Stabilization and Anticancer Enhancing Activity of the Peptide Nisin by Cyclodextrin-Based Nanosponges against Colon and Breast Cancer Cells

The great variability of cancer types demands novel drugs with broad spectrum, this is the case of Nisin, a polycyclic antibacterial peptide that recently has been considered for prevention of cancer cells growth. As an accepted food additive, this drug would be very useful for intestinal cancers, but the peptide nature would make easier its degradation by digestion procedures. For that reason, the aim of present study to investigate the protective effect of two different β-cyclodextrin-based nanosponges (carbonyl diimidazole and pyromellitic dianhydride) and their anti-cancer enhancement effect of Nisin-Z encapsulated with against colon cancer cells (HT-29). To extend its possible use, a comparison with breast (MCF-7) cancer cell was carried out. The physicochemical properties, loading efficiency, and release kinetics of Nisin complex with nanosponges were studied. Then, tricin-SDS-PAGE electrophoresis was used to understand the effect of NSs on stability of Nisin-Z in the presence of gastric peptidase pepsin. In addition, the cytotoxicity and cell membrane damage of Nisin Z were evaluated by using the MTT and LDH assay, which was complemented via Annexin-V/ Propidium Iodide (PI) by using flowcytometry. CD-NS are able to complex Nisin-Z with an encapsulation efficiency around 90%. A protective effect of Nisin-Z complexed with CD-NSs was observed in presence of pepsin. An increase in the percentage of apoptotic cells was observed when the cancer cells were exposed to Nisin Z complexed with nanosponges. Interestingly, Nisin Z free and loaded on PMDA/CDI-NSs is more selectively toxic towards HT-29 cells than MCF-7 cancer cells. These results indicated that nanosponges might be good candidates to protect peptides and deliver drugs against intestinal cancers

Psychometric properties of an instrument of reasons for admission to university careers in health sciences

[EN] The purpose of this research was to analyze the validity and reliability of the instrument called “Reasons for Admission to Careers in Health Sciences (MICCS) (acronym in Spanish)”. The study was developed with a non-probabilistic and incidental sample of 194 students from a higher education institution in the state of Jalisco, Mexico. After reviewing the contenty validity by expert judges, an exploratory factor analysis was executed (principal components method and Varimax rotation). Seven dimensions were defined under the critery of theoretical position and inflection of the curve of sedimentation. The indexes of KMO (0.788) and Bartlett (X (990) = 2916.36, p=0.000) reported an appropriate model. Items with theoretical inconsistencies and saturations under of 0.4 were eliminated. Its internal consistency was acceptable (α=0.834). The final version of the instrument consisted of 30 items and six independent factors, showing satisfactory psychometric properties. Finally were contrasted the reasons for entering careers in medicine, nursing, nutrition and dentistry to the variables of sex, vocational orientation and type of school. It is proposed to extend the investigation of reasons for entering science careers health by using the scale in public and private institutions of higher education; In addition, it is recommended to analyze the relationship of the reasons for admission and psychosocial variables such as academic performance, lag, desertion, satisfaction and academic stress.[ES] El presente trabajo de investigación tuvo como propósito analizar la validez y confiabilidad del instrumento denominado “Motivos de Ingreso a las Carreras de Ciencias de la Salud (MICCS)”. El estudio se desarrolló con una muestra no probabilística e incidental de 194 alumnos de una institución de educación superior del estado de Jalisco, México. Después de abordar la validez de contenido mediante jueces expertos, se ejecutó un análisis factorial exploratorio (método de componentes principales y rotación Varimax) donde se definieron siete dimensiones bajo el criterio de postura teórica e inflexión de la curva de sedimentación. Los índices de KMO (0.788) y Bartlett (X(990)=2916.36; p=0.000) señalaron un modelo apropiado y sin esfericidad. Los ítems con inconsistencias teóricas y saturaciones menores a 0.4 fueron eliminados. Su consistencia interna general fue aceptable (α=0.834). La versión final del instrumento quedó conformada de 30 reactivos y seis factores independientes, mostrando propiedades psicométricas satisfactorias. Por último, se contrastaron los motivos de ingreso de las carreras de medicina, enfermería, nutrición y odontología con las variables de sexo, orientación vocacional y tipo de bachillerato. Se propone ampliar la línea de investigación de los motivos de ingreso a carreras de ciencias de la salud mediante el uso de la escala en instituciones de educación superior públicas y privadas; además, se recomienda abordar la posible relación de los motivos de ingreso con variables escolares y psicosociales como el rendimiento académico, el atraso educativo, la deserción, la satisfacción y el estrés académico.Caldera Montes, JF.; Reynoso González, O.; Sughey González Torres, Y.; Zamora Betancourt, M. (2018). Propiedades psicométricas de un instrumento de motivos de ingreso a carreras universitarias de ciencias de la salud. 33-50. doi:10.4995/redu.2018.6052SWORD335

Coupled dark matter-dark energy in light of near Universe observations

Cosmological analysis based on currently available observations are unable to rule out a sizeable coupling among the dark energy and dark matter fluids. We explore a variety of coupled dark matter-dark energy models, which satisfy cosmic microwave background constraints, in light of low redshift and near universe observations. We illustrate the phenomenology of different classes of dark coupling models, paying particular attention in distinguishing between effects that appear only on the expansion history and those that appear in the growth of structure. We find that while a broad class of dark coupling models are effectively models where general relativity (GR) is modified --and thus can be probed by a combination of tests for the expansion history and the growth of structure--, there is a class of dark coupling models where gravity is still GR, but the growth of perturbations is, in principle modified. While this effect is small in the specific models we have considered, one should bear in mind that an inconsistency between reconstructed expansion history and growth may not uniquely indicate deviations from GR. Our low redshift constraints arise from cosmic velocities, redshift space distortions and dark matter abundance in galaxy voids. We find that current data constrain the dimensionless coupling to be |xi|<0.2, but prospects from forthcoming data are for a significant improvement. Future, precise measurements of the Hubble constant, combined with high-precision constraints on the growth of structure, could provide the key to rule out dark coupling models which survive other tests. We shall exploit as well weak equivalence principle violation arguments, which have the potential to highly disfavour a broad family of coupled models.Comment: 34 pages, 6 figures; changes to match published versio

The generalized second law for the interacting generalized Chaplygin gas model

We investigate the validity of the generalized second law (GSL) of gravitational thermodynamics in a non-flat FRW universe containing the interacting generalized Chaplygin gas with the baryonic matter. The dynamical apparent horizon is assumed to be the boundary of the universe. We show that for the interacting generalized Chaplygin gas as a unified candidate for dark matter (DM) and dark energy (DE), the equation of state parameter can cross the phantom divide. We also present that for the selected model under thermal equilibrium with the Hawking radiation, the GSL is always satisfied throughout the history of the universe for any spatial curvature, independently of the equation of state of the interacting generalized Chaplygin gas model.Comment: 8 page

Interacting entropy-corrected new agegraphic dark energy in Brans-Dicke cosmology

Motivated by a recent work of one of us [1], we extend it by using quantum (or entropy) corrected new agegraphic dark energy in the Brans-Dicke cosmology. The correction terms are motivated from the loop quantum gravity which is one of the competitive theories of quantum gravity. Taking the non-flat background spacetime along with the conformal age of the universe as the length scale, we derive the dynamical equation of state of dark energy and the deceleration parameter. An important consequence of this study is the phantom divide scenario with entropy-corrected new agegraphic dark energy. Moreover, we assume a system of dark matter, radiation and dark energy, while the later interacts only with dark matter. We obtain some essential expressions related with dark energy dynamics. The cosmic coincidence problem is also resolved in our model.Comment: 16 pages, no figure, accepted for publication in Gen. Relativ. Gra

Large-scale instability in interacting dark energy and dark matter fluids

If dark energy interacts with dark matter, this gives a new approach to the coincidence problem. But interacting dark energy models can suffer from pathologies. We consider the case where the dark energy is modelled as a fluid with constant equation of state parameter w. Non-interacting constant-w models are well behaved in the background and in the perturbed universe. But the combination of constant w and a simple interaction with dark matter leads to an instability in the dark sector perturbations at early times: the curvature perturbation blows up on super-Hubble scales. Our results underline how important it is to carefully analyze the relativistic perturbations when considering models of coupled dark energy. The instability that we find has been missed in some previous work where the perturbations were not consistently treated. The unstable mode dominates even if adiabatic initial conditions are used. The instability also arises regardless of how weak the coupling is. This non-adiabatic instability is different from previously discovered adiabatic instabilities on small scales in the strong-coupling regime.Comment: 15 pages, 5 figures. New reference; published versio

Large-scale instability in interacting dark energy and dark matter fluids

If dark energy interacts with dark matter, this gives a new approach to the coincidence problem. But interacting dark energy models can suffer from pathologies. We consider the case where the dark energy is modelled as a fluid with constant equation of state parameter w. Non-interacting constant-w models are well behaved in the background and in the perturbed universe. But the combination of constant w and a simple interaction with dark matter leads to an instability in the dark sector perturbations at early times: the curvature perturbation blows up on super-Hubble scales. Our results underline how important it is to carefully analyze the relativistic perturbations when considering models of coupled dark energy. The instability that we find has been missed in some previous work where the perturbations were not consistently treated. The unstable mode dominates even if adiabatic initial conditions are used. The instability also arises regardless of how weak the coupling is. This non-adiabatic instability is different from previously discovered adiabatic instabilities on small scales in the strong-coupling regime.Comment: 15 pages, 5 figures. New reference; published versio

Notes on interacting holographic dark energy model in a closed universe

We consider interacting holographic dark energy model in Friedmann Robertson Walker space time with positive spatial curvature and investigate the behavior of curvature parameter and dark energy density in accelerated expanding epoch. We also derive some conditions needed to cross the phantom divide line in this model.Comment: 10 pages, typos corrected, some explanations and references added and updated, accepted for publication in JCA

REST Controls Self-Renewal and Tumorigenic Competence of Human Glioblastoma Cells

The Repressor Element 1 Silencing Transcription factor (REST/NRSF) is a master repressor of neuronal programs in non-neuronal lineages shown to function as a central regulator of developmental programs and stem cell physiology. Aberrant REST function has been associated with a number of pathological conditions. In cancer biology, REST has been shown to play a tumor suppressor activity in epithelial cancers but an oncogenic role in brain childhood malignancies such as neuroblastoma and medulloblastoma. Here we examined REST expression in human glioblastoma multiforme (GBM) specimens and its role in GBM cells carrying self-renewal and tumorigenic competence. We found REST to be expressed in GBM specimens, its presence being particularly enriched in tumor cells in the perivascular compartment. Significantly, REST is highly expressed in self-renewing tumorigenic-competent GBM cells and its knock down strongly reduces their self-renewal in vitro and tumor-initiating capacity in vivo and affects levels of miR-124 and its downstream targets. These results indicate that REST contributes to GBM maintenance by affecting its self-renewing and tumorigenic cellular component and that, hence, a better understanding of these circuitries in these cells might lead to new exploitable therapeutic targets