A QUICK METHOD TO MEASURE THE SAMPLE CUR-RENT OF THE FLOW CYTOMETER AND ITS ERROR MARGIN

第一作者: 彭兴跃, 出生于1966 年, 博士, 副教授。E-mail:xypeng @ xmu. edu. cn国家自然科学基金资助项目49806004号;高等学校骨干教师资助计划资助

应用光合色素标记物研究2001年2～6月厦门西海域浮游植物群落结构

利用反相高效液相色谱(RP-HPLC)对厦门西海域2001年2～6月的浮游植物色素进行了测定,并首次运用CHEMTAX软件对该海域进行了浮游植物群落组成的分析,同时分析了环境因子对群落组成的作用.我们首先改进了RP-HPLC分离方案,分离的时间缩短了近1/3,达到了提高分离效率的目的.利用改进后的RP-HPLC方案进行色素分析显示,厦门西海域2001年2～6月期间,主要的特征色素是岩藻黄素(fucoxanthin)、19’-丁酰基氧化岩藻黄素(19’-butanoyloxyfucoxanthin)、19’-己酰基氧化岩藻黄素(19’-hexanoyloxyfucoxanthin)和多甲藻素(peridinin),月平均含量分别在267.824～655.076、20.118～126.764、65.693～214.115、26.955～203.927μg/m3的范围变动.CHEMTAX计算的结果进一步表明:硅藻是主要优势类群,其次是定鞭金藻和甲藻,分别占总生物量的36.14%～79.27%、4.50%～37.40%和4.83%～21.37%.总生物量从2月到6月呈增长趋势,温度是主要的决定因子.硅藻和定鞭金藻所占生物量的份额呈相反趋势变化,表明种间竞争也是重要的因素之一

HPLC analysis of phytoplankton pigments in Western Xiamen Bay

通过2001年2月-11月对厦门港多个测点多时间尺度的定点观测，及6月、10月、11月三次走航调查，对厦门西海域的浮游植物光合色素时空分布进行研究，并以此探究浮游植物类群组成。主要结果如下： 1.研究所用的RP-HPLC-DAD色素分析技术是在王海黎的RP-HPLC-VIS方法基础上经改进后确定的，能分离鉴定15种色素，耗时短，并探讨了利用光合色素特征吸收光谱图的色素定性方法。 2.色素的时空分布： （1）厦门西海域范围内，特征类胡萝卜素是：岩藻黄素、多甲藻素、19′-己酰基氧化岩藻黄素，19′-丁酰基氧化岩藻黄素。岩藻黄素的浓度是该海域含量最高的类胡萝卜素；多甲藻素的分布不连续...This dissertation studies the temporal and spatial variabilities of phytoplankton photosynthetic pigments and then evaluated the temporal and spatial variabilities in the phytoplankton assemblages in Western Xiamen Bay. Improved RP-HPLC-DAD protocol was applied to analysis a large number of samples at several time series stations from Feb.2001 to Oct.2001 and three cruises that occurred in June, O...学位：理学硕士院系专业：生命科学学院生物学系_细胞生物学学号：20002602

The influence of lead on marine microalgae by a flow cytometry

运用流式细胞仪研究比较了Pb对3种常见海洋单细胞微藻:湛江叉鞭金藻(Dicrateriazhanjiangen sis)、球等鞭金藻(Isochrysisgalbana)、小球藻(Chlorellasp.)的毒害作用。用内插法求得Pb对3种微藻的96h的半有效浓度(96hEC50)分别为9.03、8.83和>20.00mg/L。Pb抑制细胞的生长分裂,使延滞期延长,并影响了叶绿素正常合成,毒性与浓度成正相关。Pb对不同生长期的细胞的作用强度不同,对生长分裂和叶绿素合成的作用程度呈现差异性。The effects of lead on the division and chlorophyll synthesis of Dicrateria zhanjiangensis, Isochrysis galbana and Chlorella sp. were studied using a flow cytometer. The results showed that the 96h·EC values of these three species of algae were 9.03 mg/L, 8.83 mg/L and >20.00 mg/L respectively. Lead inhibits the cell division, lengthened lag phase and caused abnormality of chlorophyll synthesis. Its toxity is increased as lead concentration increased. For any of the three species studied, the impacts of Lead on the cells of the two different phases of growth differed. It was also suggested that inhibition of chlorophyll synthesis was relatively insensitive to lead compared with that of the cell division rate.国家自然科学基金资助项目(49806004);; 高等学校骨干教师资助计划项

Photosynthetic pigments in seawater of Xiamen Harbor

应用反相高效液相色谱 (RP HPLC)方法分析测定了冬季厦门鼓浪屿水文观测站两周内不同潮位海水中的光合色素的组成与含量 ,包括多甲藻素、1 9 己酰基氧化岩藻黄素、硅甲藻黄素、硅藻黄素、玉米黄素、叶绿素a、脱镁叶绿素a、β 胡萝卜素等 .同时还实测了小球藻、金藻、盐藻、甲藻、角毛藻、螺旋藻等多个实验室培养藻种的色素组成 .数据表明 ,不同种类的藻类具有不同的色素组成特征 ,从海水中光合色素的分析数据可推测其浮游植物主要种类组成情况 .潮汐的水动力情况在色素组成变化上有所反映 ,表明港内外的浮游植物组成分布有梯度存在 ,且在特征上有所不同 .Photosynthetic pigments in seawater samples of Xiamen Harbor in winter have been measured by HPLC, and pigments of some cultured species of phytoplankton have also been measured, such as Chaetoceros sp., Prorocentrum cassubicum,Isochrysis galbana, Dunaliella salina, Spirulina platensis, Synechococcus sp. The data of about 15 kinds of pigments are obtained, including chloropyllide a, chlorophyll c, peridinin, 19'-butanoyloxyfucoxanthin, fucoxanthin, 19'-hexanoyloxyfucoxanthin,violaxanthin, diadinoxanthin, diatoxanthin, lutein, zeaxanthin, chlorophyll b, chlorophyll a, phaeophytin a, beta-carotene. The abilities of pigments indicating phytoplankton in seawater of Xiamen Harbor are discussed and results shows that tidal current is a most important controller to abundance and specific composition of phytoplankton and pigments indicate that diatom and chrysophyceae are the main phytoplankton.国家自然科学基金资助项目 (4 980 6 0 0 4

Study on Purification of Chinese Narcissus Viruses

研究建立了一个差速离心和蔗糖阶梯式密度离心的分离法 ,对感染水仙叶片的有关病毒进行分离、纯化 .发现有 3种不同的病毒颗粒 ,大小分别为 4 80～ 5 5 0× 12～ 13nm ;70 0～ 96 0× 12～13nm ;180～ 2 5 0× 12～ 13nm .SDS电泳发现 3种与病毒相关的蛋白质 .它们可能属于水仙黄条病毒、水仙花叶病毒和烟草脆裂病毒The present study establishes purification method of related viruses in infected leaves of narcissus by using disparity gradient centrifuge. The results show that there are three kinds of different particles of the viruses which are 480～550×12～13nm,800～960×12～13nm;and 180～250×12～13nm respectively.SDS eletropho esis analysis indicates that there are three kinds of proteins related to Narcissus viruses, which probably belong to narcissus yellow stripe virus, narcissus mosaic virus and tobravirus.福建省自然科学基金资助课题 (C970 0 1号 );; 漳州市科委资助项

JUNO Sensitivity on Proton Decay p→νˉK+p\to \bar\nu K^+ Searches

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this paper, the potential on searching for proton decay in $p\to \bar\nu K^+$ mode with JUNO is investigated.The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits to suppress the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar\nu K^+$ is 36.9% with a background level of 0.2 events after 10 years of data taking. The estimated sensitivity based on 200 kton-years exposure is $9.6 \times 10^{33}$ years, competitive with the current best limits on the proton lifetime in this channel

JUNO sensitivity on proton decay p → ν K + searches*

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this study, the potential of searching for proton decay in the $p\to \bar{\nu} K^+$ mode with JUNO is investigated. The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits suppression of the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar{\nu} K^+$ is 36.9% ± 4.9% with a background level of $0.2\pm 0.05({\rm syst})\pm$$0.2({\rm stat})$ events after 10 years of data collection. The estimated sensitivity based on 200 kton-years of exposure is $9.6 \times 10^{33}$ years, which is competitive with the current best limits on the proton lifetime in this channel and complements the use of different detection technologies