Tocopherol and fatty acids content and proximal composition of four avocado cultivars (Persea Americana Mill)

Avocado pulp is widely regarded as a great source of edible oil containing fat-soluble vitamins and omega-3 fatty acids (FA). However, avocado peel and seeds are also good sources of edible oil and are less explored byproducts. This paper aimed at determining the proximal composition, FA and tocopherol contents of the pulp, peel, and seeds of Quintal, Fortuna, Margarida, and Hass avocado cultivars. The pulps presented high concentrations of oleic acid. In addition, peel and seeds presented lower omega-6/omega-3 ratios than the pulp. There was also a considerable amount of tocopherol in the peel and seeds, especially in Hass peel (230.7 mg/100 g). According to the results, the peel and seeds of avocado that are considered byproducts, can be utilized in food industry

Reliability and validity of body weight and body image perception in children and adolescents from the South American Youth/Child Cardiovascular and Environmental (SAYCARE) Study

Objective: To assess the reliability and validity of body weight (BW) and body image (BI) perception reported by parents (in children) and by adolescents in a South American population. Design: Cross-sectional study. BW perception was evaluated by the question, "Do you think you/your child are/is: severely wasted, wasted, normal weight, overweight, obese?" BI perception was evaluated using the Gardner scale. To evaluate reliability, BW and BI perceptions were reported twice, two weeks apart. To evaluate validity, the BW and BI perceptions were compared with WHO BMI Z-scores. Kappa and Kendall's tau-c coefficients were obtained. Setting: Public and private schools and high schools from six countries of South America (Argentina, Peru, Colombia, Uruguay, Chile, Brazil). Participants: Children aged 3-10 years (n 635) and adolescents aged 11-17 years (n 400). Results: Reliability of BW perception was fair in children's parents (k=0·337) and substantial in adolescents (k=0·709). Validity of BW perception was slight in children's parents (k=0·176) and fair in adolescents (k=0·268). When evaluating BI, most children were perceived by parents as having lower weight. Reliability of BI perception was slight in children's parents (k=0·124) and moderate in adolescents (k=0·599). Validity of BI perception was poor in children's parents (k=-0·018) and slight in adolescents (k=0·023). Conclusions: Reliability of BW and BI perceptions was higher in adolescents than in children's parents. Validity of BW perception was good among the parents of the children and adolescents with underweight and normal weight

High-resolution monitoring of diffuse (sheet or interrill) erosion using structure-from-motion

Sheet erosion is common on agricultural lands, and understanding the dynamics of the erosive process as well as the quantification of soil loss is important for both soil scientists and managers. However, measuring rates of soil loss from sheet erosion has proved difficult due to requiring the detection of relatively small surface changes over extended areas. Consequently, such measurements have relied on the use of erosion plots, which have limited spatial coverage and have high operating costs. For measuring the larger erosion rates characteristic of rill and gully erosion, structure-from-motion (SfM) photogrammetry has been demonstrated to be a valuable tool. Here, we demonstrate the first direct validation of UAV-SfM measurements of sheet erosion using sediment collection data collected from erosion plots. Three erosion plots (12 m × 4 m) located at Lavras, Brazil, with bare soil exposed to natural rainfall from which event sediment and runoff was monitored, were mapped during two hydrological years (2016 and 2017), using a UAV equipped with a RGB camera. DEMs of difference (DoD) were calculated to detect spatial changes in the soil surface topography over time and to quantify the volumes of sediments lost or gained. Precision maps were generated to enable precision estimates for both DEMs to be propagated into the DoD as spatially variable vertical uncertainties. The point clouds generated from SfM gave mean errors of ~2.4 mm horizontally (xy) and ~1.9 mm vertically (z) on control and independent check points, and the level of detection (LoD) along the plots ranged from 1.4 mm to 7.4 mm. The soil loss values obtained by SfM were significantly (p < 0.001) correlated (r 2 = 95.55%) with those derived from the sediment collection. These results open up the possibility to use SfM for erosion studies where channelized erosion is not the principal mechanism, offering a cost-effective method for gaining new insights into sheet, and interrill, erosion processes

Histone variant MacroH2A1 is downregulated in prostate cancer and influences malignant cell phenotype

Background: Prostate cancer (PCa), a major cause of cancer-related morbidity and mortality worldwide and mostly asymptomatic at earliest stages, is characterized by disruption of genetic and epigenetic balance. A better understanding of how those mechanisms orchestrate disease might improve diagnostic and prognostic tools, allowing for improvements in treatment efficacy. Replacement of canonical histones, an epigenetic mechanism, is highly conserved among species and altered expression of histones variants (e.g., MacroH2A1) has been associated with tumorigenesis. H2AFY gene encodes two isoforms of H2A histone variant MacroH2A1: MacroH2A1.1 and MacroH2A1.2. Specifically, MacroH2A1.1 isoform inhibits cell proliferation and promotes cellular differentiation. Because the contribution of this histone variant to carcinogenesis has been reported in several cancer types, but not for PCa, we aimed to investigate the contribution of MacroH2A1 for prostate carcinogenesis. Methods: MacroH2A1, MacroH2A1.1 and MacroH2A1.2 isoforms and the corresponding splicing regulators transcript levels were evaluated by RT-qPCR, in a tissue cohort composed by PCa, prostatic intraepithelial neoplasia (PIN) and normal prostate cases. Knockdown for MacroH2A1 and MacroH2A1.1 was performed through lentiviral transduction in DU145 cells, and MacroH2A1.1 overexpression was achieved in LNCaP cells by plasmid transfection, followed by functional assays. Biological and/or experimental replicates were performed when necessary, and specific statistical tests were applied to perform data analysis. Results: MacroH2A1.1 transcript levels were downregulated in PIN and primary PCa compared to normal prostate tissues. The same was found for QKI, a MacroH2A1.1's splicing regulator. Moreover, lower MacroH2A1.1 and QKI expression levels associated with less differentiated tumors (Gleason score ≥ 7). Interestingly, MacroH2A1.1, but more impressively DDX17 (AUC = 0.93; p < 0.0001) and QKI (AUC = 0.94; p < 0.0001), accurately discriminated cancerous from noncancerous prostate tissues. Furthermore, in PCa cell lines, total MacroH2A1 knockdown augmented malignant features, whereas MacroH2A1.1 overexpression impressively attenuated the malignant phenotype. Conclusions: Overall, our data, derived from primary PCa tissues and cell lines, anticipate a tumor suppressive role for MacroH2A1, particularly for the MacroH2A1.1 isoform, in prostate carcinogenesis

Economic analysis of an agrosilvipastoral system for a mountainous area in Zona da Mata Mineira, Brazil

The objective of this work was to evaluate the economic viability of an agrosilvipastoral system developed for Zona da Mata mountainous areas in Minas Gerais state, Brazil, as well as to compare different options for wood (Eucalyptus grandis and Acacia mangium) commercialization of the second thinning. The data were obtained from a 10 year-old agrosilvipastoral system established in four hectares at Embrapa Gado de Leite station in Coronel Pacheco, MG, Brazil. As evaluation criteria for the economic viability analysis, the adopted methods were the net present value (NPV) and the internal rate of return (IRR), both calculated at 6% interest rate. Despite the small difference, adding value to forest products increased the attractiveness of the proposed system. Considered separately, the agricultural activity was impracticable, whereas the forestry and livestock activities were independently viable. The studied system seems to be equally tolerant to price variations for forest and livestock products, as well as strongly tolerant to variations in production costs

Critical points on growth curves in autoregressive and mixed models

Adjusting autoregressive and mixed models to growth data fits discontinuous functions, which makes it difficult to determine critical points. In this study we propose a new approach to determine the critical stability point of cattle growth using a first-order autoregressive model and a mixed model with random asymptote, using the deterministic portion of the models. Three functions were compared: logistic, Gompertz, and Richards. The Richards autoregressive model yielded the best fit, but the critical growth values were adjusted very early, and for this purpose the Gompertz model was more appropriate