3,844 research outputs found
Trade-off between somatic and germline repair in a vertebrate supports the "expensive germ line" hypothesis
The disposable soma theory is a central tenet of the biology of aging where germline immortality comes at the cost of an aging soma [T. B. L. Kirkwood, Nature 270, 301â304 (1977); T. B. L. Kirkwood, Proc. R. Soc. Lond. B Biol. Sci. 205, 531â546 (1979); T. B. L. Kirkwood, S. N. Austad, Nature 408, 233â238 (2000)]. Limited resources and a possible trade-off between the repair and maintenance of the germ cells and growth and maintenance of the soma may explain the deterioration of the soma over time. Here we show that germline removal allows accelerated somatic healing under stress. We tested âthe expensive germ lineâ hypothesis by generating germline-free zebrafish Danio rerio and testing the effect of the presence and absence of the germ line on somatic repair under benign and stressful conditions. We exposed male fish to sublethal low-dose ionizing radiation, a genotoxic stress affecting the soma and the germ line, and tested how fast the soma recovered following partial fin ablation. We found that somatic recovery from ablation occurred substantially faster in irradiated germline-free fish than in the control germline-carrying fish where somatic recovery was stunned. The germ line did show signs of postirradiation recovery in germline-carrying fish in several traits related to offspring number and fitness. These results support the theoretical conjecture that germline maintenance is costly and directly trades off with somatic maintenance
Structure of the saxiphilin:saxitoxin (STX) complex reveals a convergent molecular recognition strategy for paralytic toxins.
Dinoflagelates and cyanobacteria produce saxitoxin (STX), a lethal bis-guanidinium neurotoxin causing paralytic shellfish poisoning. A number of metazoans have soluble STX-binding proteins that may prevent STX intoxication. However, their STX molecular recognition mechanisms remain unknown. Here, we present structures of saxiphilin (Sxph), a bullfrog high-affinity STX-binding protein, alone and bound to STX. The structures reveal a novel high-affinity STX-binding site built from a "proto-pocket" on a transferrin scaffold that also bears thyroglobulin domain protease inhibitor repeats. Comparison of Sxph and voltage-gated sodium channel STX-binding sites reveals a convergent toxin recognition strategy comprising a largely rigid binding site where acidic side chains and a cation-Ï interaction engage STX. These studies reveal molecular rules for STX recognition, outline how a toxin-binding site can be built on a naĂŻve scaffold, and open a path to developing protein sensors for environmental STX monitoring and new biologics for STX intoxication mitigation
The Impact of the Minimum Leverage Ratio on South Dakota Community Banks
https://scholar.dsu.edu/research-symposium/1020/thumbnail.jp
Bank risk in a decade of low interest rates
A low interest rate regime remains in place in the U.S. after the Financial Crisis of 2008. Banks nevertheless need to find ways to boost the economic value to shareholders. This research examine whether it is possible for banks to stay the course and pursue profitable yet riskier assets or investments regardless of the fact that regulators have put restrictions on banksâ asset portfolio formation and capital ratio. This study hypothesizes that banks still engage in highly risky yet profitable investments or services to offset low interest income even after the 2008 Financial Crisis. A panel VAR model and a dynamic GMM model incorporating two structural breaks are employed to examine bank data obtained from the FFIEC from 2003 thru 2014. This study suggests that banks, especially larger banks, still have strong incentives to undertake riskier projects with higher expected returns in order to increase their performance. This has implications for policy makers examining risks inherent to the banking system
Root exudates impact plant performance under abiotic stress
Plant root exudates serve pivotal roles in supporting plant development and interactions with the physicochemical and biological factors in the rhizosphere. Under stress conditions, root exudation is involved in enhancing plant resource-use efficiency and facilitating the crosstalk between plant and soil microbes to ameliorate stress. Although there are a large number of root exudates that remain to be characterized, recent technological advancements have allowed for the function of many exudate compounds to be elucidated. In this review, we discuss current knowledge about the key root exudates that modulate plant resource-use efficiency under various abiotic stresses including drought, aluminum toxicity, phosphorus, nitrogen, and iron deficiency. The role that key root exudates play in shaping microbial communities in the rhizosphere under stress conditions is also an important consideration addressed in this review
Assessment of Bacterial Inoculant Delivery Methods for Cereal Crops
Despite growing evidence that plant growth-promoting bacteria can be used to improve crop vigor, a comparison of the different methods of delivery to determine which is optimal has not been published. An optimal inoculation method ensures that the inoculant colonizes the host plant so that its potential for plant growth-promotion is fully evaluated. The objective of this study was to compare the efficacy of three seed coating methods, seedling priming, and soil drench for delivering three bacterial inoculants to the sorghum rhizosphere and root endosphere. The methods were compared across multiple time points under axenic conditions and colonization efficiency was determined by quantitative polymerase chain reaction (qPCR). Two seed coating methods were also assessed in the field to test the reproducibility of the greenhouse results under non-sterile conditions. In the greenhouse seed coating methods were more successful in delivering the Gram-positive inoculant (Terrabacter sp.) while better colonization from the Gram-negative bacteria (Chitinophaga pinensis and Caulobacter rhizosphaerae) was observed with seedling priming and soil drench. This suggested that Gram-positive bacteria may be more suitable for the seed coating methods possibly because of their thick peptidoglycan cell wall. We also demonstrated that prolonged seed coating for 12 h could effectively enhance the colonization of C. pinensis, an endophytic bacterium, but not the rhizosphere colonizing C. rhizosphaerae. In the field only a small amount of inoculant was detected in the rhizosphere. This comparison demonstrates the importance of using the appropriate inoculation method for testing different types of bacteria for their plant growth-promotion potential
Simple and Versatile Route to the Synthesis of Anisotropic Bimetallic Core-Shell and Monometallic Hollow Nanostructures: Ag (AgCl)-Pt Core-Shell Nanocubes and Pt Nanoboxes
We report herewith a simple and versatile route for the preparation of anisotropic Ag(AgCl)-Pt core-shell nanocubes and Pt nanoboxes. The core-shell nanocubes were first synthesized through the simultaneous reduction method and then treated with bis-(p-sulfonatophenyl)-phenylphosphine (BSPP) to remove the core materials. The changes in morphology, structure and composition during these syntheses were carefully followed. We found that, BSPP, in addition to being an effective silver oxidant, is also a good solubilizer for AgCl nanoparticles at room temperature. This allowed us to prepare pure Pt nanoboxes easily from the as-synthesized Ag (AgCl)-Pt nanocubes using a greatly simplified post-treatment for AgCl, which is the perennial impurity byproduct in the preparation of hollow nanostructures by the replacement reactions.Singapore-MIT Alliance (SMA
Regulation of menaquinone production in Desulfovibrio vulgaris Hildenborough and Desulfovibrio desulfuricans strain G20 [abstract]
Abstract only availableMenaquinones are cytoplasmic membrane components that have important roles in electron transport for energy generation in anaerobic bacteria. A simple and sensitive protocol for the purification and quantification of menaquinones from the sulfate-reducing anaerobes Desulfovibrio vulgaris Hildenborough and Desulfovibrio desulfuricans strain G20 has been established. This method was used to determine whether differences occurred in menaquinone production in cells grown by fermentation when compared with those grown by respiration. Purified menaquinone extracts from both species were analyzed using thin-layer chromatography, UV absorption spectroscopy, and high-performance liquid chromatography. It was observed that each species possesses the same two menaquinones but the ratio between the two menaquinones differed in each species. The effects of alternate growth modes on the menaquinone content of the cells is being explored. Methods to further characterize and identify the two menaquinones is currently in progress
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