A Survey of Linguistic Studies in Five Major Science Funds in China(2005-2014)

对中国五大科研基金2005—2014年十年间语言学课题立项情况进行的调查分析发现,国家自然科学基金重视计算机的语言文字处理能力,全国教育科学规划课题重视语言学习问题,而国家哲学社会科学基金、教育部哲学社会科学规划课题、国家语委科研规划课题更多侧重语言本体等相关问题。所有立项课题体现了国家需要和国家需求。文章提出了一些有关语言研究和语言管理的思考。This article conducts a content analysis of the fi ve major science funds in China in an attempt to examine the guidelines and topics of linguistic studies funded by the fi ve major funds between 2005 and 2014. The study describes the general situation of the fi ve major funds, traces the directions of the funded research projects, and focuses on the hot topics in the "applied social sciences" domains. The result shows that National Natural Science Fund favors computational linguistics and language processing abilities, National Educational Science Fund focuses on language studies, and National Social Science Fund, Humanities & Social Science Fund of MOE and Fund of National Language Committee prioritize corpus studies. The authors conclude that the funded projects of linguistic studies refl ect the research demand at a national level. Based on these fi ndings, the article provides thoughts and implications for language research and language policy making processes

基于栖息地风险评价模型的海岸带滩涂湿地风险评价——以闽三角为例

海岸带湿地是生物多样性最丰富、生产力最高、最具价值的生态系统之一。然而近年来,随着城市化和工业化进程的加快,我国海岸带地区的土地利用正发生深刻变化,生态风险凸显。利用2000、2005、2010和2015年的Landsat TM/ETM+和Landsat 8 OLI遥感影像,融合行政区划图与地形图,并引入一个评价多元海洋、海岸带利用对栖息地造成风险的模型,即Integrated Valuation of Ecosystem Services and Trade-offs(In VEST)模型中的栖息地风险评价(Habitat Risk Assessment,HRA),模型评价闽三角海岸带滩涂湿地的生态风险。结果表明:(1)闽三角海岸带滩涂湿地风险以低风险为主;(2)泉州地区的风险面积最大(约4389.91 hm~2),漳州地区最小(约4630.73 hm~2);(3)修正面积影响的情况下,其他建设用地造成的暴露和影响较大,耕地造成的暴露和影响最小。不同区域滩涂湿地风险程度的可视化表达,可以揭示滩涂湿地高风险地区以及高风险的产生原因,便于管理者对滩涂湿地采取精准的保育措施。国家重点研发计划(2016YFC0502900);;福建省科学技术厅民生科技专项(引导性项目)(2017Y6002);;福建省自然科学基金资助项目(2015J01122

我国外汇储备规模的影响因素分析——基于协整分析的实证研究

本文使用协整分析方法对我国外汇储备规模的影响因素进行实证研究。结果表明外汇储备规模与出口总额、进口总额、外商直接投资、短期外债余额和人民币汇率等影响因素之间存在长期的协整关系。本文最后对我国外汇储备管理提出了相关的政策建议

紧密连接蛋白claudins应用于肿瘤治疗的进展

Claudins蛋白家族是组成紧密连接(Tight junctions,TJs)必不可少的骨架蛋白,在维持上皮和内皮细胞中的细胞极性、细胞间的粘附固定、细胞旁路的离子运输等发挥重要作用。近年来大量的研究结果证明,claudins在许多人类恶性肿瘤中异常表达。因此,claudins也被作为癌症治疗的潜在靶标。文中就claudin蛋白家族在肿瘤中的表达情况及其相关药物的研究进展进行阐述。国家自然科学基金(No.31870925);;\n传染病重大专项(No.2017ZX10202203-001)资助~

再循环抗体的研究进展

单克隆抗体因其与抗原结合具有高度特异性与强亲和力,已成为抗体药物研发的主要类型。但随着天然单克隆抗体的深入研究,它的诸多缺陷也浮出水面,如与抗原结合次数有限、带来非预期的抗体清除效应和抗原累积效应。人们不再局限于天然抗体的筛选,而是想通过改造提升抗体药物的药效。近年来,一类新型再循环抗体的问世,很好地解决了天然单克隆抗体发展的瓶颈。再循环抗体可以在胞外结合抗原,在细胞内与抗原解离,使抗体结合抗原次数最大化,减少抗原介导的抗体清除效应和抗体介导的抗原累积效应,并且再循环抗体可以通过进一步的Fc改造来加强与Fc受体的亲和力。文中综述了再循环抗体的研究进展,包括其特点、改造方法及展望。国家自然科学基金(No.31600748);;福建省自然科学基金(No.2017J01066);;传染病重大专项(No.2017ZX10202203-001-001)资助~

Establishment of MDCK cell lines which stably express visualable human neonatal Fc receptor

[目的]建立稳定表达融合EGFP的人新生儿Fc受体（h FcRn）的MDCK细胞株。[方法]构建重组慢病毒质粒p EGFP-h FcRn,采用四质粒包装系统共转染HEK 293T细胞生产重组慢病毒,感染MDCK细胞后对EGFP阳性细胞进行流式单细胞分选;通过Western Blot及EGFP-β2m荧光共定位验证h FcRn的完整性,并用流式细胞仪检测h FcRn与人Ig G的结合活性。[结果]测序结果表明成功构建p EGFP-FcRn慢病毒表达载体;感染后EGFP阳性MDCK细胞比例约为26.5%,流式单细胞分选后得到纯阳性细胞;荧光共定位及Western Blot均检测到h FcRn的完整表达;流式分析表明细胞株上的h FcRn与Ig G存在p H依赖性结合。[结论]成功获得稳定表达具有生物活性的可视化h FcRn的MDCK细胞株。[ Objective] To establish MDCK cell line stably expressing EGFP- human neonatal Fc receptor（hFcRn） fusion protein. [ Methods ] The lentiviral expression vector for EGFP - hFcRn fusion protein was constructed. Generating by co - transfection of four -plasmids into HEK 293T cells ,the lentivirus particles were used to infect MDCK cell line. EGFP positive single cell was obtained by FACS, and then FcRn expression was identified by fluorescence co -location with EGFP - β2m and confirmed by Western Blot. Flow cytometry was used to detect binding activity of hFcRn and human IgG. [ Results ] DNA se- quencing demonstrated that the lentivirus vector pEGFP - FcRn was constructed successfully. The percentage of EGFP - posi- tive ceils was about 26.5% after infection. Expression of the complete protein was detected through fluorescence co - location and Western Blot, respectively. Flow cytometry analysis showed that the cell lines could pH - dependently capture human IgG. [ Conclusion] MDCK cell line stably expressing functional visualable hFcRn was established.基金项目：国家自然科学基金项目（“结构生物学指导的HBV治疗性抗体人源化及其关键技术研究”,No.31600748;“抗呼吸道合胞病毒高中和活性抗体的保护机制研究”,No.81401668;“基于广谱中和单抗的通用型流感疫苗设计及其结构基础研究”,No.31670934

高致病性禽流感病毒血凝素蛋白广谱中和表位模拟肽的筛选与鉴定

以H5N1型禽流感病毒HA蛋白广谱中和单抗8H5为基础,利用噬菌体展示肽库技术及类病毒颗粒融合表达技术研究HA模拟表位。ELISA检测结果显示:筛选获得模拟HA表位的模拟肽123,进行类病毒颗粒融合蛋白表达后,仍具有与8H5单抗特异结合的能力。免疫荧光检测结果说明,类病毒颗粒免疫小鼠后产生了能与HA交叉反应的抗体。禽流感病毒HA模拟表位的研究与性质的分析及类病毒颗粒融合蛋白的表达与活性分析、免疫原性分析,都为研制禽流感通用表位疫苗奠定了基础

高致病性禽流感病毒H5N1中和抗体的单链抗体构建与活性鉴定

在本实验室研制出的多株针对H5N1血凝素的鼠单抗中,10F7对34株H5N1病毒株都有血凝抑制和中和活性,具有特异性高、反应性强、识别谱广的特点。通过基因工程构建10F7单链抗体(scFv)表达重组质粒,在大肠杆菌中表达并纯化scFv,经血凝抑制实验及中和实验检测其活性。结果在针对3株病毒的血凝抑制实验中,10F7scFv蛋白对其中2株H5N1病毒均显示出结合活性,而对H9毒株没有反应。在针对7株H5N1病毒的中和实验中,10F7scFv对5株病毒具有较好的中和能力。H5N1广谱中和抗体10F7的单链抗体构建,为进一步研制针对H5N1禽流感病毒的治疗性抗体奠定了基础

Therapeutic effects comparison between platelet-rich plasma and sodium hyaluronate intra-articular injection in treatment of knee osteoarthritis

目的关节腔内注射自体富血小板血浆（PRP）与透明质酸钠（SH）,观察并比较其疗效。方法于2012年2月至12月,漳州市解放军第175医院骨科收治的81例Kellgren-Lawrence（KL）分级标准为Ⅱ、Ⅲ级膝关节骨关节炎的患者,年龄48～60岁,平均（52.6±3.6）岁;男性27例,女性53例;左膝38例,右膝43例。K-L分级标准,Ⅱ级47例,Ⅲ级34例,按就诊顺序序贯随机平行分为SH组（40例）和PRP组（41例）,分别采用SH和PRP关节腔内注射进行治疗。治疗前和治疗后3、6、9、12个月对患者进行随访并进行美国西部Ontario与Mc Master大学骨关节炎指数（WOMAC）评分评估,两组间各时间点WOMAC评分比较采用t检验,两组内各时间点WOMAC评分比较采用重复测量方差分析;分别于治疗前和治疗后12个月进行膝关节磁共振检查,评价关节腔内积液的吸收情况。结果治疗前及治疗后3个月PRP组WOMAC评分[（48.42±4.95）分,（25.38±5.12）分]和SH组[（47.17±5.12）分,（25.17±5.09）分]比较,t值分别为-0.625和0.413,差异无统计学意义（P均〉0.05）;治疗后6、9、12个月PRP组WOMAC评分[（21.44±4.73）分,（20.54±4.46）分,（16.53±7.35）分]和SH组[（25.87±5.16）分,（27.22±4.21）分,（29.63±8.21）分]比较,PRP组低于SH组,t值分别为-2.765、-3.447和-4.657,差异具有统计学意义（P均〈0.05）,治疗后12个月复查磁共振,可见PRP组的关节积液吸收例数高于SH组,差异有统计学意义（P〈0.05）。结论膝关节腔内注射自体PRP,临床效果优于透明质酸钠,中长期疗效好。Objective To compare the efficacy of platelet rich plasma （PRP） and sodium hyaluronate （SH） intra-articular injection in the patients with knee osteoarthritis of grade Ⅱand Ⅲ. Methods A total of 81 patients with knee osteoarthritis of grade Ⅱ and Ⅲ （ Kellgren-Lawrence standard） were randomly assigned to the PRP and SH group. All 81 patients were treated in the 175th hospital of PLA, Zhangzhon, with the average age of （52.6 ± 3.6） years. There were 27 males and 53 females. Forty cases in SH group and 41 cases in PRP group had got the complete follow-up. Both the two groups adopted 4 ml of 5% lidocaine for intra-articular injection. All the patients were prospectively evaluated before the treatment and at the 34 , 6th , 9th and 12th month after the treatment by the Western Ontario and McMaster Universities （WOMAC） osteoarthritis index. The swelling subsiding knee was observed. Between the two groups WOMAC scores were compared using t test at each time point, within the groups the scores were compared by repeated measures analysis of variance. The absorption of accumulated fluid in the joint cavity was observed and compared by MRI before and 12 months after the treatment. Results No serious adverse event was detected in the two groups. According to the follow-up evaluations, both the groups presented clinical improvements. The comparison between the two groups showed no statistically significant difference before therapy and three months after the treatment （ t =- 0. 625, 0. 413, P 〉 0. 05 ）. Six months, nine months and 12 months after the treatment, WOMAC score in the PRP group[ （21.44 ±4. 73）, （20. 54 ± 4. 46） ,（16. 53 ± 7. 35 ） pointsl were lower than the SH group[（25.87 ±5.16）, （27.22 ±4.21）, （29. 63 ± 8.21 ） points] ; the difference was statistically signifieant（ t = - 2. 765, - 3. 447, - 4. 657, P 〈 0. 05 ）. Conclusion PRP intra-artieular injection in the treatment of knee osteoarthritis of gradeⅡ and Ⅲ may be more suitable compared to南京军区医学科技创新重点课题项目（15ZD027

乙型肝炎病毒pre-S1区中和抗体可变区的原核表达

鼠源单克隆抗体MA18 7是特异识别乙型肝炎病毒 (HBV)pre S1抗原的中和抗体。为表达MA18 7的小分子抗体 ,将MA18 7的重链可变区基因 (VH)和轻链可变区 (VL)基因分别克隆到原核表达载体pTO T7进行原核表达。结果VH 和VL 均以包涵体的形式高效表达 ,包涵体经过变性、复性及金属离子亲和纯化后获得纯度 >90 %的重组抗体片段。生物传感器、竞争ELISA和间接ELISA测定均显示 ,VH 或VL 均不能结合相应抗原 ,但二者体外混合后可迅速非共价结合形成具有良好活性的可变区抗体Fv,表明MA18 7的抗原结合活性区由重链可变区和轻链可变区共同组