Selenium and Health: An Update on the Situation in the Middle East and North Africa.

Selenium (Se) is an important trace element that should be present in the diet of all age groups to provide an adequate intake. Se is incorporated in 25 known selenoproteins, which mediate the biological effects of Se including, immune response regulation, maintenance of thyroid function, antioxidant defense, and anti-inflammatory actions. A balanced intake of Se is critical to achieve health benefits because depending on its status, Se has been found to play physiological roles or contribute to the pathophysiology of various diseases including, neurodegenerative diseases, diabetes, cancer, and cardiovascular disorders. Se status and intake are very important to be known for a specific population as the levels of Se are highly variable among different populations and regions. In the Middle East and North African (MENA) region, very little is known about the status of Se. Studies available show that Se status is widely variable with some countries being deficient, some over sufficient, and some sufficient. This variability was apparent even within the same country between regions. In this review, we summarized the key roles of Se in health and disease and discussed the available data on Se status and intake among countries of the MENA region

Dromedary Camel Health Care Practices Reported by Caretakers Working at a Permanent Market

Traditional health care practices and ethnoveterinary medicine are still common in camels. This study aimed at describing camel health issues reported by caretakers including their management and treatment practices. Associations between the health issues and caretakers’ background, management practices, and facilities were also investigated. Camel caretakers (n=49) at a permanent market in Qatar were interviewed and data related to the camel pens (e.g. presence of shelters, water, and feeding points) collected. During the interview, questions sought to elicit the following information; i) caretaker’s background (age, level of education, experience, and how they identify when a camel is sick), ii) caretaker’s management practices (feeding, watering, and health care practices), iii) caretaker’s description of the last health problem (camel details, recalled diagnosis/clinical signs, possible cause, treatment, and outcome). The majority of the caretakers were middle-aged (31-50 years old) and they used to identify a sick camel mainly observing its behavioural modifications (p < 0.001). Treatment for endo- and ectoparasites were usually administered by caretakers, health checks routinely performed by veterinarians, while vaccinations were not conducted in most of the pens (p < 0.001). A total of 38 health problems were described and the high environmental temperature was the most reported possible cause (43.2%; p < 0.001). Among the recalled health problems, sunstrokes were the most common (11/38; p = 0.046), followed by fever (9/38). Sunstroke was more likely to affect males (OR: 5.04; CI95%: 1.87-23.38; p = 0.039), while fever younger animals (OR: 0.68, CI95%: 0.47-1.00; p = 0.050). Sunstrokes were more likely managed by non-veterinarians (OR: 4.75; CI95%: 1.05-21.34; p = 0.042). The majority of the cases were early identified and had a full recovery, but 11/38 camels died. Interestingly, drugs were the most common treatment (73.7%, p < 0.001), but often bought and administered directly by caretakers. Duty of care, infectious disease prevention, early and appropriate treatments could be recommended to guarantee the principle of good health and consequently animal welfare. Education on how to identify a sick camel and how to manage it should be also promoted

Molecular Characterization and Study of Genetic Relationships among local Cultivars of the Moroccan fig (Ficus carica L.) using Microsatellite and ISSR Markers

Molecular characterization of Moroccan local fig (Ficus carica L.) germplasm was performed on the cultivars present in a collection of the National School of Agriculture of Meknes. A total of 22 fig samples were analysed using 7 ISSR primers and 9 loci S.S.R. A total of 54 I.S.S.R. polymorphic bands with an average of 8 per primers and 42 S.S.R. alleles with means 5 alleles per locus were revealed by these analyses. The ISSR markers allowed distinguishing 22 molecular profiles and S.S.R. loci differentiated between 21 different profiles. Pairwise Comparing, 87% of cultivars pairs were differentiated by 7 to 24 alleles and 89% by 9 to 29 ISSR bands. The statistical analysis and genetic distances have shown a wide molecular diversity in the collection, where the average observed heterozygosity was 0.42. The average similarity between cultivars is 70% using SSR markers and 71.6 for ISSR markers. The same SSR profile was obtained for Nabout1 and Nabout2 with 0 allele difference. Small differences of 1 to 6 alleles were obtained among cultivars which have the same names, which presumably corresponds to somaclonal variations obtained through intense vegetative propagation over long periods, while the differences over 7 alleles suggests the problems of homonyms

Improved Innate and Adaptive Immunostimulation by Genetically Modified HIV-1 Protein Expressing NYVAC Vectors.

Attenuated poxviruses are safe and capable of expressing foreign antigens. Poxviruses are applied in veterinary vaccination and explored as candidate vaccines for humans. However, poxviruses express multiple genes encoding proteins that interfere with components of the innate and adaptive immune response. This manuscript describes two strategies aimed to improve the immunogenicity of the highly attenuated, host-range restricted poxvirus NYVAC: deletion of the viral gene encoding type-I interferon-binding protein and development of attenuated replication-competent NYVAC. We evaluated these newly generated NYVAC mutants, encoding HIV-1 env, gag, pol and nef, for their ability to stimulate HIV-specific CD8 T-cell responses in vitro from blood mononuclear cells of HIV-infected subjects. The new vectors were evaluated and compared to the parental NYVAC vector in dendritic cells (DCs), RNA expression arrays, HIV gag expression and cross-presentation assays in vitro. Deletion of type-I interferon-binding protein enhanced expression of interferon and interferon-induced genes in DCs, and increased maturation of infected DCs. Restoration of replication competence induced activation of pathways involving antigen processing and presentation. Also, replication-competent NYVAC showed increased Gag expression in infected cells, permitting enhanced cross-presentation to HIV-specific CD8 T cells and proliferation of HIV-specific memory CD8 T-cells in vitro. The recombinant NYVAC combining both modifications induced interferon-induced genes and genes involved in antigen processing and presentation, as well as increased Gag expression. This combined replication-competent NYVAC is a promising candidate for the next generation of HIV vaccines

Théorèmes de Structure sur Certaines Algèbres m-Convexes Commutatives

Nous donnons dans ce travail une caractérisation des algèbres (semi-simples) localement-convexes complètes faiblement topologisées au sens de S. Warner, ce qui clarifie, entre autres, plusiers résultats données sur certaines classes d'algèbres à base étudiées par de nombreux auteurs ([2], [6], [7]) pour approcher le problème de E. A. Michael sur la continuité des caractères dans les algèbres de Fréchet [9]

Dromedary camel health care practices reported by caretakers working at a permanent market

Traditional health care practices and ethnoveterinary medicine are still common in camels. This study aimed at describing camel health issues reported by caretakers including their management and treatment practices. Associations between the health issues and caretakers’ background, management practices, and facilities were also investigated. Camel caretakers (n=49) at a permanent market in Qatar were interviewed and data related to the camel pens (e.g. presence of shelters, water, and feeding points) collected. During the interview, questions sought to elicit the following information; i) caretaker’s background (age, level of education, experience, and how they identify when a camel is sick), ii) caretaker’s management practices (feeding, watering, and health care practices), iii) caretaker’s description of the last health problem (camel details, recalled diagnosis/clinical signs, possible cause, treatment, and outcome). The majority of the caretakers were middle-aged (31-50 years old) and they used to identify a sick camel mainly observing its behavioural modifications (p < 0.001). Treatment for endo- and ectoparasites were usually administered by caretakers, health checks routinely performed by veterinarians, while vaccinations were not conducted in most of the pens (p < 0.001). A total of 38 health problems were described and the high environmental temperature was the most reported possible cause (43.2%; p < 0.001). Among the recalled health problems, sunstrokes were the most common (11/38; p = 0.046), followed by fever (9/38). Sunstroke was more likely to affect males (OR: 5.04; CI95%: 1.87-23.38; p = 0.039), while fever younger animals (OR: 0.68, CI95%: 0.47-1.00; p = 0.050). Sunstrokes were more likely managed by non-veterinarians (OR: 4.75; CI95%: 1.05-21.34; p = 0.042). The majority of the cases were early identified and had a full recovery, but 11/38 camels died. Interestingly, drugs were the most common treatment (73.7%, p < 0.001), but often bought and administered directly by caretakers. Duty of care, infectious disease prevention, early and appropriate treatments could be recommended to guarantee the principle of good health and consequently animal welfare. Education on how to identify a sick camel and how to manage it should be also promoted

Elucidation of Physiological, Transcriptomic and Metabolomic Salinity Response Mechanisms in Medicago sativa

Alfalfa (Medicago sativa L.) is a widely grown perennial leguminous forage crop with a number of positive attributes. However, despite its moderate ability to tolerate saline soils, which are increasing in prevalence worldwide, it suffers considerable yield declines under these growth conditions. While a general framework of the cascade of events involved in plant salinity response has been unraveled in recent years, many gaps remain in our understanding of the precise molecular mechanisms involved in this process, particularly in non-model yet economically important species such as alfalfa. Therefore, as a means of further elucidating salinity response mechanisms in this species, we carried out in-depth physiological assessments of M. sativa cv. Beaver, as well as transcriptomic and untargeted metabolomic evaluations of leaf tissues, following extended exposure to salinity (grown for 3–4 weeks under saline treatment) and control conditions. In addition to the substantial growth and photosynthetic reductions observed under salinity treatment, we identified 1233 significant differentially expressed genes between growth conditions, as well as 60 annotated differentially accumulated metabolites. Taken together, our results suggest that changes to cell membranes and walls, cuticular and/or epicuticular waxes, osmoprotectant levels, antioxidant-related metabolic pathways, and the expression of genes encoding ion transporters, protective proteins, and transcription factors are likely involved in alfalfa’s salinity response process. Although some of these alterations may contribute to alfalfa’s modest salinity resilience, it is feasible that several may be disadvantageous in this context and could therefore provide valuable targets for the further improvement of tolerance to this stress in the future.Science, Faculty ofNon UBCChemistry, Department ofReviewedFacultyResearcherGraduat