A New Suaeda Record For Flora of Turkey: Suaeda Aegyptiaca (Hasselquist) Zohary (Chenopodiaceae/Amaranthaceae)

DergiPark: 903661trkjnatBu çalışmada, Şanlıurfa ili Akçakale ilçesinden Suaeda aegyptiaca (Hasselquist) Zohary türü Türkiye florası için yeni kayıt olarak verilmektedir. Tür, Suaedoideae altfamilyası, Suaeda Forssk. ex J.F. Gmel. cinsi Salsina Moq. seksiyonu altında sınıflandırılmıştır. Türün kapsamlı betimi, Türkiye’deki dağılış haritası, habitat özellikleri, morfolojik karakterleri ve fotoğrafları verilmiştir.In this study, Suaeda aegyptiaca (Hasselquist) Zohary is reported as a new record for Turkish flora from Akçakale district in Şanlıurfa province. The species is classified under section Salsina Moq. of the genus Suaeda Forssk. ex J.F. Gmel. in Suaedoideae subfamily. The comprehensive description, distribution maps in Turkey, habitat features, morphological characteristics and digital images of the species are given

Microcnemum coralloides (Chenopodiaceae-Salicornioideae): un ejemplo de las disyunciones intraespecíficas Este-Oeste en la región mediterránea

Microcnemum is a monotypic genus of Salicornioideae comprising rare, annual, hygrohalophytic herbs growing in hypersaline inland lagoons and salt pans. Microcnemum coralloides shows an East-West disjunction in the Mediterranean region: M. coralloides subsp. coralloides occurs in central and eastern Spain while M. coralloides subsp. anatolicum grows in Turkey, Syria, Armenia and Iran. We studied the phylogeny, biogeography and morphological differentiation of M. coralloides. Molecular analyses, using five western and eight eastern accessions of the species, were based on three different markers (nuclear ITS and plastid atpB-rbcL spacer and trnT/F region) analysed with Maximum Parsimony and Maximum Likelihood. Estimates of divergence times were calculated using a Likelihood Ratio Test (LRT) and the Penalized Likelihood (PL) method. The two subspecies can be clearly distinguished by their different seed testa surface. Other diagnostic characters were not found. The molecular data (ITS and ML analysis of the trnT/F region) indicate that M. coralloides subsp. coralloides originated from within M. coralloides subsp. anatolicum which implies an East Mediterranean origin and subsequent westward dispersal. Age estimates for the split of the two subspecies range from 2.8–0.5 million years ago. Considering the relatively low genetic differentiation and the low crown group age (0.7–0.1 mya) of M. coralloides subsp. coralloides in comparison to M. coralloides subsp. anatolicum we favour the hypothesis that the Iberian part of the species range was established during cold periods of the Early Pleistocene and that the range of the species was fragmented during a warmer period soon after its arrival in Iberia.Microcnemum es un género monotípico de Salicornioideae que consiste en hierbas higrohalófilas, anuales, raras, que crecen en cuencas endorréicas hipersalinas del interior y salares. Microcnemum coralloides muestran una disyunción Este-Oeste en la región mediterránea: M. coralloides subsp. coralloides aparece en el centro y el levante español, mientras que M.coralloides subsp. anatolicum crece en Turquía, Siria, Armenia e Irán. Estudiamos la filogenia, la biogeografía y la diferenciación morfológica de M. coralloides. Los análisis moleculares, empleando cinco adquisiciones occidentales y ocho orientales de la especie, se basaron en tres marcadores distintos (ITS nuclear, espaciadores plástidos atpBrbcL y región trnT/F) analizados con Máxima Parsimonia y Máxima Verosimilitud. Las estimaciones de tiempos divergentes se calcularon empleando una Prueba de Verosimilitud (LRT) y el método de Verosimilitud Penalizada. Las dos subspecies se distinguen claramente por la diferencia en la superficie de su envoltura. No se encontraron otras características de diferenciación. Los datos moleculares (ITS y análisis ML de la región trnT/F) indican que M. coralloides subsp. coralloides originó de dentro de M. coralloides subsp. anatolicum, lo cual implica un origen en el este del Mediterráneo y su posterior dispersión hacia el oeste. Las estimaciones de edad para la separación de las dos subespecies data desde hace 2,8 a 0,5 millones de años. En vista de la diferenciación genética, relativamente baja y la reducida edad del grupo terminal (0,7-0,1 millones de años) de M. coralloides subsp. coralloides en comparación con M. coralloides subsp. anatolicum, favorecemos la hipótesis de que la parte ibérica de la gama de la especie se estableció durante periodos fríos del Bajo Pleistoceno y que la gama de la especie se fragmentó durante un periodo más cálido no mucho después de su llegada a la Península

Conservation Status of Three Rare and Endemic Species From Turkey (Kalidium Wagenitzii, Muscari Adilii Verbascum Gypsicola)

DergiPark: 751851trkjnatBu çalışma ile Türkiye’nin İç Anadolu bölgesi için endemik olan K. wagenitzii (Aellen) Freitag amp; G. Kadereit, M. adilii M. B. Güner amp; H. Duman ve V. gypsicola Vural amp; Aydoğdu türlerinin popülasyon yapıları ve yayılış alanlarının belirlenmesi ve bunların sonucunda IUCN tehlike kategorilerinin tekrardan değerlendirilmesi amaçlanmıştır. Bu türlerin seçilmesinin nedeni sahip oldukları sınırlı yayılış alanları ile insan faktörü sonucu yüksek oranda yok olma riski taşımalarıdır. Çok yıllık halofitik K. wagenitzii türünün Tuz Gölü çevresinde 5 lokalitede yayılışı belirlenmiş ve toplam olgun birey sayısı 6458 olarak belirlenmiştir. Yaşam alanları toplamı 36 km2 olmasına karşın yayılış alanı 213 km2 dir. Tarım amaçlı kontrolsüz su kaynaklarının kullanılması önemli bir tehdit faktörüdür. K. wagenitzii türünün tehdit kategorisi IUCN Kırmızı Liste kriterlerine göre EN olarak değerlendirilmiştir. M. adilii marnlı toprakları tercih etmekte ve habitat tipi P. nigra ve meşe ormanlarının yıkımı sonucu oluşan steplerdir. Nallıhan-Beypazarı çevresinde 3 lokaliteden bilinmekte ve olgun birey sayısı 6144 olarak belirlenmiştir. Yaşam alanları toplamı ve yayılış alanı sırasıyla 12 km2 ve 28 km2 dir. Tür üzerindeki baskılar yol inşası ve tarım alanlarının büyümesi, ağaçlandırma çalışmaları ve fabrika kurulumu olup CR kategorisinde değerlendirilmiştir. V. gypsicola marnlı topraklarda yayılmaktadır. Beypazarı-Nallıhan çevresinden 3 lokalite ve Sivirihisar-Eskişehir’de bir lokalite yayılışı bulunmaktadır. Olgun birey sayısı 2755 olarak belirlenmiştir. Yaşam alanları toplamı 16 km2 ve yayılış alanı 269 km2 dir. Aşırı otlatma ve tarım alanlarının büyümesi ile birlikte fabrika kurulumu tür üzerindeki baskılar olup EN olarak değerlendirilmiştir.In this study, we aimed to determine the population size and distribution areas and to re-evaluate IUCN threat categories of Kalidium wagenitzii (Aellen) Freitag amp; G. Kadereit, Muscari adilii M.B. Güner amp; H. Duman and Verbascum gypsicola Vural amp; Aydoğdu, which are endemics of the Central Anatolia region of Turkey. The three species have limited distribution areas and high risk of extinction as a result of human impact. The perennial halophytic species K. wagenitzii was found in 5 localities around Salt Lake (Tuz Gölü) and a total number of 6458 mature individuals were determined. The area of occupancy of the species is 36 km2 and the extent of occurrence is 213 km2. Uncontrolled use of water resources for agricultural purposes is a serious threat factor for the species. Kalidium wagenitzii is listed in EN category according to the IUCN Red List criteria. Muscari adilii prefers marly soils and its habitat type is formed by secondary succession after the destruction of Pinus nigra and oak forests. It is known from 3 localities around Nallıhan-Beypazarı with a population size of 6144 mature individuals. The area of occupancy and the extent of occurrence of the species is 12 km2 and 28 km2, respectively. The pressures on the species are road construction and increase of farmland, afforestation and factory establishment. According to the IUCN Red List criteria, the species is listed in CR category. Verbascum gypsicola is distributed on marly soils. It is known from 3 localities around Nallıhan–Beypazarı and one locality in Sivrihisar-Eskişehir with 2755 mature individuals in total. The area of occupancy of the species is 16 km2 and the extent of occurrence is 269 km2. Overgrazing and expansion of agricultural land, together with factory establishment are the major threats for this species, which is listed as EN in IUCN Red List.

"Microcnemum coralloides" (Chenopodiaceae-Salicornioideae): an example of intraspecific East-West disjunctions in the Mediterranean region

Kadereit, G. & A.E. Yaprak. 2008. Microcnemum coralloides (Chenopodiaceae-Salicornioideae): un ejemplo de las disyunciones intraespecíficas Este-Oeste en la región mediterránea. Anales Jard. Bot. Madrid 65(2): 415-426 (en inglés). Microcnemum es un género monotípico de Salicornioideae que consiste en hierbas higrohalófilas, anuales, raras, que crecen en cuencas endorréicas hipersalinas del interior y salares. Microcnemum coralloides muestran una disyunción Este-Oeste en la región mediterránea: M. coralloides subsp. coralloides aparece en el centro y el levante español, mientras que M.coralloides subsp. anatolicum crece en Turquía, Siria, Armenia e Irán. Estudiamos la filogenia, la biogeografía y la diferenciación morfológica de M. coralloides. Los análisis moleculares, empleando cinco adquisiciones occidentales y ocho orientales de la especie, se basaron en tres marcadores distintos (ITS nuclear, espaciadores plástidos atpBrbcL y región trnT/F) analizados con Máxima Parsimonia y Máxima Verosimilitud. Las estimaciones de tiempos divergentes se calcularon empleando una Prueba de Verosimilitud (LRT) y el método de Verosimilitud Penalizada. Las dos subspecies se distinguen claramente por la diferencia en la superficie de su envoltura. No se encontraron otras características de diferenciación. Los datos moleculares (ITS y análisis ML de la región trnT/F) indican que M. coralloides subsp. coralloides originó de dentro de M. coralloides subsp. anatolicum, lo cual implica un origen en el este del Mediterráneo y su posterior dispersión hacia el oeste. Las estimaciones de edad para la separación de las dos subespecies data desde hace 2,8 a 0,5 millones de años. En vista de la diferenciación genética, relativamente baja y la reducida edad del grupo terminal (0,7-0,1 millones de años) de M. coralloides subsp. coralloides en comparación con M. coralloides subsp. anatolicum, favorecemos la hipótesis de que la parte ibérica de la gama de la especie se estableció durante periodos fríos del Bajo Pleistoceno y que la gama de la especie se fragmentó durante un periodo más cálido no mucho después de su llegada a la Península.Kadereit, G. & A.E. Yaprak. 2008. Microcnemum coralloides (Chenopodiaceae-Salicornioideae): an example of intraspecific East-West disjunctions in the Mediterranean region. Anales Jard. Bot. Madrid 65(2): 415-426. Microcnemum is a monotypic genus of Salicornioideae comprising rare, annual, hygrohalophytic herbs growing in hypersaline inland lagoons and salt pans. Microcnemum coralloides shows an East-West disjunction in the Mediterranean region: M. coralloides subsp. coralloides occurs in central and eastern Spain while M. coralloides subsp. anatolicum grows in Turkey, Syria, Armenia and Iran. We studied the phylogeny, biogeography and morphological differentiation of M. coralloides. Molecular analyses, using five western and eight eastern accessions of the species, were based on three different markers (nuclear ITS and plastid atpB-rbcL spacer and trnT/F region) analysed with Maximum Parsimony and Maximum Likelihood. Estimates of divergence times were calculated using a Likelihood Ratio Test (LRT) and the Penalized Likelihood (PL) method. The two subspecies can be clearly distinguished by their different seed testa surface. Other diagnostic characters were not found. The molecular data (ITS and ML analysis of the trnT/F region) indicate that M. coralloides subsp. coralloides originated from within M. coralloides subsp. anatolicum which implies an East Mediterranean origin and subsequent westward dispersal. Age estimates for the split of the two subspecies range from 2.8�0.5 million years ago. Considering the relatively low genetic differentiation and the low crown group age (0.7�0.1 mya) of M. coralloides subsp. coralloides in comparison to M. coralloides subsp. anatolicum we favour the hypothesis that the Iberian part of the species range was established during cold periods of the Early Pleistocene and that the range of the species was fragmented during a warmer period soon after its arrival in Iberia

Assessing water quality in the Ceyhan River basin (Turkey) with the use of aquatic macrophytes

As a major biotic component of many lotic ecosystems, macrophytes consist a major component of running waters are often used as indicators within the European Water Framework Directive (WFD) to establish ecological quality. In this study, we investigated macrophyte community structure (e.g. composition, abundance and diversity) in Ceyhan River Basin located in the Southeastern Anatolian Region in Turkey. Data was collected during 2014–2015 from river sites located throughout the basin to evaluate the relationship between aquatic vegetation and river physico-chemical factors. The ecological status of the river basin was also calculated based on Macrophyte Biological Index for River (IBMR). In total, 33 macrophyta taxa were observed. According to their biological classification (life form), filamentous algae (FA), free floating (FF), floating leaved (FL) and submersed (S) macrophytes reached their maximum abundance value in summer, while emergent (E) macrophytes were at their maximum abundance in both summer and autumn. The ecological status of the Ceyhan River basin ranged from moderate to bad. The values found are reasonably comparable to IBMR scores recorded in rivers of other Mediterranean countries. IBMR index may be suitable to some extent to establish a basis for ecological quality assessment in Turkish River systems.This research was financed by the Republic of Turkey Ministry of Agriculture and Forestry, through the project ‘Establishment of Ecological Assessment for Water Quality System in Turkey’

Modulation of mRNA expression and activities of xenobiotic metabolizing enzymes, CYP1A1, CYP1A2, CYP2E1, GPx and GSTP1 by the Salicornia freitagii extract in HT-29 human colon cancer cells

Phase I-II detoxification and antioxidant enzymes are responsible for the detoxification and elimination of activated carcinogens, acting as important biomarkers for chemoprevention. Among them, cytochrome P450s plays a prominent role in the metabolic activation of xenobiotics. The herb Salicornia freitagii (SF) (Amaranthaceae) is known for its anticancer, antioxidant, antidiabetic and antiinflammatory activities. In this study, we determined the bioactive phenolics in the SF methanol extract and investigated its antiproliferative potential in HT-29 human colon cancer cells. We also investigated the modulation of some phase I and II enzyme (CYP 1A1, 1A2, 2E1, GSTP1 and GPx) mRNA expression and enzymatic activities by the SF extract and its major bioactive phenolic compounds. LC/MS-MS analysis showed that the main phenolic compounds of the methanolic SF extract are vanillic acid (48 μg/100g) and p-coumaric acid (10.8 μg/100g). SF extract, vanillic acid and p-coumaric acid exhibited high antiproliferative activities in HT-29 cells, with IC50 values of 81.79μg/mL, 98.8 μM and 221.6 μM, respectively. The mRNA expression levels of CYP1A2 and CYP2E1 were decreased, while those of GSTP1 and GPx in HT-29 cells were increased after application of either the SF extract or vanillic acid. The SF extract by itself also increased the activities of GPx and GSTP1 enzymes 1.68- and 1.49-fold, respectively. Our data indicate that the SF extract and its major bioactive compound, vanillic acid, could exert a modulatory effect on the expression of enzymes that are involved in xenobiotic activation and detoxification pathways in the gastrointestinal tract. For this reason, SF can be considered as a natural source of chemopreventive agents

Modulation of mRNA expression and activities of xenobiotic metabolizing enzymes, CYP1A1, CYP1A2, CYP2E1, GPx and GSTP1 by the Salicornia freitagii extract in HT-29 human colon cancer cells

WOS:000408923400007Phase I-II detoxification and antioxidant enzymes are responsible for the detoxification and elimination of activated carcinogens, acting as important biomarkers for chemoprevention. Among them, cytochrome P450s plays a prominent role in the metabolic activation of xenobiotics. The herb Salicornia freitagii (SF) (Amaranthaceae) is known for its anticancer, antioxidant, antidiabetic and antiinflammatory activities. In this study, we determined the bioactive phenolics in the SF methanol extract and investigated its antiproliferative potential in HT-29 human colon cancer cells. We also investigated the modulation of some phase I and II enzyme (CYP 1A1, 1A2, 2E1, GSTP1 and GPx) mRNA expression and enzymatic activities by the SF extract and its major bioactive phenolic compounds. LC/MS-MS analysis showed that the main phenolic compounds of the methanolic SF extract are vanillic acid (48 mu g/100g) and p-coumaric acid (10.8 mu g/100g). SF extract, vanillic acid and p-coumaric acid exhibited high antiproliferative activities in HT-29 cells, with IC50 values of 81.79 mu g/mL, 98.8 mu M and 221.6 mu M, respectively. The mRNA expression levels of CYP1A2 and CYP2E1 were decreased, while those of GSTP1 and GPx in HT-29 cells were increased after application of either the SF extract or vanillic acid. The SF extract by itself also increased the activities of GPx and GSTP1 enzymes 1.68- and 1.49-fold, respectively. Our data indicate that the SF extract and its major bioactive compound, vanillic acid, could exert a modulatory effect on the expression of enzymes that are involved in xenobiotic activation and detoxification pathways in the gastrointestinal tract. For this reason, SF can be considered as a natural source of chemopreventive agents.Nezahat Gokyigit Botanical Garden in Turkey [60.05.02]; METU in Turkey [BAP-08-11-DPT-2011K121010]The authors express their gratitude to Associate Professor Dr. Gulcin Celep (Gazi University) who kindly collected and provided the samples for the study. This work was financially supported by Nezahat Gokyigit Botanical Garden (Research Project No. 60.05.02) in Turkey and METU (Project No. BAP-08-11-DPT-2011K121010) in Turkey

Glassworts as Possible Anticancer Agents Against Human Colorectal Adenocarcinoma Cells with Their Nutritive, Antioxidant and Phytochemical Profiles

In this study, the possible uses of glassworts as potential food ingredients and their antiproliferative activity against colorectal adenocarcinoma cells together with their antioxidant and phytochemical profiles were investigated for the first time. MeOH extracts of five different taxa collected from different localities were screened for their antioxidant capacities by DPPH (IC50 2.91-5.49mg/ml) and ABTS (24.4-38.5molTE/gextract) assays. Salicornia freitagii exhibited the highest DPPH radical scavenging activity. LC/MS/MS analysis displayed that vanillic acid and p-coumaric acid were two main phenolic compounds in the extract. Salicorniafreitagii extracts also exhibited high antiproliferative activity against HT-29 (IC50 1.67mg/ml) and Caco-2 (IC50 3.03mg/ml) cells for 72h. Mineral analysis indicated that all the species with different proportions of elemental components contained high amount of cations. These results indicate that investigated glassworts, with their high phenolic and mineral contents and also notable antioxidant and cytotoxic properties, may be utilized as a promising source of therapeutics