Screen of Virus-eliminated Seedlings of Narcissus tazella var. chinesis Roen

以水仙离体试管芽为材料 ,采用 0 .2～ 0 .3mm微茎尖培养、37± 1℃热力处理 15d与 30d +0 .2～ 0 .3mm微茎尖培养 ,37± 1℃热力处理 5 0d、75d、10 0d等脱毒方法 ,应用组织细胞化学法和ELISA法进行初筛、复检、复筛等程序的定性检测 .结果表明 :从 10 80瓶 (每瓶为 1个芽系 )组培苗中检出 4瓶无毒苗 ;再经ELISA法的定量检测结果 ,初步认为采用热力处理对试管芽有一定的脱毒效果 ,试管芽经 37± 1℃热力处理 30d +0 .2～ 0 .3mm微茎尖培养方法有良好脱毒效果Narcissus in vitro tube-buds were used to treat with the different virus-elimination techniques: meristem culture(0.2～0.3 mm), heat treat (37±1 ℃) 15 d + meristem culture (0.2～0.3 mm), heat treat (37±1 ℃) 50 d, 75 d, 100 d. The result of qualitative and quantitative detection by histochemical method and ELISA showed virus in vitro tube-buds were treated by heat (37±1 ℃) 30 d + meristem culture (0.2～0.3 mm). The elimination were better effects after they were treated.漳州市科技计划项目 (Z9713);; 福建省科技项目(99 Z 10 5

Study on mutation breeding of banana buds in vitro Ⅳ: Bio-chemical analysis to 'Zhangjiao No. 8' strain

本文用60COγ射线对“台湾北蕉”(Musa AAA Group Cavendish)辐照诱变选育的“漳蕉8号”株系抗损伤相关酶的生化指标和过氧化物酶同工酶进行分析,结果表明,“漳蕉8号”株系对60COγ射线造成的损伤已完成修复,其优良性状可稳定遗传。Treated with irradiation of 60Coγ ray, a new strain 'Zhangjiao No.8' was developed from 'Musa AAA Group Cavendish'. The bio-chemical indexes corresponding to anti-injury and POD isoenzyme of 'Zhangjiao No.8' were analyzed. The results showed that 'Zhangjiao No.8' strain had recovered from the irradiation injury and the excellent traits could be inherited stably.漳州市科技计划项目(Z9315

MUTATION BREEDING OF BANANA TUBE-BUDS IN VITRO Ⅴ. GENOMIC VARIATION OF ZHANGJIAO No.8 STRAIN DETECTED BY RAPD

应用 2 0条Sangon的随机引物 ,对6 0 Coγ射线辐照诱变所获新株系漳蕉 8号(原漳农 8号 )及其对照品种台湾北蕉 (MusAAAGrandCavendish)的总DNA进行RAPD ,结果发现有 1 2条引物扩增出 68条谱带 ,其中同源带 53条 ,占总带数的77 9% ,差异带 1 5条 ,占总带数的 2 2 1 % ;漳蕉 8号与对照多态性差异高达 2 4 6% ,且显示出稳定性遗传。其中S10 和S19的 3条差异带 ,可作为漳蕉 8号株系鉴定和防退化复壮的筛选标记。Zhangjiao No.8,a new variety induced from irradiation by 60 Co γ rays,was analyzed by RAPD using 20 random primers against Mus AAA Grand Cavendish. The result showed that 68 bands could be amplified by 12 primers,53 were homologous,accounted for 77 9% of total bands; only 22 1% were differential bands,the polymorphism difference up to 24 6% between Zhangjiao No.8 and CK,indicating its stable heredity.Three differential bands from S 10 and S 19 could be used as markers for identification and anti degeneration of Zhangjiao No.8

JUNO Sensitivity on Proton Decay p→νˉK+p\to \bar\nu K^+ Searches

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this paper, the potential on searching for proton decay in $p\to \bar\nu K^+$ mode with JUNO is investigated.The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits to suppress the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar\nu K^+$ is 36.9% with a background level of 0.2 events after 10 years of data taking. The estimated sensitivity based on 200 kton-years exposure is $9.6 \times 10^{33}$ years, competitive with the current best limits on the proton lifetime in this channel

JUNO sensitivity on proton decay p → ν K + searches*

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this study, the potential of searching for proton decay in the $p\to \bar{\nu} K^+$ mode with JUNO is investigated. The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits suppression of the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar{\nu} K^+$ is 36.9% ± 4.9% with a background level of $0.2\pm 0.05({\rm syst})\pm$$0.2({\rm stat})$ events after 10 years of data collection. The estimated sensitivity based on 200 kton-years of exposure is $9.6 \times 10^{33}$ years, which is competitive with the current best limits on the proton lifetime in this channel and complements the use of different detection technologies