Clinical, Hematological, Blood Gasometric and Electrolytic Changes in Dogs Receiving Whole Blood Transfusions

Background: Blood transfusion is a tool capable of saving lives. Patients undergoing blood transfusion usually present several alterations in the acid-base and electrolyte balance, aggravating the condition of critically ill patients. Some studies have demonstrated haematological alterations in certain species that received whole blood transfusions, however, few studies have evaluated acid base and electrolyte changes in dogs undergoing whole blood haemotherapy. The aim of this study was to analyze clinical, hematological, blood gas and electrolyte changes in anemic dogs after whole blood transfusion.Materials, Methods & Results:Twenty nine dogs transfused due to anemia were enrolled in the study. Donors blood was collected in a transfusion bag containing citrate phosphate adenine dextrose and stored up to 24 h. Blood collections and evaluations were made before and 24 h after the transfusion. Data distribution normality was tested by the Shapiro Wilk Test. The means of the variables were compared by paired t-test. It was observed an increase in diastolic blood pressure and a reduction in heart rate (P 0.05) and ionized calcium (P > 0.05) and an increase in the mean partial pressure of carbon dioxide (pCO2) (P 0.05) and sodium (P < 0.05). The probable cause of anemia was monocytic ehrlichiosis (14/29), visceral leishmaniasis (1/29), babesiosis (1/29), co-infection of Ehrlichia canis and Leishmaniainfantum (2/19), co-infection of E. canis and Babesia vogeli (1/19). It was not possible to determine the etiology of the anemia in ten dogs. Discussion:Heart rate significantly reduced after transfusion, probably because of the increase in hematocrit, hemoglobin and erythrocyte values. It may be justified by the displacement of extravascular fluid to the intravascular space. Mean values of systolic blood pressure were slightly elevated before transfusion and remained elevated afterwards, while diastolic and mean arterial pressure increased significantly after transfusion. These changes may be due to the morbid condition and may be influenced by many other factors. Haematocrit, haemoglobin and erythrocyte values increased significantly after transfusion, according to what was observed in other studies. The significant reduction in pH and increase in pCO2 reflects the compensatory mechanism for metabolic acidosis to increase ventilation, leading to pCO2 reduction and changes in pH. The reduction in pH due to the contact of the collected blood with conservative solutions is one of the main changes thar occurs during blood storage. It was described significantly lower pH in dogs’ whole blood samples stored for more than 24h in vacutainer plastic containing CPDA-1. We may assume there was no intense pH reduction in the present study because the bags were stored for up to 24 h. Although not statistically significant, the increase of pO2 mean reflects the improvement of tissue oxygen perfusion. It was observed a significant increase in sodium ions. The mean sodium ionconcentration before transfusion was very close to the maximum reference value. Hyperkalaemia was not observed, nor was there significant reduction of potassium ions after transfusion. Several studies report hyperkalaemia and transfusion-associated cardiac arrests in humans, associated with infusion of large volumes of blood. Whole blood transfusion increased erythrogram values and did not negatively affect the electrolyte or acid-base status, representing a safe and useful tool in the intensive care of small animals.

Vacuna emocional en la Covid-19: programa de autocuidado para personas mayores

The group of elderly people is one of the most affected by this pandemic. It is considered a risk group and age is a relevant factor in the body’s response to COVID-19. To face this situation and disease, it is important that people take care of themselves at a biopsychosocial level. The aim of this paper is to present a self-care and psychological activation program: “Emotional Vaccine for COVID-19”. It is aimed at elderly people without or with mild cognitive impairment, individually or in groups, in different environments: residential, home, day center, etc., assessing the effect of this program in the improvement of their quality of life, distinguishing between rural and urban contexts. The program consists of 10 sessions of one hour and a half, held twice a week. In each session, a self-care guideline is worked on through a message in the form of a saying that facilitates mnesic activation. From the Acceptance and Commitment Therapy and following the paradigm of the Attention Centered on what is Important for the Person, emotional management, cognitive stimulation and behavioral activation are addressed in order to produce better coping strategies for the current situation. The life project of each participant is emphasized through orientation to values. Using a quasi-experimental pre-post design with a control group, the following instruments are used: behavioral records, scales and questionnaires that measure quality of life, anxiety, depression and experiential avoidance. An improvement in health and well-being through psychological activation is expected in the target sample with respect to the control group. The need for this type of psychological intervention in the gerontological field is highlighted due to its feasibility, efficacy and social demand.El colectivo de personas mayores es uno de los más afectados por esta pandemia. Es considerado grupo de riesgo y la edad es un factor relevante en la respuesta que da el organismo frente a la Covid-19. Para afrontar esta situación y esta enfermedad es importante que las personas se cuiden a nivel biopsicosocial. El presente trabajo tiene como fin exponer un programa de autocuidado y de activación psicológica: “Vacuna Emocional para la COVID-19”. Dirigido a personas mayores sin o con deterioro cognitivo leve, de manera individual o grupal, en diferentes entornos: residencial, domiciliario, centro de día, etc., valorando el efecto de este programa en la mejora de su calidad de vida, distinguiendo entre contextos rurales y urbanos. El programa consta de 10 sesiones de una hora y media de duración aplicándose dos sesiones semanales. En cada sesión se trabaja una pauta de autocuidado a través de un mensaje con forma de refrán que facilita la activación mnésica. Desde la Terapia de Aceptación y Compromiso y siguiendo el paradigma de la Atención Centrada en lo Importante para la Persona, se aborda la gestión emocional, la estimulación cognitiva y la activación conductual de manera que se produzca un mejor afrontamiento de la situación actual. Se enfatiza el proyecto de vida de cada participante a través de la orientación en valores. Utilizando un diseño cuasiexperimental pre-post con grupo control, se emplean los instrumentos: registros conductuales, escalas y cuestionarios que midan calidad de vida, ansiedad, depresión y evitación experiencial. Se espera una mejora en la salud y bienestar a través de la activación psicológica en la muestra diana respecto al grupo control. Se destaca la necesidad de este tipo de intervención psicológica en el ámbito gerontológico por su viabilidad, eficacia y demanda social.

OSTEOMIELITE CAUSADA POR Escherichia coli E Bacillus spp. EM PAPAGAIO VERDADEIRO - RELATO DE CASO

The objective is to describe a case of osteomyelitis of a Blue-fronted Parrot (Amazona aestiva) with fractured left pelvic member accompanied of osteolysis, which was attended at the Veterinary Hospital of the Federal University of Mato Grosso, Campus Sinop-MT. After clinical examination, radiographic, bacterial culture and antibiotic susceptibility testing, was confirmed the diagnosis of osteomyelitis caused by Escherichia coli and Bacillus spp. Osteomyelitis is an inflammatory process accompanied by bone destruction caused by an infectious microorganism, can be found in acute and chronic forms. The diagnosis of osteomyelitis depends not only on radiographic findings but other tests such as culture and antibiotic susceptibility testing. In this case Escherichia coli was sensitive to all antimicrobial used, but Bacillus spp. was resistant to some of antibacterials used: Enrofloxacin, Ampicillin, Chloramphenicol, Tetracycline, Norfloxacin and Ciprofloxacin. The treatment chosen for the case was amputation of the affected limb. This is the first description of the occurrence of osteomyelitis caused by the bacterium Escherichia coli and Bacillus spp. in a Blue-fronted Parrot in the state of Mato Grosso, Brazil.O objetivo do presente trabalho foi descrever um caso de osteomielite em papagaio verdadeiro (Amazona aestiva,) com fratura no membro pélvico esquerdo, acompanhada de osteólise, que foi atendido no Hospital Veterinário da Universidade Federal de Mato Grosso, Campus Sinop-MT. Após exames clínico, radiográficos, cultura bacteriana e antibiograma, confirmou-se o diagnóstico de osteomielite, causada pelas bactérias Escherichia coli e Bacillus spp. A osteomielite é um processo inflamatório, acompanhado de destruição óssea, causada por um microrganismo infectante e que pode ser encontrada nas formas aguda e crônica. O diagnóstico da osteomielite depende, não só de achados radiográficos, mas de outros exames como cultura e antibiograma. No presente caso, Escherichia coli mostrou-se sensível a todos os antibacterianos utilizados; mas Bacillus spp. mostrou-se resistente a alguns dos antibacterianos utilizados: Enrofloxacina, Ampicilina, Cloranfenicol, Tetraciclina, Norfloxacin e Ciprofloxacina. O tratamento escolhido para o caso foi amputação do membro acometido. Este é a primeira descrição da ocorrência de osteomielite causada pela bactéria Escherichia coli e Bacillus spp. em um papagaio verdadeiro no estado do Mato Grosso