819 research outputs found
Diazotroph community succession during the VAHINE mesocosm experiment (New Caledonia lagoon)
The VAHINE mesocosm experiment, conducted in the low-nutrient low-chlorophyll waters of the Noumea lagoon (coastal New Caledonia) was designed to trace the incorporation of nitrogen (N) fixed by diazotrophs into the food web, using large volume (50 m(3)) mesocosms. This experiment provided a unique opportunity to study the succession of different N-2-fixing microorganisms (diazotrophs) and calculate in situ net growth and mortality rates in response to fertilization with dissolved inorganic phosphate (DIP) over a 23-day period, using quantitative polymerase chain reaction (qPCR) assays targeting widely distributed marine diazotroph lineages. Inside the mesocosms, the most abundant diazotroph was the heterocyst-forming Richelia associated with Rhizosolenia (Het-1) in the first half of the experiment, while unicellular cyanobacterial Group C (UCYN-C) became abundant during the second half of the experiment. Decreasing DIP concentrations following the fertilization event and increasing temperatures were significantly correlated with increasing abundances of UCYN-C. Maximum net growth rates for UCYN-C were calculated to range between 1.23 +/- 0.07 and 2.16 +/- 0.07 d(-1) in the mesocosms, which are among the highest growth rates reported for diazotrophs. Outside the mesocosms in the New Caledonia lagoon, UCYN-C abundances remained low, despite increasing temperatures, suggesting that the microbial community response to the DIP fertilization created conditions favorable for UCYN-C growth inside the mesocosms. Diazotroph community composition analysis using PCR targeting a component of the nitrogenase gene (nifH) verified that diazotrophs targeted in qPCR assays were collectively among the major lineages in the lagoon and mesocosm samples, with the exception of Crocosphaera-like phylotypes, where sequence types not typically seen in the oligotrophic ocean grew in the mesocosms. Maximum net growth and mortality rates for nine diazotroph phylotypes throughout the 23-day experiment were variable between mesocosms, and repeated fluctuations between periods of net growth and mortality were commonly observed. The field population of diazotrophs in the New Caledonian lagoon waters appeared to be dominated by Het-1 over the course of the study period. However, results from both qPCR and PCR analysis indicated a diverse field population of diazotrophs was present in the lagoon at the time of sampling. Two ecotypes of the Braarudosphaera bigelowii symbiont unicellular group A (UCYN-A) were present simultaneously in the lagoon, with the recently described B. bigelowii/UCYN-A2 association present at higher abundances than the B. bigelowii/UCYN-A1 association
Rates of Dinitrogen Fixation and the Abundance of Diazotrophs in North American Coastal Waters Between Cape Hatteras and Georges Bank
We coupled dinitrogen (N2) fixation rate estimates with molecular biological methods to determine the activity and abundance of diazotrophs in coastal waters along the temperate North American Mid-Atlantic continental shelf during multiple seasons and cruises. Volumetric rates of N2 fixation were as high as 49.8 nmol N L(sup -1) d(sup -1) and areal rates as high as 837.9 micromol N m(sup -2) d(sup -1) in our study area. Our results suggest that N2 fixation occurs at high rates in coastal shelf waters that were previously thought to be unimportant sites of N2 fixation and so were excluded from calculations of pelagic marine N2 fixation. Unicellular N2-fixing group A cyanobacteria were the most abundant diazotrophs in the Atlantic coastal waters and their abundance was comparable to, or higher than, that measured in oceanic regimes where they were discovered. High rates of N2 fixation and the high abundance of diazotrophs along the North American Mid-Atlantic continental shelf highlight the need to revise marine N budgets to include coastal N2 fixation. Integrating areal rates of N2 fixation over the continental shelf area between Cape Hatteras and Nova Scotia, the estimated N2 fixation in this temperate shelf system is about 0.02 Tmol N yr(sup -1), the amount previously calculated for the entire North Atlantic continental shelf. Additional studies should provide spatially, temporally, and seasonally resolved rate estimates from coastal systems to better constrain N inputs via N2 fixation from the neritic zone
Simultaneous administration of adjuvant donor bone marrow in pancreas transplant recipients
Objective: The effect of donor bone marrow was evaluated for its potentially favorable effect in the authors' simultaneous pancreas/kidney transplant program. Methods: From July 1994 to January 1999, 177 pancreas transplants were performed, 151 of which were simultaneous pancreas/kidney transplants. All patients received tacrolimus, mycophenolate mofetil, and steroids for immunosuppression (azathioprine was used in the first year of the program). Fifty-three simultaneous pancreas/kidney transplant recipients received perioperative unmodified donor bone marrow, 3 to 6 x 108 cells/kg. Results: Overall actuarial survival rates at 1 and 3 years were 98% and 95% (patient), 95% and 87% (kidney), and 86% and 80% (pancreas), respectively. In the adjuvant bone marrow group, 1- and 3-year survival rates were 96% and 91% (patient), 95% and 87% (kidney), and 83% and 83% (pancreas), respectively. For 98 recipients who did not receive bone marrow, survival rates at 1 and 3 years were 100% and 98% (patient), 96% and 86% (kidney), and 87% and 79% (pancreas), respectively. No pancreas allografts were lost after 3 months in bone marrow recipients, and seven in the non-bone marrow recipients were lost to rejection at 0.7, 6.7, 8.8, 14.6, 24.1, 24.3, and 25.5 months. Twenty-two percent of bone marrow patients were steroid-free at 1 year, 45% at 2 years, and 67% at 3 years. Nineteen percent of the non-bone marrow recipients were steroid-free at 1 year, 38% at 2 years, and 45% (p = 0.02) at 3 years. The mean acute cellular rejection rate was 0.94 ± 1.1 in the bone marrow group and 1.57 ± 1.3 (p = 0.003) in the non-bone marrow group (includes borderline rejection and multiple rejections). The level of donor cell chimerism in the peripheral blood of bone marrow patients was at least two logs higher than in controls. Conclusion: In this series, which represents the largest experience with adjuvant bone marrow infusion in pancreas recipients, there was a higher steroid withdrawal rate (p = 0.02), fewer rejection episodes, and no pancreas graft loss after 3 months in bone marrow recipients compared with contemporaneous controls. All pancreas allografts lost to chronic rejection (n = 6) were in the non-bone marrow group. Donor bone marrow administered around the time of surgery may have a protective effect in pancreas transplantation
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Influence of the Amazon River plume on distributions of free-living and symbiotic cyanobacteria in the western tropical north Atlantic Ocean
The vertical and horizontal distributions of seven diazotrophic populations in the western tropical north Atlantic (WTNA) Ocean were examined using a nifH DNA quantitative polymerase chain reaction (QPCR) approach. The nifH phylotype abundances were highest near the surface and decreased with depth, with the exception of the cyanobacterial symbiont Calothrix, which was not detected at any station. Richelia associated with the diatoms Rhizosolenia clevei and Hemiaulus hauckii were distributed within the freshwater lens of the Amazon plume. Abundances of H. hauckii-Richelia nifH genes dominated all depths in 6 of 10 vertical profiles and 10 of 20 surface samples. In addition, estimates of Richelia associated with H. hauckii increased northwest (8- 12°N, 56-54°W) from the river mouth, where significantly ( p 10⁵ copies L⁻¹) were found in mesohaline waters (31-34.9). nifH copy abundance for surface populations of the H. hauckii-Richelia symbioses were positively correlated (r² = 0.59) with salinity. Three unicellular cyanobacterial groups and Trichodesmium had similar horizontal distributions, where the highest nifH copy estimates were at stations with salinity ≥35 and northeast (6-10°N 50°W) of the freshwater lens. The abundance of Trichodesmium spp. and unicellular Group B nifH gene copies co-varied (r² = 0.60). The QPCR study showed the dominance of H. hauckii-Richelia symbioses in the Amazon plume waters, implying that these associations had an ecological advantage over the other diazotrophs. Outside of the plume nutrients were below detection, abundances of freeliving unicellular cyanobacterial phylotypes, including a novel group designated Group C, were abundant (>10⁵ copies L⁻¹) and comparable to the abundances of Trichodesmium spp. Thus, there appeared to be a cascade of diazotrophic communities along gradients of salinity and nutrients in the WTNA
Nitrogen fixation and transfer in open ocean diatom–cyanobacterial symbioses
Many diatoms that inhabit low-nutrient waters of the open ocean live in close association with cyanobacteria. Some of these associations are believed to be mutualistic, where N2-fixing cyanobacterial symbionts provide N for the diatoms. Rates of N2 fixation by symbiotic cyanobacteria and the N transfer to their diatom partners were measured using a high-resolution nanometer scale secondary ion mass spectrometry approach in natural populations. Cell-specific rates of N2 fixation (1.15–71.5 fmol N per cell h−1) were similar amongst the symbioses and rapid transfer (within 30 min) of fixed N was also measured. Similar growth rates for the diatoms and their symbionts were determined and the symbiotic growth rates were higher than those estimated for free-living cells. The N2 fixation rates estimated for Richelia and Calothrix symbionts were 171–420 times higher when the cells were symbiotic compared with the rates estimated for the cells living freely. When combined, the latter two results suggest that the diatom partners influence the growth and metabolism of their cyanobacterial symbionts. We estimated that Richelia fix 81–744% more N than needed for their own growth and up to 97.3% of the fixed N is transferred to the diatom partners. This study provides new information on the mechanisms controlling N input into the open ocean by symbiotic microorganisms, which are widespread and important for oceanic primary production. Further, this is the first demonstration of N transfer from an N2 fixer to a unicellular partner. These symbioses are important models for molecular regulation and nutrient exchange in symbiotic systems
Neutron skin of Pb from Coherent Pion Photoproduction
Information on the size and shape of the neutron skin on Pb has been
extracted from coherent pion photoproduction cross sections measured using the
Crystal Ball together with the Glasgow tagger at the MAMI electron beam
facility. On exploitation of an interpolated fit of a theoretical model to the
measured cross sections the half-height radius and diffuseness of the neutron
distribution are found to be 6.70 fm and 0.55 fm respectively, corresponding to a neutron
skin thickness =0.15 fm.
The results give the first successful extraction of a neutron skin with an
electromagnetic probe and indicate the skin of Pb has a halo character.
The measurement provides valuable new constraints on both the structure of
nuclei and the equation of state for neutron-rich matter.Comment: 4 figures 5 pages. Version submitted to journal. Includes additional
studies of systematic effects in the extracted diffuseness, which led to a
small increase in the quoted systematic error. These additional studies are
discussed in the revised manuscript. Also includes minor editorial
improvements to the tex
Differential distributions of Synechococcus subgroups across the California current system
Synechococcus is an abundant marine cyanobacterial genus composed of different populations that vary physiologically. Synechococcus narB gene sequences (encoding for nitrate reductase in cyanobacteria) obtained previously from isolates and the environment (e.g., North Pacific Gyre Station ALOHA, Hawaii or Monterey Bay, CA, USA) were used to develop quantitative PCR (qPCR) assays. These qPCR assays were used to quantify populations from specific narB phylogenetic clades across the California Current System (CCS), a region composed of dynamic zones between a coastal-upwelling zone and the oligotrophic Pacific Ocean. Targeted populations (narB subgroups) had different biogeographic patterns across the CCS, which appear to be driven by environmental conditions. Subgroups CC1, DC1, and DC2 were abundant in coastal-upwelling to coastal-transition zone waters with relatively high to intermediate ammonium, nitrate, and chl. a concentrations. Subgroups AC1 and FC1 were most abundant in coastal-transition zone waters with intermediate nutrient concentrations. EO1 and GO1 were most abundant at different depths of oligotrophic open-ocean waters (either in the upper mixed layer or just below). EO1, AC1, and FC1 distributions differed from other narB subgroups and likely possess unique ecologies enabling them to be most abundant in waters between coastal and open-ocean waters. Different CCS zones possessed distinct Synechococcus communities. Core California current water possessed low numbers of narB subgroups relative to counted Synechococcus cells, and coastal-transition waters contained high abundances of Synechococcus cells and total number of narB subgroups. The presented biogeographic data provides insight on the distributions and ecologies of Synechococcus present in an eastern boundary current system. © 2011 Paerl, Johnson, Welsh, Worden, Chavez and Zehr
Nutrients limitation of primary productivity in the Southeast Pacific (BIOSOPE cruise)
Revue sans Comité de lectureInternational audienceIron is an essential nutrient involved in a variety of biological processes in the ocean, including photosynthesis, respiration and nitrogen fixation. Atmospheric deposition of aerosols is recognized as the main source of iron for the surface ocean. In high nutrient, low chlorophyll areas, it is now clearly established that iron limits phytoplankton productivity but its biogeochemical role in low nutrient, low chlorophyll environments has been poorly studied. We investigated this question in the unexplored southeast Pacific, arguably the most oligotrophic area of the global ocean. Situated far from any continental aerosol source, the atmospheric iron flux to this province is amongst the lowest of the world ocean. Here we report that, despite low dissolved iron concentrations (~0.1 nmol l-1) measured across the whole gyre (3 stations situated in the center, the western and the eastern edge), photosynthesis and primary productivity are only limited by iron availability at the border of the gyre, but not in the center. The seasonal stability of the gyre has apparently allowed for the development of populations acclimated to these extreme oligotrophic conditions. Moreover, despite clear evidence of nitrogen limitation in the central gyre, we were unable to measure nitrogen fixation in our experiments, even after iron and/or phosphate additions, and cyanobacterial nifH gene abundances were extremely low compared to the North Pacific Gyre. The South Pacific gyre is therefore unique with respect to the physiological status of its phytoplankton populations
Measurement of the beam-helicity asymmetry in the photoproduction of -pairs off protons and off neutrons
Beam-helicity asymmetries have been measured at the MAMI accelerator in Mainz
for the photoproduction of mixed-charge pion pairs in the reactions
off free protons and
and
off quasi-free nucleons bound
in the deuteron for incident photon energies up to 1.4 GeV. Circularly
polarized photons were produced from bremsstrahlung of longitudinally polarized
electrons and tagged with the Glasgow-Mainz magnetic spectrometer. The charged
pions, recoil protons, recoil neutrons, and decay photons from mesons
were detected in the 4 electromagnetic calorimeter composed of the Crystal
Ball and TAPS detectors. Using a complete kinematic reconstruction of the final
state, excellent agreement was found between the results for free and
quasi-free protons, suggesting that the quasi-free neutron results are also a
close approximation of the free-neutron asymmetries. A comparison of the
results to the predictions of the Two-Pion-MAID reaction model shows that the
reaction mechanisms are still not well understood, in particular at low
incident photon energies in the second nucleon-resonance region.Comment: accepted for publication in Eur. phys. J.
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