On the occurrence of the Bull Ray Pteromylaeus bovinus (Chondrichthyes: Myliobatidae) in the Amvrakikos Gulf, Greece

We document in this note the occurrence of the Bull Ray Pteromylaeus bovinus (Geoffroy St-Hilaire, 1817) (Chondrichthyes: Myliobatidae) in the Amvrakikos Gulf (eastern Ionian Sea), within a recently designated Wetlands National Park. Although distributional data for this species are scant, there is circumstantial evidence depicting the species as rare in Greece and the Mediterranean

First record of Pontian Monkey Goby, Neogobius fluviatilis (Pallas, 1814) in the Evros River (Greece); Is it an alien species?

The Pontian Monkey Goby,Neogobius fluviatilis (Pallas, 1814), was recorded for the first time in Greece in August 2011. Eight specimens were collected in the Greek-Turkish section of the Evros River, 65 kilometers upstream of its river-mouth. Although the species has been recently discovered in the Tundza, a tributary of the Evros in Bulgaria, it has never before been found in the Evros’ main stem.  Although the lower Evros has been poorly researched by ichthyologists, it is unlikely that a conspicuous medium-sized fish would go unnoticed in this river; and, it is therefore suggested to be a probable alien. However, since the Evros basin has had geological connections to the Marmara Sea and Black Sea in the past and it is immediately adjacent to native populations of N. fluviatilis, the species status is categorized as questionable until genetic and morphological studies are completed

Riparian woodland flora in upland rivers of Western Greece

Although natural riparian woodlands are an important feature that affects the quality of aquatic conditionsin streams and rivers, surveying riparian zone flora is rarely implemented in the Mediterraneancountries. We developed a rapid assessment method for gathering standardized plot-based woody flora andvegetation data from riparian woodlands. In 2005 we surveyed 218 streamside vegetation plots at 109 sitesin upland areas of four major rivers in mainland Greece (Alfios, Acheloos, Arachthos, and Aoos). Herewe describe the survey method and provide selected results from its initial implementation. The simplicityand effectiveness of this survey procedure supports the use of rapid site-based biodiversity surveys for riparianzones alongside aquatic status assessments

First record of the Tadpole Shrimp Triops cancriformis (Branchiopoda, Notostraca) in Cyprus

The presence of the large branchiopod Triops cancriformis (Bosc, 1801-1802) in Cyprus is reported for the first time. Triops cancriformis individuals were observed in the large temporary wetland of Paralimni Lake (Famagusta District, Cyprus) during the wet periods of 2013 and 2014. This finding extends the current knowledge of the species distribution in the eastern Mediterranean area, and stresses the ecological and conservation value of the unique habitat of Paralimni Lake. In addition, it highlights the need for carrying on further studies on the fauna of temporary pond habitats in Cyprus, and for better conservation management strategies for such EU priority habitats

Developing policy-relevant river fish monitoring in Greece: Insights from a nation-wide survey

A wide-ranging river fish survey was executed in the summer of 2009 as part of the preparatory actions for the establishment of a monitoring programme for the EU Water Framework Directive (WFD). This was the first extensive electrofishing campaign for WFD standardized bioassessment in Greece and the experience and insights gained are used here to provide a review of fish-based assessment conditions and requirements in this country. The survey sampled 85 sites on 25 rivers throughout mainland Greece, collecting 70 species of freshwater fish. Quantitative site-based assemblage data is used for taxonomic and ordination analyses revealing a strong biogeographic regionalization in the distribution of the ichthyofauna. The structural and spatial organisation of the fish fauna through the use of species-level and community-level data analyses is explored in three ecoregions where data was deemed sufficient. Transitions in community taxonomic composition among ecoregions were abrupt and concordant with geographical barriers and reflect the influence of historical biogeographic processes. Community-based analysis revealed a substantial degree of variation in quantitative attributes of the fish assemblages among ecoregions. Key conclusions of this work are: (a) the fish-based bioassessment system must be regionalised to reflect biogeographic variation, (b) high faunal heterogeneity among ecoregions (taxonomic, structural), and to a lower degree among basins, constrain the transferability of bioassessment metrics and indices created for explicit regions to other regional frameworks; (c) faunal depauperation in most of the study areas reduce the utility of functional bioassessment metrics and also limits the utilization of rare species and the applicability of the classical form of the “Index of Biotic Integrity” concept. Recommendations to cope with these problems are discussed

Recent contributions to the distribution of the freshwater ichthyofauna in Greece

In this paper we supplement Greece’s recent annotated inventory of freshwater fishes per hydrographic basin with recent distributional data and taxa alteration information, based on field sampling and a literature review up to September 2011. We report on newly documented distributional records of 31 fish species plus one unidentified taxon, within 35 hydrographic river basin units in Greece. These new records include 14 native fish species, seven alien and 12 translocated. Translocated taxa are distinguished from aliens, in order to report species non-indigenous to a basin but native within the same ecoregion. Twelve hydrographic basin units are newly added to the roster of ichthyologically explored river basins following a previous basin-scale inventory method (the total is now 117). This review increases the number of Greece’s freshwater fish taxa to 167, since four new species are added to the list (Carassius langsdorfii, Neogobius fluviatilis, Telestes alfiensis, Millerigobius macrocephalus) and two are deleted (Salmo dentex, Barbus rebeli) due to taxonomic changes. Taxonomic changes will probably continue to alter the national list since phylogenetic research is ongoing on several taxa in many parts of the countr

The freshwater ichthyofauna of Greece - an update based on a hydrographic basin survey

Distribution records (historical, contemporary) for native and non-native freshwater fish species from 105 hydrographic basin areas were compiled and analysed in order to develop a nation-wide inventory (including transboundary river basins). Overall, 162 species, including diadromous and euryhaline, with documented occurrence records in freshwaters, and taxa of unclarified taxonomic status, are accommodated in the distributional compilation. An annotated checklist summarises the confirmed ichthyofauna of Greek freshwaters (161 species); a provisional supplementary list contains species recorded in brackish waters (55 species). In comparison to the last published (1991) checklist of freshwater fish of Greece, the present checklist shows an increase in species number of 53% (56 species). This increase has resulted mainly from taxonomic re-evaluations of existing taxa on the basis of new information and adoption of a new systematic concept. The current trend, as reflected in recent ichthyological publications, is towards abandonment of the biological species concept (BSC) and adoption of the phylogenetic species concept (PSC) for the delineation of species boundaries. The practical implications of the change in species concept on biodiversity conservation and watershed management are discussed. An overview of the composition and characteristics of the freshwater fish fauna of Greece is provided, especially with regard to the native and introduced status of species, and the spatial patterns of species richness and endemism. This systematic inventory may assist in efforts to develop nation-wide surface water bioassessment tools within the demands of the Water Framework Directive (WFD); it may further promote biodiversity conservation and biologically-orientated fishery management approaches

Generalized additive and fuzzy models in environmental flow assessment: A comparison employing the West Balkan trout (Salmo farioides; Karaman, 1938)

Human activities have altered flow regimes resulting in increased pressures and threats on river biota. Physical habitat simulation has been established as a standard approach among the methods for Environmental Flow Assessment (EFA). Traditionally, in EFA, univariate habitat suitability curves have been used to evaluate the habitat suitability at the microhabitat scale whereas Generalized Additive Models (GAMs) and fuzzy logic are considered the most common multivariate approaches to do so. The assessment of the habitat suitability for three size classes of the West Balkan trout (Salmo farioides; Karaman, 1938) inferred with these multivariate approaches was compared at three different levels. First the modelled patterns of habitat selection were compared by developing partial dependence plots. Then, the habitat assessment was spatially explicitly compared by calculating the fuzzy kappa statistic and finally, the habitat quantity and quality was compared broadly and at relevant flows under a hypothetical flow regulation, based on the Weighted Usable Area (WUA) vs. flow curves. The GAMs were slightly more accurate and the WUA-flow curves demonstrated that they were more optimistic in the habitat assessment with larger areas assessed with low to intermediate suitability (0.2 0.6). Nevertheless, both approaches coincided in the habitat assessment (the optimal areas were spatially coincident) and in the modelled patterns of habitat selection; large trout selected microhabitats with low flow velocity, large depth, coarse substrate and abundant cover. Medium sized trout selected microhabitats with low flow velocity, middle-to-large depth, any kind of substrate but bedrock and some elements of cover. Finally small trout selected microhabitats with low flow velocity, small depth, and light cover only avoiding bedrock substrate. Furthermore, both approaches also rendered similar WUA-flow curves and coincided in the predicted increases and decreases of the WUA under the hypothetical flow regulation. Although on an equal footing, GAMs performed slightly better, they do not automatically account for variables interactions. Conversely, fuzzy models do so and can be easily modified by experts to include new insights or to cover a wider range of environmental conditions. Therefore, as a consequence of the agreement between both approaches, we would advocate for combinations of GAMs and fuzzy models in fish-based EFA.This study was supported by the ECOFLOW project funded by the Hellenic General Secretariat of Research and Technology in the framework of the NSRF 2007-2013. We are grateful for field assistance of Dimitris Kommatas, Orfeas Triantafillou and Martin Palt and to Alcibiades N. Economou for assistance in discussions on trout biology and ecology.Muñoz Mas, R.; Papadaki, C.; Martinez-Capel, F.; Zogaris, S.; Ntoanidis, L.; Dimitriou, E. (2016). Generalized additive and fuzzy models in environmental flow assessment: A comparison employing the West Balkan trout (Salmo farioides; Karaman, 1938). Ecological Engineering. 91:365-377. doi:10.1016/j.ecoleng.2016.03.009S3653779

From sea monsters to charismatic megafauna: changes in perception and use of large marine animals

Marine megafauna has always elicited contrasting feelings. In the past, large marine animals were often depicted as fantastic mythological creatures and dangerous monsters, while also arousing human curiosity. Marine megafauna has been a valuable resource to exploit, leading to the collapse of populations and local extinctions. In addition, some species have been perceived as competitors of fishers for marine resources and were often actively culled. Since the 1970s, there has been a change in the perception and use of megafauna. The growth of marine tourism, increasingly oriented towards the observation of wildlife, has driven a shift from extractive to non-extractive use, supporting the conservation of at least some species of marine megafauna. In this paper, we review and compare the changes in the perception and use of three megafaunal groups, cetaceans, elasmobranchs and groupers, with a special focus on European cultures. We highlight the main drivers and the timing of these changes, compare different taxonomic groups and species, and highlight the implications for management and conservation. One of the main drivers of the shift in perception, shared by all the three groups of megafauna, has been a general increase in curiosity towards wildlife, stimulated inter alia by documentaries (from the early 1970s onwards), and also promoted by easy access to scuba diving. At the same time, environmental campaigns have been developed to raise public awareness regarding marine wildlife, especially cetaceans, a process greatly facilitated by the rise of Internet and the World Wide Web. Currently, all the three groups (cetaceans, elasmobranchs and groupers) may represent valuable resources for ecotourism. Strikingly, the economic value of live specimens may exceed their value for human consumption. A further change in perception involving all the three groups is related to a growing understanding and appreciation of their key ecological role. The shift from extractive to non-extractive use has the potential for promoting species conservation and local economic growth. However, the change in use may not benefit the original stakeholders (e.g. fishers or whalers) and there may therefore be a case for providing compensation for disadvantaged stakeholders. Moreover, it is increasingly clear that even non-extractive use may have a negative impact on marine megafauna, therefore regulations are needed.SFRH/BPD/102494/2014, UID/MAR/04292/2019, IS1403info:eu-repo/semantics/publishedVersio

Integrative phylogenetic, phylogeographic and morphological characterisation of the Unio crassus species complex reveals cryptic diversity with important conservation implications

The global decline of freshwater mussels and their crucial ecological services highlight the need to understand their phylogeny, phylogeography and patterns of genetic diversity to guide conservation efforts. Such knowledge is urgently needed for Unio crassus, a highly imperilled species originally widespread throughout Europe and southwest Asia. Recent studies have resurrected several species from synonymy based on mitochondrial data, revealing U. crassus to be a complex of cryptic species. To address long-standing taxonomic uncertainties hindering effective conservation, we integrate morphometric, phylogenetic, and phylogeographic analyses to examine species diversity within the U. crassus complex across its entire range. Phylogenetic analyses were performed using cytochrome c oxidase subunit I (815 specimens from 182 populations) and, for selected specimens, whole mitogenome sequences and Anchored Hybrid Enrichment (AHE) data on ∼600 nuclear loci. Mito-nuclear discordance was detected, consistent with mitochondrial DNA gene flow between some species during the Pliocene and Pleistocene. Fossil-calibrated phylogenies based on AHE data support a Mediterranean origin for the U. crassus complex in the Early Miocene. The results of our integrative approach support 12 species in the group: the previously recognised Unio bruguierianus, Unio carneus, Unio crassus, Unio damascensis, Unio ionicus, Unio sesirmensis, and Unio tumidiformis, and the reinstatement of five nominal taxa: Unio desectus stat. rev., Unio gontierii stat. rev., Unio mardinensis stat. rev., Unio nanus stat. rev., and Unio vicarius stat. rev. Morphometric analyses of shell contours reveal important morphospace overlaps among these species, highlighting cryptic, but geographically structured, diversity. The distribution, taxonomy, phylogeography, and conservation of each species are succinctly described