627 research outputs found
In-depth quantitative proteomics uncovers specie-specific metabolic programs in Leishmania (Viannia) species
Author summary Leishmania braziliensis,L.panamensis, andL.guyanensisare responsible for most of the cases of tegumentary leishmaniasis (TL) in the Americas. These species are associated with a variety of clinical manifestations of TL ranging from self-healing localized cutaneous lesions to disseminated and mucocutaneous presentations that may result in severe oropharyngeal mutilation. Here, we report a comprehensive quantitative comparison of the proteome of those species. Assessment of absolute titers of similar to 7000 proteins revealed a very clear differentiation among them. Significant differences in energy metabolism, membrane proteins, transporters, and lipids are contributing for species-specific traits and provide rich substrate for exploring new molecules for diagnosing purposes. Leishmaniaspecies are responsible for a broad spectrum of diseases, denominated Leishmaniasis, affecting over 12 million people worldwide. During the last decade, there have been impressive efforts for sequencing the genome of most of the pathogenicLeishmaniaspp. as well as hundreds of strains, but large-scale proteomics analyses did not follow these achievements and theLeishmaniaproteome remained mostly uncharacterized. Here, we report a comprehensive comparative study of the proteomes of strains representingL.braziliensis,L.panamensisandL.guyanensisspecies. Proteins extracted by SDS-mediated lysis were processed following the multi-enzyme digestion-filter aided sample preparation (FASP) procedure and analysed by high accuracy mass spectrometry. "Total Protein Approach" and "Proteomic Ruler" were applied for absolute quantification of proteins. Principal component analysis demonstrated very high reproducibility among biological replicates and a very clear differentiation of the three species. Our dataset comprises near 7000 proteins, representing the most completeLeishmaniaproteome yet known, and provides a comprehensive quantitative picture of the proteomes of the three species in terms of protein concentration and copy numbers. Analysis of the abundance of proteins from the major energy metabolic processes allow us to highlight remarkably differences among the species and suggest that these parasites depend on distinct energy substrates to obtain ATP. WhereasL.braziliensisrelies the more on glycolysis,L.panamensisandL.guyanensisseem to depend mainly on mitochondrial respiration. These results were confirmed by biochemical assays showing opposite profiles for glucose uptake and O(2)consumption in these species. In addition, we provide quantitative data about different membrane proteins, transporters, and lipids, all of which contribute for significant species-specific differences and provide rich substrate for explore new molecules for diagnosing purposes. Data are available via ProteomeXchange with identifier PXD017696
Produção de biomassa de banana verde.
bitstream/item/211941/1/ComunicadoTecnico171-Eliseth-AINFO.pd
Measurement of Semileptonic Branching Fractions of B Mesons to Narrow D** States
Using the data accumulated in 2002-2004 with the DO detector in
proton-antiproton collisions at the Fermilab Tevatron collider with
centre-of-mass energy 1.96 TeV, the branching fractions of the decays B ->
\bar{D}_1^0(2420) \mu^+ \nu_\mu X and B -> \bar{D}_2^{*0}(2460) \mu^+ \nu_\mu X
and their ratio have been measured: BR(\bar{b}->B) \cdot BR(B-> \bar{D}_1^0
\mu^+ \nu_\mu X) \cdot BR(\bar{D}_1^0 -> D*- pi+) =
(0.087+-0.007(stat)+-0.014(syst))%; BR(\bar{b}->B)\cdot BR(B->D_2^{*0} \mu^+
\nu_\mu X) \cdot BR(\bar{D}_2^{*0} -> D*- \pi^+) =
(0.035+-0.007(stat)+-0.008(syst))%; and (BR(B -> \bar{D}_2^{*0} \mu^+ \nu_\mu
X)BR(D2*0->D*- pi+)) / (BR(B -> \bar{D}_1^{0} \mu^+ \nu_\mu X)\cdot
BR(\bar{D}_1^{0}->D*- \pi^+)) = 0.39+-0.09(stat)+-0.12(syst), where the charge
conjugated states are always implied.Comment: submitted to Phys. Rev. Let
Measurement of the Lifetime Difference in the B_s^0 System
We present a study of the decay B_s^0 -> J/psi phi We obtain the CP-odd
fraction in the final state at time zero, R_perp = 0.16 +/- 0.10 (stat) +/-
0.02 (syst), the average lifetime of the (B_s, B_sbar) system, tau (B_s^0)
=1.39^{+0.13}_{-0.16} (stat) ^{+0.01}_{-0.02} (syst) ps, and the relative width
difference between the heavy and light mass eigenstates, Delta Gamma/Gamma =
(Gamma_L - Gamma_H)/Gamma =0.24^{+0.28}_{-0.38} (stat) ^{+0.03}_{-0.04} (syst).
With the additional constraint from the world average of the B_s^0$lifetime
measurements using semileptonic decays, we find tau (B_s^0)= 1.39 +/- 0.06 ~ps
and Delta Gamma/\Gamma = 0.25^{+0.14}_{-0.15}. For the ratio of the B_s^0 and
B^0 lifetimes we obtain tau(B_s^0)/tau(B^0)} = 0.91 +/- 0.09 (stat) +/- 0.003
(syst).Comment: submitted to Phys. Rev. Lett. FERMILAB-PUB-05-324-
Search for Large Extra Spatial Dimensions in Dimuon Production with the D0 Detector
We present the results of a search for the effects of large extra spatial
dimensions in collisions at 1.96 TeV in events
containing a pair of energetic muons. The data correspond to 246 \ipb of
integrated luminosity collected by the \D0 experiment at the Fermilab Tevatron
Collider. Good agreement with the expected background was found, yielding no
evidence for large extra dimensions. We set 95% C.L. lower limits on the
fundamental Planck scale between 0.85 TeV and 1.27 TeV within several
formalisms. These are the most stringent limits achieved in the dimuon channel
to date.Comment: 8 pages, 3 figures, 1 table. Published in Phys. Rev. Lett. Minor
changes in v2 to match the published versio
Search for R-parity violating supersymmetry via the LLE couplings lambda_{121}, lambda_{122} or lambda_{133} in ppbar collisions at sqrt(s)=1.96 TeV
A search for gaugino pair production with a trilepton signature in the
framework of R-parity violating supersymmetry via the couplings lambda_121,
lambda_122, or lambda_133 is presented. The data, corresponding to an
integrated luminosity of L~360/pb, were collected from April 2002 to August
2004 with the D0 detector at the Fermilab Tevatron Collider, at a
center-of-mass energy of sqrt(s)=1.96 TeV. This analysis considers final states
with three charged leptons with the flavor combinations eel, mumul, and eetau
(l=e or mu). No evidence for supersymmetry is found and limits at the 95%
confidence level are set on the gaugino pair production cross section and lower
bounds on the masses of the lightest neutralino and chargino are derived in two
supersymmetric models.Comment: 9 pages, 4 figures (fig2 includes 3 subfigures
Search for right-handed W bosons in top quark decay
We present a measurement of the fraction f+ of right-handed W bosons produced
in top quark decays, based on a candidate sample of events in the
lepton+jets decay mode. These data correspond to an integrated luminosity of
230pb^-1, collected by the DO detector at the Fermilab Tevatron
Collider at sqrt(s)=1.96 TeV. We use a constrained fit to reconstruct the
kinematics of the and decay products, which allows for the
measurement of the leptonic decay angle for each event. By comparing
the distribution from the data with those for the expected
background and signal for various values of f+, we find
f+=0.00+-0.13(stat)+-0.07(syst). This measurement is consistent with the
standard model prediction of f+=3.6x10^-4.Comment: Submitted to Physical Review D Rapid Communications 7 pages, 3
figure
Measurement of the Lifetime Using Semileptonic Decays
We report a measurement of the lifetime in the semileptonic decay
channel (and its charge conjugate), using
approximately 0.4 fb of data collected with the D0 detector during 2002
-- 2004. We have reconstructed 5176 signal events, where the
is identified via the decay , followed by . Using these events, we have measured the lifetime to be
. This is the most precise measurement of the lifetime to date.Comment: To appear in Phys. Rev. Lett., 7 pages, 2 figure
Measurement of the ppbar to ttbar production cross section at sqrt(s)=1.96 TeV in the fully hadronic decay channel
A measurement of the top quark pair production cross section in proton
anti-proton collisions at an interaction energy of sqrt(s)=1.96 TeV is
presented. This analysis uses 405 pb-1 of data collected with the D0 detector
at the Fermilab Tevatron Collider. Fully hadronic ttbar decays with final
states of six or more jets are separated from the multijet background using
secondary vertex tagging and a neural network. The ttbar cross section is
measured as sigma(ttbar)=4.5 -1.9 +2.0 (stat) -1.1 +1.4 (syst) +/- 0.3 (lumi)
pb for a top quark mass of m(t) = 175 GeV/c^2.Comment: 10 pages, 10 figures, submitted to Phys. Rev.
Measurement of the B0_s semileptonic branching ratio to an orbitally excited D_s** state, Br(B0_s -> Ds1(2536) mu nu)
In a data sample of approximately 1.3 fb-1 collected with the D0 detector
between 2002 and 2006, the orbitally excited charm state D_s1(2536) has been
observed with a measured mass of 2535.7 +/- 0.6 (stat) +/- 0.5 (syst) MeV via
the decay mode B0_s -> D_s1(2536) mu nu X. A first measurement is made of the
branching ratio product Br(b(bar) -> D_s1(2536) mu nu X).Br(D_s1(2536)->D*
K0_S). Assuming that D_s1(2536) production in semileptonic decay is entirely
from B0_s, an extraction of the semileptonic branching ratio Br(B0_s ->
D_s1(2536) mu nu X) is made.Comment: 7 pages, 2 figures, LaTeX, version with minor changes as accepted by
Phys. Rev. Let
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