以水仙离体试管芽为材料 ,采用 0 .2～ 0 .3mm微茎尖培养、37± 1℃热力处理 15d与 30d +0 .2～ 0 .3mm微茎尖培养 ,37± 1℃热力处理 5 0d、75d、10 0d等脱毒方法 ,应用组织细胞化学法和ELISA法进行初筛、复检、复筛等程序的定性检测 .结果表明 :从 10 80瓶 (每瓶为 1个芽系 )组培苗中检出 4瓶无毒苗 ;再经ELISA法的定量检测结果 ,初步认为采用热力处理对试管芽有一定的脱毒效果 ,试管芽经 37± 1℃热力处理 30d +0 .2～ 0 .3mm微茎尖培养方法有良好脱毒效果Narcissus in vitro tube-buds were used to treat with the different virus-elimination techniques: meristem culture(0.2～0.3 mm), heat treat (37±1 ℃) 15 d + meristem culture (0.2～0.3 mm), heat treat (37±1 ℃) 50 d, 75 d, 100 d. The result of qualitative and quantitative detection by histochemical method and ELISA showed virus in vitro tube-buds were treated by heat (37±1 ℃) 30 d + meristem culture (0.2～0.3 mm). The elimination were better effects after they were treated.漳州市科技计划项目 (Z9713);; 福建省科技项目(99 Z 10 5
