22 research outputs found
Fungi in a One Health Perspective
(This article belongs to the Collection Encyclopedia of Fungi)ReviewFungi constitute a diverse group with highly positive and negative impacts in different
environments, having several natural roles and beneficial applications in human life, but also causing
several concerns. Fungi can affect human health directly, but also indirectly by being detrimental
for animal and plant health, influencing food safety and security. Climate changes are also affecting
fungal distribution, prevalence, and their impact on different settings. Searching for sustainable
solutions to deal with these issues is challenging due to the complex interactions among fungi and
agricultural and forestry plants, animal production, environment, and human and animal health. In
this way, the “One Health” approach may be useful to obtain some answers since it recognizes that
human health is closely connected to animal and plant health, as well as to the shared environment.
This review aims to explore and correlate each of those factors influencing human health in this “One
Health” perspective. Thus, the impact of fungi on plants, human, and animal health, and the role of
the environment as an influencing factor on these elements are discussed.FCT—Fundação para a Ciência e Tecnologia, I.P., supported D.S. via a Ph.D. fellowship, UI/BD/154444/2022.info:eu-repo/semantics/publishedVersio
NEMÁTODES DE QUISTO DA BATATEIRA – O USO DE VARIEDADES RESISTENTES COMO ESTRATÉGIA DE CONTROLO
Potato cyst nematodes (PCN), Globodera rostochiensis and G. pallida, are considered harmful quarantine organisms and can be devastating to potato fields if not controlled in a timely manner. Its designation derives from the fact that the female body, after their death, turns into a spherical cyst con- taining the eggs, which are protected from adverse conditions even in the absence of hosts. PCN are present in all potato pro- ducing regions of the country, including the Madeira and Azores islands. PCN control involves their opportune detection, cor- rect identification and appropriate control measures. Tests already carried out proved that the use of resistant varieties is effective for the management of these organisms. However, there is insufficient choice of par- tially resistant cultivars for growers, espe- cially for the control of G. pallida
FTA-LAMP based biosensor for a rapid in-field detection of Globodera pallida—the pale potato cyst nematode
The combination of a sensitive and specific magnetoresistive sensing device with an easy DNA extraction method and a rapid isothermal amplification is presented here targeting the on-site detection of Globodera pallida, a potato endoparasitic nematode. FTA-cards were used for DNA extraction, LAMP was the method developed for DNA amplification and a nanoparticle functionalized magnetic-biosensor was used for the detection. The combinatorial effect of these three emerging technologies has the capacity to detect G. pallida with a detection limit of one juvenile, even when mixed with other related species. This combined system is far more interesting than what a single technology can provide. Magnetic biosensors can be combined with any DNA extraction protocol and LAMP forming a new solution to target G. pallida. The probe designed in this study consistently distinguished G. pallida (∆Vacbinding/Vacsensor above 1%) from other cyst nematodes (∆Vacbinding/Vacsensor below 1%). It was confirmed that DNA either extracted with FTA-cards or Lab extraction Kit was of enough quantity and quality to detect G. pallida whenever present (alone or in mixed samples), ensuring probe specificity and sensitivity. This work provides insights for a new strategy to construct advanced devices for pathogens in-field diagnostics. LAMP runs separately but can be easily integrated into a single device
Comparison of the performance of the main real-time and conventional PCR detection tests for ‘Candidatus Liberibacter’ spp., plant pathogenic bacteria causing the Huanglongbing disease in Citrus spp
23 Pág.Huanglongbing disease affects the Rutaceae family and is associated with three phloem-limited bacterium species: Candidatus Liberibacter asiaticus, africanus and americanus. These species are considered quarantine pathogens in the world, and pose major risks for citrus production and industry. Due to the low titer and the uneven distribution of the bacteria within its host plant, conventional PCR detection protocols can lead to false negative results, especially for early detection. Herein, three real-time PCR diagnostic methods recommended by the EPPO and FAO for asiaticus and africanus species detection were evaluated for their performance and compared with a conventional duplex PCR. Assessments were done as part of an international cooperative project under the EUPHRESCO guidance. Intra-laboratory assessment of the analytical specificity and analytical sensitivity was performed on 33 target or non-target DNA samples and seven target DNA samples were used to determine the sensitivity. Thereafter, repeatability, reproducibility, and concordance odds ratio were assessed on 20 target or non-target DNA samples through a collaborative test performance study organized among eight international laboratories. Results showed that the Li protocol proved to be the best method for asiaticus and africanus species detection, along with the conventional duplex PCR; whereas the Morgan protocol showed high performance only for asiaticus species. Interlaboratory reproducibility was high, suggesting that these real-time PCR methods can be readily transferred to diagnostic laboratories.This research was funded by Anses - Plant Health Laboratory (LSV).Peer reviewe
Fungi in a One Health Perspective
Fungi constitute a diverse group with highly positive and negative impacts in different environments, having several natural roles and beneficial applications in human life, but also causing several concerns. Fungi can affect human health directly, but also indirectly by being detrimental for animal and plant health, influencing food safety and security. Climate changes are also affecting fungal distribution, prevalence, and their impact on different settings. Searching for sustainable solutions to deal with these issues is challenging due to the complex interactions among fungi and agricultural and forestry plants, animal production, environment, and human and animal health. In this way, the “One Health” approach may be useful to obtain some answers since it recognizes that human health is closely connected to animal and plant health, as well as to the shared environment. This review aims to explore and correlate each of those factors influencing human health in this “One Health” perspective. Thus, the impact of fungi on plants, human, and animal health, and the role of the environment as an influencing factor on these elements are discussed
Development and Validation of a Loop-Mediated Isothermal Amplification Diagnostic Method to Detect the Quarantine Potato Pale Cyst Nematode, Globodera pallida
The potato cyst nematode (PCN) Globodera pallida has acquired significant importance throughout Europe due to its nefarious effects on potato production. Rapid and reliable diagnosis of PCN is critical during the surveillance programs and for the implementation of control measures. Molecular DNA-based methods are available, but they require expensive laboratory facilities, equipment and trained technicians. Moreover, there is an additional need of time for sample shipment and testing. In this work, we have developed a new and simple assay which reliably discriminates G. pallida from other cyst nematodes in less than 40 min. This assay may be applied either on cysts or juveniles with the ability to detect a single juvenile of G. pallida in a sample of at least 40 juveniles of the non-target species G. rostochiensis. This test should be a tool to improve the performance of the laboratory and has the potential to be performed on-site
Integrative Morphometric and Molecular Approach to Update the Impact and Distribution of Potato Cyst Nematodes Globodera rostochiensis and Globodera pallida (Tylenchida: Heteroderidae) in Algeria
Morphological and molecular studies were conducted to characterize the specific identity of 36 isolates of potato cyst nematodes (PCNs) recovered from soil samples collected in several potato producing areas of Algeria. Morphometric data revealed that 44% of isolates contained Globodera pallida alone, 28% contained Globodera rostochiensis alone and 28% mixtures of the two species. Morphometric values of cysts and second-stage juveniles were generally distributed with slight differences in the expected ranges for both Globodera species. Inter- and intraspecific morphometric variability in nematode isolates was noted. Molecular analysis using conventional multiplex PCR with species-specific primers and TaqMan real-time PCR confirmed the morphological identification. In addition, the distribution of both potato cyst nematode species throughout various parts of the country was investigated. In the central areas, the isolates of G. pallida alone dominate, whereas isolates of G. rostochiensis alone are more frequent in the southern areas. In the eastern regions, mixed isolates are more representative. Most isolates examined in the western areas are mixtures of the two species or G. rostochiensis alone. Comparatively, G. pallida remains the most widely distributed species in its geographic range. This study confirms the presence of two PCN species, G. pallida and G. rostochiensis, in Algeria and provides additional information on their biogeographic distribution
Exploratory Study in Maize Farms
Funding Information: This research was funded by National Funds by Rural Development Program through the Operational Group QUALIMILHO—New sustainable integration strategies that guarantee quality and safety in the national maize, PDR2020 n° 101-031295 (2017–2020). This work was also supported by FCT, Portuguese Foundation for Science and Technology through the R&D Unit, UIDB/04551/2020 (GREEN-IT, Bioresources for Sustainability), the projects UIDB/00211/2020 and UIDB/04033/2020. Publisher Copyright: © 2023 by the authors.Maize is a significant crop to the global economy and a key component of food and feed, although grains and whole plants can often be contaminated with mycotoxins resulting in a general exposure of the population and animals. To investigate strategies for mycotoxins control at the grain production level, a pilot study and exploratory research were conducted in 2019 and 2020 to compare levels of mycotoxins in grains of plants treated with two fertilizers, F-BAC and Nefusoil, under real agricultural environment. The 1650 grains selected from the 33 samples were assessed for the presence of both Fusarium species and mycotoxins. Only fumonisins and deoxynivalenol were detected. Fumonisin B1 ranged from 0 to 2808.4 µg/Kg, and fumonisin B2 from 0 to 1041.9 µg/Kg, while deoxynivalenol variated from 0 to 465.8 µg/Kg. Nefusoil showed to be promising in regard to fumonisin control. Concerning the control of fungal contamination rate and the diversity of Fusarium species, no significant differences were found between the two treatments in any of the years. However, a tendency for was observed Nefusoil of lower values, probably due to the guaranteed less stressful conditions to the Fusarium spp. present in the soil, which do not stimulate their fumonisins production.publishersversionpublishe