Rapid Diagnosis of Chromosomal Aneuploidy Using a Novel Copy Number Variation Detection Technology

拷贝数变异是近年来发现的新一代遗传标记，其蕴含巨大的信息需要我们去探索和挖掘，但缺乏简便实用的检测技术；染色体非整倍体是临床遗传诊断研究中最重要的一类目标疾病，其发病率高、病症严重且无有效的治疗手段，缺乏快速、准确、简便的诊断方法。本论文旨在开发一种新型的拷贝数变异检测技术，并以染色体非整倍体为对象展开系统研究。 第一章，综述拷贝数变异的发现与相关研究的发展过程，并介绍现有检测方法及其各自的优缺点；综述染色体非整倍体的相关背景、并介绍其传统诊断方法和新颖的分子生物学诊断技术及其各自的优缺点。最后，我们提出了探索一种新型的拷贝数检测技术（连接检测反应依赖的实时定量PCR技术，LDR-qPCR）...Copy number variations (CNVs) recently found are a new generation of genetic markers, containing enormous information desired to explore and mine, but with status quo that there is few simple and practical detection method with high resolution; Chromosomal aneuploidy is a most important class of target-disease in clinical genetic diagnosis. It has high incidence with severe symptom, no effective t...学位：理学硕士院系专业：生命科学学院生物学系_细胞生物学学号：2162007115198

The society assurance of the higher educational quality: basic characteristics, approaches, realizing conditions

政府、社会和高校分别代表着高等教育运行过程中的三种基本力量,也是高等教育质量保障的三大基本主体。相对于政府和高校的质量保障,社会的质量保障主要通过市场经济引导、社会舆论监督、社会中介组织评价等途径来实现。要充分发挥社会力量在高等教育质量保障中的作用,政府的放权与高校的自治是前提,市场的完善与文化的先进是关键,社会力量的整合与引导是手段。Government,society and university are not only the three kinds of basic power in the procedure of higher education operation,but also three basic bodies of higher educational quality assurance.Compared with the quality assurance of government and that of universities.The society assurance is realized by the induction of market economy,the supervision of public opinion and the evaluation of social intermediary organization.In order to fully exert the function of the social participation in the higher educational quality assurance,the premise is the decentralization of the government and the autonomy of universities;the perfection of the market and the advance of the culture is the key;the means is the conformity and conduction of the social forces.湖南省教育科学“十五”规划重点课题(XJK03AG004

ENERGY SAVING TECHNOLOGY STUDY ON EXISTENT DWELLINGS IN NANJING AREA

高校博士点资金资助项目 ,编号 :1999028622;; 江苏省建设委员会和江苏省墙体改革办公室资助项目 ,批文号 :苏建科(1998)298

Isolation and Characterization of an Electricity-producing Strain Shewanella sp. S2 from Marine

以厦门白城海域的潮间带表面沉积物为菌种来源筛选得到一株具有电催化活性的菌株S2,该菌株的16S rrnA和gyrb基因发育树与SHEWAnEllA OnEIdEnSIS Mr-1同支,相似性分别为98.5%和87%,葡萄糖、木糖、半乳糖等碳源利用及最佳生长的nACl浓度与S.OnEIdEnSIS Mr-1有显著差别,因此初步鉴定为SHEWAnEllA属菌株,命名为SHEWAnEllA SP.S2。初步研究了菌株S2产电活性,在以乳酸作为碳源产电时,电压最高为150MV,相应的电流密度为66.1MA/M2。A new electrochemically active bacterium (exoelectrogen),strain S2,was isolated from coastal marine sediments of Xiamen.The phylogenetic trees based on 16S rRNA and gyrB gene sequences showed that strain S2 formed a lineage within the genus Shewanella and had high similarity (98.5% and 87.0%,respectively) with strain S.oneidensis MR-1.The phenotypic characteristics indicated strain S2 could be distinguished easily from S.oneidensis MR-1 by its culture conditions such as pH,NaCl tolerance and carbon source utilization.Based on these results,it is identified as Shewanella sp.S2.The preliminary current generation experiments showed that S2 could use lactate and xylose for electricity production.When lactate was used as a fuel,the MFC had highest voltage (150 mV) and current density (66.1 mA/m2).中国大洋协会项目(No.DYXM-115-02-2-15

Classification Study by Pattern Recognition on the Relationship Between the Trace Elements in Human Hair and Sex

通过对人发样品中22种元素含量的数据进行变量扩维及压缩筛选处理，选出了影响性别判断较显著的变量，用PlS法处理这些变量组成的数据，得到男性与女性分类清晰的二维判别图及预报模型，并根据所建立的预报模型及人发微量元素的含量判别人的性别，准确率为81%.The data of 22 trace elements concentrations in human hair samples were obtained by ICP AES and GFAAS.The variables which have significant influence on discriminating the sex are selected through the treatment of the concentration data by the variable dimension expansion and the variable selection methods.The discrimination plane figure with the good classification is obtained through the treatment of the data with selected variables by PLS method.The prediction models are built and used to distinguish the human sex according to the element concentrations data in human hair.The accuracy of the prediction is 81%.国家教育委员会留学回国人员科研启动费;福建省自然科学基

Simultaneous Determination of Five Catechins and Caffeine in Tea by HPLC and Differentiation of Teas by Cluster Analysis

建立高效液相色谱/二极管阵列检测器(HPLC/DAD)同时测定茶叶中(-)-没食子儿茶素(GC),(-)-表没食子儿茶素(EGC),(-)-表没食子儿茶素没食子酸酯(EGCG),(-)-表儿茶素(EC),(-)-表儿茶素没食子酸酯(ECG),咖啡碱(caffeine)6种组分的分析方法,并采用聚类分析探讨以这6种活性成分为指标对茶叶进行分类的方法。采用C18柱,甲醇和0.05%三氟乙酸水溶液为流动相,梯度洗脱,DAD双波长(210、278 nm)同时检测,采用标准物质保留时间和电喷雾飞行时间质谱(ESI TOF-MS)双重定性。结果表明,各组分的色谱峰均达到基线分离,在210 nm对(-)-没食子儿茶素(GC)定量,278 nm对其它组分定量准确。该法重复性好,灵敏度高,回收率高,已用于不同种类的33种实际茶叶样品的测定。以这6种活性成分的含量为指标,采用聚类分析法可对33个红茶、黑茶、绿茶、乌龙茶样本进行合理分类,并能反映茶叶品质的差异。A method was developed for simultaneous determination of five catechins((-)-gallocatechin,(-)-epigallocatechin,(-)-epigallocatechin-3-gallate,(-)-epicatechin,(-)-epicatechingallate) and caffeine by HPLC with diode array detection(DAD).The tea extract was separated on a C18 column which was then eluted with methanol and 0.05% trifuoroacetic acid solution in gradient elution mode.The chromatograms were simultaneously detected by DAD at λ210 nm for(-)-gallocatechin(GC) and at λ278 nm for the other components.Time of flight mass spectroscopy(TOF-MS) was used for qualitative analysis of real tea sample,the results showed that five catechins and caffeine were baseline separated,indicating that the six components could be determined accurately.The method has been used to determine the catechins and caffeine in 33 tea samples with satisfactory results.Cluster analysis has been used to classify 33 tea samples reasonably and to differentiate the qualities of the tea by using the concentrations of the six components as indices.青岛“2004将才计划”(04-3-JJ-11);; 崂山区崂山茶标准化技术研究(LS-05-KJ1-40

海水中痕量铜的双Sep-Pak柱FIA在线预富集和FAAS检测

采用配有双Sep-Pak柱的流动注射分析仪在线预富集海水样品中痕量铜的8-羟基喹啉络合物,经甲醇洗提后,由火焰原子吸收光谱仪检测,对100ml海水样品的富集倍率可达2个数量级,富集检测速率可为10次/h。多次富集测定的重现性在2.0～5.7％范围内,测定了铜含量在3～7ng/ml浓度范围内的海水样品

日本囊对虾人工诱变子一代遗传变异的RAPD分析

以性腺成熟的日本囊对虾(Marsupenaeus japonicus)作为亲代,进行不同剂量的60Co-γ射线照射.采用RAPD技术对诱变子一代的无节幼体基因组DNA多态性进行了检测.从20个随机寡核苷酸引物共检测177个位点,其中多态位点152个,占85.9%.单个引物获得的标记为6~12个,平均8.85个,分子量在150~2 500 bp之间.遗传相似系数用Ne i的计算方法进行计算,遗传距离则用Lynch的计算方法进行计算.实验数据表明:诱变子代与正常对照组相比,遗传多样性水平较高,诱变产生了较为显著的变异.研究结果证实:在对虾大规模养殖,种质质量严重退化的情况下,人工诱变能够促进基因组更加广泛的变异,为新品种的选育打下坚实的基础;同时证明RAPD技术在检测遗传差异方面具有较高的灵敏度和分辨率

类泛素蛋白及其中文命名

泛素家族包括泛素及类泛素蛋白,约20种成员蛋白.近年来,泛素家族领域取得了迅猛发展,并已与生物学及医学研究的各个领域相互交叉.泛素家族介导的蛋白质降解和细胞自噬机制的发现分别于2004和2016年获得诺贝尔奖.但是,类泛素蛋白并没有统一规范的中文译名. 2018年4月9日在苏州召开的《泛素家族介导的蛋白质降解和细胞自噬》专著的编委会上,部分作者讨论了类泛素蛋白的中文命名问题,并在随后的\"泛素家族、自噬与疾病\"(Ubiquitinfamily,autophagy anddiseases)苏州会议上提出了类泛素蛋白中文翻译草案,此草案在参加该会议的国内学者及海外华人学者间取得了高度共识.冷泉港亚洲\"泛素家族、自噬与疾病\"苏州会议是由美国冷泉港实验室主办、两年一度、面向全球的英文会议.该会议在海内外华人学者中具有广泛影响,因此,参会华人学者的意见具有一定的代表性.本文介绍了10个类别的类泛素蛋白的中文命名,系统总结了它们的结构特点,并比较了参与各种类泛素化修饰的酶和它们的生物学功能.文章由45名从事该领域研究的专家合作撰写,其中包括中国工程院院士1名,相关学者4名,长江学者3名,国家杰出青年科学基金获得者18名和美国知名高校华人教授4名.他们绝大多数是参加编写即将由科学出版社出版的专著《泛素家族介导的蛋白质降解和细胞自噬》的专家

Determination of Lead Content in Standard Materials of Lake Sediment and Tea by ID-ICP-MS

采用2种微波消解体系(HNO3-H2O2和HNO3-H2O2-HF)及3种定量模式(同位素稀释法、标准工作曲线法和标准加入法),对沉积物和茶叶标准物质中的铅进行测定,结果表明:同位素稀释质谱法的各项检测指标显著优于其它两种定量模式(茶叶中铅含量测定值为4.30μg/g),标准加入法次之(3.75μg/g),标准工作曲线法较差(3.61μg/g)。二种微波消解体系中,加HF酸优于不加HF酸,不加HF,茶叶中铅的回收率仅有89%,加入HF则上升为98%。Three different quantitative modes,namely external calibration,standard addition and isotope diluˉtion were compared for the determination of lead contents in lake sediment and tea standard materials with two digestion systems.The results showed that the isotope dilution gave the highest accuracy and precision in comˉparison with the other two modes.But the results were affected by the digestion system.For example,the reˉcoveries of lead content for tea were98%and89%for HNO 3 -H 2 O 2 -HF and HNO 3 -H 2 O 2 digesting sysˉtem,respectively.福建省重大科技项目子课题(2003Y005-04);; 厦门大学现代分析科学教育部重点实验室开放课题(B40402