Looking out for the Gravettian in Greece

The number of sites and the chronostratigraphic record of the Upper Palaeolithic in Greece does not encourage major overviews. Still, aspects of regional differentiation and temporal variation in the exploitation of resources can be addressed – if only in a preliminary manner – through the currently available data. The region of northwestern Greece offers an opportunity to investigate the character of Gravettian cultural data in this part of the Balkan peninsula. Intensification of research will no doubt enrich the corpus of data and support wider intra- and inter-regional comparisons.Le nombre de sites et les données chrono-stratigraphiques relatifs au Paléolithique supérieur en Grèce n’encourage pas de vastes synthèses. Cependant, quelques particularités régionales et une variation diachronique dans l’exploitation des ressources peuvent être présentées –d’une façon très préliminaire- à partir des données actuellement disponibles. Le nord-ouest de la Grèce offre une opportunité d’analyser les particularités de la culture gravettienne dans cette partie de la péninsule balkanique. Il n’est pas douteux que le développement des recherches enrichira cette base documentaire et permettra de plus larges comparaisons intra et inter-régionales

Manipulation tactics of patients with neurotic disorders in everyday life and during therapy

Aim. The objective of the study was to examine the repertoire and intensity of manipula-tion tactics of neurotic patients in everyday life and during therapy, as well as diagnosing the intensity of Machiavellianism in neurotic patients. Methods. There were 111 study subjects: 44 patients with diagnosed neurotic disorders, 44 people from the control group and 23 therapists. The manipulation tactics were measured by means of survey methods of E. Mandal and D. Kocur and Machiavellianism was measured using the MACH-IV scale of M. Christi and F. Geis. Results. In comparison to people from the control group, the patients were more willing to use manipulation tactics such as guilt induction, threatening to break up the relationship, and self-mutilation but less willing to use supplication/begging. The intensity of tendency to undertake manipulation was higher in everyday life than during therapy. The Machiavellian-ism of patients was positively correlated with the tendency to employ manipulation tactics. Differences within the scope of general Machiavellianism between the patients and the control group were not noted. Conclusions. The manipulation tactics of neurotic patients are of morbid nature. They are related to anxiety, feeling of guilt and hostility. The tendency to manipulate correlates with Machiavellianism

Earthworm uptake routes and rates of ionic Zn and ZnO nanoparticles at realistic concentrations, traced using stable isotope labeling

The environmental behavior of ZnO nanoparticles (NPs), their availability to, uptake pathways by, and biokinetics in the earthworm Lumbricus rubellus were investigated using stable isotope labeling. Zinc isotopically enriched to 99.5% in 68Zn (68Zn-E) was used to prepare 68ZnO NPs and a dissolved phase of 68Zn for comparison. These materials enabled tracing of environmentally relevant (below background) NP additions to soil of only 5 mg 68Zn-E kg–1. Uptake routes were isolated by introducing earthworms with sealed and unsealed mouthparts into test soils for up to 72 h. The Zn isotope compositions of the soils, pore waters and earthworms were then determined using multiple collector inductively coupled plasma mass spectrometry. Detection and quantification of 68Zn-E in earthworm tissue was possible after only 4 h of dermal exposure, when the uptake of 68Zn-E had increased the total Zn tissue concentration by 0.03‰. The results demonstrate that at these realistic exposure concentrations there is no distinguishable difference between the uptake of the two forms of Zn by the earthworm L. rubellus, with the dietary pathway accounting for ∼95% of total uptake. This stands in contrast to comparable studies where high dosing levels were used and dermal uptake is dominant

Shape and charge of gold nanomaterials influence survivorship, oxidative stress and moulting of Daphnia magna

Engineered nanomaterials (ENMs) are materials with at least one dimension between 1–100 nm. The small size of ENMs results in a large surface area to volume ratio, giving ENMs novel characteristics that are not traditionally exhibited by larger bulk materials. Coupled with large surface area is an enormous capacity for surface functionalization of ENMs, e.g., with different ligands or surface changes, leading to an almost infinite array of variability of ENMs. Here we explore the effects of various shaped (spheres, rods) and charged (negative, positive) gold ENMs on Daphnia magna (D. magna) in terms of survival, ENM uptake and production of reactive oxygen species (ROS), a key factor in oxidative stress responses. We also investigate the effects of gold ENMs binding to the carapace of D. magna and how this may induce moulting inhibition in addition to toxicity and stress. The findings suggest that ENM shape and surface charge play an important role in determining ENM uptake and toxicity

Shorter Disease Duration Is Associated With Higher Rates of Response to Vedolizumab in Patients With Crohn's Disease But Not Ulcerative Colitis.

Background & aimsPatients with Crohn's disease (CD), but not ulcerative colitis (UC), of shorter duration have higher rates of response to tumor necrosis factor (TNF) antagonists than patients with longer disease duration. Little is known about the association between disease duration and response to other biologic agents. We aimed to evaluate response of patients with CD or UC to vedolizumab, stratified by disease duration.MethodsWe analyzed data from a retrospective, multicenter, consortium of patients with CD (n = 650) or UC (n = 437) treated with vedolizumab from May 2014 through December 2016. Using time to event analyses, we compared rates of clinical remission, corticosteroid-free remission (CSFR), and endoscopic remission between patients with early-stage (≤2 years duration) and later-stage (>2 years) CD or UC. We used Cox proportional hazards models to identify factors associated with outcomes.ResultsWithin 6 months initiation of treatment with vedolizumab, significantly higher proportions of patients with early-stage CD, vs later-stage CD, achieved clinical remission (38% vs 23%), CSFR (43% vs 14%), and endoscopic remission (29% vs 13%) (P < .05 for all comparisons). After adjusting for disease-related factors including previous exposure to TNF antagonists, patients with early-stage CD were significantly more likely than patients with later-stage CD to achieve clinical remission (adjusted hazard ratio [aHR], 1.59; 95% CI, 1.02-2.49), CSFR (aHR, 3.39; 95% CI, 1.66-6.92), and endoscopic remission (aHR, 1.90; 95% CI, 1.06-3.39). In contrast, disease duration was not a significant predictor of response among patients with UC.ConclusionsPatients with CD for 2 years or less are significantly more likely to achieve a complete response, CSFR, or endoscopic response to vedolizumab than patients with longer disease duration. Disease duration does not associate with response vedolizumab in patients with UC

PLANTAS ALIMENTÍCIAS NÃO CONVENCIONAIS: UTILIZAÇÃO DAS FOLHAS DE “ORA-PRO-NOBIS” (PERESKIA ACULEATA MILL, CACTACEAE) NO CONSUMO HUMANO.

A ora-pro-nobis (Pereskia aculeata) é uma cactácea popularmente conhecida como “carne de pobre” devido ao seu uso popular pelo teor de proteína encontrado em suas folhas e seu baixo custo, sendo classificada como uma Planta Alimentícia Não Convencional (PANC). Possui ampla distribuição, sendo encontrada principalmente na América Central, Índia Oriental, América do Norte e no Brasil, reforçando a sua característica de ser facilmente cultivada sem depender de um solo fértil, conferindo à Pereskia aculeata um notável potencial para sua aplicação na prevenção e no tratamento de condições relacionadas a deficiências nutricionais. Tendo em vista todos os benefícios da planta, o objetivo geral do trabalho consistiu em analisar a composição química e nutricional da “ora-pro-nobis” a fim de difundir sua utilização à população vegetariana e vegana, sendo os objetivos específicos o desenvolvimento de uma farinha e aplicação de um questionário para avaliar o perfil de consumo da cactácea. Para isso, as folhas de ora-pro-nobis foram coletadas na cidade de Campo Largo e, em sequência, higienizadas e passadas por um processo de quarteamento para subsequente amostragem. Foram feitas análises morfológicas superficiais, determinações de umidade, de proteínas, de lipídios, de fibras, de minerais e de carboidratos, seguindo as metodologias do Instituto Adolfo Lutz (IAL) e a Association of Official Agricultural Chemists (AOAC), e aplicação de um questionário por meio da plataforma Google Formulários. A partir disso, os resultados para proteínas encontrados foram relativamente mais baixos quando comparados à literatura, sendo isso justificado pela sazonalidade. Por fim, pode-se concluir a partir dos resultados que a Pereskia aculeata supre o papel como fonte alternativa de proteínas se consumida na forma de farinha. Além disso, obteve-se no questionário que o consumo mais prevalente da ora-pro-nobis era in natura ou na forma de saladas, reforçando a importância da divulgação do presente trabalho acadêmico à comunidade externa, visto que consumindo a cactácea na sua forma fresca, não se obtém uma quantidade significante de proteínas

Experience with telemedicine among rheumatology clinicians during the COVID-19 pandemic: an international survey

Objective: The aim was to assess rheumatology clinicians' perceptions of telemedicine and their experiences before and during the coronavirus disease 2019 (COVID-19) pandemic. Methods: We conducted a cross-sectional online survey and collected responses from rheumatology clinicians worldwide, between November 2020 and February 2021, regarding use and perceptions of telemedicine in rheumatology. We summarized data with descriptive statistics and qualitative analysis for free-text responses. Results: The survey was completed by 349 rheumatology clinicians from 49 countries; 59% were female and about two-thirds were in the 30-50 years age group. Academic affiliations were held by 55% of participants, and 44% were from North America. Before the pandemic, 24% of participants had experience with telemedicine, whereas about three-quarters used telemedicine for the first time during the pandemic. Overall, 56% thought they provided less adequate care with telemedicine. More than half of clinicians felt that telemedicine was adequate for evaluating crystalline arthritis, inflammatory arthritis and lupus flares. Telemedicine was felt to be inadequate for flares of myositis, vasculitis and scleroderma. Technical problems were reported in 29% of telemedicine encounters and were most commonly related to patient-encountered difficulties. Conclusion: Most rheumatology clinicians used telemedicine for the first time during the pandemic. The quality of care provided was thought to be inferior to that provided in person for specific clinical situations. Additional efforts are needed to address barriers to effective telemedicine, such as patient-related technology issues, challenges with building rapport and performing a physical examination, and to define the appropriate scope of clinical scenarios conducive to telemedicine

Cooperation between CYB5R3 and NOX4 via coenzyme Q mitigates endothelial inflammation

NADPH oxidase 4 (NOX4) regulates endothelial inflammation by producing hydrogen peroxide (H2O2) and to a lesser extent O2•-. The ratio of NOX4-derived H2O2 and O2•- can be altered by coenzyme Q (CoQ) mimics. Therefore, we hypothesize that cytochrome b5 reductase 3 (CYB5R3), a CoQ reductase abundant in vascular endothelial cells, regulates inflammatory activation. To examine endothelial CYB5R3 in vivo, we created tamoxifen-inducible endothelium-specific Cyb5r3 knockout mice (R3 KO). Radiotelemetry measurements of systolic blood pressure showed systemic hypotension in lipopolysaccharides (LPS) challenged mice, which was exacerbated in R3 KO mice. Meanwhile, LPS treatment caused greater endothelial dysfunction in R3 KO mice, evaluated by acetylcholine-induced vasodilation in the isolated aorta, accompanied by elevated mRNA expression of vascular adhesion molecule 1 (Vcam-1). Similarly, in cultured human aortic endothelial cells (HAEC), LPS and tumor necrosis factor α (TNF-α) induced VCAM-1 protein expression was enhanced by Cyb5r3 siRNA, which was ablated by silencing the Nox4 gene simultaneously. Moreover, super-resolution confocal microscopy indicated mitochondrial co-localization of CYB5R3 and NOX4 in HAECs. APEX2-based electron microscopy and proximity biotinylation also demonstrated CYB5R3's localization on the mitochondrial outer membrane and its interaction with NOX4, which was further confirmed by the proximity ligation assay. Notably, Cyb5r3 knockdown HAECs showed less total H2O2 but more mitochondrial O2•-. Using inactive or non-membrane bound active CYB5R3, we found that CYB5R3 activity and membrane translocation are needed for optimal generation of H2O2 by NOX4. Lastly, cells lacking the CoQ synthesizing enzyme COQ6 showed decreased NOX4-derived H2O2, indicating a requirement for endogenous CoQ in NOX4 activity. In conclusion, CYB5R3 mitigates endothelial inflammatory activation by assisting in NOX4-dependent H2O2 generation via CoQ.This work was supported by National Institutes of Health (NIH) R01 awards [R01 HL 133864 (A.C.S), R01 HL 128304 (A.C.S), R01 HL 149825 (A.C.S), R01 HL 153532 (A.C.S), R01 GM 125944 (F.J.S.), R01 DK 112854 (F.J.S.), 1S10OD021540-01 (Center for Biologic Imaging, University of Pittsburgh), 1S10RR019003-01 (Simon Watkins (S.W.)), 1S10RR025488-01 (S.W.), 1S10RR016236-01 (S.W)]. American Heart Association (AHA) [Established Investigator Award 19EIA34770095 (A.C.S.)], Post-doctoral Fellowship 19POST34410028 (S.Y.)]. American Society of Hematology (ASH) Minority Hematology Graduate Award (A.M.D-O.). Junta de Andalucía grant BIO-177 (P.N.), the FEDER Funding Program from the European Union and Spanish Ministry of Science, Innovation and Universities grant RED2018-102576-T (P.N.)

Spatial structure and temporal dynamics of the human occupation of Neuquén (Argentinian Patagonia) during the late Pleistocene-Holocene

Se generó una base de datos conformada por 184 fechados georeferenciados procedentes de sitios arqueológicos de la provincia del Neuquén. Doce de estos fechados -realizados a partir de restos óseos humanos y faunísticos- son originales y corresponden, algunos de ellos, a áreas geográficas para las que aún no se contaba con datos. Se efectuaron análisis cuantitativos temporo-espaciales. Los resultados obtenidos sobre la base de datos cronológicos y geográficos permiten caracterizar el proceso de ocupación humana de Neuquén como temprano, mayormente continuo a lo largo del tiempo y espacialmente discontinuo. La profundidad temporal del poblamiento alcanza ca. 12.000 años calibrados AP y la ocupación del espacio se incrementa significativamente hacia el Holoceno tardío. La distribución espacial de los sitios es discontinua y muestra concentraciones en áreas particulares cercanas a los cursos de agua permanente, mayoritariamente peri-cordilleranas, en tanto que en los espacios intermedios -las amplias planicies del este- no se registró una señal arqueológica marcada.A database was generated consisting of 184 georeferenced dates from archaeological sites in Neuquén Province. Twelve of these dates −taken from human and faunal bone remains− are original, and some correspond to geographical areas for which data were not previously available. Quantitative spatial-temporal analyzes were carried out. The results obtained on the basis of chronological and geographical data allow Neuquén’s human occupation process to be characterized as early, mostly continuous over time, and spatially discontinuous. The temporal depth of the settlement reaches ca. 12,000 years BP calibrated, with spacial occupation increasing significantly towards the late Holocene. The spatial distribution of the sites is discontinuous and shows concentrations in particular areas near the permanent water courses, mostly near the Andes, while in the intermediate spaces −the wide plains of the east− a marked archaeological signal was not recorded.Fil: Gordón, Florencia. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División Antropología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Beguelin, Marien. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigación en Paleobiología y Geología; ArgentinaFil: Rindel, Diego Damián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Secretaría de Cultura de la Nación. Dirección Nacional de Cultura y Museos. Instituto Nacional de Antropología y Pensamiento Latinoamericano; ArgentinaFil: Della Negra, Claudia Eugenia. No especifíca;Fil: Hajduk, Adam. Administración de Parques Nacionales. Museo de la Patagonia "Francisco Perito Moreno"; ArgentinaFil: Vazquez, Romina Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigación en Paleobiología y Geología; ArgentinaFil: Cobos, Virginia Agustina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Departamento Científico de Antropología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Perez, Sergio Ivan. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Departamento Científico de Antropología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Bernal, Valeria. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Departamento Científico de Antropología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentin

Stratifying Disease Progression in Patients With Cardiac ATTR Amyloidosis

Background: Transthyretin cardiac amyloidosis (ATTR-CA) is a progressive cardiomyopathy. The clinical course varies among individuals and there are no established measures to assess disease progression. Objectives: The goal of this study was to assess the prognostic importance of an increase in N-terminal pro-B-type natriuretic peptide (NT-proBNP) and outpatient diuretic intensification (ODI) as markers of disease progression in a large cohort of patients with ATTR-CA. Methods: We evaluated landmark survival analysis based on worsening of NT-proBNP and requirement for ODI between time of diagnosis and a 1-year visit, and subsequent mortality in 2,275 patients with ATTR-CA from 7 specialist centers. The variables were developed in the National Amyloidosis Centre (NAC) cohort (n = 1,598) and validated in the external cohort from the remaining centers (n = 677). Results: Between baseline and 1-year visits, 551 (34.5%) NAC patients and 204 (30.1%) patients in the external validation cohort experienced NT-proBNP progression (NT-proBNP increase >700 ng/L and >30%), which was associated with mortality (NAC cohort: HR: 1.82; 95% CI: 1.57-2.10; P < 0.001; validation cohort: HR: 1.75; 95% CI: 1.32-2.33; P < 0.001). At 1 year, 451 (28.2%) NAC patients and 301 (44.5%) patients in the external validation cohort experienced ODI, which was associated with mortality (NAC cohort: HR: 1.88; 95% CI: 1.62-2.18; P < 0.001; validation cohort: HR: 2.05; 95% CI: 1.53-2.74; P < 0.001). When compared with patients with a stable NT-proBNP and stable diuretic dose, a higher risk of mortality was observed in those experiencing either NT-proBNP progression or ODI (NAC cohort: HR: 1.93; 95% CI: 1.65-2.27; P < 0.001; validation cohort: HR: 1.94; 95% CI: 1.36-2.77; P < 0.001), and those experiencing both NT-proBNP progression and ODI (NAC cohort: HR: 2.98; 95% CI: 2.42-3.67; P < 0.001; validation cohort: HR: 3.23; 95% CI: 2.17-4.79; P < 0.001). Conclusions: NT-proBNP progression and ODI are frequent and consistently associated with an increased risk of mortality. Combining both variables produces a simple, universally applicable model that detects disease progression in ATTR-CA