Microfluidic Blood Cell Sorting: Now and Beyond

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/106971/1/smll201302907.pd

Paternal chromosome elimination of inducer triggers induction of double haploids in Brassica napus

A synthetic octoploid rapeseed, Y3380, induces maternal doubled haploids when used as a pollen donor to pollinate plant. However, the mechanism underlying doubled haploid formation remains elusive. We speculated that double haploid induction occurs as the inducer line’s chromosomes pass to the maternal egg cell, and the zygote is formed through fertilization. In the process of zygotic mitosis, the paternal chromosome is specifically eliminated. Part of the paternal gene might have infiltrated the maternal genome through homologous exchange during the elimination process. Then, the zygote haploid genome doubles (early haploid doubling, EH phenomenon), and the doubled zygote continues to develop into a complete embryo, finally forming doubled haploid offspring. To test our hypothesis, in the current study, the octoploid Y3380 line was back bred with the 4122-cp4-EPSPS exogenous gene used as a marker into hexaploid Y3380-cp4-EPSPS as paternal material to pollinate three different maternal materials. The fertilization process of crossing between the inducer line and the maternal parent was observed 48 h after pollination, and the fertilization rate reached 97.92% and 98.72%. After 12 d of pollination, the presence of cp4-EPSPS in the embryo was detected by in situ PCR, and at 13–23 d after pollination, the probability of F1 embryos containing cp4-EPSPS gene was up to 97.27%, but then declined gradually to 0% at 23–33 d. At the same time, the expression of cp4-EPSPS was observed by immunofluorescence in the 3rd to 29th day embryo. As the embryos developed, cp4-EPSPS marker genes were constantly lost, accompanied by embryonic death. After 30 d, the presence of cp4-EPSPS was not detected in surviving embryos. Meanwhile, SNP detection of induced offspring confirmed the existence of double haploids, further indicating that the induction process was caused by the loss of specificity of the paternal chromosome. The tetraploid-induced offspring showed infiltration of the induced line gene loci, with heterozygosity and homozygosity. Results indicated that the induced line chromosomes were eliminated during embryonic development, and the maternal haploid chromosomes were synchronously doubled in the embryo. These findings support our hypothesis and lay a theoretical foundation for further localization or cloning of functional genes involved in double haploid induction in rapeseed

Effects of preparation process on microstructure and properties of porous Ti6Al4V

The forced sweat cooling of porous media is an effective way to solve the problem of thermal protection of the leading edge of hypersonic vehicles. The pore structure and performance of porous media have a significant impact on its cooling effect and reliability. Therefore，it is very important to prepare porous materials that meet the requirements of forced sweat cooling. Herein，Ti6Al4V pre-alloyed powders were used as raw materials，and porous Ti6Al4V samples with different open porosity were prepared by compression molding combined with high-temperature sintering. The effect of the sintering temperature and holding time on the microstructures，phase compositions and mechanical properties of the samples were investigated. The results show that increasing the sintering temperature and prolonging the holding time will reduce the open porosity of the material. When the open porosity is high，the pores in the material are connected and the seepage rate is high，while the sample strength is low. When the open porosity is low，large pores in the sample are reduced and the seepage rate decreases，but the strength becomes higher. The porous Ti6Al4V sample with an open porosity of 21.8% shows the best comprehensive performance. When the porous Ti6Al4V sample is used as active thermal protection material，it can withstand flame ablation with an average heat flux of 2.5 MW/m2

A Time-Delay-Bounded Data Scheduling Algorithm for Delay Reduction in Distributed Networked Control Systems

As a key feature of networked control systems (NCSs), the time delays induced by communication medium sharing and data exchange over the system components could largely degrade the NCS performances or may even cause system instability, and thus, it is of critical importance to reduce time delays within NCSs. This paper studies the time-delay reduction problem in distributed NCSs and presents a dual-way data scheduling mechanism for time-delay reductions in delay-bounded NCSs with time-varying delays. We assess the time delays and their influences on the NCSs first with various delay factors being considered and then describe a one-way scheduling mechanism for network-delay reductions in NCSs. Based upon such a method, a dual-way scheduling algorithm is finally proposed for distributed NCSs with different types of transmitted data packets. Experiments are conducted on a remote teaching platform to verify the effectiveness of the proposed dual-way scheduling mechanism. Results demonstrate that, with the stability time-delay bound considered within the scheduling process, the proposed mechanism is effective for NCS time-delay reductions while addressing the stability, control accuracy, and settling time issues efficiently. Such a proposed mechanism could also be implemented together with some other existing control algorithms for time-delay reductions in NCSs. Our work could provide both useful theoretical guidance and application references for stable tracking control of delay-bounded NCSs

Rapid Creation of Interspecific Hybrid Progeny to Broaden Genetic Distance through Double Haploid (DH) Inducer in Brassica napus

Interspecific hybridization of rapeseed is an important way to innovate breeding resources. This research used Brassica napus and Brassica rapa for artificial synthesis interspecific hybridization of F1. The F1 self-fruiting rate was particularly low. By comparing the fertilization rate and seed setting rate of nine crosses and selfing combinations of interspecific hybrid progeny F1 and control B. napus, the results proved that the genetic stability of egg cells was greater than that of sperm cells, so the F1 could get seed by artificial pollination with other normal pollen. Based on these results, interspecific maternal inbred offspring (induced F1) from egg cells was obtained by emasculation and pollination with the pollen of DH inducer Y3380. It was found through morphological analysis, flow cytometry identification, and meiotic observation of induced F1, the plants had most normal fertile tetraploid and the meiosis was normal. The FISH results showed that the induced F1 were B. napus (2n = 4x = 38, AACC), 20 A and 19 C chromosomes. The results of SNP chip detection and genetic cluster analysis found that the genetic variation between interspecies could be preserved or broadened in the induced F1. The use of DH inducer created special breeding resources for interspecific hybridization and distant hybridization of rapeseed while shortening time, improving efficiency, and providing a new insight into innovate breeding resources

Highly Active Cathode Achieved by Constructing Surface Proton Acid Sites through Electronic Regulation of Heteroatoms

For proton-conducting solid oxide fuel cells (PCFCs), accelerating the kinetics of the proton involved oxygen reduction reaction (P-ORR) occurring primarily on the surface of cathodes is of key importance. To this end, developing simple, efficient, and economical strategies to optimize the gas–solid interface is crucial but full of challenges. Herein, the heteroatom boron (B) is first introduced to modify the PCFC cathode surface (Pr4Ni3O10+δ, PN) by mechanical mixing method (0.5B-PN). Combined with in situ/ex situ characterizations and DFT calculation, it is found that the CO2 resistance, surface hydration ability, and surface electrocatalytic activity toward P-ORR are significantly improved by B decoration. Importantly, the B element is found to raise the surface Brønsted acid (−OH) concentration yet depress the surface Lewis acidity, both of which are conducive to P-ORR reaction. At 600 °C, the maximum power density of the cell using 0.5B-PN as the cathode improved by 149.5% compared with that using the PN cathode. This work opens up a new avenue for developing novel PCFC cathodes via nonmetallic regulation of surface

Genome-Wide Duplication of Allotetraploid Brassica napus Produces Novel Characteristics and Extensive Ploidy Variation in Self-Pollinated Progeny

Whole genome duplications (WGDs) have played a major role in angiosperm species evolution. Polyploid plants have undergone multiple cycles of ancient WGD events during their evolutionary history. However, little attention has been paid to the additional WGD of the existing allopolyploids. In this study, we explored the influences of additional WGD on the allopolyploid Brassica napus. Compared to tetraploid B. napus, octoploid B. napus (AAAACCCC, 2n = 8x =76) showed significant differences in phenotype, reproductive ability and the ploidy of self-pollinated progeny. Genome duplication also altered a key reproductive organ feature in B. napus, that is, increased the number of pollen apertures. Unlike autopolyploids produced from the diploid Brassica species, the octoploid B. napus produced from allotetraploid B. napus had a relatively stable meiotic process, high pollen viability and moderate fertility under self-pollination conditions, indicating that sub-genomic interactions may be important for the successful establishment of higher-order polyploids. Doubling the genome of B. napus provided us with an opportunity to gain insight into the flexibility of the Brassica genomes. The genome size of self-pollinated progeny of octoploid B. napus varied greatly, and was accompanied by extensive genomic instability, such as aneuploidy, mixed-ploidy and mitotic abnormality. The octoploid B. napus could go through any of genome reduction, equilibrium or expansion in the short-term, thus providing a novel karyotype library for the Brassica genus. Our results reveal the short-term evolutionary consequences of recurrent polyploidization events, and help to deepen our understanding of polyploid plant evolution