Design of Raman spectroscopy measurement system based on SHINERS

基于壳层隔绝纳米粒子增强技术,开发了一种新型的等离激元拉曼光谱测试系统,该系统能够有效地提高被测物质的拉曼反射信号强度。壳层隔绝纳米粒子增强技术采用包裹了极薄的二氧化硅或者氧化铝壳层的单层金纳米粒子,能够产生较强的表面增强效应。整个测试系统的硬件部分主要包括双处理器(ArM和dSP)主控板、半导体激光光源、光谱仪、光纤探头和粒子施加装置;软件部分能够自动获取被测物质的拉曼谱图。这里,粒子施加装置用于自动地将壳层隔绝纳米粒子施加到被测样本中。在实验中,将测试系统用于检测食品违禁添加物三聚氰胺和孔雀石绿,以验证壳层隔绝纳米粒子增强拉曼光谱技术。实验结果表明,该测试系统具有较高的测试灵敏度和较短的测试时间,广泛地适用于食品安全中痕量物质快速检测。In this paper,the Raman spectroscopy mea surement system was presented,where Shell isolated Nanoparticle Enhanced Raman Spectroscopy(SHINERS) technique was used to amplify the Raman signal.This technique used gold nanoparticles coated with a thin,uniform,fully enclosed and optically transparent shell of silica or alumina so that the gold core generated a large surface enhancement.Based on this technique,the hardware framework of this measurement system was mainly composed of double central control chips(ARM and DSP),a laser diode source,a spectrometer,a fiber probe and a nanoparticle giving device;and the software of this system can achieve the Raman spectrogram automatically.The giving device was used to spread the SHINERS particle into the samples for improving automation.The experiments were performed to detect the food added melamine and malachite green for verifying the effectiveness of SHINERS.The results show that the measurement system is of high detection sensitivity and short testing time effectively,which can be widely adopted in the rapid detection of trace substances in food safety.国家重大科学仪器设备开发专项(2011YQ03012417

Ionic mechanism underlying distinctive excitability in atrium and ventricle of the heart

心肌细胞兴奋性是维持正常的心脏活动的一个重要生理因素。本研究旨在使用全细胞膜片技术探讨豚鼠心房和心室肌细胞不同兴奋性的离子机理。结果显示,心室肌细胞兴奋性比心房肌细胞低。虽然豚鼠心室肌细胞的电压门控快nA+电流(InA)密度较低,但与其兴奋性较低并不相关,因为其在阀电位附近的可用度比率比心房肌细胞高。经典内向整流钾电流(Ik1)在心室肌细胞比在心房肌细胞更大,这可能是心室肌细胞兴奋性较低的部分原因。此外,去极化引起的有内向整流特性的瞬时外向钾电流(ITOIr)在心室肌细胞较大,并可能是其兴奋性较低的主要原因。在心室肌细胞,5μMOl/l bA2+显著抑制ITOIr,增强细胞兴奋性,并使InA激活的阈电位更负,其作用独立于对InA的影响。本研究结果证明,除经典的Ik1外,ITOIr在豚鼠心房肌和心室肌细胞的兴奋性差异形成和心肌兴奋性维持中起着主要作用。然而,ITOIr增加是否会通过降低兴奋性以保护心脏,还需要进一步研究。Cellular excitability is an important physiological factor in maintaining normal cardiac activity.The present study was designed to investigate the ionic mechanism underlying different excitability in atrial and ventricular myocytes of guinea pig heart using a whole-cell patch configuration.We found that excitability is lower in ventricular myocytes than that in atrial myocytes.Although the density of voltage-gated fast Na+ current(INa) was lower in ventricular myocytes, it would not correlate to the lower excitability since its availability was greater than that in atrial myocytes around threshold potential.Classical inward rectifier K+ current(IK1) was greater in ventricular myocytes than that in atrial myocytes, which might contribute in part to the lower excitability.In addition, the transient outward K+ current with inward rectification(Itoir) elicited by depolarization was greater in ventricular myocytes than that in atrial myocytes and might contribute to the lower excitability.In ventricular myocytes, Ba2+ at 5 μmol/L significantly inhibited Itoir, enhanced excitability, and shifted the threshold potential of INa activation to more negative, and the effect was independent of affecting INa.Our results demonstrate the novel information that in addition to classical IK1, Itoir plays a major role in determining the distinctive excitability in guinea pig atrial and ventricular myocytes and maintaining cardiac excitability.More effort is required to investigate whether increase of Itoir would be protective via reducing excitability.supportedbySunChiehYehHeartFoundationofHongKongandaGeneralResearchFund(HKU771712M)fromResearchGrantCouncilofHongKon

Thermal performance testing for high power light-emitting diode based on voltage-current characteristics with pulse injection

热学特性是影响功率型lEd光学和电学特性的主要因素之一,设计了一套基于脉冲式u-I特性的功率型lEd热学特性测试系统,可以测试在不同结温下lEd工作电流与正向电压的关系,从而获得lEd的热学特性参数。该系统通过产生窄脉冲电流来驱动lEd,对其峰值时的电压电流进行采样,同时控制和采集lEd的热沉温度,从而获得不同温度下lEd的u-I特性曲线。与其他u-I测试系统相比,文中采用了窄脉冲(1μS)工作电流,lEd器件Pn结区处于发热与散热的交替过程,不会造成大的热积累,大大提高了测量精度。实验中,对某功率型lEd进行了测试,获得了该器件的电压、电流和结温特性曲线,并利用b样条建立该器件的u-I-T模型,进而实现了对其结温的实时在线检测。Thermal performance is one of the main factors which affect the optical and electrical performance of high power LED.The thermal performance testing system for high power LEDs based on voltage-current characteristics with pulse injection was designed in this paper, which can test the relationship between the operating current and forward voltage of LED under different junction temperatures, thereby obtaining LED thermal characteristics parameters.The system worked by generating a controlled narrow pulse current to drive the LED, and sampling the peaks of voltage and current of LEDs with LED heat sink temperature control and acquisition, thereby obtaining the voltage-current characteristic curve in different junction temperatures.Compared with other voltage-current testing systems, the designed system adapts the narrow pulse duty cycle(1 μs), so the PN junction of LED devices is always in the process of alternately heating and cooling, which can avoid large heat accumulation and greatly improve the accuracy of measurement.In the experiment, a power LED device was tested by the system and the voltage-current-temperature curve was obtained.Then the B-spline based U-I-T model of the LED was established, so the real-time online detection of LED device was achieved.国家自然科学基金(61102030

Over-expression of Pygo2 Promotes C6 Cells Proliferation of Glioma

目的通过构建过表达PygO2的重组体上调PygO2表达,探讨其在大鼠胶质瘤C6细胞增殖中的作用及机制。方法重组体经ECOr I和HIndⅢ双酶切鉴定和dnA测序后,用脂质体2000将其转染大鼠胶质瘤C6细胞,采用WESTErn blOT检测外源PygO2蛋白表达,应用克隆形成实验和MTT法检测细胞增殖,流式细胞术检测细胞周期,采用WESTErn blOT检测过表达PygO2对C6细胞中CyClInd1、β-CATEnIn水平的影响,并采用细胞免疫荧光法检测其对C6细胞中CyClInd1、β-CATEnIn亚细胞定位的影响。结果双酶切和测序鉴定结果证实插入序列正确,重组体能有效上调PygO2表达。将重组体转染C6细胞上调PygO2表达后,细胞的生长增殖被显著促进,克隆形成显著增多,细胞周期进程从g1期至S期转变显著增强;且CyClInd1水平随之增高,亚定位无改变,β-CATEnIn水平和亚细胞定位无明显改变。结论成功构建了过表达PygO2的重组体,过表达PygO2通过增高CyClInd1水平,促进细胞从g1期进入S期,从而促进大鼠胶质瘤C6细胞增殖。Objective To up-regulate expression of Pygopus2(Pygo2) by construction of the recombinant vectors of over-expression of Pygo2 protein,and to explore the role and mechanism of over-expression of Pygo2 in C6 cells proliferation of glioma.Methods The recombinant plasmids were digested with EcoRⅠ and Hind Ⅲ to execute the restriction endonuclease identification,then the sequence analysis was assayed by DNA sequencing.The recombinant plasmids were transfected into cultured glioblastoma C6 cells using lipofectamineTM 2000.The exogenous Pygo2 protein level of C6 cells was detected by Western blot analysis.Colony forming assay and MTT assay were used to detect the cell proliferation,and cell cycle analysis was performed by flow cytometry analysis.The effect of Pygo2 over-expression on the level of cyclinD1 and β-catenin of C6 cells was detected by Western blot analysis,and the expression and subcellular location of cyclinD1 and β-catenin of C6 cells were further quantified by immunofluorescent staining.Results The recombinant plasmids were completely coincided with the designs by the restriction map and the sequence analysis,which up-regulated Pygo2 expression of C6 cells efficiently.After Pygo2 expression were up-regulated by transfected C6 cells with the recombinant plasmids,cells proliferation was promoted and colony forming was increased significantly,cell cycle progression from G1 to S transition was enhanced notably.Furthermore,the expression level of cyclinD1 was significantly increased without change of subcellular location,and the expression level and subcellular location of β-catenin were not changed obviously.Conclusion The recombinant vectors of Pygo2 over-expression were constructed successfully.Over-expression of Pygo2 promotes the growth of glioma cells by an increased expression of cyclinD1 to improve G1/S transition.重庆市自然科学基金资助项目(cstc2011jjA10110);重庆市教委科技基金资助项目(KJ100504);福建省自然科学基金资助项目(2009D002

The effect of nurse initiated patient/family education strategy on people with schizophrenia in Beijing

2004-2005 > Academic research: refereed > Publication in refereed journalVersion of RecordPublishe

Treatment of Purified Terephthalic Acid Wastewater by Sequencing Batch Reactor

［中文文摘］采用序批式反应器(SBR)处理模拟精对苯二甲酸(PTA)废水,考察了曝气量、沉降时间、进水方式等对对苯二甲酸(TA)生物降解效果的影响。实验结果表明,对于TA质量浓度小于1 500m g/L的废水,采用完全曝气SBR运行4h,TA和COD的去除率均能达到95%以上,TA平均去除速率随TA浓度的增加而增大。TA质量浓度为1 500m g/L时,曝气量、沉降时间和进水方式是影响其降解效果的主要因素。采用SBR处理高浓度PTA废水可克服污泥膨胀和抗冲击负荷能力弱的问题,且系统的稳定性和PTA废水的处理效果较好。［英文文摘］Simulated purified terephthalic acid(PTA)wastewater was treated by sequencing batch reactor(SBR).The effects of aeration rate,settling time,feeding mode on the biodegradation of terephthalic acid(TA)were investigated.When the mass concentration of TA is less than 1 500mg/L,the removal rate of TA and COD are both more than 95% after aerating for 4h in SBR.The average TA removal rate increases with the increasing of TA mass concentration.When the mass concentration of TA is 1 500mg/L,the main factors affec ting degrada tion are aeration rate, settling time and feeding mode. Treating high concentration PTA was tewater by SBR can solve the problems of sludge bulking and lack of shock load resistance cap ability, and the system has a good stability and treatment effect for PTA wastewater.国家自然科学基金资助项目(20076037

Bone Proteomic Analysis About Chinese Medicine Action On Rat Ovariectomy Model of Osteoporosis.

目的:用蛋白质组学的方法对绝经后骨质疏松症和中药作用机理进行研究,有利于临床防治该疾病。方法:建立去卵巢大鼠骨质疏松症模型,设立中药治疗组及假手术对照组,6周后骨质疏松症模型成功,行骨形态学检查,提取骨组织蛋白质样品,双向电泳分离,得到各组骨组织总蛋白质分子解剖图谱,用图像分析软件,分析各组间差异蛋白质点,MADITOF/MS质谱分析,结合蛋白质生物信息库(Matrix science Ltd database),对各蛋白质初步鉴定。结论:鉴定了3个差异蛋白,分别为P1硫氧还蛋白过氧化酶1(Thioredoxin peroxidase 1),P2为阻凝蛋白轻链肽2(Myosin light polypeptide 2),P3为泛素化酶E2-17KD(ubiquitin-conjugating enzyme E2-17kD)。初步认为这三种蛋白质在绝经后骨质疏松症的发病及中药治疗过程中发挥着重要调控作用。Objective:To study the mechanism about osteoporosis of OVX(ovariectomy) and Chinese Medicine action on osteoporosis in proteomics,it is of benefit to prevent and cure this disease.Methods:Bilateal OVX in rats was performed as osteoporosis model.Rat was randomly divided into three groups:control group,osteoporosis model group and Chinese Medicine therapy group.The pathology of bone was examined after 6 weeks.In the present proteomic study,we characterized the protential effects of OVX and Chinese Medicine on protein expression in rat bones.Using two-dimensional gel electrophoresis,mass spectrometry,and Matrix science Ltd database,we elementarily identified three variational proteins.Conclusions:Three proteins were identified as proteins similar to thioredoxin peroxidase 1,Myosin light polypeptide 2 and ubiquitin-conjugating enzyme E2-17kD.These proteins have been demonstrated to be postmenopausal proteins.These results can provide valuable experimental evidences for the research for the molecular mechanism of osteoporosis which was response to OVX and Chinese Medicine in bone.国家自然科学基金项目(基金号:30400606); 广东省自然科学基金项目(基金号:04010036);; 广东省科技厅项目(基金号:粤科社字2004,139号);; 广州中医药大学创新基金项目(基金号:K004044

Bone Proteomic Analysis About Chinese Medicine Action on Rat Glucocorticoid-induced Model of Osteonecrsis.

目的:用蛋白质组学的研究方法进行激素性骨坏死的病理和中药作用机理的研究,有利于临床治激素性骨坏死。方法:按照常用激素性骨坏死的造模方法,建立大鼠坏死模型,并设立中药治疗组及空白对照组,经过6周处理后经骨形态学检查,确定骨坏死造模成功,处死动物取股骨和肱骨,提取骨组织蛋白质样品,双向电泳分离,得到各组骨组织总蛋白质分子解剖图谱,用图像分析软件,找到各组间差异蛋白质点,进一步行胶内酶切,M AD ITOF/M S质谱分析,得到各差异点的蛋白质肽指纹图谱,结合蛋白质生物信息库(M atrix sc ience L td database),对各蛋白质进行初步鉴定。结论:初步鉴定了3个差异蛋白,分别为阻凝蛋白重链ⅡB、磷脂谷胱甘肽过氧分酶及泛素化酶E 2(MW:17 kd),初步认为这三种蛋白质在激素性骨坏死的发病及中药治疗过程中发挥着重要调控作用。Objective:To study the mechanism about glucocorticoid-induced osteonecrosis and Chinese Medicine action on osteonecrosis in proteomics,it is beneficial to prevention and cure this disease.Methods:Rat was randomly divided into three groups:cortrol group,osteonecrosis model group and Chinese Medicine therapy group.In the present proteomic study ,we characterized the potential effects of glucocorticoids and Chinese Medicine on protein expression in rat bone.Using two-dimensional gel electrophoresis,mass spectrometry,and Matrix science Ltd database,we elementarily identified three variational proteins.Conclusion:Three proteins were identified as proteins similar to Myosin heavy chain ⅡB,Phospholipid hydroperoxide glutathione peroxidase and ubiquitin-conjugating enzyme E2-17kD.These proteins have been documented to be glucocorticoid-relted proteins.These results can provide valuable experimental evidences for the research of the molecular mechanism of osteonecrosis response to glucocorticoids and Chinese Medicine in bone.获得国家自然科学基金资助(30273631,3010244);; 广东省自然科学基金(020778);; 广东省教育厅基金(Z02011

Template Synthesis and UV-Vis Absorption Spectra of the Nanowire Arrays of Cadmium Chalcogenides

以多孔氧化铝为模板 ,用交流电分别通过含有相应的CdCl2 、ZnCl2 、单质S、Se等的二甲亚砜 (DMSO)溶液 ,沉积CdS、CdSe以及CdxZn1-xS半导体纳米线阵列并研究其紫外可见吸收光谱 .实验结果表明 ,当半导体纳米线的直径小于 2 5nm时 ,其吸收边相对于体相的吸收边产生蓝移 ,而且蓝移的幅度随着半导体纳米线直径的减小而增加 ,显示了明显的量子限域效应 .XU Shi_min, XUE Kuan_hong , KONG Jing_lin, SUN Dong_mei, FENG Yu_ying, LU Hai_yan (Dept. of Chem., Nanjing Normal Univ., Nanjing 210097, China) WANG Guang_hou (National Laboratory of Solid State Microstructures, Nanjing Univ., Nanjing 210093, China)The porous alumina membrane formed in the anodic oxidation of highly pure aluminum foil has attracted a great deal of attention in recent years [1] . It can be served as a desired template to prepare nanometer scale materials [2] due to its unique structure of discrete and cylindrical nanopores, paralleled one another, with the homogeneous size and distribution [3] . Chalcogenide semiconductors have promising prospect in the applications of photovoltaic [4] and photoconducting devices [5] and have been extensively exploited for many years. Olbright and his co_workers studied experimentally and theoretically the optical nonlinearties of CdS xSe 1-x _doped glass [6] ; Britt and Ferekides reported that the conversion efficiency in a solar cell of thin_film CdS/CdTe could be as high as 15.8% [7] . Here we report the fabrication and UV_Vis absorption spectra of CdS、CdSe and Cd xZn 1-x S nanowire arrays deposited into the template matrix of porous alumina. The diameters of these nanowires were varied from 10 nm to 50 nm in our experiments.作者联系地址：南京师范大学化学系!江苏南京210097,南京师范大学化学系!江苏南京210097,南京师范大学化学系!江苏南京210097,南京师范大学化学系!江苏南京210097,南京师范大学化学系!江苏南京210097,南京师范大学化学系!江苏南京210097,南京大学固体微结构国家重点实验室!江苏南京21009

集美大学2002级福建籍新生肝炎感染现状

目的　了解集美大学 2 0 0 2级福建籍新生甲、乙、戊 3型肝炎感染情况。方法　采用血清流行病学调查方法。结果　学生中抗HAV -IgG ,HBsAg和抗HEV -IgG阳性率分别为 77.0 8% ,1 5 .2 3 % ,1 7.1 5 % ,地区差异明显 ;HAV ,HEV无性别差异 ,而HBV以男性为高 ;农村HAV和HBV感染率均高于城镇 ,而HEV感染率无城乡差别。结论　应该加强对本地区大学生的甲、乙肝预防 ,急需研究出一种有效的戊肝疫