Diagnostiek van de exotische waterschimmelziekte EUS: resultaten van een club 5 project.

In de EU richtlijn 2006/88/EG staat op de lijst van exotische visziekten de ziekte EUS (Epizoötisch Ulceratief Syndroom). Tot nu toe waren slechts weinig laboratoria in Europa in staat deze ziekte aan te tonen. In dit artikel informatie over een project (EUS Club 5) dat daar verandering in heeft gebracht

The design thinking approaches of three different groups of designers based on self-reports

This paper compares the design thinking approaches of three groups of student-designers: industrial design and architecture undergraduates, and design PhD candidates. Participants responded to an open-ended design brief, working individually. Upon submission of their designs they were debriefed about their design processes. We compare the groups based on their submissions and self-reported design activities, especially the sequence of their design activities and the time allotted to them. There were some commonalities and differences between the two undergraduate groups but the main differences were between the two undergraduates and the PhD students. On the basis of the findings we pose questions regarding design methods in the era of 'design thinking' wherein designers are required to adopt an entrepreneurial frame of mind

Development and validation of a two-step real-time RT-PCR for the detection of eel virus European X in European eel, Anguilla anguilla

AbstractEel virus European X (EVEX) is one of the most common pathogenic viruses in farmed and wild European eel (Anguilla anguilla) in the Netherlands. The virus causes a hemorrhagic disease resulting in increased mortality rates. Cell culture and antibody-based detection of EVEX are laborious and time consuming. Therefore, a two-step real-time reverse transcriptase (RT-)PCR assay was developed for rapid detection of EVEX. Primers and probe for the assay were designed based on a sequence of the RNA polymerase or L gene of EVEX. The real-time RT-PCR assay was validated both for use with SYBR Green chemistry and for use with a TaqMan probe. The assay is sensitive, specific, repeatable, efficient and has a high r2-value. The real-time RT-PCR assay was further evaluated by testing field samples of European eels from the Netherlands, which were positive or negative for EVEX by virus isolation followed by an indirect fluorescent antibody test. The real-time RT-PCR assay allows rapid, sensitive and specific laboratory detection of EVEX in RNA extracts from 10% eel organ suspensions and cell cultures with cytopathic effects, and is a valuable contribution to the diagnosis of viral diseases of eel

On Abduction in Design

The mechanism of design reasoning from function to form is addressed by examining the possibility of explaining it as abduction. We propose a new interpretation to some definitions of innovative abduction, to show first that the concept, idea, as the basis for solution must be present in the inference, and second, that the reasoning from function to form is best modeled as a two-step inference, both of the innovative abduction pattern. This double-abductive reasoning is shown also to be the main form of reasoning in the empirically-derived “parameter analysis” method of conceptual design. Finally, the introduction of abduction into design theory is critically assessed, and in so doing, topics for future research are suggested

Eerste detectie van oesterherpesvirus OsHv-1 in Nederland

In het late voorjaar en zomer van 2008 en 2009 werd verhoogde sterfte geconstateerd onder Japanse oesters (Crassostrea gigas) in verscheidene kweekgebieden in Frankrijk, Ierland en de Kanaaleilanden. Het oesterherpesvirus, Ostreid herpesvirus 1 µvar (OsHV-1 µvar), lijkt een belangrijke rol te hebben gespeeld bij de sterfte. Gezien de mogelijkheid op herhaling van de problemen in het voorjaar en zomer van 2010 en de mogelijke verspreiding van OsHV-1 µvar zijn EU maatregelen opgesteld ter bestrijding van de verhoogde mortaliteit bij Japanse oesters in samenhang met de aanwezigheid van het OsHB-1 µvar. In Nederland is in 2010 een programma gestart voor de vroegtijdige detectie van OsHV-1 µvar. In dit artikel zijn de resultaten van de monitoring naar OsHV-1 in de zomer van 2010 in Nederland en de verspreiding van OsHV-1 in 2010 in Europa beschreve

Explicating concepts in reasoning from function to form by two-step innovative abductions

The mechanism of design reasoning from function to form is suggested to consist of a two-step inference of the innovative abduction type. First is an inference from a desired functional aspect to an idea, concept, or solution principle to satisfy the function. This is followed by a second innovative abduction, from the latest concept to form, structure, or mechanism. The intermediate entity in the logical reasoning, the concept, is thus made explicit, which is significant in following and understanding a specific design process, for educating designers, and to build a logic-based computational model of design. The idea of a two-step ab- ductive reasoning process is developed from the critical examination of several propositions made by others. We use the notion of innovative abduction in design, as opposed to such abduction where the question is about selecting among known alternatives, and we adopt a previously proposed two-step process of abductive reasoning. However, our model is different in that the two abductions used follow the syllogistic pattern of innovative abduction. In addition to using a schematic example from the literature to demonstrate our derivation, we apply the model to an existing, empirically derived method of conceptual design called “parameter analysis” and use two examples of real design processes. The two synthetic steps of the method are shown to follow the proposed double innovative abduction scheme, and the design processes are presented as sequences of double abductions from function to concept and from concept to form, with a subsequent deductive evaluation step

Public health and valorization of genome-based technologies: a new model

<p>Abstract</p> <p>Background</p> <p>The success rate of timely translation of genome-based technologies to commercially feasible products/services with applicability in health care systems is significantly low. We identified both industry and scientists neglect health policy aspects when commercializing their technology, more specifically, Public Health Assessment Tools (PHAT) and early on involvement of decision makers through which market authorization and reimbursements are dependent. While Technology Transfer (TT) aims to facilitate translation of ideas into products, Health Technology Assessment, one component of PHAT, for example, facilitates translation of products/processes into healthcare services and eventually comes up with recommendations for decision makers. We aim to propose a new model of valorization to optimize integration of genome-based technologies into the healthcare system.</p> <p>Methods</p> <p>The method used to develop our model is an adapted version of the Fish Trap Model and the Basic Design Cycle.</p> <p>Results</p> <p>We found although different, similarities exist between TT and PHAT. Realizing the potential of being mutually beneficial justified our proposal of their relative parallel initiation. We observed that the Public Health Genomics Wheel should be included in this relative parallel activity to ensure all societal/policy aspects are dealt with preemptively by both stakeholders. On further analysis, we found out this whole process is dependent on the Value of Information. As a result, we present our LAL (Learning Adapting Leveling) model which proposes, based on market demand; TT and PHAT by consultation/bi-lateral communication should advocate for relevant technologies. This can be achieved by public-private partnerships (PPPs). These widely defined PPPs create the innovation network which is a developing, consultative/collaborative-networking platform between TT and PHAT. This network has iterations and requires learning, assimilating and using knowledge developed and is called absorption capacity. We hypothesize that the higher absorption capacity, higher success possibility. Our model however does not address the phasing out of technology although we believe the same model can be used to simultaneously phase out a technology.</p> <p>Conclusions</p> <p>This model proposes to facilitate optimization/decrease the timeframe of integration in healthcare. It also helps industry and researchers to come to a strategic decision at an early stage, about technology being developed thus, saving on resources, hence minimizing failures.</p