A study about the impact of overpression of Oct4 and Sox2 gene on gastric cancer cell biology and oncology characteristics

目的：通过构建过表达干细胞因子Oct4和Sox2的胃癌SGC7901细胞株，研究过表达干细胞因子Oct4和Sox2对SGC7901胃癌细胞干细胞特性、肿瘤学特性、及生物学特性的影响，并探讨Oct4及Sox2在肿瘤细胞中的作用机制。 方法：1.构建携带Sox2基因的重组质粒，慢病毒载体感染SGC7901-OCT4细胞株得到SGC7901-OCT4-SOX2稳转细胞株。2.利用荧光定量PCR、western–bloting检测过表达Oct4及Sox2的胃癌SGC7901细胞中胃癌干细胞标志基因表达情况。3.细胞增殖和耐药实验、细胞划痕实验、Transwell细胞迁移和侵袭实验、球形克隆形成实验分...Objective: To explore the Stem cell properties and Oncology properties and Biological characteristics of the gastric cancer SGC7901 cell lines by stem cell gene Oct4 and Sox2,and study the mechanism of Oct4 and Sox2 in Tumor cells we construct gastric cancer SGC7901 cell lines into which stem cell gene Oct4 and Sox2 is transfected. Method: 1.Constructed the pHL-hH1-Sox2 and get the SGC7901-OCT...学位：医学硕士院系专业：医学院_外科学学号：2452012115317

The Research on The Problems of Protection of Architectural Works

本文探讨的是建筑作品作者的著作权保护的相关问题。全文共分四个部分。第一部分是有关建筑作品的界定问题。在对有代表性的国际组织、国家和地区建筑作品范围的界定进行分析的基础上，提出自己的看法，即建筑作品应该包括建筑物、建筑设计图和建筑模型。第二部分主要阐述了建筑作品著作人身权与所有权的冲突与协调。建筑作品的人身权具有特殊性，权利人在行使其人身权时，可能发生著作权与所有权的冲突，在这种情况下，著作权通常要让位于所有权。第三部分主要阐述了建筑作品复制权与外观专利权的冲突与协调。建筑作品财产权中的复制权，是一项重要和复杂的权利，本文分析了作者复制权的行使过程中与外观设计专利的冲突表现及原因。在复制权与外观...This article discusses the architectural works author's copyright protection related question .The full text altogether divides four parts. The first part is about the definition of the problem architectural works. it’s based on the representative international organization, national and the local construction work scope definition carries on the analysis in the foundation, proposes author's view,...学位：法学硕士院系专业：法学院法律系_经济法学学号：1202005130044

福建省城市化水平:测量与分析

本文对现有测量城市化水平的几种方法进行评介，并选择单一指标法，用经过调整的人口从业结构指标对福建城市化水平作了测算和初步分析，从而得出了城市化进程的一些规律性结论

Neighboring / Adjacent Coupling Relationship Between Urbanization and Farmland Safety Based on the Decoupling Theory

当前我国快速城市化推进对半城市化、农村等近程区域分布的耕地产生直接、强烈的影响,也对耕地安全造成强大的胁迫效应。为此,构建城市化-耕地安全综合评; 价指标体系,分别计算城市化指数和耕地安全胁迫指数,并基于脱钩理论方法对城市化水平与耕地安全水平的近程耦合关系进行量化分析。以江苏省连云港市为例,; 定量分析20002014年间其城市化水平与耕地安全的脱钩-耦合关系及耕地安全对城市化进程的响应程度。结果表明,; 20002014年间,连云港市城市化指数持续增高,耕地安全胁迫指数波动降低;; 20002007年为强脱钩、弱脱钩、扩张负脱钩及扩张脱钩的波动状态;; 20082014年呈更加强烈的波动状态,包括20082012年的强脱钩和2013年的扩张负脱钩状态,这反映出区域发展规划、土地利用规划和城市总体; 规划的实施对这一典型近程耦合系统产生了较大影响。研究期内连云港市城市化水平大幅提高,耕地安全所受胁迫程度不断降低,两者虽总体呈强脱钩关系,但仍需; 注意近程耦合结果波动性较大所揭示的不确定性问题,需要谨慎权衡城市化相关政策对耕地安全的影响。与过去的宏观、单一指标模型相比,基于近程耦合关系提出; 的多指标脱钩-耦合模型更具综合性,能更加全面、精细化分析快速城市化进程与耕地安全保护之间的复杂关系,为半城市化地区景观安全格局评估提供支撑,为城; 市化与耕地保护之间的协调发展提供决策支持。The current rapid development of urbanization in China has directly and; seriously affected farmlands distribution in its para-urbanized; neighboring /adjacent regions,and also posed severe stresses on safety; of these land. It is,therefore,essential to build up a comprehensive; urbanization and farmland safety assessment indicator system,work out; quantitatively urbanization index and farmland safety stress index,and; analyze quantitatively neighboring coupling relations between; urbanization and arable land safety based on the decoupling theory and; method. A case study was conducted of Lianyungang City of Jiangsu; Province to explore quantitatively coupling-decoupling relationship; between urbanization and farmland safety and response of farmland safety; to the progress of urbanization during the period from 2000 to 2014.; Results show that during the study period,urbanization index of; Lianyungang City increased steadily,while farmland safety stress index; fluctuated with a declining trend. Their relationship displayed a wavy; trend from 2000 to 2007,i.e. strong decoupling-weak decoupling-expanding; negative decoupling-expanding connection and a strong fluctuation curve; from 2008 to 2014,including strong decoupling during 2008-2012 and; expanding negative decoupling in 2013,which reflects strong impacts of; the implementation of the regional development,land use and city overall; programs on this typical neighboring / adjacent coupling system. During; the study period,Lianyungang City made a huge and steady progress in; urbanization, while reducing fluctuation of its stress on farmland; safety. Though the two posed a strong decoupling relationship,the; problem of uncertainty reflected in the huge fluctuation of the result; of the neighboring /adjacent decoupling calls for high attention,and the; influence urbanization had on farmland safety needs to be weighed; prudently. Compared to the macroscopic single indicator models used in; the past,the multi-index decoupling-coupling model built for the; neighboring coupling relationship is more comprehensive and capable of; analyzing the complex relationship between rapid urbanization and; protection of farmland safety in a more holistic and finer way.; Furthermore,it may provide theoretical support to landscape safety; assessment of para-urbanizd regions and to decision-making on harmonized; development of both urbanization and protection of farmland.国家自然科学基金; 厦门大学大学生创新创业训练计划; 中国科学院大学生创新实践训练计

1976 Vol. 24 Number 11

https://engagedscholarship.csuohio.edu/lawpublications_gavel1970s/1100/thumbnail.jp

一种用于研究激光等离子体反应的质谱仪

本文介绍了一种自行设计与研制的可用于研究激光等离子体反应的质谱仪。它以高能量密度的脉冲激光作用于固相的反应体系,产生高温等离子体,以两台飞行时间质谱计同时记录激光等离子体反应产生的正负离子质谱。作为反应研究的实例,本文还介绍并讨论了硫、磷与银、铌等简单二元体系的反应结果

Design of Ultra-wideband Printed Rectangular Monopole Antenna

针对超宽带通信应用,研究影响印制单极天线阻抗带宽的主要因素,设计基于微带馈电的小型化印制矩形单极天线。按照等效性原理,采用黄金分割比设计矩形振子体;通过接地面上端引入渐变梯形或凹形结构,同时调整馈入端接地面间隙,可实现印制矩形单极天线的超宽带特性。对具有渐变梯形或凹形接地面结构的微带馈电矩形印制单极天线结构进行优化,仿真结果表明,前者的阻抗带宽为2.96~17.94gHz,后者的阻抗带宽为2.9~13.3gHz,而两者的辐射方向基本保持不变。实测结果与仿真结果基本一致,达到了超宽带通信应用的要求。To achieve ultra-wideband(UWB)radio applications,the microstrip-fed printed rectangular monopole antenna is analyzed and devised.Using golden section ratio the rectangular monopole is of layout based on equivalent principle.To maximize the impedance bandwidth,it is necessary introducing the taper or concave ground plane and feed gap of microstrip line.The simulated results of the optimized-size antennas show that the impedance bandwidth defined by return loss less than-10dB is from 2.96GHz to 17.94GHz with a ratio of about 6.06∶1 for taper ground antenna and from 2.9GHz to 13.3GHz with a ratio of about 4.59∶1 for concave ground antenna.At the same time a nearly omnidirectional radiation pattern is exhibited.These results are confirmed by the experimental test and these compact size antennas are suitable for various wideband applications.福建省科技计划重点资助项目(2007H0036

河蚬乙酸乙酯提取物的抗氧化活性及其对人胆管癌细胞的抑制作用

作为传统中药的河蚬具有退热、治疗肝病和解酒等功效.以新鲜河蚬(Corbicula fluminea)肉为原料,经甲醇抽提,乙酸乙酯萃取等方法得到河蚬乙酸乙酯提取物.运用DPPH法检测其清除自由基的能力,通过MTT法和倒置显微镜观察其对人胆管癌细胞QBC939生长的影响,并通过流式细胞术分析其对QBC939细胞周期的影响.结果显示:河蚬乙酸乙酯提取物对DPPH自由基具有一定的清除作用,在较低的浓度下有较高的清除效果,IC50值为0.6 mg/mL,且随其浓度的增加而不断增强;河蚬乙酸乙酯提取物对QBC939细胞有显著的抑制作用,处理48 h,IC50值为250μg/mL,细胞形态发生明显变化;处理后的QBC939细胞出现明显的凋亡峰.研究表明:河蚬乙酸乙酯提取物不但具有抗氧化活性,且对体外培养的QBC939细胞具有明显的增殖抑制作用,能够改变QBC939细胞周期分布,并诱导QBC939细胞发生凋亡.说明河蚬乙酸乙酯提取物可能具有天然药物开发的潜力,同时也为胆管癌的防治提供新的思路和方法

STUDIES ON LASER PLASMA REACTION OF SILVER AND SULFUR

It is well known that under the ordinary conditions the product of the reaction of silver and sulphur is Ag_2S. However, it seems to be an interesting attempt to carry out such a reaction under extreme conditions, for example, in the plasma, the reaction is carried out only between silver and sulfur in no solvent. Since there is quite a part of reactants in the excite

激光等离子体质谱用于岩样化学成份的分析

在自制的仪器上,记录了一系列岩样的激光等离子体质谱。通过对正负离子质谱的分析,可以确定岩样的化学组成。本文通过对仪器工作原理、实验条件和测试结果的介绍,显示出该测试手段具有分析速度快、分析结果准确可靠、所得信息较为丰富、可进行微区与深度分析等优点。文章还讨论了不足之处及改进方向