PENGEMBANGAN PENGGUNAAN MULTIMEDIA INTERAKTIF BERBASIS FLASH UNTUK MENINGKATKAN PENGETAHUAN DAN KETERAMPILAN SISWA PADA MATA PELAJARAN KOMPUTER DAN JARINGAN DASAR POKOK BAHASAN PEMBUATAN KABEL JARINGAN KELAS X TEKNIK KOMPUTER JARINGAN

AbstrakMasih banyaknya siswa yang mengalami kesulitan pembelajaran dalam materi pembuatan kabel jaringan, mengingat pandemi Covid-19 yang memaksa pembelajaran di kelas harus dihentikan dan belum tersedianya media pembelajaran interaktif dalam proses pembelajaran. Oleh karena itu dibutuhkan media pembelajaran interaktif yang dapat membantu siswa dalam proses pembelajaran. Tujuan dari penelitian ini adalah mengembangkan multimedia interaktif dan menguji kelayakan dari media, mengetahui peningkatan pengetahuan dan keterampilan siswa setelah menggunakan multimedia interaktif. Metode yang digunakan adalah metode penelitian pengembangan (R&D). Model pengembangan yang digunakan pada penelitian ini adalah model pengembangan Luther yang terdiri dari: concept, design, material collecting, assembly, testing dan distribution. Subjek penelitiannya adalah 36 siswa. Pengujian dilakukan dengan dua tahap yaitu pengujian alpha dan beta. Setelah itu, siswa mengerjakan tes tulis dan praktik untuk mengetahui peningkatan pengetahuan dan keterampilannya. Metode analisis data menggunakan metode kuantitatif dengan desain penelitian one group pretest posttest design. Dalam penelitian ini analisis regresi linier sederhana digunakan untuk menentukan pengaruh antara variabel. Hasil penelitian menunjukkan bahwa multimedia interaktif sangat layak digunakan sebagai alat bantu pembelajaran. Hasil penelitian juga menunjukkan bahwa ada hubungan yang positif dan signifikan antara penggunaan multimedia interaktif dengan pengetahuan siswa. Hubungan yang positif dan signifikan juga terjadi pada penggunaan multimedia interaktif dengan keterampilan siswa. Implikasi dari hasil penelitian ini adalah adanya pengaruh yang signifikan antara penggunaan multimedia interaktif terhadap pengetahuan dan keterampilan siswa.Kata Kunci: Multimedia Interaktif, Pengetahuan, KeterampilanAbstractThere are still many students who have difficulty learning in the course of making network cables, given the Covid-19 pandemic which forces classroom learning to be stopped and there are no interactive learning media available in the learning process. Therefore, interactive learning media are needed that can help students in the learning process. The purpose of this research is to develop interactive multimedia and test the feasibility of the media, to determine the increase in knowledge and skills of students after using interactive multimedia. The method used is the method of research and development (R&D). The development model used in this research is Luther's development model which consists of concept, design, material collecting, assembly, testing, and distribution. The research subjects were 36 students. The test is carried out in two stages, namely alpha and beta testing. After that, students take written and practical tests to determine the increase in knowledge and skills. Methods of data analysis using quantitative methods with a one-group pretest-posttest design research design. In this study, simple linear regression analysis was used to determine the influence between variables. The results showed that interactive multimedia is very suitable to be used as a learning aid. The results also show that there is a positive and significant relationship between the use of interactive multimedia and students' knowledge. A positive and significant relationship also occurs in the use of interactive multimedia with students' skills. The results of this study imply that there is a significant influence between the use of interactive multimedia on students' knowledge and skills.Keyword: Interactive Multimedia, Knowledge, Skills

CORPO E ESCOLA:: O “CUIDADO DE SI” COMO “ATITUDE MODERNA”

Apresentamos os resultados dos nossos estudos entre os processos educacionais e processos de subjetivação. Associamos este fato com a dificuldade dos educadores deslocarem o corpo do lugar de quem ensina para o lugar de quem aprende. Estudamos as implicações do corpo nos processos educacionais e sugerimos uma “atitude moderna” a partir do “cuidado de si”, apoiados em Foucault, como a ação do educador que “toma partido” do educando

Follistatin-like 3 (FSTL3) mediated silencing of transforming growth factor (TGF ) signaling is essential for testicular aging and regulating testis size

Follistatin-like 3 (FSTL3) is a glycoprotein that binds and inhibits the action of TGFβ ligands such as activin. The roles played by FSTL3 and activin signaling in organ development and homeostasis are not fully understood. The authors show mice deficient in FSTL3 develop markedly enlarged testes that are also delayed in their age-related regression. These FSTL3 knockout mice exhibit increased Sertoli cell numbers, allowing for increased spermatogenesis but otherwise showing normal testicular function. The data show that FSTL3 deletion leads to increased AKT signaling and SIRT1 expression in the testis. This demonstrates a cross-talk between TGFβ ligand and AKT signaling and leads to a potential mechanism for increased cellular survival and antiaging. The findings identify crucial roles for FSTL3 in limiting testis organ size and promoting age-related testicular regression

BMP Signaling in the Human Fetal Ovary is Developmentally Regulated and Promotes Primordial Germ Cell Apoptosis

Primordial germ cells (PGCs) are the embryonic precursors of gametes in the adult organism, and their development, differentiation, and survival are regulated by a combination of growth factors collectively known as the germ cell niche. Although many candidate niche components have been identified through studies on mouse PGCs, the growth factor composition of the human PGC niche has not been studied extensively. Here we report a detailed analysis of the expression of components of the bone morphogenetic protein (BMP) signaling apparatus in the human fetal ovary, from postmigratory PGC proliferation to the onset of primordial follicle formation. We find developmentally regulated and reciprocal patterns of expression of BMP2 and BMP4 and identify germ cells to be the exclusive targets of ovarian BMP signaling. By establishing long-term cultures of human fetal ovaries in which PGCs are retained within their physiological niche, we find that BMP4 negatively regulates postmigratory PGC numbers in the human fetal ovary by promoting PGC apoptosis. Finally, we report expression of both muscle segment homeobox (MSX)1 and MSX2 in the human fetal ovary and reveal a selective upregulation of MSX2 expression in human fetal ovary in response to BMP4, suggesting this gene may act as a downstream effector of BMP-induced apoptosis in the ovary, as in other systems. These data reveal for the first time growth factor regulation of human PGC development in a physiologically relevant context and have significant implications for the development of cultures systems for the in vitro maturation of germ cells, and their derivation from pluripotent stem cells

3640 Unique EST Clusters from the Medaka Testis and Their Potential Use for Identifying Conserved Testicular Gene Expression in Fish and Mammals

BACKGROUND: The fish medaka is the first vertebrate capable of full spermatogenesis in vitro from self-renewing spermatogonial stem cells to motile test-tube sperm. Precise staging and molecular dissection of this process has been hampered by the lack of suitable molecular markers. METHODOLOGY AND PRINCIPAL FINDINGS: We have generated a normalized medaka testis cDNA library and obtained 7040 high quality sequences representing 3641 unique gene clusters. Among these, 1197 unique clusters are homologous to known genes, and 2444 appear to be novel genes. Ontology analysis shows that the 1197 gene products are implicated in diverse molecular and cellular processes. These genes include markers for all major types of testicular somatic and germ cells. Furthermore, markers were identified for major spermatogenic stages ranging from spermatogonial stem cell self-renewal to meiosis entry, progression and completion. Intriguingly, the medaka testis expresses at least 13 homologs of the 33 mouse X-chromosomal genes that are enriched in the testis. More importantly, we show that key components of several signaling pathways known to be important for testicular function in mammals are well represented in the medaka testicular EST collection. CONCLUSIONS/SIGNIFICANCE: Medaka exhibits a considerable similarity in testicular gene expression to mammals. The medaka testicular EST collection we obtained has wide range coverage and will not only consolidate our knowledge on the comparative analysis of known genes' functions in the testis but also provide a rich resource to dissect molecular events and mechanism of spermatogenesis in vivo and in vitro in medaka as an excellent vertebrate model

Mechanically Induced Chromatin Condensation Requires Cellular Contractility in Mesenchymal Stem Cells

This work was supported by the National Institutes of Health (R01 AR056624, R01 EB02425, T32 AR007132, and P30 AR050950). Additional support was provided by a Montague Research Award from the Perelman School of Medicine and a University of Pennsylvania University Research Foundation Award