteristic (AUROC) curve was calculated to quantify the diagnostic accuracy of the CCI to predict mortality risk. [Results]: A total of 3120 patients with IE (1327 < 65 years;1291 65-80 years;502 ≥ 80 years) were enrolled.Fever and heart failure were the most common presentations of IE, with no differences among age groups.Patients ≥80 years who underwent surgery were significantly lower compared with other age groups (14.3%,65 years; 20.5%,65-79 years; 31.3%,≥80 years). In-hospital mortality was lower in the <65-year group (20.3%,<65 years;30.1%,65-79 years;34.7%,≥80 years;p < 0.001) as well as 1-year mortality (3.2%, <65 years; 5.5%, 65-80 years;7.6%,≥80 years; p = 0.003).Independent predictors of mortality were age ≥ 80 years (hazard ratio [HR]:2.78;95% confidence interval [CI]:2.32–3.34), CCI ≥ 3 (HR:1.62; 95% CI:1.39–1.88),and non-performed surgery (HR:1.64;95% CI:11.16–1.58).When the three age groups were compared,the AUROC curve for CCI was significantly larger for patients aged <65 years(p < 0.001) for both in-hospital and 1-year mortality. [Conclusion]: There were no differences in the clinical presentation of IE between the groups. Age ≥ 80 years, high comorbidity (measured by CCI),and non-performance of surgery were independent predictors of mortality in patients with IE.CCI could help to identify those patients with IE and surgical indication who present a lower risk of in-hospital and 1-year mortality after surgery, especially in the <65-year group

Diseño e implementación de combinadores de potencia para amplificador LINC @ 868MHz

The ISM is a free band generally used in the environmental scientific and medical study. The regulation depends on the government of each country. This study is focused on the European¡¯s level frequency of 868 MHz, this frequency does not require standards or permissions, as long as they do not exceed the regulations. The project was based on the realization of a LINC transmitter with class E amplifiers made of passive matching networks. Also, the design and production of three combiners: Hybrid 90¨¬, Wilkinson and Chireix. In this project we have seen the different existing types of amplifiers (Class A, Class B ...) and the two main transistors that we can find in the market (BJT and FET). The amplifier¡¯s design was done using the ADS program from Agilent, and to characterize matching networks we have used the Smith Chart and ADS LineCalc. The first thing was find the polarization voltages, to characterize the S parameters of the transistor. The stability calculations have been made as theoretical as graphically (ADS). Matching networks are realized with passive elements such as coils and capacitors, that has been the difference of previous studies. At this point of the project the tunning tool was used to refine the results. The Bias Network were designed with shock coils and decoupling capacitors. The last step was the design of the layout with ADS software and then assembled. The other branch of the study has been the design and implementation of three combiners, for the simulation of both the ADS program was also used and finally took the design to a board. The results obtained for the amplifier with matching networks made of passive elements were good only for one channel. For this reason we have had to continue the combiners study with a project implemented earlier by another student, made with ¥ë / 4 lines. As we have seen in the laboratory, the Chireix combiner is positioned as the most efficient and which allows the maximum gain. Future studies may try to improve the performance of a class E type LINC amplifier with stable matching networks made of passive elements, as well as, improve the efficiency of the Chireix combiner and open new ways of study.La banda ISM es una banda libre que generalmente se usa en el estudio medioambiental, científico y médico. La normativa depende del gobierno de cada territorio. Este estudio se ha centrado en el ámbito europeo a la frecuencia de 868 MHz. Esta frecuencia no requiere de estándares ni permisos siempre que no supere la normativa

Genome-wide DNA copy number analysis of acute lymphoblastic leukemia identifies new genetic markers associated with clinical outcome

Identifying additional genetic alterations associated with poor prognosis in acute lymphoblastic leukemia (ALL) is still a challenge. Aims: To characterize the presence of additional DNA copy number alterations (CNAs) in children and adults with ALL by whole-genome oligonucleotide array (aCGH) analysis, and to identify their associations with clinical features and outcome. Array-CGH was carried out in 265 newly diagnosed ALLs (142 children and 123 adults). The NimbleGen CGH 12x135K array (Roche) was used to analyze genetic gains and losses. CNAs were analyzed with GISTIC and aCGHweb software. Clinical and biological variables were analyzed. Three of the patients showed chromothripsis (cth6, cth14q and cth15q). CNAs were associated with age, phenotype, genetic subtype and overall survival (OS). In the whole cohort of children, the losses on 14q32.33 (p = 0.019) and 15q13.2 (p = 0.04) were related to shorter OS. In the group of children without good- or poor-risk cytogenetics, the gain on 1p36.11 was a prognostic marker independently associated with shorter OS. In adults, the gains on 19q13.2 (p = 0.001) and Xp21.1 (p = 0.029), and the loss of 17p (p = 0.014) were independent markers of poor prognosis with respect to OS. In summary, CNAs are frequent in ALL and are associated with clinical parameters and survival. Genome-wide DNA copy number analysis allows the identification of genetic markers that predict clinical outcome, suggesting that detection of these genetic lesions will be useful in the management of patients newly diagnosed with ALL.The research leading to these results has received funding from the European Union’s Seventh Framework Programme (FP7/2007-2013) under grant agreement n°306242-NGS-PTL, the Fundación Castellano Leonesa de Hematología y Hemoterapia (FUCALHH 2013), the Consejería de Educación, Junta de Castilla y León (HUS272U13), Proyectos de Investigación del SACYL, Spain: GRS 994/A/14, BIO/SA10/14, BIO/SA31/13. The work was partially supported by grants from the Spanish Fondo de Investigaciones Sanitarias FIS 09/01543, PI12/00281, COST Action EuGESMA (BM0801), Fundación Española de Hematología y Hemoterapia (FEHH), and by grants (RD12/0036/0069) from the Red Temática de Investigación Cooperativa en Cáncer (RTICC), Instituto de Salud Carlos III (ISCIII), the Spanish Ministry of Economy and Competitiveness and the European Regional Development Fund (ERDF) “Una manera de hacer Europa” (Innocampus; CEI-2010-1-0010). MFC was supported by study commission (no. 223-2011) granted by the Universidad Pedagógica y Tecnológica de Colombia, Colombia.Peer Reviewe