秋海棠化学成分的研究

从秋海棠中分得６个化合物，经ＴＬＣ、熔点测定、１ＨＮＭＲ和１３ＣＮＭＲ鉴定为β－谷甾醇、β－香树素、胡萝卜甙、豆甾醇、豆甾醇－３－β－Ｄ－吡喃葡萄糖甙和４′，５，７－三羟基酮－６－Ｏ－β－Ｄ－吡喃葡萄糖甙，均为首次从该植物得到

浆细胞样树突状细胞在宿主抵御隐球菌肺部感染中的作用及机制研究进展

肺隐球菌病是由隐球菌感染引起的常见真菌病,由于症状的非特异性,临床上诊断较为困难。作为条件致病性真菌感染,肺隐球菌病的结局主要与宿主免疫力有关。目前肺隐球菌病免疫学发病机制研究主要局限在T细胞和巨噬细胞。近年研究表明,作为树突状细胞亚群之一的浆细胞样树突细胞,由于其激活后可以产生大量的I型干扰素并活化相关的T细胞,所以在机体抵抗病毒和细菌免疫中发挥着重要的作用。但是浆细胞样树突细胞在真菌病,尤其是在隐球菌病的发生发展中发挥的作用尚不明确。本文将介绍肺隐球菌病的临床表现、诊治及T细胞和巨噬细胞在肺隐球菌病中的免疫机制,并通过介绍肺隐球菌病和浆细胞样树突细胞及二者之间已有报道的联系,初步阐述浆细胞样树突细胞在肺隐球菌病免疫学发病机制中的相关作用。国家自然科学基金(31770161);;上海市科学技术委员会基金(14DZ2272900);;国家卫生部基金(2018ZX10101003);;上海市科委技术标准专项(16DZ0500401)~

Identification of Irradiated Protein-rich Food by Dissociative Tyrosine

建立了高效液相色谱-游离酪氨酸法鉴定高蛋白含量辐照食品的方法。向经过辐照的高蛋白含量样品中加入0.1 MOl/l HCl,使细胞裂解并释放出游离酪氨酸,离心除去细胞碎片后,向上清液中加入80%丙酮并在-20℃下冰冻沉淀除去大部分蛋白,样品中的酪氨酸与三氟乙酸形成离子对后经C18色谱柱分离。在100~1 500μg/l质量浓度范围内,邻酪氨酸和间酪氨酸均具有良好线性,方法的回收率为61%~116%,定量下限(S/n≥10)为10μg/l。该法避免了蛋白质的水解,具有操作简便、测定准确等优点,能很好地区分未经辐照与经5 kgy以上剂量辐照的食品。A novel method was presented for identification of irradiated protein-rich food by HPLC with dissociative tyrosine.The procedure involved the homogenization of fresh sample with 0.1 mol/L HCl,followed by centrifugation to remove the cell debris and the solvent precipitation of protein with acetone at-20 ℃,then the tyrosine was separated by forming ion pair with trifluoroacetic acid on a C18 column.The average recoveries of the drugs ranged from 61% to 116% at the spiked levels of 100-800 μg/L.The method is simple and double-quick,and could be efficiently identified food sample irradiated at a dose of 5 kGy or above.国家质量监督检验检疫总局科研计划资助项目(2009IK183

First-Principles Study of Effect of Strain on the Band Structure of ZnO Monolayer

采用基于密度泛函理论的第一性原理计算对单层znO薄膜能带结构的应变调控进行了研究.计算结果表明:沿着之字形方向的压缩应变和扶椅形方向的拉伸应变对薄膜带隙的调控都是线性的,而且带隙调控的范围最大;相反地,在沿着之字形方向的拉伸应变和扶椅形方向的压缩应变的调控下,带隙则呈现出非线性的变化.对于双轴应变的拉伸与压缩,带隙的变化都是非线性的.这种通过不同的应变加载方式来实现对带隙不同程度的调控,对znO薄膜在光学和催化等领域的应用具有重要的指导意义.The effect of strain on the band structure of the ZnO monolayer has been investigated by firstprinciples calculations based on density functional theory.The results reveal that the band structure of the ZnO monolayer presents different dependences on three types of strain.The band gap linearly and steeply varies under uniaxial zigzag compressive strain and armchair tensile strain, while it shows nonlinear dependence on the other types of strain.Therefore, uniaxial zigzag compressive strain and armchair tensile strain should be the most effective to tune the band gap.This work has significant implications for application of strain to tune the optical and catalytic properties of ZnO nanofilms.国家自然科学基金(10702056;11204252); 四川省教育厅(12ZA072)资助项目~

Determination of fungicide anilinopyrimidine residues in food by series solid phase extraction-high performance liquid chromatography-tandem mass spectrometry

建立了食品中嘧啶胺类杀菌剂嘧霉胺、嘧菌胺及嘧菌环胺残留的串联固相萃取-液相色谱-串联质谱(HPlC-MS/MS)检测方法。胡萝卜、辣椒等样品经乙酸乙酯提取,石墨化炭黑-弗罗里硅藻土串联固相萃取柱(EnVI-CArb-flOrISIl SPE)净化后,在HPlC-MS/MS仪上进行检测分析,采用外标法定量。质谱分析采用电喷雾电离,正离子扫描,多反应监测模式。结果表明,柱净化后无明显的基质效应,嘧霉胺、嘧菌胺和嘧菌环胺在1~20μg/l内相关系数可达0.999 0以上,具有良好的线性关系;每种杀菌剂选择两个离子对,其中一组用于定量:嘧霉胺M/z 200.1/107.1,嘧菌胺M/z 224.0/106.0及嘧菌环胺M/z 226.0/108.1;另一组用于确证:嘧霉胺M/z 200.1/183.1,嘧菌胺M/z 224.0/131.1和嘧菌环胺M/z 226.0/133.1。样品中添加0.1、0.5、1.0μg/kg的标准品,其回收率为73.2%~98.7%,相对标准偏差(n=10)小于10%;嘧霉胺、嘧菌胺、嘧菌环胺的检出限(信噪比(S/n)=3)均为0.03μg/kg;嘧霉胺、嘧菌胺、嘧菌环胺的定量限(S/n=10)均为0.1μg/kg。实验结果表明,该方法提取效果好,具有良好的灵敏度、回收率和重复性。A high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS) method was established for the determination of fungicide anilinopyrimidine residues including pyrimethanil,mepanipyrim,and cyprodinil in foodstuffs with series solid phase extraction(SPE).The food sample was first extracted with ethyl acetate,and then purified by an ENVI-Carb cartridge and a Florisil SPE cartridge.The analytes were determined by HPLC-MS/MS and quantified by external standard method.The mass spectrometric detection was operated with electrospray in positive ionization mode and the fungicide anilinopyrimidines were identified in multiple reaction monitoring(MRM) mode.The linear range of the method was 1-20 μg/L,with the correlation coefficient(r2) over 0.9990.The transitions of the precursor ions to two selected product ions were involved,in which one group for quantification were m/z 200.1/107.1 for pyrimethanil,m/z 224.0/106.0 for mepanipyrim and m/z 226.0/108.1 for cyprodinil,and the other group for identification were m/z 200.1/183.1 for pyrimethanil,m/z 224.0/131.1 for mepanipyrim and m/z 226.0/133.1 for cyprodinil,respectively.No significant matrix effect was found for spiked samples after the purification.The recoveries of the fungicide anilinopyrimidines spiked in food samples were 73.2%-98.7% at the spiked levels of 0.1,0.5,1.0 μg/kg.The relative standard deviations(RSDs) were less than 10%.The limits of detection(LODs) and limits of quantification(LOQs) were 0.03 μg/kg and 0.1 μg/kg for the three fungicides,respectively.The results showed that the proposed method is sensitive and accurate for the determination of fungicide anilinopyrimidines in foodstuffs.The method should be applicable for routine analysis of pyrimethanil,mepanipyrim,cyprodinil residues in foodstuffs.厦门市科技计划项目(3502Z20092009、3502Z20102013);国家质检总局科技计划项目(2010IK192

Effects of Low Energy Carbon Ion and Nitrogen Ion Implantation on Germinant Rate, Growth Weight and Peroxidase of Stevia rebaudiana Bertoni

以经过碳、氮 (75× 1 0 14 ke V/ cm2 )离子注入处理后的甜叶菊 (Stevia rebaudianaBertoni)种子为材料 ,研究低能离子注入对甜叶菊萌发率、生长量及过氧化物同工酶的影响。结果表明 :(1 )萌发期处理组的萌发率低于对照 ,萌发延迟 ,幼苗成活率不及对照组 ;萌发后期处理组的相对生长量、过氧化物酶活性高于对照组。 (2 )幼苗移至实验地种植后 ,处理组幼苗长势明显优于未处理组 ,处理组 60 d蕾株比明显高于对照组。 (3)过氧化物酶同工酶谱分析发现 ,对照组具有完整 6条基本带的植株数为 1 2 ,具有完整 B1、B2基本带的植株数为 2 0 ;C+处理组分别为 5和 1 1 ,N+处理组分别为 5和 6。统计发现低能离子的注入影响最大的是同工酶谱 B1、B2带。 N+处理组生物学负效应大于 C+处理组The paper reports the effects of low energy Carbon and Nitrogen ion (75×10 14 keV/cm 2 )implantation into S. rebaudiana seeds.on germinant rate, relative growth weight and peroxidase of S. rebaudiana. The results showed: (1)The seed germinant rates were lower and seeding servival rates and deve lopment speeds were slower in the treated groups than those in the control in the initial period of seed germinating;but in the late period of seed germinating the relative growth weight and peroxidase activity were higher in treated groups than those in the control.(2)When seedings were transplanted into experimental field the growth and development were speeded up in treated groups than that in the control.The rate of flower bud plants were higher in treated groups than that in the control. (3)The isozymogram of peroxidase by PAGE showed that there were 12 plants which had 1～6 basic bands and 20 plants which had B1,B2 bands in the control,but only there were 5 plants and 11 plants, respectively, in C + treated groups,and there were 5 plants and 6 plants, respectively, in N + treated groups. The result also showed there were more negative effects by ion implantation into S. rebaudiana in N + treated groups than those in C + treated groups in the periods of seed germinating and plant growth.国家基金委重大项目资助课题!( 1 9890 30 0

盐封后碳化钼电催化剂的制备及其氢析出性能（英文）

碳化钼具有低廉的价格、优越的催化性能以及良好的稳定性而被人们认为是极好的可以替代Pt等贵金属的氢析出反应（HER）催化剂。本工作采用钼酸钠和2,6-二氨基吡啶为反应原料,之后不断进行...supported by the National Key Research and Development Program of China(2017YFA0206500);; the National Natural Science Foundation of China(21773198,U1705253)~

广东省糖尿病专科护士研究生课程进修班教育的实施

2006-2007 > Academic research: refereed > Publication in refereed journalVersion of RecordPublishe

Analysis of the Content of Stevia Sweeteners by Capillary Zone Electrophoresis

本文介绍了一种用毛细管区带电泳法筛选甜菊糖苷突变体的有效方法。根据实验结果 ,优化的电泳条件为 :60mmol/LTris 硼酸缓冲液 (pH 8.0 ) ,柱温 30℃ ,工作电压 2 5kV。优化条件下 ,甜菊苷 (Ste vioside)迁移时间的R .S .D为 0 .45% (1 5次 ) ,且在 7.45× 1 0 - 5～ 1 .74× 1 0 - 2 mol/L的浓度范围内存在良好的线性关系 (r=0 .9994) ,甜菊主要糖苷在 5min内均可实现分离。在优化条件下 ,本实验研究了低能离子注入后甜菊主要糖苷含量变化 ,结果令人满意。This paper introduced an effect capillary zone electrophoresis method for screening of stevia mutation. According to the experimental results, the optimum separation conditions were selected as: 60 mmol/L Tris tetraborate buffer of pH 8.0, 30 ℃ and 25 KV. Under optimum conditions, the R.S.D. of stevioside migration time for 15 runs was 0.45%. The detector response for stevioside was linear over the range of 7.45×10 -5 ～1.74×10 -2 mol/L (r=0.9994). Stevia main sweeteners cound be separated effectively in less than five minutes. This method was applied for determining the variation of stevia main sweeteners after low_energy ions implantation

Determination of five poisonous elements in holothurian sold on the market by Microwave Digestion with ICP—MS

建立了微波消解—电感耦合等离子体质谱法测定海参中Cu,AS,Cd,Hg,Pb 5种有毒元素含量的方法,采用In元素为内标,利用其回收率对以上5种元素的测定结果进行校正,该方法变异系数(CV%)为0.2%--4.0%,In元素加标回收率为96.7%--104.5%,说明该方法灵敏度高,结果准确可信。利用该方法对10批不同市售海参中5种有毒元素含量进行了测定,结果表明10批市售海参中的AS含量均超标,部分批次海参中的Cd、Pb超标,说明市售海参确实存在有毒元素超标的问题。A method based on microwave sample digestion and inductively coupled plasma-mass spectrometry(ICP—MS) detection was established for determination of Cu,As,Cd,Hg and Pb in holothurian sold on the market.Indium was used as internal standard element to calibrate the results of determination of these five elements.Determination limits of these five elements were in the range of 0.006～0.063 μg/g,coefficient of variation(CV%)were in the range of 0.2%～4.0%,and the recovery of In was in the range of 96.7%～104.5%.The method has been applied to determine the five poisonous elements in ten holothurian samples sold on the market.The results indicated that the over-standard problem of poisonous elements in holothurian sold on the market existed.The content of total arsenic was over standard in all the ten samples,but the over standard problem of lead and cadmium was only found in part of the samples.国家自然科学基金(20675021);中国近海海洋综合调查与评价908专项(908-02-05-04)资