Simultaneous whole-animal 3D-imaging of neuronal activity using light field microscopy

3D functional imaging of neuronal activity in entire organisms at single cell level and physiologically relevant time scales faces major obstacles due to trade-offs between the size of the imaged volumes, and spatial and temporal resolution. Here, using light-field microscopy in combination with 3D deconvolution, we demonstrate intrinsically simultaneous volumetric functional imaging of neuronal population activity at single neuron resolution for an entire organism, the nematode Caenorhabditis elegans. The simplicity of our technique and possibility of the integration into epi-fluoresence microscopes makes it an attractive tool for high-speed volumetric calcium imaging.Comment: 25 pages, 7 figures, incl. supplementary informatio

Feeding spectra and activity of the freshwater crab Trichodactylus kensleyi (Decapoda: Brachyura: Trichodactylidae) at La Plata basin

Background: In inland water systems, it is important to characterize the trophic links in order to identify the ‘trophic species’ and, from the studies of functional diversity, understand the dynamics of matter and energy in these environments. The aim of this study is to analyze the natural diet of Trichodactylus kensleyi of subtropical rainforest streams and corroborate the temporal variation in the trophic activity during day hours. Results: A total of 15 major taxonomic groups were recognized in gut contents. The index of relative importance identified the following main prey items in decreasing order of importance: vegetal remains, oligochaetes, chironomid larvae, and algae. A significant difference was found in the amount of full stomachs during day hours showing a less trophic activity at midday and afternoon. The index of relative importance values evidenced the consumption of different prey according to day moments. Results of the gut content indicate that T. kensleyi is an omnivorous crab like other trichodactylid species. Opportunistic behavior is revealed by the ingestion of organisms abundant in streams such as oligochaetes and chironomid larvae. The consumption of allochthonous plant debris shows the importance of this crab as shredder in subtropical streams. However, the effective assimilation of plant matter is yet unknown in trichodactylid crabs. Conclusions: This research provides knowledge that complements previous studies about trophic relationships of trichodactylid crabs and supported the importance of T. kensleyi in the transference of energy and matter from benthic community and riparian sources to superior trophic levels using both macro- and microfauna.Fil: Williner, Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias; ArgentinaFil: de Azevedo Carvalho, Debora. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; ArgentinaFil: Collins, Pablo Agustin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentin

Preserving the impossible: conservation of soft-sediment hominin footprint sites and strategies for three-dimensional digital data capture.

Human footprints provide some of the most publically emotive and tangible evidence of our ancestors. To the scientific community they provide evidence of stature, presence, behaviour and in the case of early hominins potential evidence with respect to the evolution of gait. While rare in the geological record the number of footprint sites has increased in recent years along with the analytical tools available for their study. Many of these sites are at risk from rapid erosion, including the Ileret footprints in northern Kenya which are second only in age to those at Laetoli (Tanzania). Unlithified, soft-sediment footprint sites such these pose a significant geoconservation challenge. In the first part of this paper conservation and preservation options are explored leading to the conclusion that to 'record and digitally rescue' provides the only viable approach. Key to such strategies is the increasing availability of three-dimensional data capture either via optical laser scanning and/or digital photogrammetry. Within the discipline there is a developing schism between those that favour one approach over the other and a requirement from geoconservationists and the scientific community for some form of objective appraisal of these alternatives is necessary. Consequently in the second part of this paper we evaluate these alternative approaches and the role they can play in a 'record and digitally rescue' conservation strategy. Using modern footprint data, digital models created via optical laser scanning are compared to those generated by state-of-the-art photogrammetry. Both methods give comparable although subtly different results. This data is evaluated alongside a review of field deployment issues to provide guidance to the community with respect to the factors which need to be considered in digital conservation of human/hominin footprints

TGF-beta(2)- and H2O2-Induced Biological Changes in Optic Nerve Head Astrocytes Are Reduced by the Antioxidant Alpha-Lipoic Acid

Background/Aims: The goal of the present study was to determine whether transforming growth factor-beta(2) (TGF-beta(2))- and oxidative stress-induced cellular changes in cultured human optic nerve head (ONH) astrocytes could be reduced by pretreatment with the antioxidant alpha-lipoic acid (LA). Methods: Cultured ONH astrocytes were treated with 1.0 ng/ml TGF-beta(2) for 24 h or 200 mu M hydrogen peroxide (H2O2) for 1 h. Lipid peroxidation was measured by a decrease in cis-pari-naric acid fluorescence. Additionally, cells were pretreated with different concentrations of LA before TGF-beta 2 or H2O2 exposure. Expressions of the heat shock protein (Hsp) alpha B-crystallin and Hsp27, the extracellular matrix (ECM) component fibronectin and the ECM-modulating protein connective tissue growth factor (CTGF) were examined with immunohistochemistry and real-time PCR analysis. Results: Both TGF-beta(2) and H2O2 increased lipid peroxidation. Treatment of astrocytes with TGF-beta(2) and H2O2 upregulated the expression of alpha B-crystallin, Hsp27, fibronectin and CTGF. Pretreatment with different concentrations of LA reduced the TGF-beta(2)- and H2O2-stimulated gene expressions. Conclusion: We showed that TGF-beta(2)- and H2O2-stimulated gene expressions could be prevented by pretreatment with the antioxidant LA in cultured human ONH astrocytes. Therefore, it is tempting to speculate that the use of antioxidants could have protective effects in glaucomatous optic neuropathy. Copyright (C) 2012 S. Karger AG, Base

Evolutionary relationships among barley and <i>Arabidopsis</i> core circadian clock and clock-associated genes

The circadian clock regulates a multitude of plant developmental and metabolic processes. In crop species, it contributes significantly to plant performance and productivity and to the adaptation and geographical range over which crops can be grown. To understand the clock in barley and how it relates to the components in the Arabidopsis thaliana clock, we have performed a systematic analysis of core circadian clock and clock-associated genes in barley, Arabidopsis and another eight species including tomato, potato, a range of monocotyledonous species and the moss, Physcomitrella patens. We have identified orthologues and paralogues of Arabidopsis genes which are conserved in all species, monocot/dicot differences, species-specific differences and variation in gene copy number (e.g. gene duplications among the various species). We propose that the common ancestor of barley and Arabidopsis had two-thirds of the key clock components identified in Arabidopsis prior to the separation of the monocot/dicot groups. After this separation, multiple independent gene duplication events took place in both monocot and dicot ancestors. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00239-015-9665-0) contains supplementary material, which is available to authorized users

Complex patterns of human antisera reactivity to novel 2009 H1N1 and historical H1N1 influenza strains

Background: During the 2009 influenza pandemic, individuals over the age of 60 had the lowest incidence of infection with approximately 25% of these people having pre-existing, cross-reactive antibodies to novel 2009 H1N1 influenza isolates. It was proposed that older people had pre-existing antibodies induced by previous 1918-like virus infection(s) that cross-reacted to novel H1N1 strains. Methodology/Principal Findings: Using antisera collected from a cohort of individuals collected before the second wave of novel H1N1 infections, only a minority of individuals with 1918 influenza specific antibodies also demonstrated hemagglutination-inhibition activity against the novel H1N1 influenza. In this study, we examined human antisera collected from individuals that ranged between the ages of 1 month and 90 years to determine the profile of seropositive influenza immunity to viruses representing H1N1 antigenic eras over the past 100 years. Even though HAI titers to novel 2009 H1N1 and the 1918 H1N1 influenza viruses were positively associated, the association was far from perfect, particularly for the older and younger age groups. Conclusions/Significance: Therefore, there may be a complex set of immune responses that are retained in people infected with seasonal H1N1 that can contribute to the reduced rates of H1N1 influenza infection in older populations. © 2012 Carter et al

Assessing and Selecting Sustainable and Resilient Suppliers in Agri-Food Supply Chains Using Artificial Intelligence: A Short Review

[EN] The supplier evaluation and selection process is critical to increase the sustainability and resilience of the agri-food supply chain. Therefore, in this sector, it is necessary to consider sustainability and resilience criteria in the supplier evaluation and selection process. The use of arti¿cial intelligence techniques allows managing of a lot of information and the reduction of uncertainty for decision making. The objective of this article is to analyze articles that address the selection of suppliers in agrifood supply chains that pursue to increase their sustainability and resilience by using arti¿cial intelligence techniques to analyze the techniques and criteria used and draw conclusions.Authors of this publication acknowledge the contribution of the Project 691249, RUC-APS "Enhancing and implementing Knowledge based ICT solutions within high Risk and Uncertain Conditions for Agriculture Production Systems" (www.ruc-aps.eu), funded by the European Union under their funding scheme H2020-MSCA-RISE-2015.Zavala-Alcívar, A.; Verdecho Sáez, MJ.; Alfaro Saiz, JJ. (2020). Assessing and Selecting Sustainable and Resilient Suppliers in Agri-Food Supply Chains Using Artificial Intelligence: A Short Review. IFIP Advances in Information and Communication Technology. 598:501-510. https://doi.org/10.1007/978-3-030-62412-5_41S501510598Brandenburg, M., Govindan, K., Sarkis, J., Seuring, S.: Quantitative models for sustainable supply chain management: developments and directions. Eur. J. Oper. Res. 233, 299–312 (2014)Ocampo, L.A., Abad, G.K.M., Cabusas, K.G.L., Padon, M.L.A., Sevilla, N.C.: Recent approaches to supplier selection: a review of literature within 2006–2016. Int. J. Integr. Supply Manage. 12, 22–68 (2018)Valipour, S., Safaei, A.: A resilience approach for supplier selection: using Fuzzy analytic network process and grey VIKOR techniques. J. Clean. Prod. 161, 431–451 (2017)Amindoust, A.: A resilient-sustainable based supplier selection model using a hybrid intelligent method. Comput. Ind. Eng. 126, 122–135 (2018)Zavala-Alcívar, A., Verdecho, M.-J., Alfaro-Saiz, J.-J.: A conceptual framework to manage resilience and increase sustainability in the supply chain. Sustainability 12(16), 6300 (2020)Villalobos, J.R., Soto-Silva, W.E., González-Araya, M.C., González-Ramirez, R.G.: Research directions in technology development to support real-time decisions of fresh produce logistics: A review and research agenda. Comput. Electron. Agric. 167, 105092 (2019)Ristono, A., Santoso, P.B., Tama, I.P.: A literature review of design of criteria for supplier selection. J. Ind. Eng. Manage. 11, 680–696 (2018)Torres-Ruiz, A., Ravindran, A.R.: Multiple criteria framework for the sustainability risk assessment of a supplier portfolio. J. Clean. Prod. 172, 4478–4493 (2018)Setak, M., Sharifi, S., Alimohammadian, A.: Supplier selection and order allocation models in supply chain management: a review. World Appl. Sci. J. 18, 55–72 (2012)Ravindran, A.R., Warsing, D.P.: Supplier selection models and methods. In: Supply Chain Engineering: Models and Applications. Taylor and Francis Group, Boca Raton, Florida (2013)De Boer, L., Labro, E., Morlacchi, P.: A review of methods supporting supplier selection. Eur. J. Purch. Supply Manage. 7, 75–89 (2011)De Felice, F., Deldoost, M.H., Faizollahi, M., Petrillo, A.: Performance measurement model for the supplier selection based on AHP. Int. J. Eng. Bus. Manag. 7, 1–13 (2015)Zimmer, K., Fröhling, M., Schultmann, F.: Sustainable supplier management – a review of models supporting sustainable supplier selection, monitoring and development. Int. J. Prod. Res. 54, 1412–1442 (2016)Christopher, M., Peck, H.: Building the resilient supply chain. Int. J. Logist. Manag. 15, 1–14 (2014)Ali, A., Mahfouz, A., Arisha, A.: Analysing supply chain resilience: integrating the constructs in a concept mapping framework via a systematic literature review. Supply Chain Manage. 22, 16–39 (2017)Verdecho, M., Alarcón-Valero, F., Pérez-Perales, D., et al.: A methodology to select suppliers to increase sustainability within supply chains. Cent. Eur. J. Oper. Res. (2020). https://doi.org/10.1007/s10100-019-00668-3Rabelo, L., Bhide, S., Gutierrez, E.: Artificial Intelligence: Advances in Research and Applications. Nova Science Publishers, Inc., Department of Industrial Engineering and Management Systems, University of Central Florida, Orlando, FL, United States (2017)Denyer, D., Tranfield, D.: Producing a systematic review. In: The Sage Handbook of Organizational Research Methods. SAGE Publications Ltd., pp. 671–689 (2019)Chen, Y.-J.: Structured methodology for supplier selection and evaluation in a supply chain. Inf. Sci. (Ny) 181, 1651–1670 (2011)Hamdi, F., Ghorbel, A., Masmoudi, F., Dupont, L.: Optimization of a supply portfolio in the context of supply chain risk management: literature review. J. Intell. Manuf. 29(4), 763–788 (2015). https://doi.org/10.1007/s10845-015-1128-3Kumar, V., Srinivasan, S., Das, S.: Optimal solution for supplier selection based on SMART fuzzy case base approach. In: 2014 Joint 7th International Conference on Soft Computing and Intelligent Systems. SCIS 2014 and 15th International Symposium on Advanced Intelligent Systems. ISIS 2014, Institute of Electrical and Electronics Engineers Inc., Department of Computer Science, IISJ Yokohama, Tokai Chiba, Japan, pp. 386–391 (2014)Jahani, A., Murad, M.A.A., bin Sulaiman, M.N., Selamat, M.H.: An agent-based supplier selection framework: Fuzzy case-based reasoning perspective. Strateg. Outsourcing 8, 180–205 (2015)Wang, Q.: Hybrid knowledge-based flexible supplier selection. In: 8th International Conference on Management of e-Commerce and e-Government. ICMeCG 2014. Institute of Electrical and Electronics Engineers Inc., Department of Information Management, Shanghai Finance University, Shanghai, China, pp. 235–239 (2014)Bai, C., Sarkis, J.: Green supplier development: analytical evaluation using rough set theory. J. Clean. Prod. 18, 1200–1210 (2010)Bai, C., Sarkis, J.: Integrating sustainability into supplier selection with grey system and rough set methodologies. Int. J. Prod. Econ. 124, 252–264 (2010)Guo, F., Lu, Q.: Partner selection optimization model of agricultural enterprises in supply chain. Adv. J. Food Sci. Technol. 5, 1285–1291 (2013)Azadnia, A.H., Saman, M.Z.M., Wong, K.Y.: Sustainable supplier selection and order lot-sizing: an integrated multi-objective decision-making process. Int. J. Prod. Res. 53, 383–408 (2015)Miranda-Ackerman, M.A., Azzaro-Pantel, C., Aguilar-Lasserre, A.A.: A green supply chain network design framework for the processed food industry: application to the orange juice agrofood cluster. Comput. Ind. Eng. 109, 369–389 (2017)Hajikhani, A., Khalilzadeh, M., Sadjadi, S.J.: A fuzzy multi-objective multi-product supplier selection and order-allocation problem in supply chain under coverage and price considerations: an urban agricultural case study. Sci. Iran. 25, 431–449 (2018)Zhang, H., Cui, Y.: A model combining a Bayesian network with a modified genetic algorithm for green supplier selection. Simulation 95, 1165–1183 (2019)Yadav, S., Garg, D., Luthra, S.: Selection of third-party logistics services for internet of things-based agriculture supply chain management. Int. J. Logist. Syst. Manage. 35, 204–230 (2020)Yazdani, M., Wang, Z.X., Chan, F.T.S.: A decision support model based on the combined structure of DEMATEL, QFD and fuzzy values. Soft. Comput. 24(16), 12449–12468 (2020). https://doi.org/10.1007/s00500-020-04685-2Zhang, H., Feng, H., Cui, Y., Wang, Y.: A fuzzy Bayesian network model for quality control in O2O e-commerce. Int. J. Comput. Commun. Control 15(1), (2020). article number 1003. https://doi.org/10.15837/ijccc.2020.1.3783Amiri, S.A.H.S., Zahedi, A., Kazemi, M., Soroor, J., Hajiaghaei-Keshteli, M.: Determination of the optimal sales level of perishable goods in a two-echelon supply chain network. Comput. Ind. Eng. 139, 106156 (2020)Roy, S., et al.: A framework for sustainable supplier selection with transportation criteria. Int. J. Sustain. Eng. 13(2), 77–92 (2020)Parkouhi, S.V., Ghadikolaei, A.S., Lajimi, H.F.: Resilient supplier selection and segmentation in grey environment. J. Clean. Prod. 207, 1123–1137 (2019)Camarinha-Matos, L.M., Afsarmanesh, H., Galeano, N., Molina, A.: Collaborative networked organizations – concepts and practice in manufacturing enterprises. Comput. Ind. Eng. 57, 46–60 (2009)Lezoche, M., Panetto, H., Kacprzyk, J., Hernandez, J., Díaz, M.A.: Agri-food 4.0: a survey of the supply chains and technologies for the future agriculture. Comput. Ind. 117, 103187 (2020)Alikhani, R., Torabi, S., Altay, N.: Strategic supplier selection under sustainability and risk criteria. Int. J. Prod. Econ. 208, 69–82 (2019

CD83 Modulates B Cell Function In Vitro: Increased IL-10 and Reduced Ig Secretion by CD83Tg B Cells

The murine transmembrane glycoprotein CD83 is an important regulator for both thymic T cell maturation and peripheral T cell responses. Recently, we reported that CD83 also has a function on B cells: Ubiquitous transgenic (Tg) expression of CD83 interfered with the immunoglobulin (Ig) response to infectious agents and to T cell dependent as well as T cell independent model antigen immunization. Here we compare the function of CD83Tg B cells that overexpress CD83 and CD83 mutant (CD83mu) B cells that display a drastically reduced CD83 expression. Correlating with CD83 expression, the basic as well as the lipopolysaccharide (LPS) induced expression of the activation markers CD86 and MHC-II are significantly increased in CD83Tg B cells and reciprocally decreased in CD83mu B cells. Wild-type B cells rapidly upregulate CD83 within three hours post BCR or TLR engagement by de novo protein synthesis. The forced premature overexpression of CD83 on the CD83Tg B cells results in reduced calcium signaling, reduced Ig secretion and a reciprocally increased IL-10 production upon in vitro activation. This altered phenotype is mediated by CD83 expressed on the B cells themselves, since it is observed in the absence of accessory cells. In line with this finding, purified CD83mu B cells displayed a reduced IL-10 production and slightly increased Ig secretion upon LPS stimulation in vitro. Taken together, our data strongly suggest that CD83 is expressed by B cells upon activation and contributes to the regulation of B cell function

Trastuzumab Mediated T-Cell Response against HER-2/Neu Overexpressing Esophageal Adenocarcinoma Depends on Intact Antigen Processing Machinery

BACKGROUND: Esophageal adenocarcinoma (EAC) is a highly aggressive disease with poor prognosis, which frequently exhibits HER-2 gene amplification. Trastuzumab, the humanized antibody against HER-2, has potent growth inhibitory effects on HER-2 overexpressing cancers. One effect of trastuzumab is that it causes HER-2 receptor internalization and degradation, enhancing presentation of HER-2 epitopes on MHC-Class I molecules. This enhances the ability of HER-2 specific cytotoxic T lymphocytes (CTLs) to recognize and kill cancer cells. Novel strategies targeting the HER-2 receptor either directly by trastuzumab and/or indirectly by inducing a CTL response against HER-2 epitopes with, for instance, DC immunotherapy and consequently combining these strategies might prove to be very effective. METHODOLOGY/PRINCIPAL FINDINGS: In this study we report that trastuzumab has potent growth inhibitory effects on two HER-2 overexpressing EAC cell lines OE33 and OE19. However, we found that trastuzumab and HER-2 specific CTLs act synergistically in inducing tumor lysis in OE33 but not in OE19. We discovered that in OE19 this deficient response is due to a down-regulation of the Transporter Associated with Antigen Processing-2 (TAP-2). TAP-2 is an important member of the Antigen Processing Machinery (APM), and is one of the essential elements for loading antigens on MHC class I molecules. Importantly, we demonstrated that by inducing re-expression of TAP-2 in OE19 with INF-γ treatment or by incubating the cells with INF-γ producing CTLs, the specific anti HER-2 CTL tumor lysis response and synergistic effect with trastuzumab can be restored. CONCLUSION: An inefficient response of HER-2 overexpressing EAC to trastuzumab and/or DC immunotherapy can be due to a down-regulated TAP-2 expression and thus a deficient APM. Future studies combining trastuzumab with IFN-γ and/or immune-therapies inducing potent anti HER-2 CTL responses could lead to an effective combinatorial strategy for successful treatment of HER-2 overexpressing but APM defective cancer

Human NK Cells Differ More in Their KIR2DL1-Dependent Thresholds for HLA-Cw6-Mediated Inhibition than in Their Maximal Killing Capacity

In this study we have addressed the question of how activation and inhibition of human NK cells is regulated by the expression level of MHC class I protein on target cells. Using target cell transfectants sorted to stably express different levels of the MHC class I protein HLA-Cw6, we show that induction of degranulation and that of IFN-γ secretion are not correlated. In contrast, the inhibition of these two processes by MHC class-I occurs at the same level of class I MHC protein. Primary human NK cell clones were found to differ in the amount of target MHC class I protein required for their inhibition, rather than in their maximum killing capacity. Importantly, we show that KIR2DL1 expression determines the thresholds (in terms of MHC I protein levels) required for NK cell inhibition, while the expression of other receptors such as LIR1 is less important. Furthermore, using mathematical models to explore the dynamics of target cell killing, we found that the observed delay in target cell killing is exhibited by a model in which NK cells require some activation or priming, such that each cell can lyse a target cell only after being activated by a first encounter with the same or a different target cell, but not by models which lack this feature