Pengaruh Waktu Polinasi dan Umur Polen terhadap Hasil Benih Terong Hijau (Solanum Melongena L.) Hibrida

Terong (Solanum melongena L.) merupakan tanaman sayuran yang potensial untuk dikembangkan di Indonesia. Pada tahun 2011 produktivitas terong di Indonesia 9,94 ton/ha, padahal potensi dapat mencapai 40 ton/ha. Upaya peningkatan produktivitas dilakukan dengan menyediakan benih terong hibrida. Kendala dalam pelaksanaan polinasi terong hibrida adalah waktu polinasi dan ketersediaan polen. Tujuan penelitian ini adalah mengetahui pengaruh interaksi antara waktu polinasi dan umur polen, serta mengetahui pengaruh penundaan waktu pollinasi dan umur polen terhadap hasil benih terong hijau hibrida. Penelitian dilaksanakan dengan menggunakan rancangan acak kelompok faktorial (RAKF) yang diulang 3 kali. Hasil penelitian menunjukkan bahwa interaksi antara waktu polinasi dan umur polen ditunjukkan pada bobot biji kering per buah, bobot biji kering per tanaman dan jumlah biji per buah. Penundaan waktu polinasi 24 jam setelah emaskulasi dan 48 jam setelah emaskulasi menghasilkan bobot biji kering per buah, bobot biji kering per tanaman dan jumlah biji per buah lebih banyak. Umur polen 2 hari menghasilkan bobot 1000 biji lebih tinggi sedangkan umur polen 0 hari dan 1 hari menghasilkan bobot biji kering per buah, bobot biji kering per tanaman dan jumlah biji per buah lebih banyak

Quantum Transport with Spin Dephasing: A Nonequilibrium Green's Function Approach

A quantum transport model incorporating spin scattering processes is presented using the non-equilibrium Green's function (NEGF) formalism within the self-consistent Born approximation. This model offers a unified approach by capturing the spin-flip scattering and the quantum effects simultaneously. A numerical implementation of the model is illustrated for magnetic tunnel junction devices with embedded magnetic impurity layers. The results are compared with experimental data, revealing the underlying physics of the coherent and incoherent transport regimes. It is shown that small variations in magnetic impurity spin-states/concentrations could cause large deviations in junction magnetoresistances.Comment: NEGF Formalism, Spin Dephasing, Magnetic Tunnel Junctions, Magnetoresistanc

Stopping Light All-Optically

We show that light pulses can be stopped and stored all-optically, with a process that involves an adiabatic and reversible pulse bandwidth compression occurring entirely in the optical domain. Such a process overcomes the fundamental bandwidth-delay constraint in optics, and can generate arbitrarily small group velocities for light pulses with a given bandwidth, without the use of any coherent or resonant light-matter interactions. We exhibit this process in optical resonator systems, where the pulse bandwidth compression is accomplished only by small refractive index modulations performed at moderate speeds. (Accepted for publication in Phys. Rev. Lett. Submitted on Sept. 10th 2003)Comment: 18 pages including 3 figures. Accepted for publication in Phys. Rev. Let

Electrokinetic confinement of axonal growth for dynamically configurable neural networks

Axons in the developing nervous system are directed via guidance cues, whose expression varies both spatially and temporally, to create functional neural circuits. Existing methods to create patterns of neural connectivity in vitro use only static geometries, and are unable to dynamically alter the guidance cues imparted on the cells. We introduce the use of AC electrokinetics to dynamically control axonal growth in cultured rat hippocampal neurons. We find that the application of modest voltages at frequencies on the order of 10[superscript 5] Hz can cause developing axons to be stopped adjacent to the electrodes while axons away from the electric fields exhibit uninhibited growth. By switching electrodes on or off, we can reversibly inhibit or permit axon passage across the electrodes. Our models suggest that dielectrophoresis is the causative AC electrokinetic effect. We make use of our dynamic control over axon elongation to create an axon-diode via an axon-lock system that consists of a pair of electrode ‘gates’ that either permit or prevent axons from passing through. Finally, we developed a neural circuit consisting of three populations of neurons, separated by three axon-locks to demonstrate the assembly of a functional, engineered neural network. Action potential recordings demonstrate that the AC electrokinetic effect does not harm axons, and Ca[superscript 2+] imaging demonstrated the unidirectional nature of the synaptic connections. AC electrokinetic confinement of axonal growth has potential for creating configurable, directional neural networks.National Institutes of Health (U.S.) (R01 EUREKA Award R01-NS066352

Transmission of Slow Light through Photonic Crystal Waveguide Bends

The spectral dependence of a bending loss of cascaded 60-degree bends in photonic crystal (PhC) waveguides is explored in a slab-type silicon-on-insulator system. Ultra-low bending loss of (0.05+/-0.03)dB/bend is measured at wavelengths corresponding to the nearly dispersionless transmission regime. In contrast, the PhC bend is found to become completely opaque for wavelengths range corresponding to the slow light regime. A general strategy is presented and experimentally verified to optimize the bend design for improved slow light transmission.Comment: 4 pages, 3 figures; submitted to Optics Letter

Ultra-rapid laser protein micropatterning: screening for directed polarization of single neurons

Protein micropatterning is a powerful tool for studying the effects of extracellular signals on cell development and regeneration. Laser micropatterning of proteins is the most flexible method for patterning many different geometries, protein densities, and concentration gradients. Despite these advantages, laser micropatterning remains prohibitively slow for most applications. Here, we take advantage of the rapid multi-photon induced photobleaching of fluorophores to generate sub-micron resolution patterns of full-length proteins on polymer monolayers, with sub-microsecond exposure times, i.e. one to five orders of magnitude faster than all previous laser micropatterning methods. We screened a range of different PEG monolayer coupling chemistries, chain-lengths and functional caps, and found that long-chain acrylated PEG monolayers are effective at resisting non-specific protein adhesion, while permitting efficient cross-linking of biotin-4-fluorescein to the PEG monolayers upon exposure to femtosecond laser pulses. We find evidence that the dominant photopatterning chemistry switches from a two-photon process to three- and four-photon absorption processes as the laser intensity increases, generating increasingly volatile excited triplet-state fluorophores, leading to faster patterning. Using this technology, we were able to generate over a hundred thousand protein patterns with varying geometries and protein densities to direct the polarization of hippocampal neurons with single-cell precision. We found that certain arrays of patterned triangles as small as neurite growth cones can direct polarization by impeding the elongation of reverse-projecting neurites, while permitting elongation of forward-projecting neurites. The ability to rapidly generate and screen such protein micropatterns can enable discovery of conditions necessary to create in vitro neural networks with single-neuron precision for basic discovery, drug screening, as well as for tissue scaffolding in therapeutics.Hertz Foundation (Fellowship)National Institutes of Health (U.S.) (R01 EUREKA Award 1-R01-NS066352)David & Lucile Packard Foundation (Award in Science and Engineering

The polypyrimidine tract-binding protein stimulates HIF-1α IRES-mediated translation during hypoxia

When oxygen supply is restricted, protein synthesis is rapidly abrogated owing to inhibition of global translation. However, HIF-1α protein expression can persist during hypoxia, owing to an internal ribosome entry site (IRES) in the 5′-untranslated region of its mRNA. Here, we report on the molecular mechanism of HIF-1α IRES-mediated translation during oxygen deprivation. Using RNA affinity chromatography and UV-crosslinking experiments, we show that the polypyrimidine tract binding protein (PTB) can specifically interact with the HIF-1α IRES, and that this interaction is enhanced in hypoxic conditions. Overexpression of PTB enhanced HIF-1α IRES activity, whereas RNA interference-mediated downregula-tion of PTB protein expression inhibited HIF-1α IRES activity. Furthermore, hypoxia-induced stimulation of the HIF-1α IRES was reduced in cells in which PTB function was downregulated. In agreement with these results, the IRES activity of HIF-1α IRES deletion mutants that are deficient in PTB-binding could not be stimulated by oxygen deprivation. All together, our data suggest that PTB plays a stimulatory role in the IRES-mediated translation of HIF-1α when oxygen supply is limited

UNR translation can be driven by an IRES element that is negatively regulated by polypyrimidine tract binding protein

Upstream of N-ras (Unr) has been described as an internal initiation trans-acting factor (ITAF) in the cap-independent translation of some particular viral and cellular mRNAs. Two factors led us to hypothesize that the UNR 5′-untranslated region (5′-UTR) may contain an internal ribosome entry site (IRES). The first was the requirement for persisting Unr expression under conditions that correlate with cap-independent translation. The other was the observation that the primary UNR transcript contains a 447 nt long 5′-UTR including two upstream AUGs that may restrict translation initiation via cap-dependent ribosome scanning. Here we report that the UNR 5′-UTR allows IRES-dependent translation, as revealed by a dicistronic reporter assay. Various controls ruled out the contribution of leaky scanning, cryptic promoter sequences or RNA processing events to the ability of the UNR 5′-UTR to mediate internal initiation of translation. Ultraviolet cross-linking analysis and RNA affinity chromatography revealed the binding of polypyrimidine tract binding protein (PTB) to the UNR IRES, requiring a pyrimidine-rich region (nucleotides 335–355). Whereas overexpression of PTB in several cell lines inhibited UNR IRES activity and UNR protein expression, depletion of endogenous PTB using RNAi increased UNR IRES activity. Moreover, a mutant version of the UNR IRES lacking the PTB binding site was more efficient at directing IRES-mediated translation. In conclusion, our results demonstrate that translation of the ITAF Unr can itself be regulated by an IRES that is downregulated by PTB

Waveguiding properties of surface states in photonic crystals

We propose and analyze novel surface-state-based waveguides in bandgap photonic crystals. We discuss surface mode band structure, field localization and effect of imperfections on the waveguiding properties of the surface modes. We demonstrate that surface-state-based waveguides can be used to achieve directional emission out of the waveguide. We also discuss the application of the surface-state waveguides as efficient light couplers for conventional photonic crystal waveguides.Comment: 4 pages 5 figure

Noninvasive electron microscopy with interaction-free quantum measurements

We propose the use of interaction-free quantum measurements with electrons to eliminate sample damage in electron microscopy. This might allow noninvasive molecular-resolution imaging. We show the possibility of such measurements in the presence of experimentally measured quantum decoherence rates and using a scheme based on existing charged particle trapping techniques.David and Lucile Packard Foundatio