Airborne measurements of reflectivity and albedo of urban and rural surfaces of Megacities

Spectral reflectivity and albedo are obtained from airborne measurements of spectral irradiance and radiance during two field campaigns in Leipzig, Germany and Zhongshan, China. The data measured above urban and rural areas have been investigated with respect to the heterogeneity and anisotropy of the surface. Furthermore the spectral albedo and reflectivity measured above the same surface but at different flight altitudes have been analyzed. These data is used to estimate the impact of multiple scattering processes by aerosol particles and gas molecules

Design of a new hairpin DNAzyme: The activity controlled by TMPyP4

A new 10-23 hairpin DNAzyme with a G-quadruplex structure stem was designed. The formation and stability of the G-quadruplex structure as the DNAzyme stem in the absence or the presence of TMPyP4 were investigated by UV-visible spectroscopy, circular dichroism (CD) and differential scanning calorimetry (DSC) methods, respectively. The results showed that the stability of this DNAzyme can be enhanced greatly due to the interaction between TMPyP4 and the DNAzyme. The relationship between structural stability and activity of the DNAzyme was studied by in vitro catalytic reaction. The activity of this DNAzyme was regulated by the stability of DNAzyme when TMPyP4 was intercalated into Gquadruplex structure stem. The catalytic activity of the 10-23 hairpin DNAzyme decreased and even inactivated due to the enhanced stability of G-quadruplex structure by TMPyP4 molecules. This DNAzyme is controllable to cleavage substrate and has some potential significance in gene therapy.Key words: 10-23 DNAzyme, hairpin DNAzyme, G-quadruplex structure, TMPyP4

The Candida albicans transcription factor Cas5 couples stress responses, drug resistance and cell cycle regulation

We thank Cowen lab members for helpful discussions. We also thank David Rogers (University of Tennessee) for sharing microarray analysis of the CAS5 homozygous mutant, and Li Ang (University of Macau) for assistance in optimizing the ChIP-Seq experiments. J.L.X. is supported by a Canadian Institutes of Health Research Doctoral award and M.D.L. is supported by a Sir Henry Wellcome Postdoctoral Fellowship (Wellcome Trust 096072). B.T.G. holds an Ontario Graduate Scholarship. C.B. and B.J.A. are supported by the Canadian Institutes of Health Research Foundation Grants (FDN-143264 and -143265). D.J.K. is supported by a National Institute of Allergy and Infectious Diseases grant (1R01AI098450) and J.D.L.C.D. is supported by the University of Rochester School of Dentistry and Medicine PREP program (R25 GM064133). A.S. is supported by the Creighton University and the Nebraska Department of Health and Human Services (LB506-2017-55). K.H.W. is supported by the Science and Technology Development Fund of Macau S.A.R. (FDCT; 085/2014/A2). L.E.C. is supported by the Canadian Institutes of Health Research Operating Grants (MOP-86452 and MOP-119520), the Natural Sciences and Engineering Council (NSERC) of Canada Discovery Grants (06261 and 462167), and an NSERC E.W.R. Steacie Memorial Fellowship (477598).Peer reviewedPublisher PD

Multi-angular polarimetric remote sensing to pinpoint global aerosol absorption and direct radiative forcing

Quantitative estimations of atmospheric aerosol absorption are rather uncertain due to the lack of reliable information about the global distribution. Because the information about aerosol properties is commonly provided by single-viewing photometric satellite sensors that are not sensitive to aerosol absorption. Consequently, the uncertainty in aerosol radiative forcing remains one of the largest in the Assessment Reports of the Intergovernmental Panel on Climate Change (IPCC AR5 and AR6). Here, we use multi-angular polarimeters (MAP) to provide constraints on emission of absorbing aerosol species and estimate global aerosol absorption optical depth (AAOD) and its climate effect. Our estimate of modern-era mid-visible AAOD is 0.0070 that is higher than IPCC by a factor of 1.3-1.8. The black carbon instantaneous direct radiative forcing (BC DRF) is +0.33&thinsp;W/m2 [+0.17, +0.54]. The MAP constraint narrows the 95% confidence interval of BC DRF by a factor of 2 and boosts confidence in its spatial distribution. &nbsp;</p

Pleiotropic effects of the wheat domestication gene Q on yield and grain morphology

Transformation from q to Q during wheat domestication functioned outside the boundary of threshability to increase yield, grains m−2, grain weight and roundness, but to reduce grains per spike/spikelet. Mutation of the Q gene, well-known affecting wheat spike structure, represents a key domestication step in the formation of today’s free-threshing, economically important wheats. In a previous study, multiple yield components and spike characteristics were associated with the Q gene interval in the bread wheat ‘Forno’ × European spelt ‘Oberkulmer’ recombinant inbred line population. Here, we reported that this interval was also associated with grain yield, grains m−2, grain morphology, and spike dry weight at anthesis. To clarify the roles of Q in agronomic trait performance, a functional marker for the Q gene was developed. Analysis of allelic effects showed that the bread wheat Q allele conferred free-threshing habit, soft glumes, and short and compact spikes compared with q. In addition, the Q allele contributed to higher grain yield, more grains m−2, and higher thousand grain weight, whereas q contributed to more grains per spike/spikelet likely resulting from increased preanthesis spike growth. For grain morphology, the Q allele was associated with reduced ratio of grain length to height, indicating a rounder grain. These results are supported by analysis of four Q mutant lines in the Chinese Spring background. Therefore, the transition from q to Q during wheat domestication had profound effects on grain yield and grain shape evolution as well, being a consequence of pleiotropy

Airborne measurements of reflectivity and albedo of urban and rural surfaces of Megacities

Spectral reflectivity and albedo are obtained from airborne measurements of spectral irradiance and radiance during two field campaigns in Leipzig, Germany and Zhongshan, China. The data measured above urban and rural areas have been investigated with respect to the heterogeneity and anisotropy of the surface. Furthermore the spectral albedo and reflectivity measured above the same surface but at different flight altitudes have been analyzed. These data is used to estimate the impact of multiple scattering processes by aerosol particles and gas molecules