211 research outputs found
Feature-Based Change Detection Reveals Inconsistent Individual Differences in Visual Working Memory Capacity
Visual working memory (VWM) is a key cognitive system that enables people to hold visual information in mind after a stimulus has been removed and compare past and present to detect changes that have occurred. VWM is severely capacity limited to around 3â4 items, although there are robust individual differences in this limit. Importantly, these individual differences are evident in neural measures of VWM capacity. Here, we capitalized on recent work showing that capacity is lower for more complex stimulus dimension. In particular, we asked whether individual differences in capacity remain consistent if capacity is shifted by a more demanding task, and, further, whether the correspondence between behavioral and neural measures holds across a shift in VWM capacity. Participants completed a change detection (CD) task with simple colors and complex shapes in an fMRI experiment. As expected, capacity was significantly lower for the shape dimension. Moreover, there were robust individual differences in behavioral estimates of VWM capacity across dimensions. Similarly, participants with a stronger BOLD response for color also showed a strong neural response for shape within the lateral occipital cortex, intraparietal sulcus (IPS), and superior IPS. Although there were robust individual differences in the behavioral and neural measures, we found little evidence of systematic brain-behavior correlations across feature dimensions. This suggests that behavioral and neural measures of capacity provide different views onto the processes that underlie VWM and CD. Recent theoretical approaches that attempt to bridge between behavioral and neural measures are well positioned to address these findings in future work
Structure of the Helicase Domain of DNA Polymerase Theta Reveals a Possible Role in the Microhomology-Mediated End-Joining Pathway
DNA polymerase theta (PolΞ) has been identified as a crucial alternative non-homologous end-joining factor in mammalian cells. PolΞ is upregulated in a range of cancer cell types defective in homologous recombination, and knockdown has been shown to inhibit cell survival in a subset of these, making it an attractive target for cancer treatment. We present crystal structures of the helicase domain of human PolΞ in the presence and absence of bound nucleotides, and a characterization of its DNA-binding and DNA-stimulated ATPase activities. Comparisons with related helicases from the Hel308 family identify several unique features. PolΞ exists as a tetramer both in the crystals and in solution. We propose a model for DNA binding to the PolΞ helicase domain in the context of the PolΞ tetramer, which suggests a role for the helicase domain in strand annealing of DNA templates for subsequent processing by the polymerase domain
Expansion of a novel endogenous retrovirus throughout the pericentromeres of modern humans
Abstract
Background
Approximately 8% of the human genome consists of sequences of retroviral origin, a result of ancestral infections of the germ line over millions of years of evolution. The most recent of these infections is attributed to members of the human endogenous retrovirus type-K (HERV-K) (HML-2) family. We recently reported that a previously undetected, large group of HERV-K (HML-2) proviruses, which are descendants of the ancestral K111 infection, are spread throughout human centromeres.
Results
Studying the genomes of certain cell lines and the DNA of healthy individuals that seemingly lack K111, we discover new HERV-K (HML-2) members hidden in pericentromeres of several human chromosomes. All are related through a common ancestor, termed K222, which is a virus that infected the germ line approximately 25 million years ago. K222 exists as a single copy in the genomes of baboons and high order primates, but not New World monkeys, suggesting that progenitor K222 infected the primate germ line after the split between New and Old World monkeys. K222 exists in modern humans at multiple loci spread across the pericentromeres of nine chromosomes, indicating it was amplified during the evolution of modern humans.
Conclusions
Copying of K222 may have occurred through recombination of the pericentromeres of different chromosomes during human evolution. Evidence of recombination between K111 and K222 suggests that these retroviral sequences have been templates for frequent cross-over events during the process of centromere recombination in humans.http://deepblue.lib.umich.edu/bitstream/2027.42/111301/1/13059_2015_Article_641.pd
Mars Aeronomy Observer: Report of the Science Working Team
The Mars Aeronomy Observer (MAO) is a candidate follow-on mission to Mars Observer (MO) in the Planetary Observer Program. The four Mariner and two Viking spacecraft sent to Mars between 1965 and 1976 have provided a wealth of information concerning Martian planetology. The Mars Observer, to be launched in 1990, will build on their results by further examining the elemental and mineralogical composition of the surface, the strength and multipolar composition of the planetary magnetic field, the gravitational field and topography, and the circulation of the lower atmosphere. The Mars Aeronomy Observer is intended to address the last major aspects of Martian environment which have yet to be investigated: the upper atmosphere, the ionsphere, and the solar wind interaction region
Models of the SL9 Impacts II. Radiative-hydrodynamic Modeling of the Plume Splashback
We model the plume "splashback" phase of the SL9 collisions with Jupiter
using the ZEUS-3D hydrodynamic code. We modified the Zeus code to include gray
radiative transport, and we present validation tests. We couple the infalling
mass and momentum fluxes of SL9 plume material (from paper I) to a jovian
atmospheric model. A strong and complex shock structure results. The modeled
shock temperatures agree well with observations, and the structure and
evolution of the modeled shocks account for the appearance of high excitation
molecular line emission after the peak of the continuum light curve. The
splashback region cools by radial expansion as well as by radiation. The
morphology of our synthetic continuum light curves agree with observations over
a broad wavelength range (0.9 to 12 microns). A feature of our ballistic plume
is a shell of mass at the highest velocities, which we term the "vanguard".
Portions of the vanguard ejected on shallow trajectories produce a lateral
shock front, whose initial expansion accounts for the "third precursors" seen
in the 2-micron light curves of the larger impacts, and for hot methane
emission at early times. Continued propagation of this lateral shock
approximately reproduces the radii, propagation speed, and centroid positions
of the large rings observed at 3-4 microns by McGregor et al. The portion of
the vanguard ejected closer to the vertical falls back with high z-component
velocities just after maximum light, producing CO emission and the "flare" seen
at 0.9 microns. The model also produces secondary maxima ("bounces") whose
amplitudes and periods are in agreement with observations.Comment: 13 pages, 9 figures (figs 3 and 4 in color), accepted for Ap.J.
latex, version including full figures at:
http://oobleck.tn.cornell.edu/jh/ast/papers/slplume2-20.ps.g
Observing the Evolution of the Universe
How did the universe evolve? The fine angular scale (l>1000) temperature and
polarization anisotropies in the CMB are a Rosetta stone for understanding the
evolution of the universe. Through detailed measurements one may address
everything from the physics of the birth of the universe to the history of star
formation and the process by which galaxies formed. One may in addition track
the evolution of the dark energy and discover the net neutrino mass.
We are at the dawn of a new era in which hundreds of square degrees of sky
can be mapped with arcminute resolution and sensitivities measured in
microKelvin. Acquiring these data requires the use of special purpose
telescopes such as the Atacama Cosmology Telescope (ACT), located in Chile, and
the South Pole Telescope (SPT). These new telescopes are outfitted with a new
generation of custom mm-wave kilo-pixel arrays. Additional instruments are in
the planning stages.Comment: Science White Paper submitted to the US Astro2010 Decadal Survey.
Full list of 177 author available at http://cmbpol.uchicago.ed
A leaky umbrella has little value: evidence clearly indicates the serotonin system is implicated in depression.
A recent âumbrellaâ review examined various biomarkers relating to the serotonin system, and concluded there was no consistent evidence implicating serotonin in the pathophysiology of depression. We present reasons for why this conclusion is overstated, including methodological weaknesses in the review process, selective reporting of data, over-simplification, and errors in the interpretation of neuropsychopharmacological findings. We use the examples of tryptophan depletion and serotonergic molecular imaging, the two research areas most relevant to the investigation of serotonin, to illustrate this
A leaky umbrella has little value:evidence clearly indicates the serotonin system is implicated in depression
A recent âumbrellaâ review examined various biomarkers relating to the serotonin system, and concluded there was no consistent evidence implicating serotonin in the pathophysiology of depression. We present reasons for why this conclusion is overstated, including methodological weaknesses in the review process, selective reporting of data, over-simplification, and errors in the interpretation of neuropsychopharmacological findings. We use the examples of tryptophan depletion and serotonergic molecular imaging, the two research areas most relevant to the investigation of serotonin, to illustrate this
Flexible modulation of agonist efficacy at the human A3 adenosine receptor by the imidazoquinoline allosteric enhancer LUF6000
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