Molecular prototypes for spin-based CNOT quantum gates

We show that a chemically engineered structural asymmetry in [Tb2] molecular clusters renders the two weakly coupled Tb3+ spin qubits magnetically inequivalent. The magnetic energy level spectrum of these molecules meets then all conditions needed to realize a universal CNOT quantum gate.Comment: 4 pages, 4 figure

Effect on Health-related Quality of Life of changes in mental health in children and adolescents

<p>Abstract</p> <p>Background</p> <p>The objective of the study was to assess the effect of changes in mental health status on health-related quality of life (HRQOL) in children and adolescents aged 8 - 18 years.</p> <p>Methods</p> <p>A representative sample of Spanish children and adolescents aged 8-18 years completed the self-administered KIDSCREEN-52 questionnaire at baseline and after 3 years. Mental health status was measured using the Strengths and Difficulties Questionnaire (SDQ). Changes on SDQ scores over time were used to classify respondents in one of 3 categories (improved, stable, worsened). Data was also collected on gender, undesirable life events, and family socio-economic status. Changes in HRQOL were evaluated using effect sizes (ES). A multivariate analysis was performed to identify predictors of poor HRQOL at follow-up.</p> <p>Results</p> <p>Response rate at follow-up was 54% (n = 454). HRQOL deteriorated in all groups on most KIDSCREEN dimensions. Respondents who worsened on the SDQ showed the greatest deterioration, particularly on Psychological well-being (ES = -0.81). Factors most strongly associated with a decrease in HRQOL scores were undesirable life events and worsening SDQ score.</p> <p>Conclusions</p> <p>Changes in mental health status affect children and adolescents' HRQOL. Improvements in mental health status protect against poorer HRQOL while a worsening in mental health status is a risk factor for poorer HRQOL.</p

AAV-mediated ERdj5 overexpression protects against P23H rhodopsin toxicity

Rhodopsin misfolding caused by the P23H mutation is a major cause of autosomal dominant retinitis pigmentosa (adRP), to date there are no effective treatments for adRP. The BiP co-chaperone and reductase ERdj5 (DNAJC10) is part of the ER quality control machinery and previous studies have shown that overexpression of ERdj5 in vitro enhanced the degradation of P23H rhodopsin; whereas knockdown of ERdj5 increased P23H rhodopsin ER retention and aggregation. Here, we investigated the role of ERdj5 in photoreceptor homeostasis in vivo by using an Erdj5 knock-out mouse crossed with the P23H knock-in mouse, and by adeno associated viral (AAV) vector-mediated gene augmentation of ERdj5 in P23H-3 rats. Electroretinogram (ERG) and optical coherence tomography (OCT) of Erdj5−/− and P23H+/−:Erdj5−/− mice showed no effect of ERdj5 ablation on retinal function or photoreceptor survival. Rhodopsin levels and localisation were similar to those of control animals at a range of time points. By contrast, when AAV2/8-ERdj5-HA was subretinally injected into P23H-3 rats, analysis of the full field ERG suggested that overexpression of ERdj5 reduced visual function loss 10 weeks post-injection. This correlated with a significant preservation of photoreceptor cells at 4 and 10 weeks post-injection. Assessment of the outer nuclear layer (ONL) morphology showed preserved ONL thickness and reduced rhodopsin retention in the ONL in the injected superior retina. Overall, these data suggest that manipulation of the ER quality control and ERAD factors to promote mutant protein degradation could be beneficial for the treatment of adRP caused by mutant rhodopsin

Zn2+ ion surface enrichment in doped iron oxide nanoparticles leads to charge carrier density enhancement

Here, we report the development of monodisperse Zn-doped iron oxide nanoparticles (NPs) with different amounts of Zn (ZnxFe3-xO4, 0 < x < 0.43) by thermal decomposition of a mixture of zinc and iron oleates. The as-synthesized NPs show a considerable fraction of wüstite (FeO) which is transformed to spinel upon 2 h oxidation of the NP reaction solutions. At any Zn doping amounts, we observed the enrichment of the NP surface with Zn2+ ions, which is enhanced at higher Zn loadings. Such a distribution of Zn2+ ions is attributed to the different thermal decomposition profiles of Zn and Fe oleates, with Fe oleate decomposing at much lower temperature than that of Zn oleate. The decomposition of Zn oleate is, in turn, catalyzed by a forming iron oxide phase. The magnetic properties were found to be strongly dependent on the Zn doping amounts, showing the saturation magnetization to decrease by 9 and 20% for x = 0.05 and 0.1, respectively. On the other hand, X-ray photoelectron spectroscopy near the Fermi level demonstrates that the Zn0.05Fe2.95O4 sample displays a more metallic character (a higher charge carrier density) than undoped iron oxide NPs, supporting its use as a spintronic material

Differential light-induced responses in sectorial inherited retinal degeneration.

Retinitis pigmentosa (RP) is a group of genetically and clinically heterogeneous inherited degenerative retinopathies caused by abnormalities of photoreceptors or retinal pigment epithelium in the retina leading to progressive sight loss. Rhodopsin is the prototypical G-protein-coupled receptor located in the vertebrate retina and is responsible for dim light vision. Here, novel M39R and N55K variants were identified as causing an intriguing sector phenotype of RP in affected patients, with selective degeneration in the inferior retina. To gain insights into the molecular aspects associated with this sector RP phenotype, whose molecular mechanism remains elusive, the mutations were constructed by site-directed mutagenesis, expressed in heterologous systems, and studied by biochemical, spectroscopic, and functional assays. M39R and N55K opsins had variable degrees of chromophore regeneration when compared with WT opsin but showed no gross structural misfolding or altered trafficking. M39R showed a faster rate for transducin activation than WT rhodopsin with a faster metarhodopsinII decay, whereas N55K presented a reduced activation rate and an altered photobleaching pattern. N55K also showed an altered retinal release from the opsin binding pocket upon light exposure, affecting its optimal functional response. Our data suggest that these sector RP mutations cause different protein phenotypes that may be related to their different clinical progression. Overall, these findings illuminate the molecular mechanisms of sector RP associated with rhodopsin mutations

Requisits del transport de mostres de diagnòstic per garantir l’estabilitat de les seves propietats biològiques

Mostres de diagnòstic; Conservació i transport; NormativaMuestras de diagnóstico; Conservación y transporte; NormativaDiagnostic samples; Conservation and transport; RegulationsAquest document s’aplica a les mostres de diagnòstic que es trameten entre un mòdul d’obtenció de mostres fins als laboratoris clínics processadors i també entre dos laboratoris. El document dóna una sèrie de recomanacions sobre les condicions adequades per al transport de les mostres de diagnòstic amb aquests objectius: • Preservar la integritat de les mostres de diagnòstic amb la finalitat de mantenir l’estabilitat de les propietats biològiques que les componen. Per aquest motiu, al document es troben una sèrie de consideracions sobre les variables mediambientals que poden afectar-les, com disminuir la seva influència i les referides al temps i la forma de transport, des de l’obtenció de la mostra, fins al processament al laboratori clínic destinatari. • Exposar una sèrie de requisits referits a la preparació i col·locació dels diferents tipus de contenidors, i les condicions idònies d’embalatge, etiquetatge i senyalització, de manera que formen part del conjunt de mesures organitzatives i de bona praxi. • Referir-se al contingut documental i dels registres que s’haurien de preparar i adjuntar a les mostres de diagnòstic, amb la finalitat de demostrar la traçabilitat del procediment de transport des del moment de la obtenció de la mostra fins a la recepció final al laboratori clínic processador, respectant la llei de confidencialitat de les dades. • Acomplir les condicions i els requisits de seguretat per disminuir o minimitzar el risc que pot comportar als manipuladors implicats en el transport de les mostres, ja sigui personal transportista, la societat o el medi ambient davant d’un accident mentre es porta a terme el transport de les mostres de diagnòstic

Solvent-induced on/off switching of intramolecular electron transfer in a cyanide-bridged trigonal bipyramidal complex

A cyanide-bridged [Co3Fe2] cluster with trigonal bipyramidal geometry shows solvent-driven reversible on/off switching of its thermally induced electron-transfer-coupled spin transition (ETCST) behaviour

Field-induced single-ion magnetic behaviour in a highly luminescent Er3+ complex

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