491 research outputs found
Satellite Radar Altimetry for Inland Hydrologic Studies
This research is conducted under the supervision of Dr. C.K. Shum, Professor of
Geodetic Science, School of Earth Sciences, The Ohio State University. The research
results documented in this report resulted in a PhD Dissertation. NASA and CNES
provided the TOPEX/POSEIDON (Geophysical Data Record and Sensor Data Record,
GDR and SDR) data products; LEGOS, USDA/NASA/GSFC provided high-level radar
altimetry data products; ANA Brazil, and Environment Canada provided the stage gauge
data used for this research. This research is supported by grants from NSF’s Hydrology
Program (EAR-0440007) and NGA’s NURI Program (HM1582-07-1-2024), and the
study was conducted with the objective to contribute to the Climate, Water, and Carbon
Program at The Ohio State University.Satellite radar altimetry, which is originally designed to measure global ocean surface
height, has been applied to inland surface water hydrologic studies. We have developed a
water-detection algorithm based on statistical analysis of decadal TOPEX/POSEIDON height
measurement time series, used the backscatter coefficient to classify the inland surface
properties, and the 10-Hz (corresponding to an along track spatial resolution of 700m) radar
waveform-retracked TOPEX data, to be able to observe small (<300Km2) inland bodies of
water for hydrologic studies. We applied the algorithm to the selected study regions in
Manitoba and northwestern (SW) Ontario, Canada, Amazon River Basin, and southwestern
Taiwan. Finally we studied the application of TOPEX altimetry to the 1997 Red River flood
monitoring. For the study regions in western Manitoba, the correlation coefficient between
stage and TOPEX altimetry data in the large Lakes reaches 0.98 using the 10-Hz retracked
data, thus verifying the validity and accuracy of the satellite measurement. The importance of
the waveform retracking for the inland water applications is validated by the improvement of
the correlation coefficients from 0.34 to 0.87 before and after retracking. We detected the
bodies of water, which are otherwise missed by using the original 1-Hz data from the
Geophysical Data Records, and illustrated that a higher spatial resolution could be achieved
using the individual 10-Hz retracked data. In the Amazon River Basin, the capability of the
water-detection algorithm is compared with the use of a high water level mask generated by
SAR and other data with a spatial resolution of 100m. It is shown that the algorithm could
detect the bodies of water, which are missed by the mask primarily because that the
frequency of water fluctuation is more than twice a year at some locations. The bodies of
water detected only by the algorithm are confirmed using the detailed local hydrological
maps in 3 tested regions. The retrieved water height over the small (<300Km2) body of water
was compared with the nearby stage measurement and showed good seasonal agreement. In
the southwest Taiwan, the monthly variation of 10-Hz AGC from 1992 to 2002 were
examined, it is found that the high AGC values could be used to indicate inundated area. We
detected the annual and semi-annual variations from the 10-Hz AGC and 10-Hz retracked
water height time series, which are attributable to two rainy seasons per year in the study
region. For the study of the 1997 Red River flood, we compared the geographic distribution
of 0 σ0 before, during and after the 1997 flood and found the high 0 σ0 values (>35dB)
indicate the inundated regions. In addition, the comparison of the geographically distributed
0 σ0 during Winter, Spring, Summer and Autumn of 1997 showed that the low 0 σ values
(<10dB) indicate snow coverage. The retrieved water height measurements in the flooded
regions are compared with the nearby USGS stage measurements and showed good
agreements. The comparison of 10-Hz individual retracked measurements with the 1-Hz nonretracked
height measurements confirmed the importance of the retracked data (with higher
spatial variations) in the flood monitoring. Using 0 σ0 and the retrieved water height
measurements, we detected the 1997 flooded regions include the Red River Basin of the
North in North Dakota and in western Minnesota, the upper Mississippi River Basin in
Minnesota, the Missouri River Basin in southern North Dakota and in South Dakota. The
observed flood extents from TOPEX agree well with and complement the USGS stage gauge
records
Satellite Radar Altimetry for Inland Hydrologic Studies
This research is conducted under the supervision of Dr. C.K. Shum, Professor of
Geodetic Science, School of Earth Sciences, The Ohio State University. The research
results documented in this report resulted in a PhD Dissertation. NASA and CNES
provided the TOPEX/POSEIDON (Geophysical Data Record and Sensor Data Record,
GDR and SDR) data products; LEGOS, USDA/NASA/GSFC provided high-level radar
altimetry data products; ANA Brazil, and Environment Canada provided the stage gauge
data used for this research. This research is supported by grants from NSF’s Hydrology
Program (EAR-0440007) and NGA’s NURI Program (HM1582-07-1-2024), and the
study was conducted with the objective to contribute to the Climate, Water, and Carbon
Program at The Ohio State University.Satellite radar altimetry, which is originally designed to measure global ocean surface
height, has been applied to inland surface water hydrologic studies. We have developed a
water-detection algorithm based on statistical analysis of decadal TOPEX/POSEIDON height
measurement time series, used the backscatter coefficient to classify the inland surface
properties, and the 10-Hz (corresponding to an along track spatial resolution of 700m) radar
waveform-retracked TOPEX data, to be able to observe small (<300Km2) inland bodies of
water for hydrologic studies. We applied the algorithm to the selected study regions in
Manitoba and northwestern (SW) Ontario, Canada, Amazon River Basin, and southwestern
Taiwan. Finally we studied the application of TOPEX altimetry to the 1997 Red River flood
monitoring. For the study regions in western Manitoba, the correlation coefficient between
stage and TOPEX altimetry data in the large Lakes reaches 0.98 using the 10-Hz retracked
data, thus verifying the validity and accuracy of the satellite measurement. The importance of
the waveform retracking for the inland water applications is validated by the improvement of
the correlation coefficients from 0.34 to 0.87 before and after retracking. We detected the
bodies of water, which are otherwise missed by using the original 1-Hz data from the
Geophysical Data Records, and illustrated that a higher spatial resolution could be achieved
using the individual 10-Hz retracked data. In the Amazon River Basin, the capability of the
water-detection algorithm is compared with the use of a high water level mask generated by
SAR and other data with a spatial resolution of 100m. It is shown that the algorithm could
detect the bodies of water, which are missed by the mask primarily because that the
frequency of water fluctuation is more than twice a year at some locations. The bodies of
water detected only by the algorithm are confirmed using the detailed local hydrological
maps in 3 tested regions. The retrieved water height over the small (<300Km2) body of water
was compared with the nearby stage measurement and showed good seasonal agreement. In
the southwest Taiwan, the monthly variation of 10-Hz AGC from 1992 to 2002 were
examined, it is found that the high AGC values could be used to indicate inundated area. We
detected the annual and semi-annual variations from the 10-Hz AGC and 10-Hz retracked
water height time series, which are attributable to two rainy seasons per year in the study
region. For the study of the 1997 Red River flood, we compared the geographic distribution
of 0 σ0 before, during and after the 1997 flood and found the high 0 σ0 values (>35dB)
indicate the inundated regions. In addition, the comparison of the geographically distributed
0 σ0 during Winter, Spring, Summer and Autumn of 1997 showed that the low 0 σ values
(<10dB) indicate snow coverage. The retrieved water height measurements in the flooded
regions are compared with the nearby USGS stage measurements and showed good
agreements. The comparison of 10-Hz individual retracked measurements with the 1-Hz nonretracked
height measurements confirmed the importance of the retracked data (with higher
spatial variations) in the flood monitoring. Using 0 σ0 and the retrieved water height
measurements, we detected the 1997 flooded regions include the Red River Basin of the
North in North Dakota and in western Minnesota, the upper Mississippi River Basin in
Minnesota, the Missouri River Basin in southern North Dakota and in South Dakota. The
observed flood extents from TOPEX agree well with and complement the USGS stage gauge
records
Effects of 1-methylcyclopropene on superficial scald and related metabolism in ‘Wujiuxiang’ pears during cold storage
‘Wujiuxiang’ (Pyrus bretschneideri R. × Pyrus communis L.) pears often suffer from superficial scald after long-term cold storage. In this study, harvested ‘Wujiuxiang’ pear fruits were fumigated with 1-MCP at concentrations of 0.5 μL/L and 1.0 μL/L and subsequently stored at low temperature (0 °C). The superficial scald index; flesh firmness; total soluble solids (TSS) content; respiration and ethylene production rates; relative membrane permeability; concentrations of α-farnesene, conjugated trienols (CTols), and hydrogen peroxide (H2O2); and activities of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and lipoxygenase (LOX) of the peel were investigated. The results showed that compared with control, 1-MCP reduced the index of superficial scald; maintained a higher firmness and a lower TSS content; inhibited the accumulation of H2O2, α-farnesene and conjugated trienols and the increase in cell membrane permeability; and maintained a higher activity of APX, SOD and CAT and a lower activity of LOX. These findings indicate that 1-MCP regulates the activities of H2O2-scavenging enzymes to inhibit the accumulation of H2O2 and thereby reduces cell membrane damage and inhibits the accumulation of conjugated trienols. Thus, 1-MCP could decrease the incidence of superficial scald in ‘Wujiuxiang’ pears
The effects of three different grinding methods in DNA extraction of cowpea (Vigna unguiculata L. Walp)
Rapid DNA extraction is a prerequisite for molecular studies. Generally, plant tissue is ground in liquid nitrogen to isolate DNA; but, liquid nitrogen is dangerous and volatile. Besides, liquid nitrogen is not always available in many developing countries. To investigate if high quality DNA could be obtained for downstream PCR analysis without liquid nitrogen, the cowpea DNA was extracted by Hexadecyl trimethyl ammonium bromide cetyl trimethylammonium bromide (CTAB) method and sodium dodecyl sulphate (SDS) method, respectively, each with three different grinding methods, including ground in liquid nitrogen, in preheated mortar and in non-preheated mortar. The DNA was compared according to their yield, purity, integrity and functionality. The results showed that high quality DNA could be obtained by three grinding methods both in CTAB method and SDS method. Without liquid nitrogen, grinding plant tissue in preheated or non-preheated mortar with extraction buffer to extract DNA is feasible.Keywords: Cowpea (Vigna unguiculata), grinding method, liquid nitrogen, DNA extractionAfrican Journal of Biotechnology Vol. 12(16), pp. 1946-195
Study on Motivations and Cultivation of Cultural Industry Cluster in Jilin Province, China
Abstract Cultural industry is honored as one of the industries in the 21 st Century that has the greatest development prospect. With its innovativeness, competitiveness and value added, it has become the leading edge and high end among the emerging industries in the current world, and it has also become one of the characteristic industries that have the greatest development potential in transferring the economic growth mode, promoting swift and coordinated development of regional economy in the old industrial bases. Cultural industry is playing a more and more important role in development of regional economy. Cluster is an important feature of cultural industry and cluster development is an inexorable trend in development of cultural industry. Cultural industry in Jilin Province in recent years has been rapidly developed. Nevertheless, there still exist quite a lot of problems and drawbacks. Thus, Jilin Province should find out its own characteristic factors according to its local cultural characteristics and start out from improving cultural industry policy, constructing a cultural industry cluster pattern that has comparative advantages and local features, cultivating leading key cultural enterprises and balancing cultural industrial development of all cities so as to diminish gap between different areas and improving talent security system, do a good job in taking the path of cultural industry cluster development and form cluster scale effect
The influence of foveal lexical processing load on parafoveal preview and saccadic targeting during Chinese reading
Whether increased foveal load causes a reduction of parafoveal processing remains equivocal. The present study examined foveal load effects on parafoveal processing in natural Chinese reading. Parafoveal preview of a single-character parafoveal target word was manipulated by using the boundary paradigm (Rayner, 1975; pseudocharacter or identity previews) under high foveal load (low-frequency pretarget word) compared with low foveal load (high-frequency pretarget word) conditions. Despite an effective manipulation of foveal processing load, we obtained no evidence of any modulatory influence on parafoveal processing in first-pass reading times. However, our results clearly showed that saccadic targeting, in relation to forward saccade length from the pretarget word and in relation to target word skipping, was influenced by foveal load and this influence occurred independent of parafoveal preview. Given the optimal experimental conditions, these results provide very strong evidence that preview benefit is not modulated by foveal lexical load during Chinese reading. (PsycINFO Database Record (c) 2019 APA, all rights reserved
A Biodegradable Polyethylenimine-Based Vector Modified by Trifunctional Peptide R18 for Enhancing Gene Transfection Efficiency In Vivo
Lack of capacity to cross the nucleus membrane seems to be one of the main
reasons for the lower transfection efficiency of gene vectors observed in vivo
study than in vitro. To solve this problem, a new non-viral gene vector was
designed. First, a degradable polyethylenimine (PEI) derivate was synthesized
by crosslinking low-molecular-weight (LMW) PEI with N-octyl-N-quaternary
chitosan (OTMCS), and then adopting a designed trifunctional peptide (RGDC-
TAT-NLS) with good tumor targeting, cell uptake and nucleus transport
capabilities to modify OTMCS-PEI. The new gene vector was termed as OTMCS-
PEI-R18 and characterized in terms of its chemical structure and biophysical
parameters. Gene transfection efficiency and nucleus transport mechanism of
this vector were also evaluated. The polymer showed controlled degradation and
remarkable buffer capabilities with the particle size around 100–300 nm and
the zeta potential ranged from 5 mV to 40 mV. Agraose gel electrophoresis
showed that OTMCS-PEI-R18 could effectively condensed plasmid DNA at a ratio
of 1.0. Besides, the polymer was stable in the presence of sodium heparin and
could resist digestion by DNase I at a concentration of 63U DNase I/DNA.
OTMCS-PEI-R18 also showed much lower cytotoxicity and better transfection
rates compared to polymers OTMCS-PEI-R13, OTMCS-PEI and PEI 25 KDa in vitro
and in vivo. Furthermore, OTMCS-PEI-R18/DNA complexes could accumulate in the
nucleus well soon and not rely on mitosis absolutely due to the newly
incorporated ligand peptide NLS with the specific nuclear delivery pathway
indicating that the gene delivery system OTMCS-PEI-R18 could reinforce gene
transfection efficiency in vivo
Parafoveal Previews and Lexical Frequency in Natural Reading: Evidence from Eye Movements and Fixation-Related Potentials
Participants’ eye movements and EEG signal were recorded as they read sentences displayed according to the gaze-contingent boundary paradigm. Two target words in each sentence were manipulated for lexical frequency (high vs. low frequency) and parafoveal preview of each target word (identical vs. string of random letters vs. string of Xs). Eye movement data revealed visual parafoveal-on-foveal effects (POF), as well as foveal visual and orthographic preview effects and word frequency effects. Fixation-related potentials (FRPs) showed visual and orthographic PoF effects as well as foveal visual and orthographic preview effects. Our results replicated the early preview positivity effect (Dimigen et al., 2012) in the X-string preview condition, and revealed different neural correlates associated with a preview comprised of a string of random letters relative to a string of Xs. The former effects seem likely to reflect difficulty associated with the integration of parafoveal and foveal information, as well as feature overlap, while the latter reflect inhibition, and potentially disruption, to processing underlying reading. Interestingly, and consistent with Kretzschmar, Schlesewsky and Staub (2015), no frequency effect was reflected in the FRP measures. The findings provide insight into the neural correlates of parafoveal processing and written word recognition in reading and demonstrate the value of utilising ecologically valid paradigms to study well established phenomena that occur as text is read naturally
Efficient detoxication of hydroxylamine and nitrite through heterotrophic nitrification and aerobic denitrification by Acinetobacter johnsonii EN-J1
The co-existence of hydroxylamine (NH2OH) and nitrite (NO2–-N) can aggravate the difficulty of wastewater treatment. The roles of hydroxylamine (NH2OH) and nitrite (NO2–-N) in accelerating the elimination of multiple nitrogen sources by a novel isolated strain of Acinetobacter johnsonii EN-J1 were investigated in this study. The results demonstrated that strain EN-J1 could eliminate 100.00% of NH2OH (22.73 mg/L) and 90.09% of NO2–-N (55.32 mg/L), with maximum consumption rates of 1.22 and 6.75 mg/L/h, respectively. Prominently, the toxic substances NH2OH and NO2–-N could both facilitate nitrogen removal rates. Compared with the control treatment, the elimination rates of nitrate (NO3–-N) and NO2–-N were enhanced by 3.44 and 2.36 mg/L/h after supplementation with 10.00 mg/L NH2OH, and those of ammonium (NH4+-N) and NO3–-N were improved by 0.65 and 1.00 mg/L/h after the addition of 50.00 mg/L NO2–-N. Furthermore, the nitrogen balance results indicated that over 55.00% of the initial total nitrogen was transformed into gaseous nitrogen by heterotrophic nitrification and aerobic denitrification (HN-AD). Ammonia monooxygenase (AMO), hydroxylamine oxidoreductase (HAO), nitrate reductase (NR), and nitrite reductase (NIR), which are essential for HN-AD, were detected at levels of 0.54, 0.15, 0.14, and 0.01 U/mg protein, respectively. All findings confirmed that strain EN-J1 could efficiently execute HN-AD, detoxify NH2OH and NO2–-N, and ultimately promote nitrogen removal rates
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