Maternal macronutrient and energy intakes in pregnancy and offspring intake at 10 y: exploring parental comparisons and prenatal effects1234

Background: High maternal dietary intakes in pregnancy may lead to increased fetal growth and program neuroendocrine pathways that result in greater appetite, energy intake, and adiposity in offspring later in life. Few prospective dietary studies have explored this relation

The homogeneity of the purified dialysable proteinase inhibitor from eggplant exocarp (Agricultural Chemistry)

ナスビ果皮より単離精製されたトリプシン・インヒビターの均一性をセフアデックスG-50によるゲルロ過やデイスクおよびSDSポリアクリルアミドゲル電気泳動によって, しらべた。このインヒビター標品は完全に均一な蛋白質であることが判明した。またこのインヒビターはゲルロ過, SDSポリアクリルアミドゲル電気泳動および沈降平衡法によって, 分子量約6000であることが明らかになったが, この値は, 植物由来のインヒビターとして最小のものである。The homogeneity of the protein-like dialysable proteinase inhibitor purified from eggplant exocarp was investigated by gel chromatography on Sephadex G-50 and by disc and SDS-polyacrylamide gel electrophoreses. These analyses showed this inhibitor preparation was homogeneous protein. The molecular weight of this inhibitor was estimated to be approximately 6000 by the methods of gel chromatography, SDS-polyacrylamide gel electrophoresis and equilibrium centrifugation

The identification of a non-dialyzable proteinase inhibitor with the dialyzable from eggplant exocarps (Agricultural Chemistry)

ナスビ外皮より非透析性のプロテアーゼインヒビターを精製した。塩析, 熱処理, DEAE-セファデックスおよびゲル漏過を用いたが, 最初の段階を除いては, 透析は行わず, 脱塩はすべてダイヤファルターを用いた。このようにして精製したインヒビターは, その分子量, トリプシンやキモトリプシンに対する挙動, またアミノ酸組成の点からみて, 透析性およびアセチル化セルロースを透析に用いて精製したインヒビターと全く同じものであった。そしてナスビ外皮には主要なインヒビターとしては一種類しか存在しないことが明らかとなった。しかし分子量6000のインヒビターが, 未精製のとき, 何故, 透析膜中に残存するかは依然として不明である。Non-dialyzable proteinase inhibtor was purified from eggplant exocarp. The salting out, heat-treatment, DEAE-Sephadex and gel chromatographies were used, and the ultrafiltration was used instead of dialysis except first purification step. The molecular weight, inhibition behaviour for proteinases and amino acid composition were the same as those of purified inhibitor by the other methods. These results show that the non-dialyzable and dialyzable inhibitors are identical and only one kind of inhibitor exists in eggplant exocarp as the main inhibitor. However, the reason why the inhibitor of molecular weight of 6000 in crude stage remains in dialyzing tube, is still unclear

Origin of the Spin-Orbital Liquid State in a Nearly J=0 Iridate Ba3ZnIr2O9

We show using detailed magnetic and thermodynamic studies and theoretical calculations that the ground state of Ba3ZnIr2O9 is a realization of a novel spin-orbital liquid state. Our results reveal that Ba3ZnIr2O9 with Ir5+ (5d(4)) ions and strong spin-orbit coupling (SOC) arrives very close to the elusive J = 0 state but each Ir ion still possesses a weak moment. Ab initio density functional calculations indicate that this moment is developed due to superexchange, mediated by a strong intradimer hopping mechanism. While the Ir spins within the structural Ir2O9 dimer are expected to form a spin-orbit singlet state (SOS) with no resultant moment, substantial frustration arising from interdimer exchange interactions induce quantum fluctuations in these possible SOS states favoring a spin-orbital liquid phase down to at least 100 mK

Vitamin C in plasma is inversely related to blood pressure and change in blood pressure during the previous year in young Black and White women

BackgroundThe prevalence of hypertension and its contribution to cardiovascular disease risk makes it imperative to identify factors that may help prevent this disorder. Extensive biological and biochemical data suggest that plasma ascorbic acid may be such a factor. In this study we examined the association between plasma ascorbic acid concentration and blood pressure (BP) in young-adult women.MethodsParticipants were 242 Black and White women aged 18-21 yr from the Richmond, CA, cohort of the National Heart, Lung and Blood Institute Growth and Health Study. We examined the associations of plasma ascorbic acid with BP at follow-up year 10, and with change in BP during the previous year.ResultsIn cross-sectional analysis, plasma ascorbic acid at year 10 was inversely associated with systolic BP and diastolic BP after adjusting for race, body mass index, education, and dietary intake of fat and sodium. Persons in the highest one-fourth of the plasma ascorbic acid distribution had 4.66 mmHg lower systolic BP (95% CI 1.10 to 8.22 mmHg, p = 0.005) and 6.04 mmHg lower diastolic BP (95% CI 2.70 to 9.38 mmHg, p = 0.0002) than those in the lowest one-fourth of the distribution. In analysis of the change in BP, plasma ascorbic acid was also inversely associated with change in systolic BP and diastolic BP during the previous year. While diastolic blood pressure among persons in the lowest quartile of plasma ascorbic acid increased by 5.97 mmHg (95% CI 3.82 to 8.13 mmHg) from year 9 to year 10, those in the highest quartile of plasma vitamin C increased by only 0.23 mmHg (95% CI -1.90 to +2.36 mmHg) (test for linear trend: p < 0.0001). A similar effect was seen for change in systolic BP, p = 0.005.ConclusionPlasma ascorbic acid was found to be inversely associated with BP and change in BP during the prior year. The findings suggest the possibility that vitamin C may influence BP in healthy young adults. Since lower BP in young adulthood may lead to lower BP and decreased incidence of age-associated vascular events in older adults, further investigation of treatment effects of vitamin C on BP regulation in young adults is warranted

Supporting Information: Exclusion lists for single lipids

We here provide examples in the form of input PDB files, resulting potential files and exclusion lists to generate a correct potential file usable with polarizable embedding in DALTON. We include three PDB files, each with a single lipid: POPC, POPS or DMPC. Based on these PDB files we have generated potential files (.pot) to be used in polarizable embedding calculations. In order to correctly account for polarization effects proper exclusion lists have to be generated. The exclusion lists for the PDB files are listed in the bottom of the potential files. In order to correctly generate the exclusion lists for a different order of atoms we have provided named atom lists (.txt files). This Figshare project is for the work at the DOI: 10.1002/jcc.2471

2BVZ : Mutant of the Ribosomal Protein S6

Experimental Technique/Method:X-RAY DIFFRACTION Resolution:2.2 Classification:RIBOSOMAL PROTEIN Release Date:2005-10-26 Deposition Date:2005-07-05 Revision Date:2011-05-08#2011-07-13 Molecular Weight:11946.67 Macromolecule Type:Protein Residue Count:101 Atom Site Count:814 DOI:10.2210/pdb2bvz/pdb Abstract: When folding to the native state N in the presence of salt, the apparent two-state folder S6 transiently forms a transient off-pathway state C with substantial secondary and tertiary structure. Fifteen double mutant cycles were analysed to compare side-chain interaction energies DeltaDeltaG(int) in C, N and TS (the transition state between N and the denatured state). The kinetic signatures of these destabilizing mutants suggest folding scenarios involving unfolding intermediates and even alternative unfolding pathways. However, restricting the kinetic data to linear parts of the chevron plot allows reliable extrapolation to zero molar denaturant of rate constants of folding, unfolding and misfolding. Side-chain interactions appear to contribute to the stability of C, but in a substantially non-native environment, as shown by changes in the sign of DeltaDeltaG(int) between C and N. Remarkably, there appear to be significant (0.7-2 kcal/mol) antagonistic interactions between the two residues Leu30 and Leu75 in N and TS, which may be linked to subtle structural changes seen in the crystal structures of the mutants. A small number of overlapping residues are involved in these kinds of antagonistic interactions in N, TS and C, suggesting that repulsive interactions are coded into the protein topology whether the protein folds or misfolds. Destabilizing double mutants indicate that apparent two-state folders can be induced to behave in more complex ways provided that the native state is suitably destabilized