目的:研究胸腺素α原(PrOTHyMOSInα,PrOTα)作为约氏疟原虫疫苗免疫佐剂的作用。方法:提取P.yOElII-17Xnl全蛋白作为抗原,用胸腺素α原作为免疫佐剂,免疫小鼠。具体方案为:昆明小鼠分为4组,每组6只,A组免疫P.yOElII-17Xnl全蛋白+PrOTα;b组免疫P.yOElII-17Xnl全蛋白;C组只注射PrOTα;d组为空白对照,以相同体积的生理盐水代替。免疫结束后感染致死的P.yOElII-17Xl,1x107个虫/只小鼠。结果:感染后的前10天A组小鼠疟原虫血症平均值要低于其他三组,且最终有3只小鼠存活下来,存活率50%,C组有一只小鼠存活,b、d组小鼠全部死亡。结论:用P.yOElII-17Xnl全蛋白做抗原,用PrOTα作为佐剂,比单独用P.yOElII-17Xnl全蛋白对小鼠有更好的免疫保护作用,提示了PrOTα可以成为一种有潜力的蛋白疫苗。Objective:To investigate the function of prothymosin α(ProTα) as vaccine adjuvant of P.yoelii.Methods:The mice were immunized with the total protein extracted from P.yoelii-17XNL as antigen,together with prothymosin α as adjuvant.Programs:Kunming mice were divided into A,B,C and D group.A group was immunized with P.yoelii-17XNL total protein and ProTα;B group was immunized with P.yoelii-17XNL;C group was only injected with ProTα;D group was the control,only injected with physiological saline.And then,the mice of each group was infected with P.yoelii-17XL,the dosage was 1×107/mice.Results:The parasitemia of A group-mice was lower than the other three groups in the first 10 days after infection,and eventually there were three mice survived in A group,the survival rate was 50%,one mouse survived in C group,all of mice in B group and D group died.Conclusion:Mice immunized with P.yoelii-17XNL total protein as antigen together with ProTα as adjuvants,had better immune protection than those immunized with P.yoelii-17XNL protein only.The present results suggest that the ProTα can act as a potential adjuvant in protein vaccine.厦门市科技项目(3502Z20083004);国家“973”项目(2007CB513103)资
