Fas ligand and Anti-human DR5 monoclonal antibody induce tumor cell lines apoptosis

Abstract

目的探讨FasL、Anti-DR5mAb对肿瘤细胞Hela、BGC823、MCF-7、L342、H9101、D6的杀伤作用及机制。方法采用RT-PCR、MTT比色法、电泳、DNA倍体分析、Westernblot等方法。结果H9101、Hela细胞株DR5mRNA水平有表达,D6细胞无表达;H9101、L342细胞株FasmRNA水平有表达,D6细胞无表达。H9101、L342细胞株对FasL、Anti-DR5mAb敏感并呈剂量依赖性;MCF-7、BGC823细胞株对FasL敏感,对Anti-DR5mAb相对敏感。Hela对FasL相对敏感,对Anti-DR5mAb敏感;D6对两种凋亡诱导剂耐受。结论FasL、Anti-DR5mAb能不同程度地诱导肿瘤细胞凋亡,其机制与Fas、DR5、Caspase-8、Bcl-2的表达有关。 【英文摘要】 Objective:To study the cytotoxic effects on tumor cell lines Hela,BGC823,MCF-7,L342,H9101,D6 induced by Fas ligand and anti-human DR5 monoclonal antibodies(Anti-DR5 mAb) and the underlying mechanism.Methods:Fas/DR5 mRNA were detected by RT-PCR. Cytotoxicity exerted by FasL/Anti-DR5 mAb on tumor cell lines was measured by MTT colorimetry and the induced apoptosis was determined by agarose gel electrophoresis.Results:The expression of DR5 on BGC823 and Hela cells were higher and DR5 didn’t express in D6. The ...厦门大学科研启动基金(No.Z03103)资