渤海典型生态灾害的发展变化特征及演变趋势

渤海是中国的内海,受海岸带地区人为活动的影响,海域生态环境退化严重,其主要表现为海洋生态灾害频发。本文对渤海近年典型生态灾害,如赤潮、大型藻类及水母发生的时空分布特征进行了总结,结合水环境变化,对其演变趋势进行了评估,并提出了相应的防控应对建议

Fas ligand and Anti-human DR5 monoclonal antibody induce tumor cell lines apoptosis

目的探讨FasL、Anti-DR5mAb对肿瘤细胞Hela、BGC823、MCF-7、L342、H9101、D6的杀伤作用及机制。方法采用RT-PCR、MTT比色法、电泳、DNA倍体分析、Westernblot等方法。结果H9101、Hela细胞株DR5mRNA水平有表达,D6细胞无表达;H9101、L342细胞株FasmRNA水平有表达,D6细胞无表达。H9101、L342细胞株对FasL、Anti-DR5mAb敏感并呈剂量依赖性;MCF-7、BGC823细胞株对FasL敏感,对Anti-DR5mAb相对敏感。Hela对FasL相对敏感,对Anti-DR5mAb敏感;D6对两种凋亡诱导剂耐受。结论FasL、Anti-DR5mAb能不同程度地诱导肿瘤细胞凋亡,其机制与Fas、DR5、Caspase-8、Bcl-2的表达有关。 【英文摘要】 Objective:To study the cytotoxic effects on tumor cell lines Hela,BGC823,MCF-7,L342,H9101,D6 induced by Fas ligand and anti-human DR5 monoclonal antibodies(Anti-DR5 mAb) and the underlying mechanism.Methods:Fas/DR5 mRNA were detected by RT-PCR. Cytotoxicity exerted by FasL/Anti-DR5 mAb on tumor cell lines was measured by MTT colorimetry and the induced apoptosis was determined by agarose gel electrophoresis.Results:The expression of DR5 on BGC823 and Hela cells were higher and DR5 didn’t express in D6. The ...厦门大学科研启动基金(No.Z03103)资

甲酸甲酯氢解制甲醇铜基催化剂上吸附物种的现场红外光谱表征

应用原位红外光谱的方法，在反应现场条件下，研究了促进型甲酸甲酯（ＭＦ）氢解制甲醇铜铬催化剂上的化学吸附物种．结果表明，在ＭＦ氢解反应条件下，工作态催化剂上主要的化学吸附物种和反应中间物种是ＣＨ３Ｏ－ＣＨＯ…Ｈ－Ｏ（ａ）（１７５１，２７３０，１４５５ｃｍ－１）、ＣＨ３Ｏ－ＣＨＯ…Ｃｕ＋（ａ）（１６５４，２７３０，１４５５ｃｍ－１）、ＣＨ３Ｏ－ＣＨＯ…Ｃｒ３＋（ａ）（１５５２，２７３０，１４４５ｃｍ－１）和ＯＣＨ３（３０１２，２９４６，１４５５ｃｍ－１）．结合对该催化剂活性位本质的探讨，推断了甲酸甲酯氢解制甲醇的主要反应途径

死亡受体5胞外区域的重组、表达及活性鉴定

目的构建死亡受体5(deathreceptor5,DR5)胞外区域(eDR5)的表达载体,表达纯化重组蛋白并鉴定其生物特性。方法通过重叠PCR获得DR5胞外段编码序列,构建pET-22b(+)/DR5表达载体,转化大肠杆菌BL21(DE3),IPTG诱导表达,Ni2+柱亲和纯化,SDS-PAGE、直接ELISA鉴定纯化产物的纯度和特异性,用MTT法检测eDR5蛋白阻断DR5单克隆抗体FMU1.5和TRAIL诱导人胶质瘤细胞株U343(高表达DR5)、U373(低表达DR5)细胞凋亡的作用。结果获得了DR5胞外段编码序列,目的蛋白在上清及包涵体中都有表达,表达量占菌体总蛋白的30%以上,纯化的重组蛋白纯度达95%以上,蛋白产量达9mg/ml。ELISA结果表明所纯化蛋白为eDR5。eDR5蛋白可部分阻断FMU1.5和TRAIL诱导人胶质瘤细胞株U343细胞凋亡的作用,其阻断率与DR5表达相关。结论死亡受体5胞外段基因的成功重组、表达及纯化,为进一步的功能研究奠定了基础

Mechanism of geniposide in improving free fatty acid metabolism in rats with non-alcoholic fatty liver disease

观察栀子苷改善非酒精性脂肪性肝病的效果,从游离脂肪酸探讨栀子苷调节非酒精性脂肪性肝病的作用机制。健康雄性Wistar大鼠40只,随机分为4组:正常组、模型组、栀子苷组和血脂康组,每组大鼠10只,正常组大鼠给予正常大鼠饲料喂养,其余3组大鼠均采用高脂饲料喂养以诱导非酒精性脂肪性肝病,造模时间为8周,从第5周起至第8周末,栀子苷组和血脂康组分别灌服相应的药物。记录大鼠体重、肝湿重、脂肪质量;用相应方法检测肝组织TG,FFA,FAS,AMPK,ACCase及Malonyl-Co A含量,血清CHO,LDL-C的含量,血清AST,ALT的活性;观察肝脏组织肉眼及病理变化(HE染色法)。结果显示,与正常组相比,模型组大鼠的体重;肝湿重;脂肪重;血清CHO,LDL-C,ALT,AST;肝组织TG,FFA,FAS,ACCase及Malonyl-Co A含量皆显著升高(P<0.01);肝组织AMPK活性显著降低(P<0.01),肝组织肉眼外观及病理切片脂肪变性明显,并出现炎症损伤;与模型组相比,栀子苷组大鼠体重、脂肪质量、肝组织FFA含量、血清ALT,AST活性均显著降低(P<0.01),肝湿重,肝组织TG,FAS,ACCase及Malonyl-Co A含量明显降低(P<0.05),肝组织AMPK活性明显增多(P<0.05),肝脏肉眼外观及病理学表现均有所改善;与模型组相比,血脂康组大鼠的肝湿重、脂肪质量、肝组织TG,FFA和血清LDL-C水平明显降低(P<0.05);与血脂康组相比,栀子苷组大鼠的体重、脂肪质量、肝组织FFA含量均显著降低(P<0.01),其他方面无明显差异。结果表明,栀子苷具有显著的改善高脂饮食诱导的大鼠非酒精性脂肪性肝病的药理效应;其改善大鼠非酒精性脂肪性肝病的游离脂肪酸代谢是通过调节"AMPK-ACCase-Malonyl-Co A-FFA"轴来实现的。To observe the effect of geniposide on non-alcoholic fatty liver disease( NAFLD),and discuss the mechanism of geniposide for NAFLD from the aspect of free fatty acid,forty healthy Wistar male rats were randomly divided into normal group,model group,geniposide and Xuezhikang group. The rats in normal group were fed with normal diets,and the rats in other 3 groups were given with high-fat diet for 8 weeks to induce the NAFLD models. From the week 5 to end of week 8,the rats in geniposide and Xuezhikang group were intervened with corresponding medicines. The body weight,liver wet weight,and fat weight of the rats were recorded.Visual and pathological changes in hepatic tissues were observed with HE staining. The contents of TG,FFA,FAS,AMPK,ACCase and Malonyl-Co A in hepatic tissue,contents of CHO and LDL-C in serum and activities of AST and ALT in serum were detected by using corresponding methods. The results showed that the body weight,liver wet weight,and fat weight of the rats,CHO,LDL-C,ALT and AST levels in serum,TG,FFA,FAS,ACCase and Malonyl-Co A levels in hepatic tissues of the rats in model group were significantly higher than those in normal group( P < 0. 01),while AMPK activity was significantly lower than that of the normal group( P < 0. 01),with obvious visual and pathological steatosis in hepatic tissues,and inflammatory injury occurred in model group. Compared with the model group,body weight of the rat,fat weight,levels of FFA in hepatic tissues,ALT and AST activities in serum,liver wet weight,TG,FAS,ACCase and Malonyl-Co A levels were significantly decreased in geniposide group( P < 0. 01),while the AMPK activity in hepatic tissues was significantly increased( P < 0. 05),with improvement in visual and pathological performance.Compared with the model group,liver wet weight,fat weight,TG and FFA levels in hepatic tissues,and LDL-C level in serum were significantly decreased in Xuezhikang group( P < 0. 05). Compared with Xuezhikang group,the body weight of rat,fat weight and FFA level in hepatic tissues were significantly lower in geniposide group( P < 0. 01),but with no significant difference in other aspects. These findings indicated that geniposide was highly effective in improving the pharmacological effect of NAFLD induced by highfat diet,and the mechanism was achieved through AMPK-ACCase-Malonyl-Co A-FFA axis.国家自然科学基金项目(81274155;81503529);; 福建省自然科学基金项目(2014J01374);; 福建省高等学校新世纪人才计划项目;; 厦门市科技计划项目(3502Z20134020);; 福建省中医药科研项目(WZPW201308

Gonad shafting hormone level quantitative analysis and clinical significance for the patients with premenopausal breast disease

目的:研究绝经前(滤泡期或称卵泡前期)的女性乳腺增生和乳腺癌患者HPO轴系激素表达,包括激素雌二醇(E2)、促卵泡激素(FSH)、促黄体激素(LH)、孕酮(P)、睾酮(T)和促催乳素(PRL)6种,为乳腺增生和乳腺癌提供诊断依据。方法:采用放射免疫法检测正常人、乳腺增生和乳腺癌患者各62例血浆E2、FSH、P、T和PRL水平(E2、P和T单位为μg/L,FSH和LH单位为I U/L,PRL单位为g/L),并应用逻辑回归与判别分析等方法进行统计分析。结果:乳腺癌、乳腺增生症和正常人3组人群的激素分布不同,乳腺癌患者的E2(192.59)、FSH水平增高(24.25),LH(30.235 3)与E2、FSH存在协同性增高,Logistic回归分析和聚类分析发现,T水平乳腺癌组低于乳腺增生与正常人群组,差异有统计学意义,P<0.05。P和PRL水平越高,患乳腺癌的危险度上升。结论:通过回归分析和聚类分析,可能提高临床应用性激素检测判别疾病的能力及危险度预测,判别准确率为91.2%。OBJECTIVE: To investigate the HPO shafting including E2,FSH,LH,P,T and PRL in the mammary gland hyperplasia and breast cancer of premenopausal(follicle period or prophase) female patients,and provide bases for mammary gland hyperplasia and breast cancer diagnosis.METHODS: By the radiative immune method,the blood levels of E2,FSH,LH,P,T and PRL(the unit of E2,P and T was μg/L,the unit of FSH and LH was IU/L,and the unit of PRL was g/L) were detected in 62 normal persons,62 patients with mammary gland hyperplasia and 62 patients with breast cancer.Logistic regression analysis and discriminance methods were used for the identification of the results.RESULTS: The hormone distributions in the mammary gland hyperplasia patients,breast cancer patients and normal people were diffrerent.By logical regression analysis and discriminance methods,the levels of E2(192.59) and FSH(24.25) in the breast cancer group increased,while LH(30.235 3) increased cooperativly with the increase of E2 and FSH.The level of T hormone in the breast cancer group was significantly lower than that in the mammary gland hyperplasia group and the normal people group,P<0.05.The higher of P and PRL levels were,the higher the breast cancer risk was.CONCLUSION:The use of logistic regression and distinguish analysis are favorable methods in the diagnosis of mammary gland hyperplasia and breast cancer.The total accuracy is 91.2%.厦门市科技社会发展计划基金(3502Z20044003

Effect of geniposide on the lipid metabolism and inflammatory injury of nonalcoholic fatty liver disease in the hamster

目的:观察栀子苷对高脂饮食诱导的金黄地鼠非酒精性脂肪性肝病模型脂质代谢、肝组织病理变化及炎症损伤的影响,并与栀子进行比较,以明确栀子抗脂肪肝作用的效应成分。方法:24只雄性金黄地鼠按随机数字表法分为正常组、模型组、栀子组和栀子苷组。实验后观察肝组织病理学变化,并检测肝组织Tg含量、血清Tg、CHO含量及AlT、AST活性。结果:与模型组比较,栀子组、栀子苷组肝Tg含量、血清Tg、CHO含量及AlT、AST活性均显著降低,且栀子苷对AlT、AST活性的降低作用优于栀子。结论:栀子苷与栀子对高脂饮食诱导的地鼠脂肪肝均有显著干预作用,且栀子苷抗炎症损伤作用优于栀子。Objective: To observe geniposide's effect on lipid metabolism,hepatic tissue pathological change and inflammatory injury of golden hamsters with nonalcoholic fatty liver disease( NAFLD),caused by high fat diet.Compare the geniposide's effect with gardenia to make clear the effective components in Gardenia that can resist fatty liver.Methods: 24 male golden hamsters were averagely divided into control group,model group,gardenia group and geniposide group.The experiment was based on building the model,and then observe hamsters 'general condition and pathological change of hepatic tissue,examine the content of hepatic tissue TG,serum TG,CHO and the activity of ALT,AST.Results: In gardenia group and gardenoside group,The content of hepatic tissue TG,serum TG,CHO and the activity of ALT,AST are significantly lower than the model group.Besides,gardenoside can lower the activity of ALT,AST better than gardenia.Conclusion: Both gardenoside and gardenia have notable intervention effects on hamsters' NAFLD caused by HFD and gardenoside have better effects on resisting inflammatory injury than gardenia,which suggested that gardenoside was the primary effective component in resisting NAFLD.国家自然科学基金面上项目(81274155); 厦门市重大科技计划项目(3502Z20100006

Jiangzhi and Hepatoprotective effect of salidroside on nonalcoholic fatty liver disease

目的:探讨中药红景天的主要成分红景天苷对高脂饮食诱导的金黄地鼠非酒精性脂肪性肝炎(nASH)的干预作用。方法:18只雄性金黄地鼠随机分成正常组、模型组和红景天苷组,除正常组外,其余两组均给予高脂饮食喂养6周以复制nASH模型;造模第3周起,红景天苷组给予红景天苷药液灌胃4周。6周后处死地鼠,收集标本,检测肝组织甘油三酯(Tg)含量,血清天门冬氨酸氨基转移酶(AST)活性,对肝组织进行HE染色。结果:红景天苷能明显降低肝组织Tg含量及血清AST活性,改善肝组织脂肪变性的病理状态。结论:红景天苷具有显著的抗nASH病理损伤的药理效应。Objective:To explore the intervening effect of the main components of the rhodiola on the nonalcoholic fatty liver disease(NAFLD) for male golden hamsters induced by high-fat diets.Methods:Total 18 male golden hamsters were divided into control group,model group and salidroside group randomly.Male golden hamsters in the model group and salidroside group were fed with high fat diet for 6 weeks to reproduce NAFLD model.After the second week of feeding,male golden hamsters in the salidroside group were given a gavage of salidroside.All male golden hamsters were put to death at the 6 weeks and samples were collected.Hepatic tissue TG contents were detected.Serum AST activities were determined.Hepatic tissues were detected by HE staining.Results:Salidroside decreased hepatic tissue TG contents,serum AST activities and reduced the fatty degeneration in hepatocytes significantly.Conclusion:Salidroside has conspicuous pharmacological effect on the nonalcoholic fatty liver disease in male golden hamsters.国家自然科学基金(No.81274155); 厦门市重大科技计划项目(No.3502Z20100006); 横向课题(No.XDHT2011366A)~

Thermal characteristic analysis of high-power LEDs by structure functions

运用电学法测量功率型lEd冷却瞬态温度曲线,通过数学方法将其转化为积分和微分结构函数来分析器件各区域的热阻和热容,结果发现,各层材料的测量值与理论值基本一致。1μS的瞬态数据采集精度和高的重复性保证了实验结果的准确性和可靠性,运用这种方法比较了3种不同金属芯印刷电路板(MCPCb)对功率型lEd的散热效果,贝格斯Al基板散热性能最好,AnTAl基板次之,普通Al基板最差。研究表明,利用结构函数分析功率型lEd的热特性是一种强有力的方法。The cooling transient temperature curves of high-power LEDs are measured by electritical method.The cumulative and differential structure functions are extracted from these curves to analyse thermal resistances and thermal capacitances of all regimes of high-power LEDs with numeric computational method.It is found out that calculated and measured values of various materials are essentially conformable.The sampling resolution of 1 μs of transient data and high repetition assure the veracity and reliability of experimental result.Subsequently,thermal conduction capabilities of three different metal core printed circuit boards(MCPCBs) with high-power LEDs are compared by this method,and it is discovered that bergquist′s MCPCB has the best thermal conduction capability,ANT′s MCPCB takes second place,and the common MCPCB is the worst.So the structure functions are powerful tools for thermal characteristic analysis of high-power LEDs.国家“863”计划资助项目(2006AA03A175);福建省科技项目(2006H0092;2008J0030);厦门市重大专项资助项目(3502Z20061004

弹道靶上的跨音速实验

本文介绍用弹道靶作跨音速试验的新方法,在二级轻气炮上采用了二个特殊的新技术:充气放炮能发射模型达到亚音速,低充填条件能发射模型达到跨、超音速。由于初始加速度较小,发射的模型外形完整,姿态稳定;加上没有支撑干扰和小洞壁效应的优点,使弹道靶设备在跨音速实验方面有特殊的优越性,用上述技术得到了圆球跨音速流场的清晰照片,其中击波脱体距离、分离点位置、颈部宽度、尾迹形状与超音速、高超音速流动比较有明显变化。文中M≈1.010和M≈0.99的二张全流场照片是十分有意义的。最后提出一个想法,用靶场加压、降温的方法可以提高雷诺数直到10~7以上