Electrokinetic methods for preparative electrophoresis on a chip

This thesis describes research on preparative capillary electrophoresis on a chip. Capillary electrophoresis on a chip has one important drawback: the amount of an analyte obtained from a single run is very limited. Consequently, post-separation processing of the separated sample is challenging. Therefore methods for efficient, post-separation sample manipulation and handling are required. Such techniques should provide a way for pooling of separated identical components in order to provide sufficient amount of a sample for further off-chip, lab-scale processing. Alternatively, a method for continuous electrophoretic sample fractionation can be employed.\ud \ud In this project, the aim was the development of microfluidic electrokinetic-only strategies for microchip preparative CE. Particularly, the techniques for single fraction manipulation in complex channel networks were researched and methods that allow for fully automated control of such procedures were proposed. These techniques were later applied to develop a micropreparative CE chip device that was able to separate complex samples and pool identical fractions with no user interaction.\ud \ud Furthermore, an approach for continuously-operating zone electrophoresis was introduced and investigated. This new method, called continuous-flow zone electrophoresis (CFZE) separates analytes solely by electrophoresis and electro-osmosis. Unlike free-flow electrophoresis devices, no mechanical pumping is required. A preparative mode of CFZE, named synchronized, continuous-flow zone electrophoresis (SCFZE) was also investigated. This method allows for rapid and highly selective sample fractionation

Application of medical simulation in the education of medical students in the area of gynecology and obstetrics

The education of new generations of doctors faces major challenges. The education system should ensure access to modernand effective educational techniques. Medical simulation is a method that is developing very dynamically. Currently, everymedical university in Poland has access to the facilities of a Medical Simulation Centre. Many types of simulations can beused. The variety of techniques is considerable. Starting from simple trainers, through advanced patient simulators to hybridsimulation or virtual reality. Thanks to their use, it is possible to teach basic medical procedures in a safe way, withoutcompromising the patient’s intimacy. An additional advantage is the possibility to train in an interdisciplinary team. Theaim of this work was to present the possibility of using medical simulation as a method of effective and interesting teachingof medical students in the field of gynaecology and obstetrics. The authors described different techniques and levels ofsimulation sophistication. The basic tasks of the teacher were also described. The paper may be an interesting complementto the knowledge of education for each physician involved in the work with students

Application of medical simulation in the education of medical students in the area of gynecology and obstetrics

The education of new generations of doctors faces major challenges. The education system should ensure access to modern and effective educational techniques. Medical simulation is a method that is developing very dynamically. Currently, every medical university in Poland has access to the facilities of a Medical Simulation Centre. Many types of simulations can be used. The variety of techniques is considerable. Starting from simple trainers, through advanced patient simulators to hybrid simulation or virtual reality. Thanks to their use, it is possible to teach basic medical procedures in a safe way, without compromising the patient’s intimacy. An additional advantage is the possibility to train in an interdisciplinary team. The aim of this work was to present the possibility of using medical simulation as a method of effective and interesting teaching of medical students in the field of gynaecology and obstetrics. The authors described different techniques and levels of simulation sophistication. The basic tasks of the teacher were also described. The paper may be an interesting complement to the knowledge of education for each physician involved in the work with students

The effectiveness of ‘practice while watching’ technique for the first aid training of the chemical industry employees

INTRODUCTION: Industrial accidents constitute about 20% of all causes of accidents. They are often sudden, unexpected and may lead to tragic consequences, which however can be partially reduced if first aid and emergency treatment are given as soon as possible. The most important part of a chain of survival is the bystanders’ response. In practice, it is highly dependent on the ability of co-workers to perform basic first-aid tasks. The aim of this paper was to present the effectiveness of ‘practice while watching’ training method for the first aid training of the chemical industry employees. The authors focused on the most important elements of the training, which have an impact on the acquisition of knowledge and skills of first aid.  MATERIAL AND METHODS: The training involved 69 employees of the chemical industry. The test sheets were used for the assessment, where each of the action steps was assigned points 0 for incorrect and 1 for a properly performed activity.  RESULTS: Each step was completed by at least 76% of workers. When providing both adult and infant CPR, the main difficulty was calling for help. Younger employees obtained better results than older colleagues.  CONCLUSIONS: The study demonstrated the high effectiveness of the ‘practice while watching’ method. In addition, workers’ age turned out to be a significant variable which affected their performance.

Use of video laryngoscopes by inexperienced personnel in difficult intubations

The current gold standard in securing airway patency remains to be endotracheal intubation. It is the only method, which allows for nearly 100% protection of the bronchial tree from aspiration of gastric contents as well as providing the most ideal circumstances for control of ventilation parameters. Endotracheal intu- bation, although in many aspects superior to other methods of securing airways, can only be performed by skilled and experienced personnel in ideal conditions. An example of such conditions are in an operating room in the preoperative period when an anesthesiologist is able to proficiently perform the task with all of the tools and equipment needed at hand. However, in many situations, especially in emergencies, such ideal conditions are difficult or impossible to achieve. One of the many reasons behind this is often the lack of experienced personnel at the scene of an emergency. Another significant difficulty arises from trauma patients who must maintain an immobilized cervical spine, as well as those patients who are undergoing active cardiopulmonary resuscitation when providing high quality chest compressions is the highest priority. Therefore, it seems reasonable to look for the methods which on one hand will secure an airway with a tube inserted directly into the larynx, and on the other hand will make the procedure more accessible to less expe- rienced personnel by maintaining the proper patient safety throughout the whole procedure. A noteworthy method, which achieves this goal, is the use of the video laryngoscopes for endotracheal intubation. The participation in a short introductory training, regarding the use of the device itself, is sufficient to allow for the efficient intubation. The parameters which can be used to compare these different intubation methods include the ease of use, the rate of effectiveness of the first intubation trial as well as the total time needed for the procedure. The authors of this article attempt to compare classic laryngoscopes to video-assisted laryngoscopes. 

Paramedic students need more training in left ventricular assist device — a pilot simulation study

INTRODUCTION: Mechanical Circulation Systems are a promising therapy for patients with end-stage of heart failure. Left ventricular assist device (LVAD) enforces using of concomitant anticoagulant therapy. This may lead to severe complications. LVAD patients are more and more frequent users of the emergency department. There are several differences in cardiovascular function in these patients, as well as on examination. Its interpretation may be challenging and result in potentially fatal conclusions. The aim of this research was to assess the skills of paramedic students in assessing patients with LVAD MATERIAL AND METHODS: The study was designed as a simulation study. The aim of this scenario was to provide a full primary survey of an unconscious, spontaneously breathing person with an LVAD pump implanted. Ten groups of paramedic students from Polish medical universities took part in this study. RESULTS: Four teams started chest compressions unnecessarily. Of them, only one had contacted LVAD local coordinator and discontinued after short instructions. Four teams completed the driveline and device check and six checked only the line without moving the controller. No major errors were noted in the field of airway assessment and management as well as assessment of consciousness, breathing, and circulation. CONCLUSIONS: More attention should be paid to educating paramedic students in LVAD therapy. Educators should focus mainly on differences in cardiovascular function and pay attention to complete perfusion assessment. Medical simulation seems to be a good tool for assessing difficult clinical cases rarely encountered in practice

An Optical Sensor Network for Vegetation Phenology Monitoring and Satellite Data Calibration

We present a network of sites across Fennoscandia for optical sampling of vegetation properties relevant for phenology monitoring and satellite data calibration. The network currently consists of five sites, distributed along an N-S gradient through Sweden and Finland. Two sites are located in coniferous forests, one in a deciduous forest, and two on peatland. The instrumentation consists of dual-beam sensors measuring incoming and reflected red, green, NIR, and PAR fluxes at 10-min intervals, year-round. The sensors are mounted on separate masts or in flux towers in order to capture radiation reflected from within the flux footprint of current eddy covariance measurements. Our computations and model simulations demonstrate the validity of using off-nadir sampling, and we show the results from the first year of measurement. NDVI is computed and compared to that of the MODIS instrument on-board Aqua and Terra satellite platforms. PAR fluxes are partitioned into reflected and absorbed components for the ground and canopy. The measurements demonstrate that the instrumentation provides detailed information about the vegetation phenology and variations in reflectance due to snow cover variations and vegetation development. Valuable information about PAR absorption of ground and canopy is obtained that may be linked to vegetation productivity

Discutindo a educação ambiental no cotidiano escolar: desenvolvimento de projetos na escola formação inicial e continuada de professores

A presente pesquisa buscou discutir como a Educação Ambiental (EA) vem sendo trabalhada, no Ensino Fundamental e como os docentes desta escola compreendem e vem inserindo a EA no cotidiano escolar., em uma escola estadual do município de Tangará da Serra/MT, Brasil. Para tanto, realizou-se entrevistas com os professores que fazem parte de um projeto interdisciplinar de EA na escola pesquisada. Verificou-se que o projeto da escola não vem conseguindo alcançar os objetivos propostos por: desconhecimento do mesmo, pelos professores; formação deficiente dos professores, não entendimento da EA como processo de ensino-aprendizagem, falta de recursos didáticos, planejamento inadequado das atividades. A partir dessa constatação, procurou-se debater a impossibilidade de tratar do tema fora do trabalho interdisciplinar, bem como, e principalmente, a importância de um estudo mais aprofundado de EA, vinculando teoria e prática, tanto na formação docente, como em projetos escolares, a fim de fugir do tradicional vínculo “EA e ecologia, lixo e horta”.Facultad de Humanidades y Ciencias de la Educació