Greenhouse gas reporting for the LULUCF sector in the Netherlands : Methodological background, update 2022

This report provides a complete methodological description and background information of the Dutch National System for Greenhouse gas reporting of the LULUCF sector. It provides detailed description of the methodologies, activity data and emission factors that were used. Each of the reporting categories Forest Land, Cropland, Grassland, Wetlands, Settlements, Other land and Harvested Wood Products are described in a separate chapter. Additionally, it gives a table-by-table elaboration of the choices and motivations for filling the CRF tables for KP-LULUCF

Mutations in the DJ-1 gene associated with autosomal recessive early-onset parkinsonism.

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Ex vivo detectable activation of Melan-A-specific T cells correlating with inflammatory skin reactions in melanoma patients vaccinated with peptides in IFA.

The purpose of this study was to test melanoma vaccines consisting of peptides and immunological adjuvants for optimal immunogenicity and to evaluate laboratory immune monitoring for in vivo relevance. Forty-nine HLA-A2 positive patients with Melan-A positive melanoma were repeatedly vaccinated with Melan-A peptide, with or without immune adjuvant AS02B (QS21 and MPL) or IFA. Peptide-specific CD8 T cells in PBLs were analyzed ex vivo using fluorescent HLA-A2/Melan-A multimers and IFN-gamma ELISPOT assays. The vaccines were well tolerated. In vivo expansion of Melan-A-specific CD8 T cells was observed in 13 patients (1/12 after vaccination with peptide in AS02B and 12/17 after vaccination with peptide in IFA). The T cells produced IFN-gamma and downregulated CD45RA and CD28. T-cell responses correlated with inflammatory skin reactions at vaccine injection sites (P < 0.001) and with DTH reaction to Melan-A peptide (P < 0.01). Twenty-six of 32 evaluable patients showed progressive disease, whereas 4 patients had stable disease. The two patients with the strongest Melan-A-specific T-cell responses experienced regression of metastases in skin, lymph nodes, and lung. We conclude that repeated vaccination with Melan-A peptide in IFA frequently leads to sustained responses of specific CD8 T cells that are detectable ex vivo and correlate with inflammatory skin reactions

Outcome of isolated gastroschisis; an international study, systematic review and meta-analysis

Research into fetal development and medicin