A NEW SPECIES OF CARASSOTREMA PARK, 1938 (DIGENEA: WARETREMATIDAE)

鲫吸虫 ,新种Carassotremaclupanodonaesp .nov.检获于福建省厦门海域斑Clupanodonpunctatus (TemmincketSchlegel)的消化道。新种同该属已知种中的孟加拉鲫吸虫CarassotremabengalenseRekharani&Madhavi,1 985最为相似 ,但可以与后者相区别的地方在于肠叉位于腹吸盘后部而非腹吸盘前部 ;肠管止于睾丸后缘水平而非止于近体末端 ;睾丸边缘光滑而非不规则The paper deals with a new species of Carassotrema Park, 1938 obtained from the intestine of Clupanodon punctatus (Temminck et Schlegel). Type specimens are deposited in Parasitology Research Laboratory, School of Life Sciences, Xiamen University. Measurements are given in millimeters, as the ranges followed by the mean in parentheses, unless otherwise stated.福建省自然科学基金 (B0 0 10 0 0 2 )资助;; 福建省青年科技人才创新项目资

TWO NEW RECORDS OF GENERA, WITH REDESCRIPTION OF ACCACOELIUM CONTORTUM(RULDOLPHI, 1819) AND ACCACLADOCOELIUM PETASIPORUM(ODHNER, 1928)(DIGENEA: ACCACOELIIDAE)

在中国南海的矛尾翻车鲀(Masturus lanceolatus)中发现了两种复殖吸虫,Accacoelium contortum(Ruldolphi,1819)和Accacladocoelium petasiporum(Odhner,1928),它们隶属于凸腹科(Accacoeliidae Odhner,1911),这两种复殖吸虫的子宫走向和以前的描述不同,对它们的形态进行了重新描述。另外,研究中的A.contortum是在宿主肠道中发现,而不是如从前记录的发现在宿主的鳃上。这两种虫及其所在的属都是首次在中国发现和报道。Two species of the Accacoeliidae Odhner, 1911, Accacoelium contortum(Ruldolphi, 1819) and Accacladocoelium petasiporum(Odhner, 1928), are redescribed based on new materials collected from the intestine of the sharptail mola Masturus lanceolatus(Lienard) off the coast of Hainan Island in the South China Sea. Characteristics of uterus of the two species are different from previous descriptions, and the specimens of A. contortum were collected in intestine rather than gills as reported previously. Both of these two species and their genera are firstly recorded in China.National Science Foundation of China(No.:31072215

Population ecology of Ligophorus hamulosus on Liza carinatus

棱鮻(lIzA CArInATuS)样品于2000年12月--2001年10月取自广东湛江。按常规方法从鱼鳃上挑取小钩鲻鱼虫(lIgOPHOruS HAMulOSuS),进行压片、计数。结果显示:随着宿主体长的增长,小钩鲻鱼虫种群的平均密度、平均感染强度呈上升趋势;从12月到次年4月,其平均密度和平均感染强度上升,随后呈下降趋势,8月最低,10月略有回升;小钩鲻鱼虫种群在不同大小的宿主上和在不同季节均呈聚集分布。Ligophorus hamulosus was collected from the gills of Liza carinatus,which were captured in Zhanjiang,Guangdong Province from Dec,2000 to Nov.2001.The routine Parasitology methods were adopted in the study.The results showed that the abundance and the mean intensity of L.hamulosus population were increased with the increase of body length.From Dec.towards next Apr.the abundance and the mean intensity was increased,and afterwards they declined the lowest in Aug.but in Oct.they were increased slightly.The prevalence seemed to be decreased with the increase of the mean water temperature.The frequency distribution and the population parameters of L.hamulosus showed that the parasite populations in the host population group were over-dispersed for different body length and in different seasons.国家自然科学基金(39870144);广东省自然科学基金(980314

Effect of Formalin Treatment on the Monogenean Pseudorhabdosynochus epinepheli (Monogenea) and the Fish Epinephelus coioides

20 0 0年 4月研究了斜带石斑 Epinepheluscoioides寄生拟合片盘虫Pseudorhabdosynochusepinepheli的防治方法。海水福尔马林对拟合盘虫的作用表现为 :2 5min处理的半致死浓度为 2 5 0× 10 - 6 ,5 0min处理的半致死浓度为 5 0× 10 - 6 。淡水福尔马林的效果为 :3min的半致死浓度为 83× 10 - 6 ,纯淡水处理的半致死时间为 2 1min。海水福尔马林对斜带石斑的毒性表现为 :30min和 4 5min的半致死浓度分别为 94 0× 10 - 6 和 6 4 5× 10 - 6 。淡水福尔马林对斜带石斑的毒性表现为 :5min和 10min的半致死浓度分别为 130 0× 10 - 6 和 5 0 0× 10 - 6 。福尔马林处理海水中的斜带石斑半致死浓度介于 6 0 0× 10 - 6 ～ 10 0 0× 10 - 6 ,淡水处理对斜带石斑半致死浓度介于 95× 10 - 6 ～ 115×10 - 6 。淡水中斜带石斑的半麻醉和半致死时间分别为 95min和 115min。以上结果表明 :海水和淡水福尔马林对石斑拟合片盘虫的致死浓度 ,处于斜带石斑的安全范围之内。Prophylactic methods against Pseudorhabdosynochus epinepheli (Monogenea) infection on the fish Epinephelus coioides were investigated in April 2000.The effect of formalin in seawater on P epinepheli was that the semi_lethal concentration is 250×10 -6 for 25 min,and 50×10 -6 for 50 min.The effect of formalin in freshwater on P epinepheli is reported as:the semi_lethal concentration was 83×10 -6 for 3 min.The semi_lethal time for freshwater alone was 21 min.The toxicity of formalin in seawater to E coioides was assessed:the semi_anaesthetic concentration was 940×10 -6 in 30 min and 645×10 -6 in 45 min.The toxicity of formalin in freshwater to E coioides was as follows:the semi_anaesthetic concentration was 1 300×10 -6 in 5 min and 500×10 -6 in 10 min.The semi_lethal formalin concentration to E coioides in seawater was between 600×10 -6 and 1 000×10 -6 ,and in freshwater was between 250×10 -6 and 500×10 -6 .The semi_anaesthetic and semi_lethal time of E coioides in freshwater were 95 min and 115 min respectively.These results demonstrated that the lethal formalin concentrations for P epinepheli in both seawater and freshwater fell in a safe range for E.coioides.国家 8 6 3资助项目 (2 0 0 1AA6 2 10 10 ) ;; 广东省自然科学基金资助项目 (0 2 16 81

A New Genus Record of Phyllobothriidae from Marine Fish Dasyatis akajei in China

报道检获于厦门海域赤魟(Dasyatis akajei)肠道中的四叶目叶槽科蔷薇属叶枕蔷薇线虫Rhodobothriumpulvinatum Linton 1889,经鉴定比较,为我国鱼类绦虫属的新记录。本次共剖检赤魟46尾,阳性2尾,感染率为4.3％,感染强度为5～7条/尾,并对所获虫体进行了描述。Rhodobothrium pulvinatum, one species of Phyllobothriidae (Cestoda: Tetraphyllidea) from marine fish Dasyatis akajei in Xiamen, Fujian, China was reported. The genus Rhodobothrium Linton, 1889 was erected for R. pulvinatum from the intestine of Dasyatis kuhli. which was new genus record in China, Dasyatis akajei was also a new record of host. 46 of Dasyatis akajei were examined, 2 of which were infected with cestode, the infective rate was 4. 3%, the infective intensity of the cestode was 5-7, the description of the worm is provided in the present paper.厦门大学校级自选课题资助(2002

胸腺素原体内抑制小鼠肝癌细胞H22的研究

根据PArkIn等[1]报道,2000年全球新发癌症病例为1 010万例,死亡620万。在fErlAy等[2]报道中最常见的癌症中致死率排列第三是肝癌。在我国肝癌的死亡率占全部恶性肿瘤死亡的18.8%,仅次[3]厦门市科技项目(3502Z20083004);国家“973”项目(2007CB513103);福建省医药卫生创新项目(2009-CXB-51)资

Study on the prothymosin α as vaccine adjuvant of P.yoelii

目的:研究胸腺素α原(PrOTHyMOSInα,PrOTα)作为约氏疟原虫疫苗免疫佐剂的作用。方法:提取P.yOElII-17Xnl全蛋白作为抗原,用胸腺素α原作为免疫佐剂,免疫小鼠。具体方案为:昆明小鼠分为4组,每组6只,A组免疫P.yOElII-17Xnl全蛋白+PrOTα;b组免疫P.yOElII-17Xnl全蛋白;C组只注射PrOTα;d组为空白对照,以相同体积的生理盐水代替。免疫结束后感染致死的P.yOElII-17Xl,1x107个虫/只小鼠。结果:感染后的前10天A组小鼠疟原虫血症平均值要低于其他三组,且最终有3只小鼠存活下来,存活率50%,C组有一只小鼠存活,b、d组小鼠全部死亡。结论:用P.yOElII-17Xnl全蛋白做抗原,用PrOTα作为佐剂,比单独用P.yOElII-17Xnl全蛋白对小鼠有更好的免疫保护作用,提示了PrOTα可以成为一种有潜力的蛋白疫苗。Objective:To investigate the function of prothymosin α(ProTα) as vaccine adjuvant of P.yoelii.Methods:The mice were immunized with the total protein extracted from P.yoelii-17XNL as antigen,together with prothymosin α as adjuvant.Programs:Kunming mice were divided into A,B,C and D group.A group was immunized with P.yoelii-17XNL total protein and ProTα;B group was immunized with P.yoelii-17XNL;C group was only injected with ProTα;D group was the control,only injected with physiological saline.And then,the mice of each group was infected with P.yoelii-17XL,the dosage was 1×107/mice.Results:The parasitemia of A group-mice was lower than the other three groups in the first 10 days after infection,and eventually there were three mice survived in A group,the survival rate was 50%,one mouse survived in C group,all of mice in B group and D group died.Conclusion:Mice immunized with P.yoelii-17XNL total protein as antigen together with ProTα as adjuvants,had better immune protection than those immunized with P.yoelii-17XNL protein only.The present results suggest that the ProTα can act as a potential adjuvant in protein vaccine.厦门市科技项目(3502Z20083004);国家“973”项目(2007CB513103)资

Characterization and immunoprotective effect of SjIrV1,a 66 kDa calcium-binding protein from Schistosoma japonicum

钙结合蛋白是日本血吸虫生长发育不可缺少的蛋白,具有非常广泛而重要的功能。在课题组日本血吸虫体被表膜蛋白研究基础上,利用PCr技术克隆了中国大陆株日本血吸虫66 kdA钙结合蛋白(SJIrV1)编码基因的CdnA序列,blAST分析与菲律宾株日本血吸虫SJIrV1 CdnA编码序列一致,荧光定量PCr分析表明该基因在童虫和成虫期不同发育阶段均有表达,其中在35 d和42 d成虫中表达量较高,在42 d雌虫中该基因表达水平远高于42 d雄虫。构建重组表达质粒PET28A(+)-SJIrV1,在大肠杆菌中成功诱导表达,重组蛋白主要以可溶性形式存在,通过高效液相色谱法(rP-HPlC)以及串联质谱法(MS/MS)鉴定所获蛋白为目的蛋白SJIrV1。蛋白质印迹(WESTErn blOTTIng)分析结果显示重组蛋白能被感染日本血吸虫鼠血清和免疫鼠血清所识别,SJIrV1蛋白在虫体各发育阶段中均表达。免疫荧光染色实验观察表明SJIrV1主要分布在日本血吸虫成虫的表膜。应用重组蛋白免疫bAlb/C小鼠后,免疫鼠血清中检测到较高水平的特异性Igg、Igg1和Igg2A抗体。结果表明SJIrV1可能在日本血吸虫的生长发育过程中起着重要作用。Calcium-binding protein is an indispensable protein which performs extensive and important functions in the growth of Schistosoma japonicum.Based on our primary study on tegument surface proteins of S.japonicun,a cDNA encoding a 66 kDa calcium-binding protein of S.japonicum(Chinese strain) was cloned,sequence analysis revealed that it was identical with that of SjIrV1 of Philippines strains S.japonicum.The expression of SjIrV1 were detected by Real-time PCR,using cDNA templates isolated from 7,14,21,28,35 and 42 days worms and the results revealed that the gene was expressed in all investigated stages,and the mRNA level of SjIrV1 is much higher in 42 d female worms than that in 42 d male worms.The cDNA containing the open reading frame of IrV1 was subcloned into a pET28a(+) vector and transformed into competent Escherichia coli BL21 for expression.The recombinant protein was purified using a Ni-NTA purification system,and confirmed by high performance liquid chromatography(RP-HPLC) and tandem mass spectrometry(MS/MS).Western blotting analysis showed that recombinant SjIrV1(rSjIrV1) could be recognized by the S.japonicum infected mouse serum and the mouse serum specific to rSjIrV1,respectively.Immunofluorescence observation exhibited that SjIrV1 was mainly distributed on the tegument of the 35-day adult worms.ELISA test revealed that IgG,IgG1 and IgG2a antibodies are significantly increased in the serum of rSjIrV1 vaccinated mice.The study suggested that rSjIrV1 might play an important role in the development of S.japonicum.国家自然科学基金(No.31172315); 上海科技发展基金(No.12140902700); 中国博士后科学基金(No.2012M510630)资助~

胸腺素原体内抑制小鼠肝癌细胞H22 的研究

作者简介: 蔡报伟( 987年－ ) ，男，在读硕士，主要从事免疫学及基因工程药物研究，E-mail: ncuskcaibaowei@163. com; 通讯作者及指导教师: 周克夫(1966 年－ ) ，男，博士，副教授，主要从事免疫学及基因工程药物研究，E-mail: [email protected]。厦门市科技项目(3502Z20083004); 国家“973”项目(2007CB513103); 福建省医药卫生创新项目(2009-CXB-51)资