Digestion of Protein in Premature and Term Infants.

Premature birth rates and premature infant morbidity remain discouragingly high. Improving nourishment for these infants is the key for accelerating their development and decreasing disease risk. Dietary protein is essential for growth and development of infants. Studies on protein nourishment for premature infants have focused on protein requirements for catch-up growth, nitrogen balance, and digestive protease concentrations and activities. However, little is known about the processes and products of protein digestion in the premature infant. This review briefly summarizes the protein requirements of term and preterm infants, and the protein content of milk from women delivering preterm and at term. An in-depth review is presented of the current knowledge of term and preterm infant dietary protein digestion, including human milk protease and anti-protease concentrations; neonatal intestinal pH, and enzyme activities and concentrations; and protein fermentation by intestinal bacteria. The advantages and disadvantages of incomplete protein digestion as well as factors that increase resistance to proteolysis of particular proteins are discussed. In order to better understand protein digestion in preterm and term infants, future studies should examine protein and peptide fragment products of digestion in saliva, gastric, intestinal and fecal samples, as well as the effects of the gut micro biome on protein degradation. The confluence of new mass spectrometry technology and new bioinformatics programs will now allow thorough identification of the array of peptides produced in the infant as they are digested

Background modeling for video sequences by stacked denoising autoencoders

Nowadays, the analysis and extraction of relevant information in visual data flows is of paramount importance. These images sequences can last for hours, which implies that the model must adapt to all kinds of circumstances so that the performance of the system does not decay over time. In this paper we propose a methodology for background modeling and foreground detection, whose main characteristic is its robustness against stationary noise. Thus, stacked denoising autoencoders are applied to generate a set of robust characteristics for each region or patch of the image, which will be the input of a probabilistic model to determine if that region is background or foreground. The evaluation of a set of heterogeneous sequences results in that, although our proposal is similar to the classical methods existing in the literature, the inclusion of noise in these sequences causes drastic performance drops in the competing methods, while in our case the performance stays or falls slightly.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Dysfunctional telomeres in primary cells from Fanconi anemia FANCD2 patients

© 2012 Joksic et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.This article has been made available through the Brunel Open Access Publishing Fund.Background: Fanconi anemia (FA) is characterized by sensitivity to DNA cross-linking agents, mild cellular, and marked clinical radio sensitivity. In this study we investigated telomeric abnormalities of non-immortalized primary cells (lymphocytes and fibroblasts) derived from FA patients of the FA-D2 complementation group, which provides a more accurate physiological assessment than is possible with transformed cells or animal models. Results: We analyzed telomere length, telomere dysfunction-induced foci (TIFs), sister chromatid exchanges (SCE), telomere sister chromatid exchanges (T-SCE), apoptosis and expression of shelterin components TRF1 and TRF2. FANCD2 lymphocytes exhibited multiple types of telomeric abnormalities, including premature telomere shortening, increase in telomeric recombination and aberrant telomeric structures ranging from fragile to long-string extended telomeres. The baseline incidence of SCE in FANCD2 lymphocytes was reduced when compared to control, but in response to diepoxybutane (DEB) the 2-fold higher rate of SCE was observed. In contrast, control lymphocytes showed decreased SCE incidence in response to DEB treatment. FANCD2 fibroblasts revealed a high percentage of TIFs, decreased expression of TRF1 and invariable expression of TRF2. The percentage of TIFs inversely correlated with telomere length, emphasizing that telomere shortening is the major reason for the loss of telomere capping function. Upon irradiation, a significant decrease of TIFs was observed at all recovery times. Surprisingly, a considerable percentage of TIF positive cells disappeared at the same time when incidence of γ-H2AX foci was maximal. Both FANCD2 leucocytes and fibroblasts appeared to die spontaneously at higher rate than control. This trend was more evident upon irradiation; the percentage of leucocytes underwent apoptosis was 2.59- fold higher than that in control, while fibroblasts exhibited a 2- h delay before entering apoptosis. Conclusion: The results of our study showed that primary cells originating from FA-D2 patients display shorten telomeres, elevated incidence of T-SCEs and high frequency of TIFs. Disappearance of TIFs in early response to irradiation represent distinctive feature of FANCD2 cells that should be examined further.This article is made available through the Brunel Open Access Publishing Fund. This work was supported by the Ministry of Education and Science of the Republic of Serbia (Project No.173046)

Flow regime map of a liquid-solid micro-circulating fluidized bed

Solid-liquid micro-fluidised beds (FBs), which are essentially fluidisation of micro-particles in sub-centimetre beds, hold promise of applications in the microfluidics and micro-process technology context. This is mainly due to fluidised particles providing enhancement of mixing, mass and heat transfer under the low Reynolds number flows that dominate in micro-devices. Sometimes particle circulations is required or desirable (e.g. continuous regeneration of a catalyst) for which circulating fluidised beds are ideal, not to mention advantages of better interfacial contacting and reduced backmixing compared to a classical fluidised bed (1). Albeit there are quite a few studies of solid-liquid micro-fluidised beds, we are presenting the first study of micro-circulating fluidised bed. A transparent micro-circulating fluidised bed was made by micro-machining channels of 1 mm2 square cross section in Perspex as shown in Fig. 1. We used soda-lime glass microparticles and tap water as fluidising medium to study flow regime transition for this micro-circulating fluidised bed. The flow regime map as proposed by Liang et al. (2) was produced for a micro-circulating fluidised bed and is given in Fig. 2. Essentially results are almost the same as for the macroscopic counterparts with the transition to the circulating fluidised regime occurring at velocities, Ucr, slightly above the particle terminal velocities, Ut (1,2). The reported critical transition velocity is for high enough solid inventories (above 10% of the system volume) where this transition velocity is constant, while for lower solid inventories it is bigger as in previous experiments (1,2). While the minimum fluidization velocity, Umf, is influenced by adhesion forces and wall effects (3), there is a weak increase in the normalized critical transition velocity for circulating regime, Ucr/Ut, with an increase of particle diameter (not shown here). This may be due to the wall effects but more studies are needed to elucidate this further. Please click Additional Files below to see the full abstract

Strength Analysis of Eight-Wheel Bogie of Bucket Wheel Excavator

Crawler travel gear is a type of heavy vehicle propulsion that is commonly found in tanks, excavators, and specialized off-road vehicles. They have an advantage over wheels when it comes to robust vehicle weight distribution over soft terrain, and some disadvantages as well. They can damage paved roads and have complex design so, considering the enormous weight they must carry, their reliability must be determined and verified. The main parts of the assembly are the drive wheels, which move the crawler, and the supporting structure that holds four-wheel bogies and two-wheel bogies. In this paper, we present a methodology for FEM analysis of parts of an eight-wheel bogie according to DIN 22261-2 standard

Background modeling by shifted tilings of stacked denoising autoencoders

The effective processing of visual data without interruption is currently of supreme importance. For that purpose, the analysis system must adapt to events that may affect the data quality and maintain its performance level over time. A methodology for background modeling and foreground detection, whose main characteristic is its robustness against stationary noise, is presented in the paper. The system is based on a stacked denoising autoencoder which extracts a set of significant features for each patch of several shifted tilings of the video frame. A probabilistic model for each patch is learned. The distinct patches which include a particular pixel are considered for that pixel classification. The experiments show that classical methods existing in the literature experience drastic performance drops when noise is present in the video sequences, whereas the proposed one seems to be slightly affected. This fact corroborates the idea of robustness of our proposal, in addition to its usefulness for the processing and analysis of continuous data during uninterrupted periods of time.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Tolerability and safety of the intake of bovine milk oligosaccharides extracted from cheese whey in healthy human adults.

Mechanistic research suggests a unique evolutionary relationship between complex milk oligosaccharides and cognate bifidobacteria enriched in breast-fed infants. Bovine milk oligosaccharides (BMO) were recently identified as structurally and functionally similar to human milk oligosaccharides. The present single-blind three-way crossover study is the first to determine the safety and tolerability of BMO consumption by healthy human participants (n 12) and its effects on faecal microbiota and microbial metabolism. Participants consumed each supplement (placebo-control; low- and high-BMO doses) for eleven consecutive days, followed by a 2-week washout period prior to initiating the next supplement arm. Low and high BMO doses were consumed as 25 and 35 % of each individual's daily fibre intake, respectively. Safety and tolerability were measured using standardised questionnaires on gut and stomach discomfort and stool consistency. Faecal extracts were profiled for bacterial populations by next-generation sequencing (NGS) and bifidobacteria presence was confirmed using quantitative PCR. Urine was analysed for changes in microbial metabolism using nuclear magnetic resonance spectroscopy (1H-NMR). Consumption of both the low and high BMO doses was well tolerated and did not change stool consistency from baseline. Multivariate analysis of the NGS results demonstrated no change in faecal microbiota phyla among the placebo-control and BMO supplement groups. In conclusion, BMO supplementation was well tolerated in healthy adults and has the potential to shift faecal microbiota toward beneficial strains as part of a synbiotic treatment with probiotic cultures that selectively metabolise oligosaccharides

Doping Dependence of the in-Plane Transition in Co3_3Sn2_2S2_2

In Co$_3$Sn$_2$S$_2$ two transitions are observed, the main one to a ferromagnetic state at $T_C = 174$ K and the second one, involving in-plane components at $T_P = 127$ K. We follow their doping dependence as Sn is replaced with In, which causes a reduction of $T_C$ and $T_P$. Importantly, both transitions follow the same doping dependence, indicating a single energy scale involved with both processes.Comment: accepted as a short note in JPSJ; a continuation of work published in arXiv:2211.01483 and https://doi.org/10.1103/PhysRevB.106.L18040