福建邵武将石地区维管束植物区系分析

福建邵武将石地区的植物区系处于泛北极植物区,中国-日本森林植物亚区的华东地区,有维管束植物173科461属915种(含种下单位).该区植物种类丰富,具有许多珍稀、古老、孑遗植物成分.在科、属水平上对其区系特征进行了分析,结果表明:热带分布类型的科占绝对优势,共77科,占53.1%;温带分布类型有37科,占25.5%;属的区系成分中,属的热带分布类型计197属,占区总属数的52.2%,温带分布类型共有169属,占44.9%.可见该植物区系的热带、亚热带成分占主导地位,温带成分次之.聚类分析结果表明该地区种子植物区系成分和庐山自然保护区关系最为密切,鼎湖山自然保护区次之,和五台山自然保护区的差异较大,这主要由它们生态地理条件、区系成分组成的差异所决定

打出监管“组合拳” 促进年报高质量披露

背景材料:近日,中国注册会计师协会(简称"中注协")发布的数据显示,截至2019年2月25日,共有38家事务所向中注协报备了上市公司财务报表审计机构变更信息,涉及上市公司277家。对于年报披露期上市公司"临阵换将",市场疑虑重重。此前,监管部门曾通过召开年报监管工作会、业务培训、约谈、现场走访、问询等方式"敲警钟"。中注协也书面约谈中审众环会计师事务所,提示临近会计期末更换会计师事务所的上市

Emission Mechanism of High Al-content AlGaN Multiple Quantum Wells

紫外LED的发光功率和效率还远不能令人们满意,波长短于300 nm的深紫外LED的发光效率普遍较低。厘清高Al组分Al Ga N多量子阱结构的发光机制将有利于探索改善深紫外LED的发光效率的新途径、新方法。为此,本文通过金属有机气相外延技术外延生长了表面平整、界面清晰可辨且陡峭的高Al组分AlGa N多量子阱结构材料,并对其进行变温光致发光谱测试,结合数值计算,深入探讨了Al Ga N量子阱的发光机制。研究表明,量子阱中具有很强的局域化效应,其发光和局域激子的跳跃息息相关,而发光的猝灭则与局域激子的解局域以及位错引起的非辐射复合有关。The quantum efficiency of deep UV light emitting diodes( LED) drops dramatically with the increasing of Al content. Understanding the emission mechanism of high Al-content Al Ga N multiple quantum wells( MQW) is the one of the most important objects for improving the quantum efficiency of deep UV LED. In this work,high Al-content Al Ga N MQW structure with atomically flat hetero-interfaces was grown and characterized by photoluminescence( PL) measurements at different temperatures. The results indicate that there is a strong exciton-localization effect in the MQW structure and the emission is closely related to the hopping of the excitons. Due to the exciton delocalization and nonradiative recombination at defects,the PL intensity is strongly quenched at high temperatures.“973”国家重点基础研究发展计划(2012CB619300);; “863”国家高技术研究发展计划(2014AA032608);; 国家自然科学基金(U1405253;61227009;11204254;11404271);; 福建省自然科学基金(2015J01028)资助项

Wood Anatomy Characteristics of 6 Mangrove Species of Sonneratia and Their Application

应用光镜及扫描电镜对生长在热带、亚热带海岸潮间带的 6种海桑属红树植物的次生木质部进行了解剖学研究表明 :海桑属 6种红树植物的次生木质部具有导管直径小 ,导管分布频率高 ,复孔率高 ,导管壁厚和纤维壁较厚且具次生加厚 ,木薄壁组织缺乏或稀少等与环境相适应的特点。海桑属 6种红树植物的数量特征还表明 :海桑、无瓣海桑、卵叶海桑的木材可能适合造纸。The study reveals that the secondary xylem of 6 mangrove species of Sonneratia possesses the following characteristics:the diameter of vessel is small,the distribution frequency of vessel is high,the reperforating rate is high,the wall of vessel is thick,the wall of fibre is relatively thick and the wood parenchyma is short or sparse,whish is suitable with the environment.The quantitative feature of 6 mangrove species of Sonneratia also indicates that the wood of S.caseolaria,apetalous sonneratia and ovate-leaf sonneratia is probably suitable for paper-making.国家自然科学基金资助项目! (495 76 2 95

Studies on chemical constituents in ethanolic extract from Acanthus ilicifolius as a pharmaceutic mangrove

目的探讨老鼠簕的化学成分。方法将老鼠簕全株粉碎后,用95%乙醇浸提3次,合并浓缩,浸膏的石油醚部位经反复硅胶柱层析分离,结合波谱学数据鉴定化合物结构。结果从石油醚部位分离得到8个化合物,分别为β-谷甾醇(Ⅰ)、豆甾醇(Ⅱ)、正十六烷酸(Ⅲ)、正二十八烷酸(Ⅳ)、4,22-二烯-3-酮豆甾烷(stig-masta-4,22-dien-3-one)(Ⅴ)、4-烯-3-酮豆甾烷(stigmast-4-en-3-one)(Ⅵ)、5,22-二烯-7-酮-3β-羟基豆甾烷(3β-hydroxystigmasta-5,22-dien-7-one)(Ⅶ)、2-唑啉酮(2-benzoxazolinone)(Ⅷ)。结论其中Ⅴ,Ⅵ,Ⅶ为首次从该植物中分离得到。Objective To study the chemical constituents of Acanthus ilicifolius.Methods The air-dried and powdered plant material was extracted with 95% ethanol.Silica gel and Sephadex LH-20 column chromotography were used in the isolation from the ethanol extracts of the whole plant,the compounds were determined on the basis of various modern spectroscopic analyses and physical constants.Results Eight compounds were isolated from the petroleum ether soluble portion,identified as β-sitosterol(Ⅰ),stigmasterol(Ⅱ),n-hexadecane acid(Ⅲ),n-octacosanic acid(Ⅳ),stigmasta-4,22-dien-3-one(Ⅴ),stigmasta-4-en-3-one(Ⅵ),3β-hydroxystigmasta-5,22-dien-7-one(Ⅶ) and 2-benzoxazolinone(Ⅷ).Conclusion CompoundⅤ,Ⅵ,Ⅶ were obtained from the genus for the first time.国家自然科学基金(30671646);; 福建省科技计划资助项目(2003Y036

Elemene reversed the multidurg resistance of A549 /DDP lung cancer cells via mitochondrial apoptosis pathway

目的:探讨榄香烯乳(ElEMEnE,ElE)逆转人肺腺癌耐顺铂(CISPlATIn,ddP)细胞A549/ddP的耐药性及作用机制。方法:采用MTT法检测榄香烯乳单用的细胞毒作用及与ddP合用时耐药逆转作用。荧光探针JC-1结合激光共聚焦显微镜检测线粒体膜电位的变化。dCfH-dA荧光探针结合流式细胞仪检测细胞内活性氧(rEACTIVE OXygEn SPECIES,rOS)水平。用谷胱甘肽试剂盒结合分光光度法检测计算gSH/(gSSg+gSH)比值。蛋白质印迹法检测胞质中CyTO C、PrO-CASPASE-3、CASPASE-3和bCl-2家族蛋白表达情况。结果:不同浓度榄香烯乳抑制A549/ddP细胞株生长,呈时间-剂量依赖性效应,联合顺铂能提高A549/ddP细胞株对顺铂的敏感性而逆转耐药。不同浓度榄香烯乳联合顺铂使A549/ddP细胞株线粒体膜电位下降,rOS浓度增加,gSH/(gSSg+gSH)比值降低,上调胞质中CyTO C、CASPASE-3、bAd蛋白表达,下调PrO-CASPASE-3、bCl-2蛋白表达。结论:榄香烯乳逆转A549/ddP细胞株耐药性可能与其损伤线粒体膜,活化胞内氧化还原体系,诱导线粒体凋亡路径有关。Objective: To explore the mechanism that elemene( ELE) reversed the multidurg resistance( MDR)of A549 /DDP lung adenocarcinoma cell.Methods: MTT assay was used to determine the growth inhibition of human lung adenocarcinoma A549 /DDP cells in vitro.Mitochondrial membrane potential( MMP) was monitored by JC- 1fluorescence probe with laser confocal scanning microscopy,the intercellular reactive oxygen species( ROS) level was measured by 2',7'- dichlorfluorescein- diacetate( DCFH- DA) staining and flow cytometry and the ratio of GSH /( GSSG +GSH) was calculated according to detection results of GSH kit.The expression of Cytochrome C,Caspase-3 and the Bcl- 2 family proteins and in the case of cyclosporine A and DEVD- CHO,the expression of Caspase- 3expression were measured by Western blot.Results: MTT results showed that different concentrations ELE could inhibit the proliferation of A549 /DDP cells in a time- and dose- dependent manner.Intriguingly,ELE plus cisplatin enhanced the sensitivity of A549 /DDP cells to cisplatin and reversed A549 /DDP cells dury resistance.Different concentrations ELE decreased mitochondrial membrane potential,increased intracellular ROS concentration and decreased GSH /( GSSG + GSH) ratio of A549 /DDP cells in a time- and dose- dependent manner.Furthermore,the combination with both ELE and cisplatin also enhanced the protein expression of Cytoplasmic C,Caspase- 3 and Bad,and reduced the protein levels of Bcl- 2 and Pro- caspase- 3 in the cisplatin- resistant A549 /DDP cancer cells.Conclusion: ELE reversed the MDR of A549 /DDP cell line may demage mitochondrial membrane,active intracellular redox system and induce the mitochondrial apopotosis pathway.福建省自然科学基金面上项目(编号:2010D014

Dynamic Evolution of Regional Ecological Services Based on TM Remote Sensing

针对传统评估区域生态系统服务价值空间异质性的问题,本研究将TM遥感影像与价值评估法相结合,测算出针对研究区域的当量因子表,并建立了只需遥感影像数据即可快速并较为精准地动态计算区域生态系统服务价值的多元回归模型。文章以北京市永定河、厦门市和青海省不同尺度为例,对该方法评估区域生态系统服务价值的可行性和准确性进行了讨论,以期为生态综合管理提供定量化参考依据。This paper discussed the dynamic evaluating of regional ecological services with remote sensing technique,based on the comprehensive analysis the character of indexes method and currently equivalent method applying to regional ecological services.With the established method in the literature,it discussed the practical possibility for evaluation the temporal and special regional ecological services in Yongding River,Xiamen and Qinghai province.Compared with the traditional static indexes evaluation,our method discussed the possibility of dynamically evolutional evaluation,in order to provide reference of ecosystem management.北京市科学技术委员会“永定河生态修复目标体系研究”(D090409004009003);北京市生态学重点学科项

圣·约翰草提取物片治疗抑郁障碍专家共识

结合循证医学证据,本文归纳圣·约翰草提取物片治疗抑郁障碍的专家共识

Experimental study on reperfusion of intraocular lens

作者简介: 祁明信, 男, 1945 年7 月 出生, 教授、主任医师、博士研究生 导师, 主要从事白内障的基础与临 床研究。联系电话: 0591-83570887; E-mail:qihuang@netease. com 通讯作者: 黄秀榕,E-mail:[email protected][中文文摘]目的开展晶状体再灌注的离体和动物实验研究,并对再灌注人工晶状体技术进行评价。方法采用新鲜离体幼兔眼、离体猪眼、新西兰白兔眼,应用自行研制的人工晶状体材料,进行以下实验:(1)体外固化实验;(2)晶状体前囊膜微型撕囊及其稳固性实验;(3)经微型前囊膜开口超声乳化吸出晶状体内容物实验;(4)活的新西兰白兔眼内人工晶状体再灌注实验。结果(1)按硅酮聚合物与固化剂50:1的比例可获得柔软、弹性好、固化时间短(完全固化时间为60min)的注入材料;(2)晶状体前囊膜1.8～2.0mm的连续环形撕囊口具有较好的稳定性,可经该微型开口吸出晶状体内容物并灌注材料;(3)超声能量18%、流量25mL·min-1、负压120mmHg(1kPa=7.5mmHg)为晶状体内容物经微型前囊膜开口吸出的最佳条件;(4)注入灌注材料后可形成由晶状体囊膜包裹的、置换原晶状体皮质和核的、新的再灌注人工晶状体。结论采用再灌注人工晶状体的方法可进行新型人工晶状体再灌注,可为治疗白内障和老视提供参考。[英文文摘]Objective To carry out the experimental study on reperfusion of intraocular lens(IOLs) in vitro or in animal,and to assess the technique of IOLs reperfusion.Methods The following experiments were performed by using self-developed materials in fresh rabbit eyes and pig eyes in vitro,as well as in eyes of alive New-Zea-land rabbits:(1)Solidification study of self-developed material in vitro;(2)Continuous circular capsulorhexis(CCC) in anterior capsule of lens and its stability;(3)Draw of lens contents via phaco through mini-CCC;(4)IOLs ref illing in the eyes of alive New-Zea land rabbits. Results(1) Thematerialwhich was soft, springy and short-term solidification(full solidification time was 60 minutes) were obtained in certain proportion of geland solidified agent(50:1) in vitro; ( 2)The CCC in anterior capsule of lens with 1.8-2.0 mm diameter had very good stability. The lens contents were drawn and the materialwere refilled through themini-CCC; (3) The best conditions of drawing out lens contents through m ini-CCC were phaco energy 18% , flow 25 mL·min- 1, and negative pressure 120 mmH g (1kPa=7.5 mmHg);(4) The new refilled IOLs, which were wrapped by capsule of lens and were replaced original cortex and nucleus of lens, were obtained after thematerial refillied. Conc lusion. New IOLs are refilled through this method, which can prov ide reference for the treatment of cataract and presbyopia.福建省科技三项费用;教育厅重点资助项目基金资助(编号:K98041